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PHF8 polyclonal antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Catalog Number

Format

Price

C15410336

100 μl

$460.00
Bulk order

Other format

C15410336-20

20 μl

Polyclonal antibody raised in rabbit against human PHF8 (PHD finger protein 8), using a synthetic peptide containing a sequence from the central part of the protein.

1Manufactured by Bethyl Laboratories, Inc., Texas, USA

Lot	A301-772A4
Concentration	0.2 μg/μl
Species reactivity	Human
Type	Polyclonal ChIP grade
Purity	Affinity purified
Host	Rabbit
Storage Conditions	Store at 4°C
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP ^*	1-2 μg/ChIP	Fig 1
Western Blotting	1:1,000	Fig 2
IP	6 μg per IP	Fig 3

^* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

¹Manufactured by Bethyl Laboratories, Inc., Texas, USA

Validation data

Figure 1. ChIP results obtained with the Diagenode antibody directed against PHF8
ChIP assays were performed using HeLa cells, the Diagenode antibody against PHF8 (cat. No. C15410336) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 2 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8
Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. C15410336) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8
Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500.
Target Description
PHF8 (UniProtKB/Swiss-Prot entry Q9UPP1) is a jumonji domain containing protein which plays role in histone demethylation. PHF8 selectively demethylates the di- and monomethyl H3K9, H3K27 and H4K20 and acts as a transcriptional activator. It plays a key role cell cycle progression, rDNA transcription and brain development. Mutations in PHF8 lead to Siderius type X-linked mental retardation (MRXSSD), a mild to borderline type of mental retardation.

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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. C15410336) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 &mu;g of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500. </small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 &mu;g of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500. </small></p>
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<p><small><strong>Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 &mu;g of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 &mu;g of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500. </small></p>
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<p><small><strong>Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 &mu;g of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500. </small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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	'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
	'meta_title' => 'ChIP Quantitative PCR  Antibodies (ChIP-qPCR) | Diagenode',
	'modified' => '2016-01-20 11:30:24',
	'created' => '2015-10-20 11:45:36',
	'locale' => 'jpn'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
	'id' => '38',
	'name' => 'Epigenetic Antibodies Brochure',
	'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
	'image_id' => null,
	'type' => 'Brochure',
	'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
	'slug' => 'epigenetic-antibodies-brochure',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2016-06-15 11:24:06',
	'created' => '2015-07-03 16:05:27',
	'ProductsDocument' => array(
		'id' => '2290',
		'product_id' => '2866',
		'document_id' => '38'
	)
)
$sds = array(
	'id' => '3745',
	'name' => 'SDS C15410336 PHF8 Antibody DE de',
	'language' => 'de',
	'url' => 'files/SDS/PHF8/SDS-C15410336-PHF8_Antibody-DE-de-GHS_2_0.pdf',
	'countries' => 'DE',
	'modified' => '2024-01-17 18:51:23',
	'created' => '2024-01-17 18:51:23',
	'ProductsSafetySheet' => array(
		'id' => '6109',
		'product_id' => '2866',
		'safety_sheet_id' => '3745'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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PHF8 polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against human PHF8 (PHD finger protein 8), using a synthetic...	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

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PHF8 polyclonal antibody

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