MBD4 Antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Antibodies

Catalog Number

Format

Price

C15310087
(CS-087-100)

100 µl

$260.00
Bulk order

Alternative name: MED1

Polyclonal antibody raised in rabbit against human MBD4 (Methyl-CpG-binding domain protein 4), using three different KLH-conjugated synthetic peptides.

Lot	A129-004
Concentration	not determined
Species reactivity	Human
Type	Polyclonal
Purity	Whole antiserum
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:100 - 1:1000	Fig 1
Western Blotting	1:2,000	Fig 2

Validation Data

Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD4 (Cat. No. CS-087-100) in antigen coated wells. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.

Figure 2. Western blot analysis using the Diagenode antibody directed against MBD4
Figure 2A: Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MBD4 (Cat. No. CS- 087-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder.
Figure 2B: Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 μg) with the antibody against MBD4, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.

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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD4<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MBD4 (Cat. No. CS- 087-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 &mu;g) with the antibody against MBD4, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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<th>References</th>
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<td>Fig 1</td>
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<p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD4 (Cat. No. CS-087-100) in antigen coated wells. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD4<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MBD4 (Cat. No. CS- 087-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 &mu;g) with the antibody against MBD4, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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<td>Fig 1</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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			'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
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	'meta_description' => 'MBD4 (Methyl-CpG-binding domain protein 4) Polyclonal Antibody  validated  in  WB and ELISA. Batch-specific data available on the website. Alternative name: MED1',
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<p><span>Polyclonal antibody raised in rabbit against human MBD4 (Methyl-CpG-binding domain protein 4), using three different KLH-conjugated synthetic peptides.</span></p>',
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310087_fig1.jpg" alt="ELISA" height="284" width="337" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD4 (Cat. No. CS-087-100) in antigen coated wells. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310087_fig2.jpg" alt="Western blot" height="239" width="342" /></p>
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<div class="small-6 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD4<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MBD4 (Cat. No. CS- 087-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 &mu;g) with the antibody against MBD4, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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<p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MBD4 (Cat. No. CS-087-100) in antigen coated wells. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MBD4<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MBD4 (Cat. No. CS- 087-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 &mu;g) with the antibody against MBD4, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<th>References</th>
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<td>Fig 1</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<li>Batch-specific data is available on the website</li>
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<li>Cost-effective (requires less antibody per reaction)</li>
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Datasheet MBD4 CS-087-100 DATASHEET Datasheet description	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

MBD4 Antibody SDS GB en	Download
MBD4 Antibody SDS US en	Download
MBD4 Antibody SDS DE de	Download
MBD4 Antibody SDS JP ja	Download
MBD4 Antibody SDS BE nl	Download
MBD4 Antibody SDS BE fr	Download
MBD4 Antibody SDS FR fr	Download
MBD4 Antibody SDS ES es	Download

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