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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed on MV4-11 cells using the Diagenode antibody against MLL1 (Cat. No. C15310264). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 700 kb region of chromosome 10 containing the JMJD1C positive control gene (fig 2A and B), and in 2 genomic regions surrounding HOX cluster on chromosome 7 and the SENP6 gene on chromosome 6 (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against MLL1 (Cat. No. C15310264). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:15,100.</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 700 kb region of chromosome 10 containing the JMJD1C positive control gene (fig 2A and B), and in 2 genomic regions surrounding HOX cluster on chromosome 7 and the SENP6 gene on chromosome 6 (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against MLL1 (Cat. No. C15310264). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:15,100.</small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed on MV4-11 cells using the Diagenode antibody against MLL1 (Cat. No. C15310264). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 700 kb region of chromosome 10 containing the JMJD1C positive control gene (fig 2A and B), and in 2 genomic regions surrounding HOX cluster on chromosome 7 and the SENP6 gene on chromosome 6 (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against MLL1 (Cat. No. C15310264). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:15,100.</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against MLL1 (Cat. No. C15310264). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:15,100.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed on MV4-11 cells using the Diagenode antibody against MLL1 (Cat. No. C15310264). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 700 kb region of chromosome 10 containing the JMJD1C positive control gene (fig 2A and B), and in 2 genomic regions surrounding HOX cluster on chromosome 7 and the SENP6 gene on chromosome 6 (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against MLL1 (Cat. No. C15310264). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:15,100.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed on MV4-11 cells using the Diagenode antibody against MLL1 (Cat. No. C15310264). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 700 kb region of chromosome 10 containing the JMJD1C positive control gene (fig 2A and B), and in 2 genomic regions surrounding HOX cluster on chromosome 7 and the SENP6 gene on chromosome 6 (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against MLL1 (Cat. No. C15310264). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:15,100.</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 700 kb region of chromosome 10 containing the JMJD1C positive control gene (fig 2A and B), and in 2 genomic regions surrounding HOX cluster on chromosome 7 and the SENP6 gene on chromosome 6 (fig 2C and D).</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed on MV4-11 cells using the Diagenode antibody against MLL1 (Cat. No. C15310264). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 700 kb region of chromosome 10 containing the JMJD1C positive control gene (fig 2A and B), and in 2 genomic regions surrounding HOX cluster on chromosome 7 and the SENP6 gene on chromosome 6 (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against MLL1 (Cat. No. C15310264). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:15,100.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed on MV4-11 cells using the Diagenode antibody against MLL1 (Cat. No. C15310264). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15310264-chipseq-b.png" alt="MLL1 Antibody for ChIP-seq" caption="false" width="700" height="149" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310264-chipseq-c.png" alt="MLL1 Antibody for ChIP-seq assay" caption="false" width="700" height="140" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310264-chipseq-D.png" alt="MLL1 Antibody validated in ChIP-seq" caption="false" width="700" height="108" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 700 kb region of chromosome 10 containing the JMJD1C positive control gene (fig 2A and B), and in 2 genomic regions surrounding HOX cluster on chromosome 7 and the SENP6 gene on chromosome 6 (fig 2C and D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15310264-elisa.png" alt="MLL1 Antibody ELISA validation" caption="false" width="447" height="337" /></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against MLL1 (Cat. No. C15310264). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:15,100.</small></p>
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<td>Fig 1, 2</td>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed on MV4-11 cells using the Diagenode antibody against MLL1 (Cat. No. C15310264). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MLL1</strong><br />ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 700 kb region of chromosome 10 containing the JMJD1C positive control gene (fig 2A and B), and in 2 genomic regions surrounding HOX cluster on chromosome 7 and the SENP6 gene on chromosome 6 (fig 2C and D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15310264-elisa.png" alt="MLL1 Antibody ELISA validation" caption="false" width="447" height="337" /></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against MLL1 (Cat. No. C15310264). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:15,100.</small></p>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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