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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p>
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<p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p>
<p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p>
<p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-wb.jpg" alt="NCOR1 Antibody validated in Western Blot" caption="false" width="208" height="396" /></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-ip.jpg" alt="NCOR1 Antibody validated in Immunoprecipitation " caption="false" width="208" height="390" /></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p>
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<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p>
<p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p>
<p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-wb.jpg" alt="NCOR1 Antibody validated in Western Blot" caption="false" width="208" height="396" /></p>
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<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-ip.jpg" alt="NCOR1 Antibody validated in Immunoprecipitation " caption="false" width="208" height="390" /></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 μg/ChIP</td>
<td>Fig 1, 2</td>
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<td>Western Blotting</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p>
<p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p>
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<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p>
<p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p>
<p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p>
<p>D.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-d.jpg" alt="NCOR1 Antibody validated in ChIP-seq " caption="false" width="700" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-wb.jpg" alt="NCOR1 Antibody validated in Western Blot" caption="false" width="208" height="396" /></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410341-ip.jpg" alt="NCOR1 Antibody validated in Immunoprecipitation " caption="false" width="208" height="390" /></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 μg/ChIP</td>
<td>Fig 1, 2</td>
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<td>Western Blotting</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p>A.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-a.jpg" alt="NCOR1 Antibody ChIP-seq Grade" caption="false" width="700" /></p>
<p>B.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-b.jpg" alt="NCOR1 Antibody for ChIP-seq " caption="false" width="700" /></p>
<p>C.<img src="https://www.diagenode.com/img/product/antibodies/C15410341-chipseq-c.jpg" alt="NCOR1 Antibody for ChIP-seq assay" caption="false" width="700" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1</strong><br />Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1</strong><br />Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/36384125" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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