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<p><span>Polyclonal antibody raised in rabbit against human MeCP2 (Methyl-CpG-binding domain protein 2), using 3 different KLH-conjugated synthetic peptides containing an amino acid sequence from the N-terminal, the central and the C-terminal part of the protein, respectively.</span></p>',
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<p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MeCP2 (Cat. No. CS-088-100), in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MeCP2. The presence of the TY1-MeCP2 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MeCP2 (Cat. No. CS-088-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 μg) with the Diagenode antibody against human MeCP2 (Cat. No. CS-088-100), diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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<p><span>Polyclonal antibody raised in rabbit against human MeCP2 (Methyl-CpG-binding domain protein 2), using 3 different KLH-conjugated synthetic peptides containing an amino acid sequence from the N-terminal, the central and the C-terminal part of the protein, respectively.</span></p>',
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MeCP2. The presence of the TY1-MeCP2 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MeCP2 (Cat. No. CS-088-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 μg) with the Diagenode antibody against human MeCP2 (Cat. No. CS-088-100), diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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include - APP/View/Products/view.ctp, line 755
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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View::render() - CORE/Cake/View/View.php, line 473
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
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'description' => '<p><span>Alternative names: <strong>AUTSX3</strong>, <strong>MRX16</strong>, <strong>MRX79</strong>, <strong>MRXS13</strong>, <strong>MRXSL</strong>, <strong>PPMX</strong>, <strong>RTS</strong>, <strong>RTT</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human MeCP2 (Methyl-CpG-binding domain protein 2), using 3 different KLH-conjugated synthetic peptides containing an amino acid sequence from the N-terminal, the central and the C-terminal part of the protein, respectively.</span></p>',
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<div class="small-6 columns">
<p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MeCP2 (Cat. No. CS-088-100), in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MeCP2. The presence of the TY1-MeCP2 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MeCP2 (Cat. No. CS-088-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 μg) with the Diagenode antibody against human MeCP2 (Cat. No. CS-088-100), diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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<p><span>Polyclonal antibody raised in rabbit against human MeCP2 (Methyl-CpG-binding domain protein 2), using 3 different KLH-conjugated synthetic peptides containing an amino acid sequence from the N-terminal, the central and the C-terminal part of the protein, respectively.</span></p>',
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<p><small><strong>Figure 1. Determination of the titer<br /></strong> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MeCP2 (Cat. No. CS-088-100), in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against MeCP2<br /></strong> <strong>Figure 2A:</strong> Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MeCP2. The presence of the TY1-MeCP2 in the cell lysates was demonstrated by western blot analysis with the Diagenode antibody against the TY1-tag (Cat. No. MAb-054-050) (lane 1) and with the antibody against MeCP2 (Cat. No. CS-088-100) (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. <br /><strong>Figure 2B:</strong> Western blot was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 μg) with the Diagenode antibody against human MeCP2 (Cat. No. CS-088-100), diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.</small></p>
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'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'ProductsApplication' => array(
'id' => '3132',
'product_id' => '2080',
'application_id' => '19'
)
)
$slugs = array(
(int) 0 => 'western-blot-antibodies'
)
$applications = array(
'id' => '19',
'position' => '10',
'parent_id' => '40',
'name' => 'WB',
'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'western-blot-antibodies',
'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-04-26 12:44:51',
'created' => '2015-01-07 09:20:00',
'locale' => 'zho'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
'id' => '1564',
'product_id' => '2080',
'document_id' => '38'
)
)
$sds = array(
'id' => '2636',
'name' => 'MeCP2 Antibody SDS ES es',
'language' => 'es',
'url' => 'files/SDS/MeCP2/SDS-C15310088-MeCP2_Antibody-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2022-10-03 11:42:22',
'created' => '2022-10-03 11:42:22',
'ProductsSafetySheet' => array(
'id' => '4444',
'product_id' => '2080',
'safety_sheet_id' => '2636'
)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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