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Other names: NCoR, TRAC1, PPP1R109
Polyclonal antibody raised in rabbit against human NCOR1 (Nuclear Receptor Corepressor 1), using a synthetic peptide containing a sequence from the central part of the protein1.
1Manufactured by Bethyl Laboratories, Inc., Texas, USA
| Lot | A301-145A3 |
|---|---|
| Concentration | 0.2 μg/μl |
| Species reactivity | Human: positive. Other species: not tested. |
| Type | Polyclonal |
| Purity | Affinity purified polyclonal antibody. |
| Host | Rabbit |
| Storage Conditions | Store at 4°C. |
| Storage Buffer | TBS containing 0.1% BSA and 0.09% azide. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP/ChIP-seq * | 2 μg/ChIP | Fig 1, 2 |
| Western Blotting | 1:5,000 | Fig 3 |
| IP | 6 μg per IP | Fig 4 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.
1Manufactured by Bethyl Laboratories, Inc., Texas, USA
Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1
ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
A.
B.
C.
D.
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1
ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).
Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1
Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1
Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.
NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. NCOR1 is part of a complex which may impede the access of basal transcription factors.by promoting histone deacetylation and the formation of repressive chromatin structures.
| ChIP-seq (ab) Read more |
| ChIP-qPCR (ab) Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IP Immunoprecipitation Read more |
| NCOR1 polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against human NCOR1 (Nuclear Receptor Corepressor 1), using ... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: NCOR1 polyclonal antibody (Diagenode Cat# C15410341 Lot# A301-145A3). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'NCOR1 polyclonal antibody', 'product' => array( 'Product' => array( 'id' => '2920', 'antibody_id' => '515', 'name' => 'NCOR1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> NCoR, TRAC1, PPP1R109</p><p>Polyclonal antibody raised in rabbit against human NCOR1 (Nuclear Receptor Corepressor 1), using a synthetic peptide containing a sequence from the central part of the protein<sup>1</sup>.</p><p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-chip.jpg" alt="NCOR1 Antibody ChIP Grade" caption="false" width="447" height="338" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p> <p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p> <p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p> <p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-wb.jpg" alt="NCOR1 Antibody validated in Western Blot" caption="false" width="208" height="396" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-ip.jpg" alt="NCOR1 Antibody validated in Immunoprecipitation " caption="false" width="208" height="390" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). 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ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p> <p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p> <p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p> <p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-wb.jpg" alt="NCOR1 Antibody validated in Western Blot" caption="false" width="208" height="396" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-ip.jpg" alt="NCOR1 Antibody validated in Immunoprecipitation " caption="false" width="208" height="390" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). 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We recommend testing 1-5 µg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => 'TBS containing 0.1% BSA and 0.09% azide.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ncor1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-12-02 16:11:56', 'created' => '2017-12-21 10:20:14', 'select_label' => '515 - NCOR1 polyclonal antibody (A301-145A3 - 0.2 μg/μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '342', 'name' => 'C15410341', 'product_id' => '2920', 'modified' => '2020-12-30 14:15:28', 'created' => '2020-12-30 14:15:28' ) ), 'Group' => array( 'Group' => array( 'id' => '342', 'name' => 'C15410341', 'product_id' => '2920', 'modified' => '2020-12-30 14:15:28', 'created' => '2020-12-30 14:15:28' ), 'Master' => array( 'id' => '2920', 'antibody_id' => '515', 'name' => 'NCOR1 Antibody', 'description' => '<p><strong>Other names:</strong> NCoR, TRAC1, PPP1R109</p> <p>Polyclonal antibody raised in rabbit against human <strong>NCOR1 (Nuclear Receptor Corepressor 1)</strong>, using a synthetic peptide containing a sequence from the central part of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-chip.jpg" alt="NCOR1 Antibody ChIP Grade" caption="false" width="447" height="338" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p> <p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p> <p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p> <p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-wb.jpg" alt="NCOR1 Antibody validated in Western Blot" caption="false" width="208" height="396" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-ip.jpg" alt="NCOR1 Antibody validated in Immunoprecipitation " caption="false" width="208" height="390" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. 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Batch-specific data available on the website. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '18', 'position' => '20', 'parent_id' => '4', 'name' => 'Chromatin associated proteins', 'description' => '<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '985', 'name' => 'NCOR1 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human NCOR1 (Nuclear Receptor Corepressor 1), using a synthetic peptide containing a sequence from the central part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_NCOR_C15410341.pdf', 'slug' => 'ncor1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-03 11:17:34', 'created' => '2018-01-03 11:17:34', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4661', 'name' => 'Estrone, the major postmenopausal estrogen, binds ERa to induceSNAI2, epithelial-to-mesenchymal transition, and ER+ breast cancermetastasis.', 'authors' => 'Qureshi R. et al.', 'description' => '<p>Recent work showed that the dominant post-menopausal estrogen, estrone, cooperates with nuclear factor κB (NF-κB) to stimulate inflammation, while pre-menopausal 17β-estradiol opposes NF-κB. Here, we show that post-menopausal estrone, but not 17β-estradiol, activates epithelial-to-mesenchymal transition (EMT) genes to stimulate breast cancer metastasis. HSD17B14, which converts 17β-estradiol to estrone, is higher in cancer than normal breast tissue and in metastatic than primary cancers and associates with earlier metastasis. Treatment with estrone, but not 17β-estradiol, and HSD17B14 overexpression both stimulate an EMT, matrigel invasion, and lung, bone, and liver metastasis in estrogen-receptor-positive (ER+) breast cancer models, while HSD17B14 knockdown reverses the EMT. Estrone:ERα recruits CBP/p300 to the SNAI2 promoter to induce SNAI2 and stimulate an EMT, while 17β-estradiol:ERα recruits co-repressors HDAC1 and NCOR1 to this site. Present work reveals novel differences in gene regulation by these estrogens and the importance of estrone to ER+ breast cancer progression. Upon loss of 17β-estradiol at menopause, estrone-liganded ERα would promote ER+ breast cancer invasion and metastasis.</p>', 'date' => '2022-11-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36384125', 'doi' => '10.1016/j.celrep.2022.111672', 'modified' => '2023-03-07 08:33:02', 'created' => '2023-02-21 09:59:46', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '2472', 'name' => 'NCOR1 Antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/NCOR1/SDS-C15410341-NCOR1_Antibody-US-en-GHS_1_0.pdf', 'countries' => 'US', 'modified' => '2022-08-30 11:14:37', 'created' => '2022-08-30 11:14:37', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2470', 'name' => 'NCOR1 Antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/NCOR1/SDS-C15410341-NCOR1_Antibody-GB-en-GHS_1_0.pdf', 'countries' => 'GB', 'modified' => '2022-08-30 11:14:37', 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'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'locale' => 'jpn' ) $description = '<p>Immunoprecipitation</p>' $name = 'IP' $document = array( 'id' => '985', 'name' => 'NCOR1 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human NCOR1 (Nuclear Receptor Corepressor 1), using a synthetic peptide containing a sequence from the central part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_NCOR_C15410341.pdf', 'slug' => 'ncor1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-03 11:17:34', 'created' => '2018-01-03 11:17:34', 'ProductsDocument' => array( 'id' => '2494', 'product_id' => '2920', 'document_id' => '985' ) ) $sds = array( 'id' => '2466', 'name' => 'NCOR1 Antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/NCOR1/SDS-C15410341-NCOR1_Antibody-BE-nl-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-30 11:14:37', 'created' => '2022-08-30 11:14:37', 'ProductsSafetySheet' => array( 'id' => '4187', 'product_id' => '2920', 'safety_sheet_id' => '2466' ) ) $publication = array( 'id' => '4661', 'name' => 'Estrone, the major postmenopausal estrogen, binds ERa to induceSNAI2, epithelial-to-mesenchymal transition, and ER+ breast cancermetastasis.', 'authors' => 'Qureshi R. et al.', 'description' => '<p>Recent work showed that the dominant post-menopausal estrogen, estrone, cooperates with nuclear factor κB (NF-κB) to stimulate inflammation, while pre-menopausal 17β-estradiol opposes NF-κB. Here, we show that post-menopausal estrone, but not 17β-estradiol, activates epithelial-to-mesenchymal transition (EMT) genes to stimulate breast cancer metastasis. HSD17B14, which converts 17β-estradiol to estrone, is higher in cancer than normal breast tissue and in metastatic than primary cancers and associates with earlier metastasis. Treatment with estrone, but not 17β-estradiol, and HSD17B14 overexpression both stimulate an EMT, matrigel invasion, and lung, bone, and liver metastasis in estrogen-receptor-positive (ER+) breast cancer models, while HSD17B14 knockdown reverses the EMT. Estrone:ERα recruits CBP/p300 to the SNAI2 promoter to induce SNAI2 and stimulate an EMT, while 17β-estradiol:ERα recruits co-repressors HDAC1 and NCOR1 to this site. Present work reveals novel differences in gene regulation by these estrogens and the importance of estrone to ER+ breast cancer progression. Upon loss of 17β-estradiol at menopause, estrone-liganded ERα would promote ER+ breast cancer invasion and metastasis.</p>', 'date' => '2022-11-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36384125', 'doi' => '10.1016/j.celrep.2022.111672', 'modified' => '2023-03-07 08:33:02', 'created' => '2023-02-21 09:59:46', 'ProductsPublication' => array( 'id' => '6530', 'product_id' => '2920', 'publication_id' => '4661' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/36384125" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'NCOR1 polyclonal antibody', 'product' => array( 'Product' => array( 'id' => '2920', 'antibody_id' => '515', 'name' => 'NCOR1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> NCoR, TRAC1, PPP1R109</p><p>Polyclonal antibody raised in rabbit against human NCOR1 (Nuclear Receptor Corepressor 1), using a synthetic peptide containing a sequence from the central part of the protein<sup>1</sup>.</p><p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-chip.jpg" alt="NCOR1 Antibody ChIP Grade" caption="false" width="447" height="338" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p> <p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p> <p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p> <p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-wb.jpg" alt="NCOR1 Antibody validated in Western Blot" caption="false" width="208" height="396" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-ip.jpg" alt="NCOR1 Antibody validated in Immunoprecipitation " caption="false" width="208" height="390" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p> </div> </div>', 'label2' => '', 'info2' => '<p>NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. NCOR1 is part of a complex which may impede the access of basal transcription factors.by promoting histone deacetylation and the formation of repressive chromatin structures.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410341', 'old_catalog_number' => '', 'sf_code' => 'C15410341-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'December 2, 2020', 'slug' => 'ncor1-polyclonal-antibody-100-ul', 'meta_title' => 'NCOR1 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-14 13:27:19', 'created' => '2017-12-21 10:32:08', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '515', 'name' => 'NCOR1 polyclonal antibody', 'description' => 'NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. NCOR1 is part of a complex which may impede the access of basal transcription factors.by promoting histone deacetylation and the formation of repressive chromatin structures.', 'clonality' => '', 'isotype' => '', 'lot' => 'A301-145A3', 'concentration' => '0.2 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:5,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>6 μg per IP</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => 'TBS containing 0.1% BSA and 0.09% azide.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ncor1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-12-02 16:11:56', 'created' => '2017-12-21 10:20:14', 'select_label' => '515 - NCOR1 polyclonal antibody (A301-145A3 - 0.2 μg/μl - Human: positive. 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ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p> <p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p> <p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p> <p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-wb.jpg" alt="NCOR1 Antibody validated in Western Blot" caption="false" width="208" height="396" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-ip.jpg" alt="NCOR1 Antibody validated in Immunoprecipitation " caption="false" width="208" height="390" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. NCOR1 is part of a complex which may impede the access of basal transcription factors.by promoting histone deacetylation and the formation of repressive chromatin structures.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410341', 'old_catalog_number' => '', 'sf_code' => 'C15410341-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'December 2, 2020', 'slug' => 'ncor1-polyclonal-antibody-100-ul', 'meta_title' => 'NCOR1 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-14 13:27:19', 'created' => '2017-12-21 10:32:08', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '515', 'name' => 'NCOR1 polyclonal antibody', 'description' => 'NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. NCOR1 is part of a complex which may impede the access of basal transcription factors.by promoting histone deacetylation and the formation of repressive chromatin structures.', 'clonality' => '', 'isotype' => '', 'lot' => 'A301-145A3', 'concentration' => '0.2 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:5,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>6 μg per IP</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => 'TBS containing 0.1% BSA and 0.09% azide.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ncor1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-12-02 16:11:56', 'created' => '2017-12-21 10:20:14', 'select_label' => '515 - NCOR1 polyclonal antibody (A301-145A3 - 0.2 μg/μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '342', 'name' => 'C15410341', 'product_id' => '2920', 'modified' => '2020-12-30 14:15:28', 'created' => '2020-12-30 14:15:28' ) ), 'Group' => array( 'Group' => array( 'id' => '342', 'name' => 'C15410341', 'product_id' => '2920', 'modified' => '2020-12-30 14:15:28', 'created' => '2020-12-30 14:15:28' ), 'Master' => array( 'id' => '2920', 'antibody_id' => '515', 'name' => 'NCOR1 Antibody', 'description' => '<p><strong>Other names:</strong> NCoR, TRAC1, PPP1R109</p> <p>Polyclonal antibody raised in rabbit against human <strong>NCOR1 (Nuclear Receptor Corepressor 1)</strong>, using a synthetic peptide containing a sequence from the central part of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-chip.jpg" alt="NCOR1 Antibody ChIP Grade" caption="false" width="447" height="338" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p> <p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p> <p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p> <p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-wb.jpg" alt="NCOR1 Antibody validated in Western Blot" caption="false" width="208" height="396" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-ip.jpg" alt="NCOR1 Antibody validated in Immunoprecipitation " caption="false" width="208" height="390" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. 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Batch-specific data available on the website. Other names: NCoR, TRAC1, PPP1R109.', 'modified' => '2022-02-14 13:27:19', 'created' => '2017-12-21 10:32:08' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'NCOR1 polyclonal antibody', 'product' => array( 'Product' => array( 'id' => '2920', 'antibody_id' => '515', 'name' => 'NCOR1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> NCoR, TRAC1, PPP1R109</p><p>Polyclonal antibody raised in rabbit against human NCOR1 (Nuclear Receptor Corepressor 1), using a synthetic peptide containing a sequence from the central part of the protein<sup>1</sup>.</p><p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-chip.jpg" alt="NCOR1 Antibody ChIP Grade" caption="false" width="447" height="338" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p> <p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p> <p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p> <p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-wb.jpg" alt="NCOR1 Antibody validated in Western Blot" caption="false" width="208" height="396" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-ip.jpg" alt="NCOR1 Antibody validated in Immunoprecipitation " caption="false" width="208" height="390" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p> </div> </div>', 'label2' => '', 'info2' => '<p>NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. NCOR1 is part of a complex which may impede the access of basal transcription factors.by promoting histone deacetylation and the formation of repressive chromatin structures.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410341', 'old_catalog_number' => '', 'sf_code' => 'C15410341-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'December 2, 2020', 'slug' => 'ncor1-polyclonal-antibody-100-ul', 'meta_title' => 'NCOR1 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-14 13:27:19', 'created' => '2017-12-21 10:32:08', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '515', 'name' => 'NCOR1 polyclonal antibody', 'description' => 'NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. 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We recommend testing 1-5 µg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => 'TBS containing 0.1% BSA and 0.09% azide.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ncor1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-12-02 16:11:56', 'created' => '2017-12-21 10:20:14', 'select_label' => '515 - NCOR1 polyclonal antibody (A301-145A3 - 0.2 μg/μl - Human: positive. 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ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p> <p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p> <p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p> <p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-wb.jpg" alt="NCOR1 Antibody validated in Western Blot" caption="false" width="208" height="396" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-ip.jpg" alt="NCOR1 Antibody validated in Immunoprecipitation " caption="false" width="208" height="390" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. NCOR1 is part of a complex which may impede the access of basal transcription factors.by promoting histone deacetylation and the formation of repressive chromatin structures.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410341', 'old_catalog_number' => '', 'sf_code' => 'C15410341-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'December 2, 2020', 'slug' => 'ncor1-polyclonal-antibody-100-ul', 'meta_title' => 'NCOR1 polyclonal antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-14 13:27:19', 'created' => '2017-12-21 10:32:08', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '515', 'name' => 'NCOR1 polyclonal antibody', 'description' => 'NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. NCOR1 is part of a complex which may impede the access of basal transcription factors.by promoting histone deacetylation and the formation of repressive chromatin structures.', 'clonality' => '', 'isotype' => '', 'lot' => 'A301-145A3', 'concentration' => '0.2 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:5,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>6 μg per IP</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => 'TBS containing 0.1% BSA and 0.09% azide.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ncor1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-12-02 16:11:56', 'created' => '2017-12-21 10:20:14', 'select_label' => '515 - NCOR1 polyclonal antibody (A301-145A3 - 0.2 μg/μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '342', 'name' => 'C15410341', 'product_id' => '2920', 'modified' => '2020-12-30 14:15:28', 'created' => '2020-12-30 14:15:28' ) ), 'Group' => array( 'Group' => array( 'id' => '342', 'name' => 'C15410341', 'product_id' => '2920', 'modified' => '2020-12-30 14:15:28', 'created' => '2020-12-30 14:15:28' ), 'Master' => array( 'id' => '2920', 'antibody_id' => '515', 'name' => 'NCOR1 Antibody', 'description' => '<p><strong>Other names:</strong> NCoR, TRAC1, PPP1R109</p> <p>Polyclonal antibody raised in rabbit against human <strong>NCOR1 (Nuclear Receptor Corepressor 1)</strong>, using a synthetic peptide containing a sequence from the central part of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-chip.jpg" alt="NCOR1 Antibody ChIP Grade" caption="false" width="447" height="338" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p> <p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p> <p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p> <p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-wb.jpg" alt="NCOR1 Antibody validated in Western Blot" caption="false" width="208" height="396" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-ip.jpg" alt="NCOR1 Antibody validated in Immunoprecipitation " caption="false" width="208" height="390" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. 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Batch-specific data available on the website. 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'meta_description' => '', 'modified' => '2018-01-03 11:17:34', 'created' => '2018-01-03 11:17:34', 'ProductsDocument' => array( 'id' => '2494', 'product_id' => '2920', 'document_id' => '985' ) ) $sds = array( 'id' => '2466', 'name' => 'NCOR1 Antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/NCOR1/SDS-C15410341-NCOR1_Antibody-BE-nl-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-30 11:14:37', 'created' => '2022-08-30 11:14:37', 'ProductsSafetySheet' => array( 'id' => '4187', 'product_id' => '2920', 'safety_sheet_id' => '2466' ) ) $publication = array( 'id' => '4661', 'name' => 'Estrone, the major postmenopausal estrogen, binds ERa to induceSNAI2, epithelial-to-mesenchymal transition, and ER+ breast cancermetastasis.', 'authors' => 'Qureshi R. et al.', 'description' => '<p>Recent work showed that the dominant post-menopausal estrogen, estrone, cooperates with nuclear factor κB (NF-κB) to stimulate inflammation, while pre-menopausal 17β-estradiol opposes NF-κB. Here, we show that post-menopausal estrone, but not 17β-estradiol, activates epithelial-to-mesenchymal transition (EMT) genes to stimulate breast cancer metastasis. HSD17B14, which converts 17β-estradiol to estrone, is higher in cancer than normal breast tissue and in metastatic than primary cancers and associates with earlier metastasis. Treatment with estrone, but not 17β-estradiol, and HSD17B14 overexpression both stimulate an EMT, matrigel invasion, and lung, bone, and liver metastasis in estrogen-receptor-positive (ER+) breast cancer models, while HSD17B14 knockdown reverses the EMT. Estrone:ERα recruits CBP/p300 to the SNAI2 promoter to induce SNAI2 and stimulate an EMT, while 17β-estradiol:ERα recruits co-repressors HDAC1 and NCOR1 to this site. Present work reveals novel differences in gene regulation by these estrogens and the importance of estrone to ER+ breast cancer progression. Upon loss of 17β-estradiol at menopause, estrone-liganded ERα would promote ER+ breast cancer invasion and metastasis.</p>', 'date' => '2022-11-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36384125', 'doi' => '10.1016/j.celrep.2022.111672', 'modified' => '2023-03-07 08:33:02', 'created' => '2023-02-21 09:59:46', 'ProductsPublication' => array( 'id' => '6530', 'product_id' => '2920', 'publication_id' => '4661' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/36384125" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => 'NCOR1 polyclonal antibody', 'product' => array( 'Product' => array( 'id' => '2920', 'antibody_id' => '515', 'name' => 'NCOR1 polyclonal antibody', 'description' => '<p><strong>Other names:</strong> NCoR, TRAC1, PPP1R109</p><p>Polyclonal antibody raised in rabbit against human NCOR1 (Nuclear Receptor Corepressor 1), using a synthetic peptide containing a sequence from the central part of the protein<sup>1</sup>.</p><p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-chip.jpg" alt="NCOR1 Antibody ChIP Grade" caption="false" width="447" height="338" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p> <p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p> <p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p> <p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-wb.jpg" alt="NCOR1 Antibody validated in Western Blot" caption="false" width="208" height="396" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-ip.jpg" alt="NCOR1 Antibody validated in Immunoprecipitation " caption="false" width="208" height="390" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p> </div> </div>', 'label2' => '', 'info2' => '<p>NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. 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ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p> <p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p> <p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p> <p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-wb.jpg" alt="NCOR1 Antibody validated in Western Blot" caption="false" width="208" height="396" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-ip.jpg" alt="NCOR1 Antibody validated in Immunoprecipitation " caption="false" width="208" height="390" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. 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NCOR1 is part of a complex which may impede the access of basal transcription factors.by promoting histone deacetylation and the formation of repressive chromatin structures.', 'clonality' => '', 'isotype' => '', 'lot' => 'A301-145A3', 'concentration' => '0.2 μg/μl', 'reactivity' => 'Human: positive. Other species: not tested.', 'type' => 'Polyclonal', 'purity' => 'Affinity purified polyclonal antibody.', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:5,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td>6 μg per IP</td> <td>Fig 4</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => 'TBS containing 0.1% BSA and 0.09% azide.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'ncor1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-12-02 16:11:56', 'created' => '2017-12-21 10:20:14', 'select_label' => '515 - NCOR1 polyclonal antibody (A301-145A3 - 0.2 μg/μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '342', 'name' => 'C15410341', 'product_id' => '2920', 'modified' => '2020-12-30 14:15:28', 'created' => '2020-12-30 14:15:28' ) ), 'Group' => array( 'Group' => array( 'id' => '342', 'name' => 'C15410341', 'product_id' => '2920', 'modified' => '2020-12-30 14:15:28', 'created' => '2020-12-30 14:15:28' ), 'Master' => array( 'id' => '2920', 'antibody_id' => '515', 'name' => 'NCOR1 Antibody', 'description' => '<p><strong>Other names:</strong> NCoR, TRAC1, PPP1R109</p> <p>Polyclonal antibody raised in rabbit against human <strong>NCOR1 (Nuclear Receptor Corepressor 1)</strong>, using a synthetic peptide containing a sequence from the central part of the protein<sup>1</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-6 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-chip.jpg" alt="NCOR1 Antibody ChIP Grade" caption="false" width="447" height="338" /></p> </div> <div class="small-6 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p> <p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p> <p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p> <p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p> </div> </div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p> </div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-wb.jpg" alt="NCOR1 Antibody validated in Western Blot" caption="false" width="208" height="396" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-ip.jpg" alt="NCOR1 Antibody validated in Immunoprecipitation " caption="false" width="208" height="390" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. NCOR1 is part of a complex which may impede the access of basal transcription factors.by promoting histone deacetylation and the formation of repressive chromatin structures.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410341', 'old_catalog_number' => '', 'sf_code' => 'C15410341-D001-001161', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '0', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'December 2, 2020', 'slug' => 'ncor1-polyclonal-antibody-100-ul', 'meta_title' => 'NCOR1 Antibody - ChIP-seq Grade (C15410341) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'NCOR1 (Nuclear Receptor Corepressor 1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website. Other names: NCoR, TRAC1, PPP1R109.', 'modified' => '2022-02-14 13:27:19', 'created' => '2017-12-21 10:32:08' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '18', 'position' => '20', 'parent_id' => '4', 'name' => 'Chromatin associated proteins', 'description' => '<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '985', 'name' => 'NCOR1 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human NCOR1 (Nuclear Receptor Corepressor 1), using a synthetic peptide containing a sequence from the central part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_NCOR_C15410341.pdf', 'slug' => 'ncor1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-03 11:17:34', 'created' => '2018-01-03 11:17:34', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1783', 'name' => 'product/antibodies/chipseq-grade-ab-icon.png', 'alt' => 'ChIP-seq Grade', 'modified' => '2020-11-27 07:04:40', 'created' => '2018-03-15 15:54:09', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4661', 'name' => 'Estrone, the major postmenopausal estrogen, binds ERa to induceSNAI2, epithelial-to-mesenchymal transition, and ER+ breast cancermetastasis.', 'authors' => 'Qureshi R. et al.', 'description' => '<p>Recent work showed that the dominant post-menopausal estrogen, estrone, cooperates with nuclear factor κB (NF-κB) to stimulate inflammation, while pre-menopausal 17β-estradiol opposes NF-κB. Here, we show that post-menopausal estrone, but not 17β-estradiol, activates epithelial-to-mesenchymal transition (EMT) genes to stimulate breast cancer metastasis. HSD17B14, which converts 17β-estradiol to estrone, is higher in cancer than normal breast tissue and in metastatic than primary cancers and associates with earlier metastasis. Treatment with estrone, but not 17β-estradiol, and HSD17B14 overexpression both stimulate an EMT, matrigel invasion, and lung, bone, and liver metastasis in estrogen-receptor-positive (ER+) breast cancer models, while HSD17B14 knockdown reverses the EMT. Estrone:ERα recruits CBP/p300 to the SNAI2 promoter to induce SNAI2 and stimulate an EMT, while 17β-estradiol:ERα recruits co-repressors HDAC1 and NCOR1 to this site. Present work reveals novel differences in gene regulation by these estrogens and the importance of estrone to ER+ breast cancer progression. Upon loss of 17β-estradiol at menopause, estrone-liganded ERα would promote ER+ breast cancer invasion and metastasis.</p>', 'date' => '2022-11-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36384125', 'doi' => '10.1016/j.celrep.2022.111672', 'modified' => '2023-03-07 08:33:02', 'created' => '2023-02-21 09:59:46', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '2472', 'name' => 'NCOR1 Antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/NCOR1/SDS-C15410341-NCOR1_Antibody-US-en-GHS_1_0.pdf', 'countries' => 'US', 'modified' => '2022-08-30 11:14:37', 'created' => '2022-08-30 11:14:37', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2470', 'name' => 'NCOR1 Antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/NCOR1/SDS-C15410341-NCOR1_Antibody-GB-en-GHS_1_0.pdf', 'countries' => 'GB', 'modified' => '2022-08-30 11:14:37', 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(sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410341-20', 'old_catalog_number' => '', 'sf_code' => 'C15410341-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'ncor1-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 12:24:12', 'created' => '2020-12-30 14:14:44', 'ProductsGroup' => array( 'id' => '385', 'product_id' => '3179', 'group_id' => '342' ) ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( 'id' => '4751', 'product_id' => '2920', 'application_id' => '30' ) ) $slugs = array( (int) 0 => 'immunoprecipitation' ) $applications = array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'locale' => 'jpn' ) $description = '<p>Immunoprecipitation</p>' $name = 'IP' $document = array( 'id' => '985', 'name' => 'NCOR1 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human NCOR1 (Nuclear Receptor Corepressor 1), using a synthetic peptide containing a sequence from the central part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_NCOR_C15410341.pdf', 'slug' => 'ncor1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-03 11:17:34', 'created' => '2018-01-03 11:17:34', 'ProductsDocument' => array( 'id' => '2494', 'product_id' => '2920', 'document_id' => '985' ) ) $sds = array( 'id' => '2466', 'name' => 'NCOR1 Antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/NCOR1/SDS-C15410341-NCOR1_Antibody-BE-nl-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-30 11:14:37', 'created' => '2022-08-30 11:14:37', 'ProductsSafetySheet' => array( 'id' => '4187', 'product_id' => '2920', 'safety_sheet_id' => '2466' ) ) $publication = array( 'id' => '4661', 'name' => 'Estrone, the major postmenopausal estrogen, binds ERa to induceSNAI2, epithelial-to-mesenchymal transition, and ER+ breast cancermetastasis.', 'authors' => 'Qureshi R. et al.', 'description' => '<p>Recent work showed that the dominant post-menopausal estrogen, estrone, cooperates with nuclear factor κB (NF-κB) to stimulate inflammation, while pre-menopausal 17β-estradiol opposes NF-κB. Here, we show that post-menopausal estrone, but not 17β-estradiol, activates epithelial-to-mesenchymal transition (EMT) genes to stimulate breast cancer metastasis. HSD17B14, which converts 17β-estradiol to estrone, is higher in cancer than normal breast tissue and in metastatic than primary cancers and associates with earlier metastasis. Treatment with estrone, but not 17β-estradiol, and HSD17B14 overexpression both stimulate an EMT, matrigel invasion, and lung, bone, and liver metastasis in estrogen-receptor-positive (ER+) breast cancer models, while HSD17B14 knockdown reverses the EMT. Estrone:ERα recruits CBP/p300 to the SNAI2 promoter to induce SNAI2 and stimulate an EMT, while 17β-estradiol:ERα recruits co-repressors HDAC1 and NCOR1 to this site. Present work reveals novel differences in gene regulation by these estrogens and the importance of estrone to ER+ breast cancer progression. Upon loss of 17β-estradiol at menopause, estrone-liganded ERα would promote ER+ breast cancer invasion and metastasis.</p>', 'date' => '2022-11-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36384125', 'doi' => '10.1016/j.celrep.2022.111672', 'modified' => '2023-03-07 08:33:02', 'created' => '2023-02-21 09:59:46', 'ProductsPublication' => array( 'id' => '6530', 'product_id' => '2920', 'publication_id' => '4661' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/36384125" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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