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Alternative name: MED1
Polyclonal antibody raised in rabbit against human MBD4 (Methyl-CpG-binding domain protein 4), using three different KLH-conjugated synthetic peptides.
| Lot | A129-004 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 - 1:1000 | Fig 1 |
| Western Blotting | 1:2,000 | Fig 2 |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD4 (Cat. No. CS-087-100) in antigen coated wells. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against MBD4
Figure 2A: Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MBD4 (Cat. No. CS- 087-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder.
Figure 2B: Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 μg) with the antibody against MBD4, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| Datasheet MBD4 CS-087-100 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: MBD4 polyclonal antibody (Diagenode Cat# C15310087 Lot# A129-004 ). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'MBD4 polyclonal antibody - Pioneer', 'meta_title' => 'MBD4 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2079', 'antibody_id' => '270', 'name' => 'MBD4 polyclonal antibody ', 'description' => '<p><span>Alternative name: <strong>MED1</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human MBD4 (Methyl-CpG-binding domain protein 4), using three different KLH-conjugated synthetic peptides.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310087_fig1.jpg" alt="ELISA" height="284" width="337" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD4 (Cat. No. CS-087-100) in antigen coated wells. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310087_fig2.jpg" alt="Western blot" height="239" width="342" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD4<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MBD4. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'MBD4 polyclonal antibody - Pioneer', 'meta_title' => 'MBD4 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2079', 'antibody_id' => '270', 'name' => 'MBD4 polyclonal antibody ', 'description' => '<p><span>Alternative name: <strong>MED1</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human MBD4 (Methyl-CpG-binding domain protein 4), using three different KLH-conjugated synthetic peptides.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310087_fig1.jpg" alt="ELISA" height="284" width="337" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD4 (Cat. No. CS-087-100) in antigen coated wells. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310087_fig2.jpg" alt="Western blot" height="239" width="342" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD4<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MBD4 (Cat. No. CS- 087-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 μg) with the antibody against MBD4, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. 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Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD4 (Cat. No. CS-087-100) in antigen coated wells. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310087_fig2.jpg" alt="Western blot" height="239" width="342" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD4<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MBD4 (Cat. No. CS- 087-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 μg) with the antibody against MBD4, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310087', 'old_catalog_number' => 'CS-087-100', 'sf_code' => 'C15310087-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'mbd4-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'MBD4 polyclonal antibody - Pioneer', 'meta_keywords' => '', 'meta_description' => 'MBD4 polyclonal antibody - Pioneer', 'modified' => '2022-01-05 16:03:22', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '270', 'name' => 'Mbd4 polyclonal antibody', 'description' => 'MBD4 (UniProt/Swiss-Prot entry O95243) belongs to the family of methyl CpG binding proteins that specifically bind to methylated CpG dinucleotides. 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Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'MBD4 polyclonal antibody - Pioneer', 'meta_title' => 'MBD4 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2079', 'antibody_id' => '270', 'name' => 'MBD4 polyclonal antibody ', 'description' => '<p><span>Alternative name: <strong>MED1</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human MBD4 (Methyl-CpG-binding domain protein 4), using three different KLH-conjugated synthetic peptides.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310087_fig1.jpg" alt="ELISA" height="284" width="337" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD4 (Cat. No. CS-087-100) in antigen coated wells. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310087_fig2.jpg" alt="Western blot" height="239" width="342" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD4<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MBD4 (Cat. No. CS- 087-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 μg) with the antibody against MBD4, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310087', 'old_catalog_number' => 'CS-087-100', 'sf_code' => 'C15310087-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'mbd4-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'MBD4 polyclonal antibody - Pioneer', 'meta_keywords' => '', 'meta_description' => 'MBD4 polyclonal antibody - Pioneer', 'modified' => '2022-01-05 16:03:22', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '270', 'name' => 'Mbd4 polyclonal antibody', 'description' => 'MBD4 (UniProt/Swiss-Prot entry O95243) belongs to the family of methyl CpG binding proteins that specifically bind to methylated CpG dinucleotides. 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Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD4 (Cat. No. CS-087-100) in antigen coated wells. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310087_fig2.jpg" alt="Western blot" height="239" width="342" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD4<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MBD4 (Cat. No. CS- 087-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 μg) with the antibody against MBD4, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310087', 'old_catalog_number' => 'CS-087-100', 'sf_code' => 'C15310087-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'mbd4-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'MBD4 polyclonal antibody - Pioneer', 'meta_keywords' => '', 'meta_description' => 'MBD4 polyclonal antibody - Pioneer', 'modified' => '2022-01-05 16:03:22', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '270', 'name' => 'Mbd4 polyclonal antibody', 'description' => 'MBD4 (UniProt/Swiss-Prot entry O95243) belongs to the family of methyl CpG binding proteins that specifically bind to methylated CpG dinucleotides. 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'MBD4 polyclonal antibody - Pioneer', 'meta_title' => 'MBD4 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2079', 'antibody_id' => '270', 'name' => 'MBD4 polyclonal antibody ', 'description' => '<p><span>Alternative name: <strong>MED1</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human MBD4 (Methyl-CpG-binding domain protein 4), using three different KLH-conjugated synthetic peptides.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310087_fig1.jpg" alt="ELISA" height="284" width="337" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD4 (Cat. No. CS-087-100) in antigen coated wells. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310087_fig2.jpg" alt="Western blot" height="239" width="342" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD4<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MBD4 (Cat. No. CS- 087-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 μg) with the antibody against MBD4, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310087', 'old_catalog_number' => 'CS-087-100', 'sf_code' => 'C15310087-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'mbd4-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'MBD4 polyclonal antibody - Pioneer', 'meta_keywords' => '', 'meta_description' => 'MBD4 polyclonal antibody - Pioneer', 'modified' => '2022-01-05 16:03:22', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '270', 'name' => 'Mbd4 polyclonal antibody', 'description' => 'MBD4 (UniProt/Swiss-Prot entry O95243) belongs to the family of methyl CpG binding proteins that specifically bind to methylated CpG dinucleotides. MBD4 probably functions to mediate the biological consequences of CpG methylation. Further, MBD4 has thymine glycosylase activity and is involved in DNA repair. Mutations of MBD4 are associated with tumors with primary microsatellite-instability, a type of genomic instability that is caused by a defective DNA mismatch repair.', 'clonality' => '', 'isotype' => '', 'lot' => 'A129-004 ', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100 - 1:1000</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:2,000</td> <td>Fig 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 14:59:38', 'created' => '0000-00-00 00:00:00', 'select_label' => '270 - Mbd4 polyclonal antibody (A129-004 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'MBD4 polyclonal antibody - Pioneer' $meta_title = 'MBD4 polyclonal antibody - Pioneer' $product = array( 'Product' => array( 'id' => '2079', 'antibody_id' => '270', 'name' => 'MBD4 polyclonal antibody ', 'description' => '<p><span>Alternative name: <strong>MED1</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human MBD4 (Methyl-CpG-binding domain protein 4), using three different KLH-conjugated synthetic peptides.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310087_fig1.jpg" alt="ELISA" height="284" width="337" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD4 (Cat. No. CS-087-100) in antigen coated wells. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.</small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310087_fig2.jpg" alt="Western blot" height="239" width="342" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD4<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MBD4 (Cat. No. CS- 087-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 μg) with the antibody against MBD4, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310087', 'old_catalog_number' => 'CS-087-100', 'sf_code' => 'C15310087-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'mbd4-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'MBD4 polyclonal antibody - Pioneer', 'meta_keywords' => '', 'meta_description' => 'MBD4 polyclonal antibody - Pioneer', 'modified' => '2022-01-05 16:03:22', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '270', 'name' => 'Mbd4 polyclonal antibody', 'description' => 'MBD4 (UniProt/Swiss-Prot entry O95243) belongs to the family of methyl CpG binding proteins that specifically bind to methylated CpG dinucleotides. MBD4 probably functions to mediate the biological consequences of CpG methylation. Further, MBD4 has thymine glycosylase activity and is involved in DNA repair. Mutations of MBD4 are associated with tumors with primary microsatellite-instability, a type of genomic instability that is caused by a defective DNA mismatch repair.', 'clonality' => '', 'isotype' => '', 'lot' => 'A129-004 ', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100 - 1:1000</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:2,000</td> <td>Fig 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 14:59:38', 'created' => '0000-00-00 00:00:00', 'select_label' => '270 - Mbd4 polyclonal antibody (A129-004 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. 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ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. 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