Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase - unloaded',
'meta_title' => 'pA-Tn5 Transposase - unloaded',
'product' => array(
'Product' => array(
'id' => '3067',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '30 µl',
'catalog_number' => 'C01070002-30',
'old_catalog_number' => '',
'sf_code' => 'C01070002-143301',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1155',
'price_USD' => '1155',
'price_GBP' => '1010',
'price_JPY' => '/',
'price_CNY' => '0',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded-30-ul',
'meta_title' => 'pA-Tn5 Transposase - unloaded',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase - unloaded',
'modified' => '2024-08-29 09:37:23',
'created' => '2020-04-28 16:56:21',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/jp/p/pa-tn5-transposase-unloaded'
)
$language = 'jp'
$meta_keywords = ''
$meta_description = 'pA-Tn5 Transposase - unloaded'
$meta_title = 'pA-Tn5 Transposase - unloaded'
$product = array(
'Product' => array(
'id' => '3067',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '30 µl',
'catalog_number' => 'C01070002-30',
'old_catalog_number' => '',
'sf_code' => 'C01070002-143301',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1155',
'price_USD' => '1155',
'price_GBP' => '1010',
'price_JPY' => '/',
'price_CNY' => '0',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded-30-ul',
'meta_title' => 'pA-Tn5 Transposase - unloaded',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase - unloaded',
'modified' => '2024-08-29 09:37:23',
'created' => '2020-04-28 16:56:21',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '275',
'name' => 'C01070002',
'product_id' => '3065',
'modified' => '2020-04-28 16:56:42',
'created' => '2020-04-28 16:56:42'
),
'Master' => array(
'id' => '3065',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µl',
'catalog_number' => 'C01070002',
'old_catalog_number' => '',
'sf_code' => 'C01070002-19650',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '650',
'price_USD' => '650',
'price_GBP' => '575',
'price_JPY' => '/',
'price_CNY' => '/',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded',
'meta_title' => 'pA-Tn5 Transposase (unloaded) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:36:57',
'created' => '2020-04-28 16:21:20'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(
(int) 0 => array(
'id' => '142',
'position' => '4',
'parent_id' => '13',
'name' => 'CUT&Tag',
'description' => '<p><img alt=" iDeal CUT&Tag for Histones" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></p>
<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><strong>CUT&Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&Tag – our iDeal CUT&Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein – pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&Tag</a> proving their high performance in this assay.</p>
<br /> <a href="https://www.diagenode.com/files/application_notes/AN-iDealCUTandTag.pdf"><img src="https://www.diagenode.com/img/banners/cutandtag-appnote.png" /></a><br /><br /></div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); }
// ]]></script>
</center></div>
</div>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a>
<div id="v5" class="content">
<p>The iDeal CUT&Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode’s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode’s Primer Indexes for tagmented libraries.</p>
<img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div>
<h2>Products for CUT&Tag assay</h2>
<h3 class="diacol">Complete solutions</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&Tag kit for Histones</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">Fusion protein</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li>
</ul>
<h3 class="diacol">CUT&Tag grade antibodies</h3>
<ul class="nobullet">
<li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&Tag</a></li>
<li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li>
<li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&Tag</a></li>
</ul>
<h3 class="diacol">Positive & Negative CUT&Tag control</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">DNA purification</h3>
<p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p>
<h3 class="diacol">Sequencing indexes</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li>
</ul>
</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'cutandtag',
'cookies_tag_id' => null,
'meta_keywords' => '',
'meta_description' => 'Rapid chromatin profiling assay, optimized protocol, all reagents provided. Easy sample handling due to ConA magnetic beads. Highly specific CUT&Tag grade antibodies available. ',
'meta_title' => 'Diagenode provides advanced solutions for high performance CUT&Tag experiments',
'modified' => '2024-03-07 17:14:34',
'created' => '2020-04-28 15:07:36',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '1079',
'name' => 'pA-TN5 transposase (unloaded) datasheet',
'description' => '<p>pA-Tn5 transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For flexibility of use, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended assembly protocol prior to use in CUT&Tag or similar assays.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/cutandtag/C01070002-pa-tn5-transposase-unloaded.pdf',
'slug' => 'c01070002-pa-tn5-transposase-unloaded-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-04-28 16:45:02',
'created' => '2020-04-28 16:45:02',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1077',
'name' => 'Transposome Assembly Using Diagenode pA-Tn5 Transposase',
'description' => '<p>pA-Tn5 transposase (Cat. No. C01070002) is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For flexibility of use, the fusion protein is not pre-loaded with sequencing adapters. The fusion protein should be loaded with appropriate oligonucleotides prior to use. Oligonucleotides should contain 19-mer Tn5 mosaic ends (underlined) recognized by the transposase and the sequences (bold) allowing the PCR amplification with Illumina-compatible barcoded i7/i5 primers. These sequences have to be adapted to a particular experimental design and take into account the sequencing platform requirements.</p>',
'image_id' => '246',
'type' => 'Protocol',
'url' => 'files/products/cutandtag/assembly-protocol-pAtn5-20042020-rms.pdf',
'slug' => 'assembly-protocol-patn5-20042020-rms',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-04-28 16:32:44',
'created' => '2020-04-28 16:32:44',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4781',
'name' => 'Spatial epigenome-transcriptome co-profiling of mammalian tissues.',
'authors' => 'Zhang D. et al.',
'description' => '<p>Emerging spatial technologies, including spatial transcriptomics and spatial epigenomics, are becoming powerful tools for profiling of cellular states in the tissue context. However, current methods capture only one layer of omics information at a time, precluding the possibility of examining the mechanistic relationship across the central dogma of molecular biology. Here, we present two technologies for spatially resolved, genome-wide, joint profiling of the epigenome and transcriptome by cosequencing chromatin accessibility and gene expression, or histone modifications (H3K27me3, H3K27ac or H3K4me3) and gene expression on the same tissue section at near-single-cell resolution. These were applied to embryonic and juvenile mouse brain, as well as adult human brain, to map how epigenetic mechanisms control transcriptional phenotype and cell dynamics in tissue. Although highly concordant tissue features were identified by either spatial epigenome or spatial transcriptome we also observed distinct patterns, suggesting their differential roles in defining cell states. Linking epigenome to transcriptome pixel by pixel allows the uncovering of new insights in spatial epigenetic priming, differentiation and gene regulation within the tissue architecture. These technologies are of great interest in life science and biomedical research.</p>',
'date' => '2023-04-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36922587',
'doi' => '10.1038/s41586-023-05795-1',
'modified' => '2023-06-13 09:17:26',
'created' => '2023-05-05 12:34:24',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '4389',
'name' => 'Spatially resolved epigenome-transcriptome co-profiling of mammalian tissues at the cellular level',
'authors' => 'Fan Rong et al.',
'description' => '<p>Emerging spatial technologies including spatial transcriptomics and spatial epigenomics are becoming powerful tools for profiling cellular states in the tissue context. However, current methods capture only one layer of omics information at a time precluding the possibility to examine the mechanistic relationship across the cental dogma of molecular biology. Here, we present two spatial multi-omics technologies for spatially resolved genome-wide joint mapping of epigenome and transcriptome by coprofiling chromatin accessibility and gene expression (spatial-ATAC-RNA-seq) or histone modification and gene expression (spatial-CUT\&Tag-RNA-seq) on the same tissue section at a resolution near single cells. They were applied to embryonic and neonatal mouse brain as well as adult human brain to map how epigenetic states or modifications regulate cell type and dynamics in tissue. Although distinct tissue features were identified by either spatial epigenome or spatial transcriptome alone with high concordance, we observed their differential roles in defining cell states. In general, epigenetic state proceeds the development of transcriptional phenotype in relation to epigenetic lineage priming. We also observed high expression canonical markers such as PROX1 in the granular cell layer of the human hippocampus showed low chromatin accessibility that corresponded to a low level of RNA turnover rate, highlighting a divergent need for open chromatin or transcription to control cell identity and dynamics. Spatial epigenome-transcriptome co-profiling is a highly informative tool to study the mechanism of gene expression regulation in tissue and may enable a wide range of applications in life science and biomedical research.</p>',
'date' => '2022-06-13',
'pmid' => 'https://www.researchsquare.com/article/rs-1728747/v1',
'doi' => '10.21203/rs.3.rs-1728747/v1',
'modified' => '2022-08-11 15:20:45',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '4408',
'name' => 'Spatially resolved epigenomic profiling of single cells in complex tissues',
'authors' => 'Lu Tian et al.',
'description' => '<p>The recent development of spatial omics methods enables single-cell profiling of the transcriptome and the 3D genome organization in a spatially resolved manner. Expanding the repertoire of spatial omics tools, a spatial epigenomics method will accelerate our understanding of the spatial regulation of cell and tissue functions. Here, we report a method for spatially resolved profiling of epigenomes in single cells using in-situ tagmentation and transcription followed by highly multiplexed imaging. We profiled histone modifications marking active promoters and enhancers, H3K4me3 and H3K27ac, and generated high-resolution spatial atlas of hundreds of active promoters and putative enhancers in embryonic and adult mouse brains. Our results further revealed putative promoter-enhancer pairs and enhancer hubs regulating the expression of developmentally important genes. We envision this approach will be generally applicable to spatial profiling of epigenetic modifications and DNA-binding proteins, advancing our understanding of how gene expression is spatiotemporally regulated by the epigenome.</p>',
'date' => '2022-02-01',
'pmid' => 'https://doi.org/10.1101%2F2022.02.17.480825',
'doi' => '10.1101/2022.02.17.480825',
'modified' => '2022-08-11 15:16:56',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '4831',
'name' => 'PIWI-mediated control of tissue-specific transposons is essential for somatic cell differentiation',
'authors' => 'Danyan Li, David H Taylor, Josien C van Wolfswinkel',
'description' => '<p>PIWI proteins are known as mediators of transposon silencing in animal germlines but are also found in adult pluripotent stem cells of highly regenerative animals, where they are essential for regeneration. Study of the nuclear PIWI protein SMEDWI-2 in the planarian somatic stem cell system reveals an intricate interplay between transposons and cell differentiation in which a subset of transposons is inevitably activated during cell differentiation, and the PIWI protein is required to regain control. Absence of SMEDWI-2 leads to tissue-specific transposon derepression related to cell-type-specific chromatin remodeling events and in addition causes reduced accessibility of lineage-specific genes and defective cell differentiation, resulting in fatal tissue dysfunction. Finally, we show that additional PIWI proteins provide a stem-cell-specific second layer of protection in planarian neoblasts. These findings reveal a far-reaching role of PIWI proteins and PIWI-interacting RNAs (piRNAs) in stem cell biology and cell differentiation.</p>',
'date' => '2021-10-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34610311',
'doi' => '10.1016/j.celrep.2021.109776',
'modified' => '2023-08-01 14:21:10',
'created' => '2023-08-01 15:59:38',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '214',
'name' => 'pA-Tn5 Transposase - unloaded SDS GB en',
'language' => 'en',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-GB-en-GHS_1_0.pdf',
'countries' => 'GB',
'modified' => '2020-06-08 16:13:25',
'created' => '2020-06-08 16:13:25',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '216',
'name' => 'pA-Tn5 Transposase - unloaded SDS US en',
'language' => 'en',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2020-06-08 16:15:02',
'created' => '2020-06-08 16:15:02',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '211',
'name' => 'pA-Tn5 Transposase - unloaded SDS DE de',
'language' => 'de',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded--DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2020-06-08 16:11:36',
'created' => '2020-06-08 16:11:36',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '215',
'name' => 'pA-Tn5 Transposase - unloaded SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-JP-ja-GHS_2_0.pdf',
'countries' => 'JP',
'modified' => '2020-06-08 16:14:15',
'created' => '2020-06-08 16:14:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '210',
'name' => 'pA-Tn5 Transposase - unloaded SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2020-06-08 16:10:56',
'created' => '2020-06-08 16:10:56',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '213',
'name' => 'pA-Tn5 Transposase - unloaded SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2020-06-08 16:12:46',
'created' => '2020-06-08 16:12:46',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '209',
'name' => 'pA-Tn5 Transposase - unloaded SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2020-06-08 16:10:09',
'created' => '2020-06-08 16:10:09',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '212',
'name' => 'pA-Tn5 Transposase - unloaded SDS ES es',
'language' => 'es',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2020-06-08 16:12:16',
'created' => '2020-06-08 16:12:16',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/jp/p/pa-tn5-transposase-unloaded'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3065',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µl',
'catalog_number' => 'C01070002',
'old_catalog_number' => '',
'sf_code' => 'C01070002-19650',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '650',
'price_USD' => '650',
'price_GBP' => '575',
'price_JPY' => '/',
'price_CNY' => '/',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded',
'meta_title' => 'pA-Tn5 Transposase (unloaded) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:36:57',
'created' => '2020-04-28 16:21:20'
)
)
$pro = array(
'id' => '3067',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '30 µl',
'catalog_number' => 'C01070002-30',
'old_catalog_number' => '',
'sf_code' => 'C01070002-143301',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1155',
'price_USD' => '1155',
'price_GBP' => '1010',
'price_JPY' => '/',
'price_CNY' => '0',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded-30-ul',
'meta_title' => 'pA-Tn5 Transposase (unloaded)| Diagenode.com',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:37:23',
'created' => '2020-04-28 16:56:21',
'ProductsGroup' => array(
'id' => '312',
'product_id' => '3067',
'group_id' => '275'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '3065',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µl',
'catalog_number' => 'C01070002',
'old_catalog_number' => '',
'sf_code' => 'C01070002-19650',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '650',
'price_USD' => '650',
'price_GBP' => '575',
'price_JPY' => '/',
'price_CNY' => '/',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded',
'meta_title' => 'pA-Tn5 Transposase (unloaded) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:36:57',
'created' => '2020-04-28 16:21:20'
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '1077',
'name' => 'Transposome Assembly Using Diagenode pA-Tn5 Transposase',
'description' => '<p>pA-Tn5 transposase (Cat. No. C01070002) is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For flexibility of use, the fusion protein is not pre-loaded with sequencing adapters. The fusion protein should be loaded with appropriate oligonucleotides prior to use. Oligonucleotides should contain 19-mer Tn5 mosaic ends (underlined) recognized by the transposase and the sequences (bold) allowing the PCR amplification with Illumina-compatible barcoded i7/i5 primers. These sequences have to be adapted to a particular experimental design and take into account the sequencing platform requirements.</p>',
'image_id' => '246',
'type' => 'Protocol',
'url' => 'files/products/cutandtag/assembly-protocol-pAtn5-20042020-rms.pdf',
'slug' => 'assembly-protocol-patn5-20042020-rms',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-04-28 16:32:44',
'created' => '2020-04-28 16:32:44',
'ProductsDocument' => array(
'id' => '2878',
'product_id' => '3067',
'document_id' => '1077'
)
)
$sds = array(
'id' => '212',
'name' => 'pA-Tn5 Transposase - unloaded SDS ES es',
'language' => 'es',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2020-06-08 16:12:16',
'created' => '2020-06-08 16:12:16',
'ProductsSafetySheet' => array(
'id' => '408',
'product_id' => '3067',
'safety_sheet_id' => '212'
)
)
$publication = array(
'id' => '4831',
'name' => 'PIWI-mediated control of tissue-specific transposons is essential for somatic cell differentiation',
'authors' => 'Danyan Li, David H Taylor, Josien C van Wolfswinkel',
'description' => '<p>PIWI proteins are known as mediators of transposon silencing in animal germlines but are also found in adult pluripotent stem cells of highly regenerative animals, where they are essential for regeneration. Study of the nuclear PIWI protein SMEDWI-2 in the planarian somatic stem cell system reveals an intricate interplay between transposons and cell differentiation in which a subset of transposons is inevitably activated during cell differentiation, and the PIWI protein is required to regain control. Absence of SMEDWI-2 leads to tissue-specific transposon derepression related to cell-type-specific chromatin remodeling events and in addition causes reduced accessibility of lineage-specific genes and defective cell differentiation, resulting in fatal tissue dysfunction. Finally, we show that additional PIWI proteins provide a stem-cell-specific second layer of protection in planarian neoblasts. These findings reveal a far-reaching role of PIWI proteins and PIWI-interacting RNAs (piRNAs) in stem cell biology and cell differentiation.</p>',
'date' => '2021-10-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34610311',
'doi' => '10.1016/j.celrep.2021.109776',
'modified' => '2023-08-01 14:21:10',
'created' => '2023-08-01 15:59:38',
'ProductsPublication' => array(
'id' => '7033',
'product_id' => '3067',
'publication_id' => '4831'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/34610311" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase - unloaded',
'meta_title' => 'pA-Tn5 Transposase - unloaded',
'product' => array(
'Product' => array(
'id' => '3067',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '30 µl',
'catalog_number' => 'C01070002-30',
'old_catalog_number' => '',
'sf_code' => 'C01070002-143301',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1155',
'price_USD' => '1155',
'price_GBP' => '1010',
'price_JPY' => '/',
'price_CNY' => '0',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded-30-ul',
'meta_title' => 'pA-Tn5 Transposase - unloaded',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase - unloaded',
'modified' => '2024-08-29 09:37:23',
'created' => '2020-04-28 16:56:21',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/jp/p/pa-tn5-transposase-unloaded'
)
$language = 'jp'
$meta_keywords = ''
$meta_description = 'pA-Tn5 Transposase - unloaded'
$meta_title = 'pA-Tn5 Transposase - unloaded'
$product = array(
'Product' => array(
'id' => '3067',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '30 µl',
'catalog_number' => 'C01070002-30',
'old_catalog_number' => '',
'sf_code' => 'C01070002-143301',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1155',
'price_USD' => '1155',
'price_GBP' => '1010',
'price_JPY' => '/',
'price_CNY' => '0',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded-30-ul',
'meta_title' => 'pA-Tn5 Transposase - unloaded',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase - unloaded',
'modified' => '2024-08-29 09:37:23',
'created' => '2020-04-28 16:56:21',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '275',
'name' => 'C01070002',
'product_id' => '3065',
'modified' => '2020-04-28 16:56:42',
'created' => '2020-04-28 16:56:42'
),
'Master' => array(
'id' => '3065',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µl',
'catalog_number' => 'C01070002',
'old_catalog_number' => '',
'sf_code' => 'C01070002-19650',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '650',
'price_USD' => '650',
'price_GBP' => '575',
'price_JPY' => '/',
'price_CNY' => '/',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded',
'meta_title' => 'pA-Tn5 Transposase (unloaded) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:36:57',
'created' => '2020-04-28 16:21:20'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(
(int) 0 => array(
'id' => '142',
'position' => '4',
'parent_id' => '13',
'name' => 'CUT&Tag',
'description' => '<p><img alt=" iDeal CUT&Tag for Histones" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></p>
<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><strong>CUT&Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&Tag – our iDeal CUT&Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein – pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&Tag</a> proving their high performance in this assay.</p>
<br /> <a href="https://www.diagenode.com/files/application_notes/AN-iDealCUTandTag.pdf"><img src="https://www.diagenode.com/img/banners/cutandtag-appnote.png" /></a><br /><br /></div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); }
// ]]></script>
</center></div>
</div>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a>
<div id="v5" class="content">
<p>The iDeal CUT&Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode’s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode’s Primer Indexes for tagmented libraries.</p>
<img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div>
<h2>Products for CUT&Tag assay</h2>
<h3 class="diacol">Complete solutions</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&Tag kit for Histones</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">Fusion protein</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li>
</ul>
<h3 class="diacol">CUT&Tag grade antibodies</h3>
<ul class="nobullet">
<li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&Tag</a></li>
<li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li>
<li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&Tag</a></li>
</ul>
<h3 class="diacol">Positive & Negative CUT&Tag control</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">DNA purification</h3>
<p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p>
<h3 class="diacol">Sequencing indexes</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li>
</ul>
</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'cutandtag',
'cookies_tag_id' => null,
'meta_keywords' => '',
'meta_description' => 'Rapid chromatin profiling assay, optimized protocol, all reagents provided. Easy sample handling due to ConA magnetic beads. Highly specific CUT&Tag grade antibodies available. ',
'meta_title' => 'Diagenode provides advanced solutions for high performance CUT&Tag experiments',
'modified' => '2024-03-07 17:14:34',
'created' => '2020-04-28 15:07:36',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '1079',
'name' => 'pA-TN5 transposase (unloaded) datasheet',
'description' => '<p>pA-Tn5 transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For flexibility of use, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended assembly protocol prior to use in CUT&Tag or similar assays.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/cutandtag/C01070002-pa-tn5-transposase-unloaded.pdf',
'slug' => 'c01070002-pa-tn5-transposase-unloaded-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-04-28 16:45:02',
'created' => '2020-04-28 16:45:02',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1077',
'name' => 'Transposome Assembly Using Diagenode pA-Tn5 Transposase',
'description' => '<p>pA-Tn5 transposase (Cat. No. C01070002) is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For flexibility of use, the fusion protein is not pre-loaded with sequencing adapters. The fusion protein should be loaded with appropriate oligonucleotides prior to use. Oligonucleotides should contain 19-mer Tn5 mosaic ends (underlined) recognized by the transposase and the sequences (bold) allowing the PCR amplification with Illumina-compatible barcoded i7/i5 primers. These sequences have to be adapted to a particular experimental design and take into account the sequencing platform requirements.</p>',
'image_id' => '246',
'type' => 'Protocol',
'url' => 'files/products/cutandtag/assembly-protocol-pAtn5-20042020-rms.pdf',
'slug' => 'assembly-protocol-patn5-20042020-rms',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-04-28 16:32:44',
'created' => '2020-04-28 16:32:44',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4781',
'name' => 'Spatial epigenome-transcriptome co-profiling of mammalian tissues.',
'authors' => 'Zhang D. et al.',
'description' => '<p>Emerging spatial technologies, including spatial transcriptomics and spatial epigenomics, are becoming powerful tools for profiling of cellular states in the tissue context. However, current methods capture only one layer of omics information at a time, precluding the possibility of examining the mechanistic relationship across the central dogma of molecular biology. Here, we present two technologies for spatially resolved, genome-wide, joint profiling of the epigenome and transcriptome by cosequencing chromatin accessibility and gene expression, or histone modifications (H3K27me3, H3K27ac or H3K4me3) and gene expression on the same tissue section at near-single-cell resolution. These were applied to embryonic and juvenile mouse brain, as well as adult human brain, to map how epigenetic mechanisms control transcriptional phenotype and cell dynamics in tissue. Although highly concordant tissue features were identified by either spatial epigenome or spatial transcriptome we also observed distinct patterns, suggesting their differential roles in defining cell states. Linking epigenome to transcriptome pixel by pixel allows the uncovering of new insights in spatial epigenetic priming, differentiation and gene regulation within the tissue architecture. These technologies are of great interest in life science and biomedical research.</p>',
'date' => '2023-04-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36922587',
'doi' => '10.1038/s41586-023-05795-1',
'modified' => '2023-06-13 09:17:26',
'created' => '2023-05-05 12:34:24',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '4389',
'name' => 'Spatially resolved epigenome-transcriptome co-profiling of mammalian tissues at the cellular level',
'authors' => 'Fan Rong et al.',
'description' => '<p>Emerging spatial technologies including spatial transcriptomics and spatial epigenomics are becoming powerful tools for profiling cellular states in the tissue context. However, current methods capture only one layer of omics information at a time precluding the possibility to examine the mechanistic relationship across the cental dogma of molecular biology. Here, we present two spatial multi-omics technologies for spatially resolved genome-wide joint mapping of epigenome and transcriptome by coprofiling chromatin accessibility and gene expression (spatial-ATAC-RNA-seq) or histone modification and gene expression (spatial-CUT\&Tag-RNA-seq) on the same tissue section at a resolution near single cells. They were applied to embryonic and neonatal mouse brain as well as adult human brain to map how epigenetic states or modifications regulate cell type and dynamics in tissue. Although distinct tissue features were identified by either spatial epigenome or spatial transcriptome alone with high concordance, we observed their differential roles in defining cell states. In general, epigenetic state proceeds the development of transcriptional phenotype in relation to epigenetic lineage priming. We also observed high expression canonical markers such as PROX1 in the granular cell layer of the human hippocampus showed low chromatin accessibility that corresponded to a low level of RNA turnover rate, highlighting a divergent need for open chromatin or transcription to control cell identity and dynamics. Spatial epigenome-transcriptome co-profiling is a highly informative tool to study the mechanism of gene expression regulation in tissue and may enable a wide range of applications in life science and biomedical research.</p>',
'date' => '2022-06-13',
'pmid' => 'https://www.researchsquare.com/article/rs-1728747/v1',
'doi' => '10.21203/rs.3.rs-1728747/v1',
'modified' => '2022-08-11 15:20:45',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '4408',
'name' => 'Spatially resolved epigenomic profiling of single cells in complex tissues',
'authors' => 'Lu Tian et al.',
'description' => '<p>The recent development of spatial omics methods enables single-cell profiling of the transcriptome and the 3D genome organization in a spatially resolved manner. Expanding the repertoire of spatial omics tools, a spatial epigenomics method will accelerate our understanding of the spatial regulation of cell and tissue functions. Here, we report a method for spatially resolved profiling of epigenomes in single cells using in-situ tagmentation and transcription followed by highly multiplexed imaging. We profiled histone modifications marking active promoters and enhancers, H3K4me3 and H3K27ac, and generated high-resolution spatial atlas of hundreds of active promoters and putative enhancers in embryonic and adult mouse brains. Our results further revealed putative promoter-enhancer pairs and enhancer hubs regulating the expression of developmentally important genes. We envision this approach will be generally applicable to spatial profiling of epigenetic modifications and DNA-binding proteins, advancing our understanding of how gene expression is spatiotemporally regulated by the epigenome.</p>',
'date' => '2022-02-01',
'pmid' => 'https://doi.org/10.1101%2F2022.02.17.480825',
'doi' => '10.1101/2022.02.17.480825',
'modified' => '2022-08-11 15:16:56',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '4831',
'name' => 'PIWI-mediated control of tissue-specific transposons is essential for somatic cell differentiation',
'authors' => 'Danyan Li, David H Taylor, Josien C van Wolfswinkel',
'description' => '<p>PIWI proteins are known as mediators of transposon silencing in animal germlines but are also found in adult pluripotent stem cells of highly regenerative animals, where they are essential for regeneration. Study of the nuclear PIWI protein SMEDWI-2 in the planarian somatic stem cell system reveals an intricate interplay between transposons and cell differentiation in which a subset of transposons is inevitably activated during cell differentiation, and the PIWI protein is required to regain control. Absence of SMEDWI-2 leads to tissue-specific transposon derepression related to cell-type-specific chromatin remodeling events and in addition causes reduced accessibility of lineage-specific genes and defective cell differentiation, resulting in fatal tissue dysfunction. Finally, we show that additional PIWI proteins provide a stem-cell-specific second layer of protection in planarian neoblasts. These findings reveal a far-reaching role of PIWI proteins and PIWI-interacting RNAs (piRNAs) in stem cell biology and cell differentiation.</p>',
'date' => '2021-10-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34610311',
'doi' => '10.1016/j.celrep.2021.109776',
'modified' => '2023-08-01 14:21:10',
'created' => '2023-08-01 15:59:38',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '214',
'name' => 'pA-Tn5 Transposase - unloaded SDS GB en',
'language' => 'en',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-GB-en-GHS_1_0.pdf',
'countries' => 'GB',
'modified' => '2020-06-08 16:13:25',
'created' => '2020-06-08 16:13:25',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '216',
'name' => 'pA-Tn5 Transposase - unloaded SDS US en',
'language' => 'en',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2020-06-08 16:15:02',
'created' => '2020-06-08 16:15:02',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '211',
'name' => 'pA-Tn5 Transposase - unloaded SDS DE de',
'language' => 'de',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded--DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2020-06-08 16:11:36',
'created' => '2020-06-08 16:11:36',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '215',
'name' => 'pA-Tn5 Transposase - unloaded SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-JP-ja-GHS_2_0.pdf',
'countries' => 'JP',
'modified' => '2020-06-08 16:14:15',
'created' => '2020-06-08 16:14:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '210',
'name' => 'pA-Tn5 Transposase - unloaded SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2020-06-08 16:10:56',
'created' => '2020-06-08 16:10:56',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '213',
'name' => 'pA-Tn5 Transposase - unloaded SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2020-06-08 16:12:46',
'created' => '2020-06-08 16:12:46',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '209',
'name' => 'pA-Tn5 Transposase - unloaded SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2020-06-08 16:10:09',
'created' => '2020-06-08 16:10:09',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '212',
'name' => 'pA-Tn5 Transposase - unloaded SDS ES es',
'language' => 'es',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2020-06-08 16:12:16',
'created' => '2020-06-08 16:12:16',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/jp/p/pa-tn5-transposase-unloaded'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3065',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µl',
'catalog_number' => 'C01070002',
'old_catalog_number' => '',
'sf_code' => 'C01070002-19650',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '650',
'price_USD' => '650',
'price_GBP' => '575',
'price_JPY' => '/',
'price_CNY' => '/',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded',
'meta_title' => 'pA-Tn5 Transposase (unloaded) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:36:57',
'created' => '2020-04-28 16:21:20'
)
)
$pro = array(
'id' => '3067',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '30 µl',
'catalog_number' => 'C01070002-30',
'old_catalog_number' => '',
'sf_code' => 'C01070002-143301',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1155',
'price_USD' => '1155',
'price_GBP' => '1010',
'price_JPY' => '/',
'price_CNY' => '0',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded-30-ul',
'meta_title' => 'pA-Tn5 Transposase (unloaded)| Diagenode.com',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:37:23',
'created' => '2020-04-28 16:56:21',
'ProductsGroup' => array(
'id' => '312',
'product_id' => '3067',
'group_id' => '275'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '3065',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µl',
'catalog_number' => 'C01070002',
'old_catalog_number' => '',
'sf_code' => 'C01070002-19650',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '650',
'price_USD' => '650',
'price_GBP' => '575',
'price_JPY' => '/',
'price_CNY' => '/',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded',
'meta_title' => 'pA-Tn5 Transposase (unloaded) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:36:57',
'created' => '2020-04-28 16:21:20'
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '1077',
'name' => 'Transposome Assembly Using Diagenode pA-Tn5 Transposase',
'description' => '<p>pA-Tn5 transposase (Cat. No. C01070002) is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For flexibility of use, the fusion protein is not pre-loaded with sequencing adapters. The fusion protein should be loaded with appropriate oligonucleotides prior to use. Oligonucleotides should contain 19-mer Tn5 mosaic ends (underlined) recognized by the transposase and the sequences (bold) allowing the PCR amplification with Illumina-compatible barcoded i7/i5 primers. These sequences have to be adapted to a particular experimental design and take into account the sequencing platform requirements.</p>',
'image_id' => '246',
'type' => 'Protocol',
'url' => 'files/products/cutandtag/assembly-protocol-pAtn5-20042020-rms.pdf',
'slug' => 'assembly-protocol-patn5-20042020-rms',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-04-28 16:32:44',
'created' => '2020-04-28 16:32:44',
'ProductsDocument' => array(
'id' => '2878',
'product_id' => '3067',
'document_id' => '1077'
)
)
$sds = array(
'id' => '212',
'name' => 'pA-Tn5 Transposase - unloaded SDS ES es',
'language' => 'es',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2020-06-08 16:12:16',
'created' => '2020-06-08 16:12:16',
'ProductsSafetySheet' => array(
'id' => '408',
'product_id' => '3067',
'safety_sheet_id' => '212'
)
)
$publication = array(
'id' => '4831',
'name' => 'PIWI-mediated control of tissue-specific transposons is essential for somatic cell differentiation',
'authors' => 'Danyan Li, David H Taylor, Josien C van Wolfswinkel',
'description' => '<p>PIWI proteins are known as mediators of transposon silencing in animal germlines but are also found in adult pluripotent stem cells of highly regenerative animals, where they are essential for regeneration. Study of the nuclear PIWI protein SMEDWI-2 in the planarian somatic stem cell system reveals an intricate interplay between transposons and cell differentiation in which a subset of transposons is inevitably activated during cell differentiation, and the PIWI protein is required to regain control. Absence of SMEDWI-2 leads to tissue-specific transposon derepression related to cell-type-specific chromatin remodeling events and in addition causes reduced accessibility of lineage-specific genes and defective cell differentiation, resulting in fatal tissue dysfunction. Finally, we show that additional PIWI proteins provide a stem-cell-specific second layer of protection in planarian neoblasts. These findings reveal a far-reaching role of PIWI proteins and PIWI-interacting RNAs (piRNAs) in stem cell biology and cell differentiation.</p>',
'date' => '2021-10-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34610311',
'doi' => '10.1016/j.celrep.2021.109776',
'modified' => '2023-08-01 14:21:10',
'created' => '2023-08-01 15:59:38',
'ProductsPublication' => array(
'id' => '7033',
'product_id' => '3067',
'publication_id' => '4831'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/34610311" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase - unloaded',
'meta_title' => 'pA-Tn5 Transposase - unloaded',
'product' => array(
'Product' => array(
'id' => '3067',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '30 µl',
'catalog_number' => 'C01070002-30',
'old_catalog_number' => '',
'sf_code' => 'C01070002-143301',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1155',
'price_USD' => '1155',
'price_GBP' => '1010',
'price_JPY' => '/',
'price_CNY' => '0',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded-30-ul',
'meta_title' => 'pA-Tn5 Transposase - unloaded',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase - unloaded',
'modified' => '2024-08-29 09:37:23',
'created' => '2020-04-28 16:56:21',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/jp/p/pa-tn5-transposase-unloaded'
)
$language = 'jp'
$meta_keywords = ''
$meta_description = 'pA-Tn5 Transposase - unloaded'
$meta_title = 'pA-Tn5 Transposase - unloaded'
$product = array(
'Product' => array(
'id' => '3067',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '30 µl',
'catalog_number' => 'C01070002-30',
'old_catalog_number' => '',
'sf_code' => 'C01070002-143301',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1155',
'price_USD' => '1155',
'price_GBP' => '1010',
'price_JPY' => '/',
'price_CNY' => '0',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded-30-ul',
'meta_title' => 'pA-Tn5 Transposase - unloaded',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase - unloaded',
'modified' => '2024-08-29 09:37:23',
'created' => '2020-04-28 16:56:21',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '275',
'name' => 'C01070002',
'product_id' => '3065',
'modified' => '2020-04-28 16:56:42',
'created' => '2020-04-28 16:56:42'
),
'Master' => array(
'id' => '3065',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µl',
'catalog_number' => 'C01070002',
'old_catalog_number' => '',
'sf_code' => 'C01070002-19650',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '650',
'price_USD' => '650',
'price_GBP' => '575',
'price_JPY' => '/',
'price_CNY' => '/',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded',
'meta_title' => 'pA-Tn5 Transposase (unloaded) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:36:57',
'created' => '2020-04-28 16:21:20'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(
(int) 0 => array(
'id' => '142',
'position' => '4',
'parent_id' => '13',
'name' => 'CUT&Tag',
'description' => '<p><img alt=" iDeal CUT&Tag for Histones" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></p>
<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><strong>CUT&Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&Tag – our iDeal CUT&Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein – pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&Tag</a> proving their high performance in this assay.</p>
<br /> <a href="https://www.diagenode.com/files/application_notes/AN-iDealCUTandTag.pdf"><img src="https://www.diagenode.com/img/banners/cutandtag-appnote.png" /></a><br /><br /></div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); }
// ]]></script>
</center></div>
</div>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a>
<div id="v5" class="content">
<p>The iDeal CUT&Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode’s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode’s Primer Indexes for tagmented libraries.</p>
<img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div>
<h2>Products for CUT&Tag assay</h2>
<h3 class="diacol">Complete solutions</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&Tag kit for Histones</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">Fusion protein</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li>
</ul>
<h3 class="diacol">CUT&Tag grade antibodies</h3>
<ul class="nobullet">
<li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&Tag</a></li>
<li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li>
<li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&Tag</a></li>
</ul>
<h3 class="diacol">Positive & Negative CUT&Tag control</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">DNA purification</h3>
<p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p>
<h3 class="diacol">Sequencing indexes</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li>
</ul>
</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'cutandtag',
'cookies_tag_id' => null,
'meta_keywords' => '',
'meta_description' => 'Rapid chromatin profiling assay, optimized protocol, all reagents provided. Easy sample handling due to ConA magnetic beads. Highly specific CUT&Tag grade antibodies available. ',
'meta_title' => 'Diagenode provides advanced solutions for high performance CUT&Tag experiments',
'modified' => '2024-03-07 17:14:34',
'created' => '2020-04-28 15:07:36',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '1079',
'name' => 'pA-TN5 transposase (unloaded) datasheet',
'description' => '<p>pA-Tn5 transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For flexibility of use, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended assembly protocol prior to use in CUT&Tag or similar assays.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/cutandtag/C01070002-pa-tn5-transposase-unloaded.pdf',
'slug' => 'c01070002-pa-tn5-transposase-unloaded-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-04-28 16:45:02',
'created' => '2020-04-28 16:45:02',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1077',
'name' => 'Transposome Assembly Using Diagenode pA-Tn5 Transposase',
'description' => '<p>pA-Tn5 transposase (Cat. No. C01070002) is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For flexibility of use, the fusion protein is not pre-loaded with sequencing adapters. The fusion protein should be loaded with appropriate oligonucleotides prior to use. Oligonucleotides should contain 19-mer Tn5 mosaic ends (underlined) recognized by the transposase and the sequences (bold) allowing the PCR amplification with Illumina-compatible barcoded i7/i5 primers. These sequences have to be adapted to a particular experimental design and take into account the sequencing platform requirements.</p>',
'image_id' => '246',
'type' => 'Protocol',
'url' => 'files/products/cutandtag/assembly-protocol-pAtn5-20042020-rms.pdf',
'slug' => 'assembly-protocol-patn5-20042020-rms',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-04-28 16:32:44',
'created' => '2020-04-28 16:32:44',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4781',
'name' => 'Spatial epigenome-transcriptome co-profiling of mammalian tissues.',
'authors' => 'Zhang D. et al.',
'description' => '<p>Emerging spatial technologies, including spatial transcriptomics and spatial epigenomics, are becoming powerful tools for profiling of cellular states in the tissue context. However, current methods capture only one layer of omics information at a time, precluding the possibility of examining the mechanistic relationship across the central dogma of molecular biology. Here, we present two technologies for spatially resolved, genome-wide, joint profiling of the epigenome and transcriptome by cosequencing chromatin accessibility and gene expression, or histone modifications (H3K27me3, H3K27ac or H3K4me3) and gene expression on the same tissue section at near-single-cell resolution. These were applied to embryonic and juvenile mouse brain, as well as adult human brain, to map how epigenetic mechanisms control transcriptional phenotype and cell dynamics in tissue. Although highly concordant tissue features were identified by either spatial epigenome or spatial transcriptome we also observed distinct patterns, suggesting their differential roles in defining cell states. Linking epigenome to transcriptome pixel by pixel allows the uncovering of new insights in spatial epigenetic priming, differentiation and gene regulation within the tissue architecture. These technologies are of great interest in life science and biomedical research.</p>',
'date' => '2023-04-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36922587',
'doi' => '10.1038/s41586-023-05795-1',
'modified' => '2023-06-13 09:17:26',
'created' => '2023-05-05 12:34:24',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '4389',
'name' => 'Spatially resolved epigenome-transcriptome co-profiling of mammalian tissues at the cellular level',
'authors' => 'Fan Rong et al.',
'description' => '<p>Emerging spatial technologies including spatial transcriptomics and spatial epigenomics are becoming powerful tools for profiling cellular states in the tissue context. However, current methods capture only one layer of omics information at a time precluding the possibility to examine the mechanistic relationship across the cental dogma of molecular biology. Here, we present two spatial multi-omics technologies for spatially resolved genome-wide joint mapping of epigenome and transcriptome by coprofiling chromatin accessibility and gene expression (spatial-ATAC-RNA-seq) or histone modification and gene expression (spatial-CUT\&Tag-RNA-seq) on the same tissue section at a resolution near single cells. They were applied to embryonic and neonatal mouse brain as well as adult human brain to map how epigenetic states or modifications regulate cell type and dynamics in tissue. Although distinct tissue features were identified by either spatial epigenome or spatial transcriptome alone with high concordance, we observed their differential roles in defining cell states. In general, epigenetic state proceeds the development of transcriptional phenotype in relation to epigenetic lineage priming. We also observed high expression canonical markers such as PROX1 in the granular cell layer of the human hippocampus showed low chromatin accessibility that corresponded to a low level of RNA turnover rate, highlighting a divergent need for open chromatin or transcription to control cell identity and dynamics. Spatial epigenome-transcriptome co-profiling is a highly informative tool to study the mechanism of gene expression regulation in tissue and may enable a wide range of applications in life science and biomedical research.</p>',
'date' => '2022-06-13',
'pmid' => 'https://www.researchsquare.com/article/rs-1728747/v1',
'doi' => '10.21203/rs.3.rs-1728747/v1',
'modified' => '2022-08-11 15:20:45',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '4408',
'name' => 'Spatially resolved epigenomic profiling of single cells in complex tissues',
'authors' => 'Lu Tian et al.',
'description' => '<p>The recent development of spatial omics methods enables single-cell profiling of the transcriptome and the 3D genome organization in a spatially resolved manner. Expanding the repertoire of spatial omics tools, a spatial epigenomics method will accelerate our understanding of the spatial regulation of cell and tissue functions. Here, we report a method for spatially resolved profiling of epigenomes in single cells using in-situ tagmentation and transcription followed by highly multiplexed imaging. We profiled histone modifications marking active promoters and enhancers, H3K4me3 and H3K27ac, and generated high-resolution spatial atlas of hundreds of active promoters and putative enhancers in embryonic and adult mouse brains. Our results further revealed putative promoter-enhancer pairs and enhancer hubs regulating the expression of developmentally important genes. We envision this approach will be generally applicable to spatial profiling of epigenetic modifications and DNA-binding proteins, advancing our understanding of how gene expression is spatiotemporally regulated by the epigenome.</p>',
'date' => '2022-02-01',
'pmid' => 'https://doi.org/10.1101%2F2022.02.17.480825',
'doi' => '10.1101/2022.02.17.480825',
'modified' => '2022-08-11 15:16:56',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '4831',
'name' => 'PIWI-mediated control of tissue-specific transposons is essential for somatic cell differentiation',
'authors' => 'Danyan Li, David H Taylor, Josien C van Wolfswinkel',
'description' => '<p>PIWI proteins are known as mediators of transposon silencing in animal germlines but are also found in adult pluripotent stem cells of highly regenerative animals, where they are essential for regeneration. Study of the nuclear PIWI protein SMEDWI-2 in the planarian somatic stem cell system reveals an intricate interplay between transposons and cell differentiation in which a subset of transposons is inevitably activated during cell differentiation, and the PIWI protein is required to regain control. Absence of SMEDWI-2 leads to tissue-specific transposon derepression related to cell-type-specific chromatin remodeling events and in addition causes reduced accessibility of lineage-specific genes and defective cell differentiation, resulting in fatal tissue dysfunction. Finally, we show that additional PIWI proteins provide a stem-cell-specific second layer of protection in planarian neoblasts. These findings reveal a far-reaching role of PIWI proteins and PIWI-interacting RNAs (piRNAs) in stem cell biology and cell differentiation.</p>',
'date' => '2021-10-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34610311',
'doi' => '10.1016/j.celrep.2021.109776',
'modified' => '2023-08-01 14:21:10',
'created' => '2023-08-01 15:59:38',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '214',
'name' => 'pA-Tn5 Transposase - unloaded SDS GB en',
'language' => 'en',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-GB-en-GHS_1_0.pdf',
'countries' => 'GB',
'modified' => '2020-06-08 16:13:25',
'created' => '2020-06-08 16:13:25',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '216',
'name' => 'pA-Tn5 Transposase - unloaded SDS US en',
'language' => 'en',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2020-06-08 16:15:02',
'created' => '2020-06-08 16:15:02',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '211',
'name' => 'pA-Tn5 Transposase - unloaded SDS DE de',
'language' => 'de',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded--DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2020-06-08 16:11:36',
'created' => '2020-06-08 16:11:36',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '215',
'name' => 'pA-Tn5 Transposase - unloaded SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-JP-ja-GHS_2_0.pdf',
'countries' => 'JP',
'modified' => '2020-06-08 16:14:15',
'created' => '2020-06-08 16:14:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '210',
'name' => 'pA-Tn5 Transposase - unloaded SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2020-06-08 16:10:56',
'created' => '2020-06-08 16:10:56',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '213',
'name' => 'pA-Tn5 Transposase - unloaded SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2020-06-08 16:12:46',
'created' => '2020-06-08 16:12:46',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '209',
'name' => 'pA-Tn5 Transposase - unloaded SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2020-06-08 16:10:09',
'created' => '2020-06-08 16:10:09',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '212',
'name' => 'pA-Tn5 Transposase - unloaded SDS ES es',
'language' => 'es',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2020-06-08 16:12:16',
'created' => '2020-06-08 16:12:16',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/jp/p/pa-tn5-transposase-unloaded'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3065',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µl',
'catalog_number' => 'C01070002',
'old_catalog_number' => '',
'sf_code' => 'C01070002-19650',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '650',
'price_USD' => '650',
'price_GBP' => '575',
'price_JPY' => '/',
'price_CNY' => '/',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded',
'meta_title' => 'pA-Tn5 Transposase (unloaded) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:36:57',
'created' => '2020-04-28 16:21:20'
)
)
$pro = array(
'id' => '3067',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '30 µl',
'catalog_number' => 'C01070002-30',
'old_catalog_number' => '',
'sf_code' => 'C01070002-143301',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1155',
'price_USD' => '1155',
'price_GBP' => '1010',
'price_JPY' => '/',
'price_CNY' => '0',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded-30-ul',
'meta_title' => 'pA-Tn5 Transposase (unloaded)| Diagenode.com',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:37:23',
'created' => '2020-04-28 16:56:21',
'ProductsGroup' => array(
'id' => '312',
'product_id' => '3067',
'group_id' => '275'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '3065',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µl',
'catalog_number' => 'C01070002',
'old_catalog_number' => '',
'sf_code' => 'C01070002-19650',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '650',
'price_USD' => '650',
'price_GBP' => '575',
'price_JPY' => '/',
'price_CNY' => '/',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded',
'meta_title' => 'pA-Tn5 Transposase (unloaded) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:36:57',
'created' => '2020-04-28 16:21:20'
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '1077',
'name' => 'Transposome Assembly Using Diagenode pA-Tn5 Transposase',
'description' => '<p>pA-Tn5 transposase (Cat. No. C01070002) is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For flexibility of use, the fusion protein is not pre-loaded with sequencing adapters. The fusion protein should be loaded with appropriate oligonucleotides prior to use. Oligonucleotides should contain 19-mer Tn5 mosaic ends (underlined) recognized by the transposase and the sequences (bold) allowing the PCR amplification with Illumina-compatible barcoded i7/i5 primers. These sequences have to be adapted to a particular experimental design and take into account the sequencing platform requirements.</p>',
'image_id' => '246',
'type' => 'Protocol',
'url' => 'files/products/cutandtag/assembly-protocol-pAtn5-20042020-rms.pdf',
'slug' => 'assembly-protocol-patn5-20042020-rms',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-04-28 16:32:44',
'created' => '2020-04-28 16:32:44',
'ProductsDocument' => array(
'id' => '2878',
'product_id' => '3067',
'document_id' => '1077'
)
)
$sds = array(
'id' => '212',
'name' => 'pA-Tn5 Transposase - unloaded SDS ES es',
'language' => 'es',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2020-06-08 16:12:16',
'created' => '2020-06-08 16:12:16',
'ProductsSafetySheet' => array(
'id' => '408',
'product_id' => '3067',
'safety_sheet_id' => '212'
)
)
$publication = array(
'id' => '4831',
'name' => 'PIWI-mediated control of tissue-specific transposons is essential for somatic cell differentiation',
'authors' => 'Danyan Li, David H Taylor, Josien C van Wolfswinkel',
'description' => '<p>PIWI proteins are known as mediators of transposon silencing in animal germlines but are also found in adult pluripotent stem cells of highly regenerative animals, where they are essential for regeneration. Study of the nuclear PIWI protein SMEDWI-2 in the planarian somatic stem cell system reveals an intricate interplay between transposons and cell differentiation in which a subset of transposons is inevitably activated during cell differentiation, and the PIWI protein is required to regain control. Absence of SMEDWI-2 leads to tissue-specific transposon derepression related to cell-type-specific chromatin remodeling events and in addition causes reduced accessibility of lineage-specific genes and defective cell differentiation, resulting in fatal tissue dysfunction. Finally, we show that additional PIWI proteins provide a stem-cell-specific second layer of protection in planarian neoblasts. These findings reveal a far-reaching role of PIWI proteins and PIWI-interacting RNAs (piRNAs) in stem cell biology and cell differentiation.</p>',
'date' => '2021-10-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34610311',
'doi' => '10.1016/j.celrep.2021.109776',
'modified' => '2023-08-01 14:21:10',
'created' => '2023-08-01 15:59:38',
'ProductsPublication' => array(
'id' => '7033',
'product_id' => '3067',
'publication_id' => '4831'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/34610311" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'jp',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase - unloaded',
'meta_title' => 'pA-Tn5 Transposase - unloaded',
'product' => array(
'Product' => array(
'id' => '3067',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '30 µl',
'catalog_number' => 'C01070002-30',
'old_catalog_number' => '',
'sf_code' => 'C01070002-143301',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1155',
'price_USD' => '1155',
'price_GBP' => '1010',
'price_JPY' => '/',
'price_CNY' => '0',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded-30-ul',
'meta_title' => 'pA-Tn5 Transposase - unloaded',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase - unloaded',
'modified' => '2024-08-29 09:37:23',
'created' => '2020-04-28 16:56:21',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
[maximum depth reached]
),
'Master' => array(
[maximum depth reached]
),
'Product' => array(
[maximum depth reached]
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
),
'meta_canonical' => 'https://www.diagenode.com/jp/p/pa-tn5-transposase-unloaded'
)
$language = 'jp'
$meta_keywords = ''
$meta_description = 'pA-Tn5 Transposase - unloaded'
$meta_title = 'pA-Tn5 Transposase - unloaded'
$product = array(
'Product' => array(
'id' => '3067',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '30 µl',
'catalog_number' => 'C01070002-30',
'old_catalog_number' => '',
'sf_code' => 'C01070002-143301',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1155',
'price_USD' => '1155',
'price_GBP' => '1010',
'price_JPY' => '/',
'price_CNY' => '0',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded-30-ul',
'meta_title' => 'pA-Tn5 Transposase - unloaded',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase - unloaded',
'modified' => '2024-08-29 09:37:23',
'created' => '2020-04-28 16:56:21',
'locale' => 'jpn'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(
'Group' => array(
'id' => '275',
'name' => 'C01070002',
'product_id' => '3065',
'modified' => '2020-04-28 16:56:42',
'created' => '2020-04-28 16:56:42'
),
'Master' => array(
'id' => '3065',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µl',
'catalog_number' => 'C01070002',
'old_catalog_number' => '',
'sf_code' => 'C01070002-19650',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '650',
'price_USD' => '650',
'price_GBP' => '575',
'price_JPY' => '/',
'price_CNY' => '/',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded',
'meta_title' => 'pA-Tn5 Transposase (unloaded) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:36:57',
'created' => '2020-04-28 16:21:20'
),
'Product' => array(
(int) 0 => array(
[maximum depth reached]
)
)
),
'Related' => array(),
'Application' => array(),
'Category' => array(
(int) 0 => array(
'id' => '142',
'position' => '4',
'parent_id' => '13',
'name' => 'CUT&Tag',
'description' => '<p><img alt=" iDeal CUT&Tag for Histones" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></p>
<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><strong>CUT&Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&Tag – our iDeal CUT&Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein – pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&Tag</a> proving their high performance in this assay.</p>
<br /> <a href="https://www.diagenode.com/files/application_notes/AN-iDealCUTandTag.pdf"><img src="https://www.diagenode.com/img/banners/cutandtag-appnote.png" /></a><br /><br /></div>
<div class="small-12 medium-4 large-4 columns"><center>
<script>// <![CDATA[
var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); }
// ]]></script>
</center></div>
</div>
<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a>
<div id="v5" class="content">
<p>The iDeal CUT&Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode’s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode’s Primer Indexes for tagmented libraries.</p>
<img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div>
<h2>Products for CUT&Tag assay</h2>
<h3 class="diacol">Complete solutions</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&Tag kit for Histones</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">Fusion protein</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li>
</ul>
<h3 class="diacol">CUT&Tag grade antibodies</h3>
<ul class="nobullet">
<li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&Tag</a></li>
<li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li>
<li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&Tag</a></li>
</ul>
<h3 class="diacol">Positive & Negative CUT&Tag control</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">DNA purification</h3>
<p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p>
<h3 class="diacol">Sequencing indexes</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li>
</ul>
</li>
</ul>',
'no_promo' => false,
'in_menu' => true,
'online' => true,
'tabular' => false,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'cutandtag',
'cookies_tag_id' => null,
'meta_keywords' => '',
'meta_description' => 'Rapid chromatin profiling assay, optimized protocol, all reagents provided. Easy sample handling due to ConA magnetic beads. Highly specific CUT&Tag grade antibodies available. ',
'meta_title' => 'Diagenode provides advanced solutions for high performance CUT&Tag experiments',
'modified' => '2024-03-07 17:14:34',
'created' => '2020-04-28 15:07:36',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '1079',
'name' => 'pA-TN5 transposase (unloaded) datasheet',
'description' => '<p>pA-Tn5 transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For flexibility of use, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended assembly protocol prior to use in CUT&Tag or similar assays.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/cutandtag/C01070002-pa-tn5-transposase-unloaded.pdf',
'slug' => 'c01070002-pa-tn5-transposase-unloaded-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-04-28 16:45:02',
'created' => '2020-04-28 16:45:02',
'ProductsDocument' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1077',
'name' => 'Transposome Assembly Using Diagenode pA-Tn5 Transposase',
'description' => '<p>pA-Tn5 transposase (Cat. No. C01070002) is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For flexibility of use, the fusion protein is not pre-loaded with sequencing adapters. The fusion protein should be loaded with appropriate oligonucleotides prior to use. Oligonucleotides should contain 19-mer Tn5 mosaic ends (underlined) recognized by the transposase and the sequences (bold) allowing the PCR amplification with Illumina-compatible barcoded i7/i5 primers. These sequences have to be adapted to a particular experimental design and take into account the sequencing platform requirements.</p>',
'image_id' => '246',
'type' => 'Protocol',
'url' => 'files/products/cutandtag/assembly-protocol-pAtn5-20042020-rms.pdf',
'slug' => 'assembly-protocol-patn5-20042020-rms',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-04-28 16:32:44',
'created' => '2020-04-28 16:32:44',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(
(int) 0 => array(
'id' => '4781',
'name' => 'Spatial epigenome-transcriptome co-profiling of mammalian tissues.',
'authors' => 'Zhang D. et al.',
'description' => '<p>Emerging spatial technologies, including spatial transcriptomics and spatial epigenomics, are becoming powerful tools for profiling of cellular states in the tissue context. However, current methods capture only one layer of omics information at a time, precluding the possibility of examining the mechanistic relationship across the central dogma of molecular biology. Here, we present two technologies for spatially resolved, genome-wide, joint profiling of the epigenome and transcriptome by cosequencing chromatin accessibility and gene expression, or histone modifications (H3K27me3, H3K27ac or H3K4me3) and gene expression on the same tissue section at near-single-cell resolution. These were applied to embryonic and juvenile mouse brain, as well as adult human brain, to map how epigenetic mechanisms control transcriptional phenotype and cell dynamics in tissue. Although highly concordant tissue features were identified by either spatial epigenome or spatial transcriptome we also observed distinct patterns, suggesting their differential roles in defining cell states. Linking epigenome to transcriptome pixel by pixel allows the uncovering of new insights in spatial epigenetic priming, differentiation and gene regulation within the tissue architecture. These technologies are of great interest in life science and biomedical research.</p>',
'date' => '2023-04-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36922587',
'doi' => '10.1038/s41586-023-05795-1',
'modified' => '2023-06-13 09:17:26',
'created' => '2023-05-05 12:34:24',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '4389',
'name' => 'Spatially resolved epigenome-transcriptome co-profiling of mammalian tissues at the cellular level',
'authors' => 'Fan Rong et al.',
'description' => '<p>Emerging spatial technologies including spatial transcriptomics and spatial epigenomics are becoming powerful tools for profiling cellular states in the tissue context. However, current methods capture only one layer of omics information at a time precluding the possibility to examine the mechanistic relationship across the cental dogma of molecular biology. Here, we present two spatial multi-omics technologies for spatially resolved genome-wide joint mapping of epigenome and transcriptome by coprofiling chromatin accessibility and gene expression (spatial-ATAC-RNA-seq) or histone modification and gene expression (spatial-CUT\&Tag-RNA-seq) on the same tissue section at a resolution near single cells. They were applied to embryonic and neonatal mouse brain as well as adult human brain to map how epigenetic states or modifications regulate cell type and dynamics in tissue. Although distinct tissue features were identified by either spatial epigenome or spatial transcriptome alone with high concordance, we observed their differential roles in defining cell states. In general, epigenetic state proceeds the development of transcriptional phenotype in relation to epigenetic lineage priming. We also observed high expression canonical markers such as PROX1 in the granular cell layer of the human hippocampus showed low chromatin accessibility that corresponded to a low level of RNA turnover rate, highlighting a divergent need for open chromatin or transcription to control cell identity and dynamics. Spatial epigenome-transcriptome co-profiling is a highly informative tool to study the mechanism of gene expression regulation in tissue and may enable a wide range of applications in life science and biomedical research.</p>',
'date' => '2022-06-13',
'pmid' => 'https://www.researchsquare.com/article/rs-1728747/v1',
'doi' => '10.21203/rs.3.rs-1728747/v1',
'modified' => '2022-08-11 15:20:45',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '4408',
'name' => 'Spatially resolved epigenomic profiling of single cells in complex tissues',
'authors' => 'Lu Tian et al.',
'description' => '<p>The recent development of spatial omics methods enables single-cell profiling of the transcriptome and the 3D genome organization in a spatially resolved manner. Expanding the repertoire of spatial omics tools, a spatial epigenomics method will accelerate our understanding of the spatial regulation of cell and tissue functions. Here, we report a method for spatially resolved profiling of epigenomes in single cells using in-situ tagmentation and transcription followed by highly multiplexed imaging. We profiled histone modifications marking active promoters and enhancers, H3K4me3 and H3K27ac, and generated high-resolution spatial atlas of hundreds of active promoters and putative enhancers in embryonic and adult mouse brains. Our results further revealed putative promoter-enhancer pairs and enhancer hubs regulating the expression of developmentally important genes. We envision this approach will be generally applicable to spatial profiling of epigenetic modifications and DNA-binding proteins, advancing our understanding of how gene expression is spatiotemporally regulated by the epigenome.</p>',
'date' => '2022-02-01',
'pmid' => 'https://doi.org/10.1101%2F2022.02.17.480825',
'doi' => '10.1101/2022.02.17.480825',
'modified' => '2022-08-11 15:16:56',
'created' => '2022-08-11 12:14:50',
'ProductsPublication' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '4831',
'name' => 'PIWI-mediated control of tissue-specific transposons is essential for somatic cell differentiation',
'authors' => 'Danyan Li, David H Taylor, Josien C van Wolfswinkel',
'description' => '<p>PIWI proteins are known as mediators of transposon silencing in animal germlines but are also found in adult pluripotent stem cells of highly regenerative animals, where they are essential for regeneration. Study of the nuclear PIWI protein SMEDWI-2 in the planarian somatic stem cell system reveals an intricate interplay between transposons and cell differentiation in which a subset of transposons is inevitably activated during cell differentiation, and the PIWI protein is required to regain control. Absence of SMEDWI-2 leads to tissue-specific transposon derepression related to cell-type-specific chromatin remodeling events and in addition causes reduced accessibility of lineage-specific genes and defective cell differentiation, resulting in fatal tissue dysfunction. Finally, we show that additional PIWI proteins provide a stem-cell-specific second layer of protection in planarian neoblasts. These findings reveal a far-reaching role of PIWI proteins and PIWI-interacting RNAs (piRNAs) in stem cell biology and cell differentiation.</p>',
'date' => '2021-10-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34610311',
'doi' => '10.1016/j.celrep.2021.109776',
'modified' => '2023-08-01 14:21:10',
'created' => '2023-08-01 15:59:38',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '214',
'name' => 'pA-Tn5 Transposase - unloaded SDS GB en',
'language' => 'en',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-GB-en-GHS_1_0.pdf',
'countries' => 'GB',
'modified' => '2020-06-08 16:13:25',
'created' => '2020-06-08 16:13:25',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '216',
'name' => 'pA-Tn5 Transposase - unloaded SDS US en',
'language' => 'en',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-US-en-GHS_1_0.pdf',
'countries' => 'US',
'modified' => '2020-06-08 16:15:02',
'created' => '2020-06-08 16:15:02',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '211',
'name' => 'pA-Tn5 Transposase - unloaded SDS DE de',
'language' => 'de',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded--DE-de-GHS_1_0.pdf',
'countries' => 'DE',
'modified' => '2020-06-08 16:11:36',
'created' => '2020-06-08 16:11:36',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '215',
'name' => 'pA-Tn5 Transposase - unloaded SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-JP-ja-GHS_2_0.pdf',
'countries' => 'JP',
'modified' => '2020-06-08 16:14:15',
'created' => '2020-06-08 16:14:15',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '210',
'name' => 'pA-Tn5 Transposase - unloaded SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-BE-nl-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2020-06-08 16:10:56',
'created' => '2020-06-08 16:10:56',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '213',
'name' => 'pA-Tn5 Transposase - unloaded SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-FR-fr-GHS_1_0.pdf',
'countries' => 'FR',
'modified' => '2020-06-08 16:12:46',
'created' => '2020-06-08 16:12:46',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '209',
'name' => 'pA-Tn5 Transposase - unloaded SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-BE-fr-GHS_1_0.pdf',
'countries' => 'BE',
'modified' => '2020-06-08 16:10:09',
'created' => '2020-06-08 16:10:09',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '212',
'name' => 'pA-Tn5 Transposase - unloaded SDS ES es',
'language' => 'es',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2020-06-08 16:12:16',
'created' => '2020-06-08 16:12:16',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$meta_canonical = 'https://www.diagenode.com/jp/p/pa-tn5-transposase-unloaded'
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
(int) 0 => array(
'id' => '3065',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µl',
'catalog_number' => 'C01070002',
'old_catalog_number' => '',
'sf_code' => 'C01070002-19650',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '650',
'price_USD' => '650',
'price_GBP' => '575',
'price_JPY' => '/',
'price_CNY' => '/',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded',
'meta_title' => 'pA-Tn5 Transposase (unloaded) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:36:57',
'created' => '2020-04-28 16:21:20'
)
)
$pro = array(
'id' => '3067',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '',
'label1' => 'Validation data',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '30 µl',
'catalog_number' => 'C01070002-30',
'old_catalog_number' => '',
'sf_code' => 'C01070002-143301',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '1155',
'price_USD' => '1155',
'price_GBP' => '1010',
'price_JPY' => '/',
'price_CNY' => '0',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded-30-ul',
'meta_title' => 'pA-Tn5 Transposase (unloaded)| Diagenode.com',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:37:23',
'created' => '2020-04-28 16:56:21',
'ProductsGroup' => array(
'id' => '312',
'product_id' => '3067',
'group_id' => '275'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '3065',
'antibody_id' => null,
'name' => 'pA-Tn5 Transposase - unloaded',
'description' => '<p><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> <img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/banners/banner-cutruncuttag-small.png" /></a></p>
<p><strong>pA-Tn5 Transposase</strong> is a fusion protein of hyperactive Tn5 transposase and protein A developed for the <strong>CUT&Tag assay</strong>. For flexibility, the fusion protein is not pre-loaded with sequencing adapters. Please follow the recommended <a href="https://www.diagenode.com/en/documents/assembly-protocol-patn5-20042020-rms" target="_blank">assembly protocol</a> prior to use in CUT&Tag or similar assays (ACT-seq, CoBATCH, TIP-seq and other).</p>
<p><strong>pA-Tn5 Transposase</strong></p>
<ul style="list-style-type: circle;">
<li>High enrichment and specificity of the signal around the TSS</li>
<li>No contamination<span style="font-weight: 400;"> with </span><i><span style="font-weight: 400;">E. coli</span></i><span style="font-weight: 400;"> DNA</span></li>
<li><span style="font-weight: 400;">Flexible versions: <a href="https://www.diagenode.com/en/products/view/3064">loaded</a> or unloaded</span></li>
<li><span style="font-weight: 400;">Excellent for CUT&Tag and similar assays (eg. TIP-seq)<br /></span></li>
</ul>
<p>As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">high performance in CUT&Tag</a>.</p>
<p class="lead"><strong>Check out all products recommended for CUT&Tag assay</strong>:</p>
<ul class="nobullet" style="list-style-type: circle;">
<li><a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&Tag kit for Histones</a> <span>- compatible with histome marks and some transcription factors</span></li>
<li>pA/Tn5 Transposase (<a href="https://www.diagenode.com/en/products/view/3064" target="_blank">loaded </a>or unloaded)</li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&Tag grade</a> and <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade</a> antibodies</li>
<li>Negative & positive CUT&Tag controls: <span>Antibody Package for CUT&Tag: <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">anti-rabbit</a> and <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">anti-mouse</a></span></li>
<li>DNA purification: <a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2</a> or <a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></li>
<li>Sequencing indexes: <a href="https://www.diagenode.com/en/p/24-si-for-tagmented-libraries">single indexes</a> and <a href="https://www.diagenode.com/en/p/24-unique-dual-indexes-for-tagmented-libraries-set1">UDI</a></li>
</ul>',
'label1' => 'Validation data',
'info1' => '<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">A.<br /><center><img alt="pA-Tn5 Transposase" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-a.jpg" width="600" height="265" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">B.<br /><center><img alt="pA-Tn5 Transposase unloaded" src="https://www.diagenode.com/img/product/cutandtag/C01070002-fig-b.jpg" /></center></div>
</div>
<div class="row extra-spaced">
<div class="small-12 medium-12 large-12 columns">
<p><strong>Figure 1: Quality control of pA-Tn5 transposase loaded with sequencing adapters</strong></p>
<p>A: The Fragment Analyzer trace showing the representative cleavage pattern of gDNA. The pA-Tn5 fusion protein (Cat. No. C01070002) loaded with sequencing adapters efficiently digests gDNA to a smear. 500 ng of human genomic DNA were incubated for 7 min at 55°C with 1 μl of pATn5 fusion protein loaded with appropriated adaptors in a tagmentation buffer (40mM Tris-HCl pH7.5, 40mM MgCl2 and 12.5% DMF). The reaction was stopped by adding SDS, cleaned-up and resolved on the Fragment Analyzer to assess the cleavage.</p>
<p>B: Representative screenshot at selected locus obtained using Diagenode pA-Tn5 fusion protein (Cat. No. C01070002) lloaded with sequencing adapters and H3K27me3 polyclonal ChIP-seq grade antibody (Cat. No. <a href="https://www.diagenode.com/en/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">C15410195</a>) following CUT&Tag protocol (Kaya-Okur, H.S., Nat Commun 10, 1930 (2019)).</p>
</div>
</div>',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '10 µl',
'catalog_number' => 'C01070002',
'old_catalog_number' => '',
'sf_code' => 'C01070002-19650',
'type' => 'FRE',
'search_order' => '',
'price_EUR' => '650',
'price_USD' => '650',
'price_GBP' => '575',
'price_JPY' => '/',
'price_CNY' => '/',
'price_AUD' => '/',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '',
'slug' => 'pa-tn5-transposase-unloaded',
'meta_title' => 'pA-Tn5 Transposase (unloaded) | Diagenode',
'meta_keywords' => '',
'meta_description' => 'pA-Tn5 Transposase (unloaded) is a fusion protein of hyperactive Tn5 transposase and protein A. For flexibility, the fusion protein is not pre-loaded with sequencing adapters.',
'modified' => '2024-08-29 09:36:57',
'created' => '2020-04-28 16:21:20'
)
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
'id' => '1077',
'name' => 'Transposome Assembly Using Diagenode pA-Tn5 Transposase',
'description' => '<p>pA-Tn5 transposase (Cat. No. C01070002) is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For flexibility of use, the fusion protein is not pre-loaded with sequencing adapters. The fusion protein should be loaded with appropriate oligonucleotides prior to use. Oligonucleotides should contain 19-mer Tn5 mosaic ends (underlined) recognized by the transposase and the sequences (bold) allowing the PCR amplification with Illumina-compatible barcoded i7/i5 primers. These sequences have to be adapted to a particular experimental design and take into account the sequencing platform requirements.</p>',
'image_id' => '246',
'type' => 'Protocol',
'url' => 'files/products/cutandtag/assembly-protocol-pAtn5-20042020-rms.pdf',
'slug' => 'assembly-protocol-patn5-20042020-rms',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2020-04-28 16:32:44',
'created' => '2020-04-28 16:32:44',
'ProductsDocument' => array(
'id' => '2878',
'product_id' => '3067',
'document_id' => '1077'
)
)
$sds = array(
'id' => '212',
'name' => 'pA-Tn5 Transposase - unloaded SDS ES es',
'language' => 'es',
'url' => 'files/SDS/pA-Tn5_Transposase/SDS-C01070002-pA-Tn5_Transposase_-_unloaded-ES-es-GHS_1_0.pdf',
'countries' => 'ES',
'modified' => '2020-06-08 16:12:16',
'created' => '2020-06-08 16:12:16',
'ProductsSafetySheet' => array(
'id' => '408',
'product_id' => '3067',
'safety_sheet_id' => '212'
)
)
$publication = array(
'id' => '4831',
'name' => 'PIWI-mediated control of tissue-specific transposons is essential for somatic cell differentiation',
'authors' => 'Danyan Li, David H Taylor, Josien C van Wolfswinkel',
'description' => '<p>PIWI proteins are known as mediators of transposon silencing in animal germlines but are also found in adult pluripotent stem cells of highly regenerative animals, where they are essential for regeneration. Study of the nuclear PIWI protein SMEDWI-2 in the planarian somatic stem cell system reveals an intricate interplay between transposons and cell differentiation in which a subset of transposons is inevitably activated during cell differentiation, and the PIWI protein is required to regain control. Absence of SMEDWI-2 leads to tissue-specific transposon derepression related to cell-type-specific chromatin remodeling events and in addition causes reduced accessibility of lineage-specific genes and defective cell differentiation, resulting in fatal tissue dysfunction. Finally, we show that additional PIWI proteins provide a stem-cell-specific second layer of protection in planarian neoblasts. These findings reveal a far-reaching role of PIWI proteins and PIWI-interacting RNAs (piRNAs) in stem cell biology and cell differentiation.</p>',
'date' => '2021-10-01',
'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34610311',
'doi' => '10.1016/j.celrep.2021.109776',
'modified' => '2023-08-01 14:21:10',
'created' => '2023-08-01 15:59:38',
'ProductsPublication' => array(
'id' => '7033',
'product_id' => '3067',
'publication_id' => '4831'
)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/34610311" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×
