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Alternative names: AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT
Polyclonal antibody raised in rabbit against human MeCP2 (Methyl-CpG-binding domain protein 2), using 3 different KLH-conjugated synthetic peptides containing an amino acid sequence from the N-terminal, the central and the C-terminal part of the protein, respectively.
| Lot | A131-004 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 - 1:500 | Fig 1 |
| Western Blotting | 1:2,000 | Fig 2 |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MeCP2 (Cat. No. CS-088-100), in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2
Figure 2A: Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MeCP2. The presence of the TY1-MeCP2 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MeCP2 (Cat. No. CS-088-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder.
Figure 2B: Western blot was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 μg) with the Diagenode antibody against human MeCP2 (Cat. No. CS-088-100), diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| Datasheet MeCP2 CS-088-100 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: MeCP2 polyclonal antibody (Diagenode Cat# C15310088 Lot# A131-004 ). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'MeCP2 polyclonal antibody - Pioneer', 'meta_title' => 'MeCP2 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2080', 'antibody_id' => '205', 'name' => 'MeCP2 polyclonal antibody ', 'description' => '<p><span>Alternative names: <strong>AUTSX3</strong>, <strong>MRX16</strong>, <strong>MRX79</strong>, <strong>MRXS13</strong>, <strong>MRXSL</strong>, <strong>PPMX</strong>, <strong>RTS</strong>, <strong>RTT</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human MeCP2 (Methyl-CpG-binding domain protein 2), using 3 different KLH-conjugated synthetic peptides containing an amino acid sequence from the N-terminal, the central and the C-terminal part of the protein, respectively.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310088_fig1.jpg" alt="ELISA" height="306" width="341" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MeCP2 (Cat. No. CS-088-100), in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000. </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310088_fig2.jpg" alt="Western blot" height="235" width="365" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MeCP2. The presence of the TY1-MeCP2 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MeCP2 (Cat. No. CS-088-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 μg) with the Diagenode antibody against human MeCP2 (Cat. No. CS-088-100), diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. 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It belongs to the family of methyl CpG binding proteins which also comprises MBD1, MBD2, MBD3 and MBD4. MeCP2 can bind specifically to methylated promoters, thereby repressing transcription. This transcriptional repression is mediated through interaction with histone deacetylase and the corepressor SIN3A. MeCP2 also is essential for development. Mutations in MeCP2 are the cause of several types of mental retardation including Rett syndrome, a progressive neurological disorder that causes mental retardation in females, and mental retardation syndromic X-linked type 13, and may also be involved in Angelman syndrome and susceptibility to some types of autism.', 'clonality' => '', 'isotype' => '', 'lot' => 'A131-004 ', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100 - 1:500</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:2,000</td> <td>Fig 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 12:00:44', 'created' => '0000-00-00 00:00:00', 'select_label' => '205 - MeCP2 polyclonal antibody (A131-004 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'MeCP2 polyclonal antibody - Pioneer' $meta_title = 'MeCP2 polyclonal antibody - Pioneer' $product = array( 'Product' => array( 'id' => '2080', 'antibody_id' => '205', 'name' => 'MeCP2 polyclonal antibody ', 'description' => '<p><span>Alternative names: <strong>AUTSX3</strong>, <strong>MRX16</strong>, <strong>MRX79</strong>, <strong>MRXS13</strong>, <strong>MRXSL</strong>, <strong>PPMX</strong>, <strong>RTS</strong>, <strong>RTT</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human MeCP2 (Methyl-CpG-binding domain protein 2), using 3 different KLH-conjugated synthetic peptides containing an amino acid sequence from the N-terminal, the central and the C-terminal part of the protein, respectively.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310088_fig1.jpg" alt="ELISA" height="306" width="341" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MeCP2 (Cat. No. CS-088-100), in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000. </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310088_fig2.jpg" alt="Western blot" height="235" width="365" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MeCP2. The presence of the TY1-MeCP2 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MeCP2 (Cat. No. CS-088-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 μg) with the Diagenode antibody against human MeCP2 (Cat. No. CS-088-100), diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310088', 'old_catalog_number' => 'CS-088-100', 'sf_code' => 'C15310088-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'mecp2-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'MeCP2 polyclonal antibody - Pioneer', 'meta_keywords' => '', 'meta_description' => 'MeCP2 polyclonal antibody - Pioneer', 'modified' => '2022-01-05 16:03:07', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '205', 'name' => 'MeCP2 polyclonal antibody', 'description' => 'MeCP2 (UniProt/Swiss-Prot entry P51608) is a chromosomal protein with abundant binding sites in chromatin. 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Mutations in MeCP2 are the cause of several types of mental retardation including Rett syndrome, a progressive neurological disorder that causes mental retardation in females, and mental retardation syndromic X-linked type 13, and may also be involved in Angelman syndrome and susceptibility to some types of autism.', 'clonality' => '', 'isotype' => '', 'lot' => 'A131-004 ', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100 - 1:500</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:2,000</td> <td>Fig 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 12:00:44', 'created' => '0000-00-00 00:00:00', 'select_label' => '205 - MeCP2 polyclonal antibody (A131-004 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '18', 'position' => '20', 'parent_id' => '4', 'name' => 'Chromatin associated proteins', 'description' => '<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '590', 'name' => 'Datasheet MeCP2 CS-088-100', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_MeCP2_CS-088-100.pdf', 'slug' => 'datasheet-mecp2-cs-088-100', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'MeCP2 polyclonal antibody - Pioneer', 'meta_title' => 'MeCP2 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2080', 'antibody_id' => '205', 'name' => 'MeCP2 polyclonal antibody ', 'description' => '<p><span>Alternative names: <strong>AUTSX3</strong>, <strong>MRX16</strong>, <strong>MRX79</strong>, <strong>MRXS13</strong>, <strong>MRXSL</strong>, <strong>PPMX</strong>, <strong>RTS</strong>, <strong>RTT</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human MeCP2 (Methyl-CpG-binding domain protein 2), using 3 different KLH-conjugated synthetic peptides containing an amino acid sequence from the N-terminal, the central and the C-terminal part of the protein, respectively.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310088_fig1.jpg" alt="ELISA" height="306" width="341" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MeCP2 (Cat. No. CS-088-100), in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000. </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310088_fig2.jpg" alt="Western blot" height="235" width="365" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MeCP2. The presence of the TY1-MeCP2 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MeCP2 (Cat. No. CS-088-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 μg) with the Diagenode antibody against human MeCP2 (Cat. No. CS-088-100), diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. 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Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MeCP2 (Cat. No. CS-088-100), in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000. </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310088_fig2.jpg" alt="Western blot" height="235" width="365" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MeCP2. The presence of the TY1-MeCP2 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MeCP2 (Cat. No. CS-088-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 μg) with the Diagenode antibody against human MeCP2 (Cat. No. CS-088-100), diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. 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Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '590', 'name' => 'Datasheet MeCP2 CS-088-100', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_MeCP2_CS-088-100.pdf', 'slug' => 'datasheet-mecp2-cs-088-100', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'MeCP2 polyclonal antibody - Pioneer', 'meta_title' => 'MeCP2 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2080', 'antibody_id' => '205', 'name' => 'MeCP2 polyclonal antibody ', 'description' => '<p><span>Alternative names: <strong>AUTSX3</strong>, <strong>MRX16</strong>, <strong>MRX79</strong>, <strong>MRXS13</strong>, <strong>MRXSL</strong>, <strong>PPMX</strong>, <strong>RTS</strong>, <strong>RTT</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human MeCP2 (Methyl-CpG-binding domain protein 2), using 3 different KLH-conjugated synthetic peptides containing an amino acid sequence from the N-terminal, the central and the C-terminal part of the protein, respectively.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310088_fig1.jpg" alt="ELISA" height="306" width="341" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MeCP2 (Cat. No. CS-088-100), in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000. </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310088_fig2.jpg" alt="Western blot" height="235" width="365" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MeCP2. The presence of the TY1-MeCP2 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MeCP2 (Cat. No. CS-088-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 μg) with the Diagenode antibody against human MeCP2 (Cat. No. CS-088-100), diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310088', 'old_catalog_number' => 'CS-088-100', 'sf_code' => 'C15310088-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'mecp2-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'MeCP2 polyclonal antibody - Pioneer', 'meta_keywords' => '', 'meta_description' => 'MeCP2 polyclonal antibody - Pioneer', 'modified' => '2022-01-05 16:03:07', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '205', 'name' => 'MeCP2 polyclonal antibody', 'description' => 'MeCP2 (UniProt/Swiss-Prot entry P51608) is a chromosomal protein with abundant binding sites in chromatin. It belongs to the family of methyl CpG binding proteins which also comprises MBD1, MBD2, MBD3 and MBD4. MeCP2 can bind specifically to methylated promoters, thereby repressing transcription. This transcriptional repression is mediated through interaction with histone deacetylase and the corepressor SIN3A. MeCP2 also is essential for development. Mutations in MeCP2 are the cause of several types of mental retardation including Rett syndrome, a progressive neurological disorder that causes mental retardation in females, and mental retardation syndromic X-linked type 13, and may also be involved in Angelman syndrome and susceptibility to some types of autism.', 'clonality' => '', 'isotype' => '', 'lot' => 'A131-004 ', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100 - 1:500</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:2,000</td> <td>Fig 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MeCP2 (Cat. No. CS-088-100), in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000. </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310088_fig2.jpg" alt="Western blot" height="235" width="365" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MeCP2. The presence of the TY1-MeCP2 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MeCP2 (Cat. No. CS-088-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 μg) with the Diagenode antibody against human MeCP2 (Cat. No. CS-088-100), diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'MeCP2 polyclonal antibody - Pioneer', 'meta_title' => 'MeCP2 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2080', 'antibody_id' => '205', 'name' => 'MeCP2 polyclonal antibody ', 'description' => '<p><span>Alternative names: <strong>AUTSX3</strong>, <strong>MRX16</strong>, <strong>MRX79</strong>, <strong>MRXS13</strong>, <strong>MRXSL</strong>, <strong>PPMX</strong>, <strong>RTS</strong>, <strong>RTT</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human MeCP2 (Methyl-CpG-binding domain protein 2), using 3 different KLH-conjugated synthetic peptides containing an amino acid sequence from the N-terminal, the central and the C-terminal part of the protein, respectively.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310088_fig1.jpg" alt="ELISA" height="306" width="341" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MeCP2 (Cat. No. CS-088-100), in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000. </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310088_fig2.jpg" alt="Western blot" height="235" width="365" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MeCP2. The presence of the TY1-MeCP2 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MeCP2 (Cat. No. CS-088-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 μg) with the Diagenode antibody against human MeCP2 (Cat. No. CS-088-100), diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310088', 'old_catalog_number' => 'CS-088-100', 'sf_code' => 'C15310088-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'mecp2-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'MeCP2 polyclonal antibody - Pioneer', 'meta_keywords' => '', 'meta_description' => 'MeCP2 polyclonal antibody - Pioneer', 'modified' => '2022-01-05 16:03:07', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '205', 'name' => 'MeCP2 polyclonal antibody', 'description' => 'MeCP2 (UniProt/Swiss-Prot entry P51608) is a chromosomal protein with abundant binding sites in chromatin. It belongs to the family of methyl CpG binding proteins which also comprises MBD1, MBD2, MBD3 and MBD4. MeCP2 can bind specifically to methylated promoters, thereby repressing transcription. This transcriptional repression is mediated through interaction with histone deacetylase and the corepressor SIN3A. MeCP2 also is essential for development. Mutations in MeCP2 are the cause of several types of mental retardation including Rett syndrome, a progressive neurological disorder that causes mental retardation in females, and mental retardation syndromic X-linked type 13, and may also be involved in Angelman syndrome and susceptibility to some types of autism.', 'clonality' => '', 'isotype' => '', 'lot' => 'A131-004 ', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100 - 1:500</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:2,000</td> <td>Fig 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 12:00:44', 'created' => '0000-00-00 00:00:00', 'select_label' => '205 - MeCP2 polyclonal antibody (A131-004 - not determined - Human - Whole antiserum - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'MeCP2 polyclonal antibody - Pioneer' $meta_title = 'MeCP2 polyclonal antibody - Pioneer' $product = array( 'Product' => array( 'id' => '2080', 'antibody_id' => '205', 'name' => 'MeCP2 polyclonal antibody ', 'description' => '<p><span>Alternative names: <strong>AUTSX3</strong>, <strong>MRX16</strong>, <strong>MRX79</strong>, <strong>MRXS13</strong>, <strong>MRXSL</strong>, <strong>PPMX</strong>, <strong>RTS</strong>, <strong>RTT</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against human MeCP2 (Methyl-CpG-binding domain protein 2), using 3 different KLH-conjugated synthetic peptides containing an amino acid sequence from the N-terminal, the central and the C-terminal part of the protein, respectively.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310088_fig1.jpg" alt="ELISA" height="306" width="341" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MeCP2 (Cat. No. CS-088-100), in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000. </small></p> </div> </div> <div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310088_fig2.jpg" alt="Western blot" height="235" width="365" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MeCP2. The presence of the TY1-MeCP2 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MeCP2 (Cat. No. CS-088-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 μg) with the Diagenode antibody against human MeCP2 (Cat. No. CS-088-100), diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310088', 'old_catalog_number' => 'CS-088-100', 'sf_code' => 'C15310088-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'mecp2-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'MeCP2 polyclonal antibody - Pioneer', 'meta_keywords' => '', 'meta_description' => 'MeCP2 polyclonal antibody - Pioneer', 'modified' => '2022-01-05 16:03:07', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '205', 'name' => 'MeCP2 polyclonal antibody', 'description' => 'MeCP2 (UniProt/Swiss-Prot entry P51608) is a chromosomal protein with abundant binding sites in chromatin. It belongs to the family of methyl CpG binding proteins which also comprises MBD1, MBD2, MBD3 and MBD4. MeCP2 can bind specifically to methylated promoters, thereby repressing transcription. This transcriptional repression is mediated through interaction with histone deacetylase and the corepressor SIN3A. MeCP2 also is essential for development. Mutations in MeCP2 are the cause of several types of mental retardation including Rett syndrome, a progressive neurological disorder that causes mental retardation in females, and mental retardation syndromic X-linked type 13, and may also be involved in Angelman syndrome and susceptibility to some types of autism.', 'clonality' => '', 'isotype' => '', 'lot' => 'A131-004 ', 'concentration' => 'not determined', 'reactivity' => 'Human', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100 - 1:500</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:2,000</td> <td>Fig 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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