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MYH11 Antibody - ChIP-seq Grade (sample size)

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Antibodies

Catalog Number

Format

Price

C15310254-20

20 μl

$115.00
Bulk order

Other format

C15310254

100 μl

Alternative names: SMMHC, AAT4, FAA4, SMHC

Polyclonal antibody raised in rabbit against human MYH11 (Myosin, Heavy Chain 11) using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.

Lot	A1379-001
Concentration	not determined
Species reactivity	Human
Type	Polyclonal
Purity	Whole antiserum
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP/ChIP-seq ^*	5 μl/IP	Fig 1, 2
ELISA	1:200	Fig 3
Western Blotting	1:1,000	Fig 4

Validation Data

Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11
ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.

A.

B.

C.

D.

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11
ChIP was performed as described above. The IP’d DNA from 6 ChIP’s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.

Figure 3. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.

Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11
Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.
Target Description
MYH11 (UniProtKB/Swiss-Prot entry P35749) is a smooth muscle myosin belonging to the myosin heavy chain family which function as major contractile proteins. MYH11 is involved in a pericentric inversion of chromosome 16 (inv(16)(p13q22)) which produces a chimeric transcript consisting of the N terminus of CBFb and the C-terminal portion MYH11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype.

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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ELISA.png" alt="MYH11 Antibody ELISA Validation" caption="false" width="400" height="303" /></p>
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<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
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<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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		'id' => '2165',
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		'name' => 'MYH11 Antibody',
		'description' => '<p><span>Alternative names: <strong>SMMHC</strong>, <strong>AAT4</strong>, <strong>FAA4</strong>, <strong>SMHC</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human<strong> MYH11 (Myosin, Heavy Chain 11)</strong> using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.</span></p>',
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<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIP.png" alt="MYH11 Antibody ChIP Grade" caption="false" width="400" height="303" /></p>
<p class="text-center"></p>
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<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-A.png" alt="MYH11 Antibody ChIP-seq Grade" caption="false" width="447" height="60" /><br /><small><strong>B.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-B.png" alt="MYH11 Antibody for ChIP-seq " caption="false" width="447" height="57" /><br /><small><strong>C. </strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-C.png" alt="MYH11 Antibody for ChIP-seq assay" caption="false" width="447" height="57" /><br /><small><strong>D.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-D.png" alt="MYH11 Antibody validated in ChIP-seq" caption="false" width="447" height="56" /></p>
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<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ELISA.png" alt="MYH11 Antibody ELISA Validation" caption="false" width="400" height="303" /></p>
<p class="text-center"></p>
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<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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View::render() - CORE/Cake/View/View.php, line 473
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ReflectionMethod::invokeArgs() - [internal], line ??
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_WB.png" height="173" width="206" alt="MYH11 Antibody validated in Western Blot" caption="false" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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</div>',
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		'info2' => '<p>MYH11 (UniProtKB/Swiss-Prot entry P35749) is a smooth muscle myosin belonging to the myosin heavy chain family which function as major contractile proteins. MYH11 is involved in a pericentric inversion of chromosome 16 (inv(16)(p13q22)) which produces a chimeric transcript consisting of the N terminus of CBFb and the C-terminal portion MYH11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype.</p>',
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		'id' => '615',
		'name' => 'MYH11 Antibody - ChIP-seq Grade (sample size)',
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		'clonality' => '',
		'isotype' => '',
		'lot' => 'A1379-001',
		'concentration' => 'not determined',
		'reactivity' => 'Human',
		'type' => 'Polyclonal',
		'purity' => 'Whole antiserum',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
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<tbody>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>5 &mu;l/IP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:200</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
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<p></p>',
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			'name' => 'MYH11 Antibody',
			'description' => '<p><span>Alternative names: <strong>SMMHC</strong>, <strong>AAT4</strong>, <strong>FAA4</strong>, <strong>SMHC</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human<strong> MYH11 (Myosin, Heavy Chain 11)</strong> using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.</span></p>',
			'label1' => 'Validation Data',
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<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIP.png" alt="MYH11 Antibody ChIP Grade" caption="false" width="400" height="303" /></p>
<p class="text-center"></p>
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<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-A.png" alt="MYH11 Antibody ChIP-seq Grade" caption="false" width="447" height="60" /><br /><small><strong>B.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-B.png" alt="MYH11 Antibody for ChIP-seq " caption="false" width="447" height="57" /><br /><small><strong>C. </strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-C.png" alt="MYH11 Antibody for ChIP-seq assay" caption="false" width="447" height="57" /><br /><small><strong>D.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-D.png" alt="MYH11 Antibody validated in ChIP-seq" caption="false" width="447" height="56" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ELISA.png" alt="MYH11 Antibody ELISA Validation" caption="false" width="400" height="303" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
</div>
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<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_WB.png" height="173" width="206" alt="MYH11 Antibody validated in Western Blot" caption="false" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
</div>
</div>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p></p>
<p></p>
<p></p>
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<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
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<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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		'id' => '2165',
		'antibody_id' => '193',
		'name' => 'MYH11 Antibody',
		'description' => '<p><span>Alternative names: <strong>SMMHC</strong>, <strong>AAT4</strong>, <strong>FAA4</strong>, <strong>SMHC</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human<strong> MYH11 (Myosin, Heavy Chain 11)</strong> using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.</span></p>',
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<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIP.png" alt="MYH11 Antibody ChIP Grade" caption="false" width="400" height="303" /></p>
<p class="text-center"></p>
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<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-A.png" alt="MYH11 Antibody ChIP-seq Grade" caption="false" width="447" height="60" /><br /><small><strong>B.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-B.png" alt="MYH11 Antibody for ChIP-seq " caption="false" width="447" height="57" /><br /><small><strong>C. </strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-C.png" alt="MYH11 Antibody for ChIP-seq assay" caption="false" width="447" height="57" /><br /><small><strong>D.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-D.png" alt="MYH11 Antibody validated in ChIP-seq" caption="false" width="447" height="56" /></p>
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<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ELISA.png" alt="MYH11 Antibody ELISA Validation" caption="false" width="400" height="303" /></p>
<p class="text-center"></p>
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<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_WB.png" height="173" width="206" alt="MYH11 Antibody validated in Western Blot" caption="false" /></p>
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<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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</div>',
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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View::render() - CORE/Cake/View/View.php, line 473
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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		'id' => '615',
		'name' => 'MYH11 Antibody - ChIP-seq Grade (sample size)',
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		'isotype' => '',
		'lot' => 'A1379-001',
		'concentration' => 'not determined',
		'reactivity' => 'Human',
		'type' => 'Polyclonal',
		'purity' => 'Whole antiserum',
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		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>5 &mu;l/IP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:200</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 4</td>
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<p></p>',
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			'name' => 'MYH11 Antibody',
			'description' => '<p><span>Alternative names: <strong>SMMHC</strong>, <strong>AAT4</strong>, <strong>FAA4</strong>, <strong>SMHC</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human<strong> MYH11 (Myosin, Heavy Chain 11)</strong> using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.</span></p>',
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<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIP.png" alt="MYH11 Antibody ChIP Grade" caption="false" width="400" height="303" /></p>
<p class="text-center"></p>
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<div class="small-6 columns">
<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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</div>
<div class="row">
<div class="small-6 columns">
<p><small><strong>A.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-A.png" alt="MYH11 Antibody ChIP-seq Grade" caption="false" width="447" height="60" /><br /><small><strong>B.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-B.png" alt="MYH11 Antibody for ChIP-seq " caption="false" width="447" height="57" /><br /><small><strong>C. </strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-C.png" alt="MYH11 Antibody for ChIP-seq assay" caption="false" width="447" height="57" /><br /><small><strong>D.</strong></small><br /><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ChIPSeq-D.png" alt="MYH11 Antibody validated in ChIP-seq" caption="false" width="447" height="56" /></p>
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<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_ELISA.png" alt="MYH11 Antibody ELISA Validation" caption="false" width="400" height="303" /></p>
<p class="text-center"></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310254_WB.png" height="173" width="206" alt="MYH11 Antibody validated in Western Blot" caption="false" /></p>
</div>
<div class="small-6 columns">
<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
</div>
</div>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p></p>
<p></p>
<p></p>
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<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP assays were performed using ME-1 cells, the Diagenode antibody against MYH11 (Cat. No. C15310254) and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure 1 shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against MYH11</strong><br />ChIP was performed as described above. The IP&rsquo;d DNA from 6 ChIP&rsquo;s was pooled and subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal in 4 genomic regions surrounding the AXIN1, FUT7, BCL3 and RAD50 positive control genes.</small></p>
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<p><small> <strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human MYH11 (Cat. No. C15310254). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,900.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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<p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against MYH11</strong><br />Nuclear extracts of ME-1 cells were analysed by Western blot using the Diagenode antibodies against CBFb (Cat. No. C15310002, lane 1) and MYH11 (cat. No. C15310254, lane 2) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.</small></p>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
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Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
Datasheet MYH11 C15310254 DATASHEET Datasheet description	Download

MYH11 Antibody SDS GB en	Download
MYH11 Antibody SDS US en	Download
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MYH11 Antibody - ChIP-seq Grade (sample size)

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