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MYH11 polyclonal antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

Antibody portal

Antibodies

Catalog Number

Format

Price

C15310254

100 μl

$380.00
Bulk order

Other format

C15310254-20

20 μl

Alternative names: SMMHC, AAT4, FAA4, SMHC

Polyclonal antibody raised in rabbit against human MYH11 (Myosin, Heavy Chain 11) using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.

Lot	A1379-001
Concentration	not determined
Species reactivity	Human
Type	Polyclonal
Purity	Whole antiserum
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP/ChIP-seq ^*	5 μl/IP	Fig 1, 2
ELISA	1:200	Fig 3
Western Blotting	1:1,000	Fig 4

Validation Data

Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11
ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.

A.

B.

C.

D.

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11
ChIP was performed as described above. The IP’d DNA from 6 ChIP’s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.

Figure 3. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.

Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11
Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.
Target Description
MYH11 (UniProtKB/Swiss-Prot entry P35749) is a smooth muscle myosin belonging to the myosin heavy chain family which function as major contractile proteins. MYH11 is involved in a pericentric inversion of chromosome 16 (inv(16)(p13q22)) which produces a chimeric transcript consisting of the N terminus of CBFb and the C-terminal portion MYH11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype.

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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>5 &mu;l/IP</td>
<td>Fig 1, 2</td>
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<td>ELISA</td>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small><strong>A.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-A.png" alt="MYH11 Antibody ChIP-seq Grade" caption="false" width="447" height="60" /><br /><small><strong>B.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-B.png" alt="MYH11 Antibody for ChIP-seq " caption="false" width="447" height="57" /><br /><small><strong>C. </strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-C.png" alt="MYH11 Antibody for ChIP-seq assay" caption="false" width="447" height="57" /><br /><small><strong>D.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-D.png" alt="MYH11 Antibody validated in ChIP-seq" caption="false" width="447" height="56" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ELISA.png" alt="MYH11 Antibody ELISA Validation" caption="false" width="400" height="303" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_WB.png" height="173" width="206" alt="MYH11 Antibody validated in Western Blot" caption="false" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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<p><span>Polyclonal antibody raised in rabbit against human MYH11 (Myosin, Heavy Chain 11) using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.</span></p>',
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small><strong>A.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-A.png" alt="MYH11 Antibody ChIP-seq Grade" caption="false" width="447" height="60" /><br /><small><strong>B.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-B.png" alt="MYH11 Antibody for ChIP-seq " caption="false" width="447" height="57" /><br /><small><strong>C. </strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-C.png" alt="MYH11 Antibody for ChIP-seq assay" caption="false" width="447" height="57" /><br /><small><strong>D.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-D.png" alt="MYH11 Antibody validated in ChIP-seq" caption="false" width="447" height="56" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ELISA.png" alt="MYH11 Antibody ELISA Validation" caption="false" width="400" height="303" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_WB.png" height="173" width="206" alt="MYH11 Antibody validated in Western Blot" caption="false" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
</div>
</div>',
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		'meta_title' => 'MYH11 polyclonal antibody - Classic',
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		'meta_description' => 'MYH11 polyclonal antibody - Classic',
		'modified' => '2021-12-23 12:24:21',
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		'id' => '193',
		'name' => 'MYH11 polyclonal antibody',
		'description' => 'MYH11 (UniProtKB/Swiss-Prot entry P35749) is a smooth muscle myosin belonging to the myosin heavy chain family which function as major contractile proteins. MYH11 is involved in a pericentric inversion of chromosome 16 (inv(16)(p13q22)) which produces a chimeric transcript consisting of the N terminus of CBFb and the C-terminal portion MYH11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A1379-001',
		'concentration' => 'not determined',
		'reactivity' => 'Human',
		'type' => 'Polyclonal',
		'purity' => 'Whole antiserum',
		'classification' => 'Classic',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>5 &mu;l/IP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:200</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
</tr>
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<p></p>',
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			'name' => 'MYH11 Antibody',
			'description' => '<p><span>Alternative names: <strong>SMMHC</strong>, <strong>AAT4</strong>, <strong>FAA4</strong>, <strong>SMHC</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human<strong> MYH11 (Myosin, Heavy Chain 11)</strong> using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.</span></p>',
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<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIP.png" alt="MYH11 Antibody ChIP Grade" caption="false" width="400" height="303" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-A.png" alt="MYH11 Antibody ChIP-seq Grade" caption="false" width="447" height="60" /><br /><small><strong>B.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-B.png" alt="MYH11 Antibody for ChIP-seq " caption="false" width="447" height="57" /><br /><small><strong>C. </strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-C.png" alt="MYH11 Antibody for ChIP-seq assay" caption="false" width="447" height="57" /><br /><small><strong>D.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-D.png" alt="MYH11 Antibody validated in ChIP-seq" caption="false" width="447" height="56" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ELISA.png" alt="MYH11 Antibody ELISA Validation" caption="false" width="400" height="303" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_WB.png" height="173" width="206" alt="MYH11 Antibody validated in Western Blot" caption="false" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
</div>
</div>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
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</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
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			'description' => '<p><span>Alternative names: <strong>SMMHC</strong>, <strong>AAT4</strong>, <strong>FAA4</strong>, <strong>SMHC</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human MYH11 (Myosin, Heavy Chain 11) using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.</span></p>',
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIP.png" alt="MYH11 Antibody ChIP Grade" caption="false" width="400" height="303" /></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small><strong>A.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-A.png" alt="MYH11 Antibody ChIP-seq Grade" caption="false" width="447" height="60" /><br /><small><strong>B.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-B.png" alt="MYH11 Antibody for ChIP-seq " caption="false" width="447" height="57" /><br /><small><strong>C. </strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-C.png" alt="MYH11 Antibody for ChIP-seq assay" caption="false" width="447" height="57" /><br /><small><strong>D.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-D.png" alt="MYH11 Antibody validated in ChIP-seq" caption="false" width="447" height="56" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_WB.png" height="173" width="206" alt="MYH11 Antibody validated in Western Blot" caption="false" /></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<td>5 &mu;l/IP</td>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small><strong>A.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-A.png" alt="MYH11 Antibody ChIP-seq Grade" caption="false" width="447" height="60" /><br /><small><strong>B.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-B.png" alt="MYH11 Antibody for ChIP-seq " caption="false" width="447" height="57" /><br /><small><strong>C. </strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-C.png" alt="MYH11 Antibody for ChIP-seq assay" caption="false" width="447" height="57" /><br /><small><strong>D.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-D.png" alt="MYH11 Antibody validated in ChIP-seq" caption="false" width="447" height="56" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<p class="text-center"></p>
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<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<div class="small-6 columns">
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<li>Batch-specific data is available on the website</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<p><span>Polyclonal antibody raised in rabbit against human MYH11 (Myosin, Heavy Chain 11) using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.</span></p>',
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<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIP.png" alt="MYH11 Antibody ChIP Grade" caption="false" width="400" height="303" /></p>
<p class="text-center"></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small><strong>A.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-A.png" alt="MYH11 Antibody ChIP-seq Grade" caption="false" width="447" height="60" /><br /><small><strong>B.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-B.png" alt="MYH11 Antibody for ChIP-seq " caption="false" width="447" height="57" /><br /><small><strong>C. </strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-C.png" alt="MYH11 Antibody for ChIP-seq assay" caption="false" width="447" height="57" /><br /><small><strong>D.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-D.png" alt="MYH11 Antibody validated in ChIP-seq" caption="false" width="447" height="56" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ELISA.png" alt="MYH11 Antibody ELISA Validation" caption="false" width="400" height="303" /></p>
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<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<th>Suggested dilution</th>
<th>References</th>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>5 &mu;l/IP</td>
<td>Fig 1, 2</td>
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<tr>
<td>ELISA</td>
<td>1:200</td>
<td>Fig 3</td>
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<tr>
<td>Western Blotting</td>
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<div class="small-6 columns">
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<p class="text-center"></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-A.png" alt="MYH11 Antibody ChIP-seq Grade" caption="false" width="447" height="60" /><br /><small><strong>B.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-B.png" alt="MYH11 Antibody for ChIP-seq " caption="false" width="447" height="57" /><br /><small><strong>C. </strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-C.png" alt="MYH11 Antibody for ChIP-seq assay" caption="false" width="447" height="57" /><br /><small><strong>D.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-D.png" alt="MYH11 Antibody validated in ChIP-seq" caption="false" width="447" height="56" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ELISA.png" alt="MYH11 Antibody ELISA Validation" caption="false" width="400" height="303" /></p>
<p class="text-center"></p>
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<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_WB.png" height="173" width="206" alt="MYH11 Antibody validated in Western Blot" caption="false" /></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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		'ProductsGroup' => array(
			'id' => '360',
			'product_id' => '3141',
			'group_id' => '320'
		)
	)
)
$pro = array(
	'id' => '3141',
	'antibody_id' => '615',
	'name' => 'MYH11 Antibody (sample size)',
	'description' => '',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '20 μl',
	'catalog_number' => 'C15310254-20',
	'old_catalog_number' => '',
	'sf_code' => 'C15310254-361',
	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '105',
	'price_USD' => '115',
	'price_GBP' => '100',
	'price_JPY' => '16450',
	'price_CNY' => '',
	'price_AUD' => '288',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '',
	'slug' => 'myh11-polyclonal-antibody-classic-20',
	'meta_title' => '',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2021-12-23 12:24:31',
	'created' => '2020-11-25 15:54:15',
	'ProductsGroup' => array(
		'id' => '360',
		'product_id' => '3141',
		'group_id' => '320'
	)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
	'id' => '3141',
	'antibody_id' => '615',
	'name' => 'MYH11 Antibody (sample size)',
	'description' => '',
	'label1' => '',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '20 μl',
	'catalog_number' => 'C15310254-20',
	'old_catalog_number' => '',
	'sf_code' => 'C15310254-361',
	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '105',
	'price_USD' => '115',
	'price_GBP' => '100',
	'price_JPY' => '16450',
	'price_CNY' => '',
	'price_AUD' => '288',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => '',
	'slug' => 'myh11-polyclonal-antibody-classic-20',
	'meta_title' => '',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2021-12-23 12:24:31',
	'created' => '2020-11-25 15:54:15',
	'ProductsGroup' => array(
		'id' => '360',
		'product_id' => '3141',
		'group_id' => '320'
	)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '43',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'ChIP-qPCR (ab)',
	'description' => '',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'chip-qpcr-antibodies',
	'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
	'meta_title' => 'ChIP Quantitative PCR  Antibodies (ChIP-qPCR) | Diagenode',
	'modified' => '2016-01-20 11:30:24',
	'created' => '2015-10-20 11:45:36',
	'ProductsApplication' => array(
		'id' => '4110',
		'product_id' => '2165',
		'application_id' => '43'
	)
)
$slugs = array(
	(int) 0 => 'chip-qpcr-antibodies'
)
$applications = array(
	'id' => '43',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'ChIP-qPCR (ab)',
	'description' => '',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'chip-qpcr-antibodies',
	'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
	'meta_title' => 'ChIP Quantitative PCR  Antibodies (ChIP-qPCR) | Diagenode',
	'modified' => '2016-01-20 11:30:24',
	'created' => '2015-10-20 11:45:36',
	'locale' => 'jpn'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
	'id' => '38',
	'name' => 'Epigenetic Antibodies Brochure',
	'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
	'image_id' => null,
	'type' => 'Brochure',
	'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
	'slug' => 'epigenetic-antibodies-brochure',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2016-06-15 11:24:06',
	'created' => '2015-07-03 16:05:27',
	'ProductsDocument' => array(
		'id' => '1430',
		'product_id' => '2165',
		'document_id' => '38'
	)
)
$sds = array(
	'id' => '2796',
	'name' => 'MYH11 Antibody SDS ES es',
	'language' => 'es',
	'url' => 'files/SDS/MYH11/SDS-C15310254-MYH11_Antibody-ES-es-GHS_1_0.pdf',
	'countries' => 'ES',
	'modified' => '2022-10-06 14:29:41',
	'created' => '2022-10-06 14:29:41',
	'ProductsSafetySheet' => array(
		'id' => '4631',
		'product_id' => '2165',
		'safety_sheet_id' => '2796'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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Datasheet MYH11 C15310254 DATASHEET Datasheet description	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

MYH11 Antibody SDS GB en	Download
MYH11 Antibody SDS US en	Download
MYH11 Antibody SDS DE de	Download
MYH11 Antibody SDS JP ja	Download
MYH11 Antibody SDS BE nl	Download
MYH11 Antibody SDS BE fr	Download
MYH11 Antibody SDS FR fr	Download
MYH11 Antibody SDS ES es	Download

MYH11 polyclonal antibody

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