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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against PHF8</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against PHF8 (cat. No. C15410336) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 2 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. C15410336) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. C15410336) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. C15410336) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against PHF8</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against PHF8 (cat. No. C15410336) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 2 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. C15410336) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against PHF8</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against PHF8 (cat. No. C15410336) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 2 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. C15410336) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against PHF8</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against PHF8 (cat. No. C15410336) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 2 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. C15410336) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against PHF8</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against PHF8 (cat. No. C15410336) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 2 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. C15410336) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. C15410336) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against PHF8</strong><br /> ChIP assays were performed using HeLa cells, the Diagenode antibody against PHF8 (cat. No. C15410336) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 2 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the EIF2S3 and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PHF8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against PHF8 (Cat. No. C15410336) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 3. Immunoprecipitation analysis using the Diagenode antibody directed against PHF8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against PHF8 (Cat. No. C15410336, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated PHF8 protein was detected by western blot with the PHF8 antibody diluted 1:500. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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'format' => '20 μl',
'catalog_number' => 'C15410336-20',
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'sf_code' => 'C15410336-361',
'type' => 'REF',
'search_order' => '03-Antibody',
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'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
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'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'phf8-polyclonal-antibody-20',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 18:52:11',
'created' => '2020-12-30 13:18:17',
'ProductsGroup' => array(
'id' => '379',
'product_id' => '3175',
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)
)
$pro = array(
'id' => '3175',
'antibody_id' => '636',
'name' => 'PHF8 Antibody (sample size)',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '20 μl',
'catalog_number' => 'C15410336-20',
'old_catalog_number' => '',
'sf_code' => 'C15410336-361',
'type' => 'REF',
'search_order' => '03-Antibody',
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'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
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'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'phf8-polyclonal-antibody-20',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 18:52:11',
'created' => '2020-12-30 13:18:17',
'ProductsGroup' => array(
'id' => '379',
'product_id' => '3175',
'group_id' => '338'
)
)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '3175',
'antibody_id' => '636',
'name' => 'PHF8 Antibody (sample size)',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '20 μl',
'catalog_number' => 'C15410336-20',
'old_catalog_number' => '',
'sf_code' => 'C15410336-361',
'type' => 'REF',
'search_order' => '03-Antibody',
'price_EUR' => '105',
'price_USD' => '115',
'price_GBP' => '100',
'price_JPY' => '16450',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'phf8-polyclonal-antibody-20',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 18:52:11',
'created' => '2020-12-30 13:18:17',
'ProductsGroup' => array(
'id' => '379',
'product_id' => '3175',
'group_id' => '338'
)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'ProductsApplication' => array(
'id' => '4557',
'product_id' => '2866',
'application_id' => '43'
)
)
$slugs = array(
(int) 0 => 'chip-qpcr-antibodies'
)
$applications = array(
'id' => '43',
'position' => '10',
'parent_id' => '40',
'name' => 'ChIP-qPCR (ab)',
'description' => '',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr-antibodies',
'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode',
'modified' => '2016-01-20 11:30:24',
'created' => '2015-10-20 11:45:36',
'locale' => 'zho'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
'id' => '38',
'name' => 'Epigenetic Antibodies Brochure',
'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
'image_id' => null,
'type' => 'Brochure',
'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf',
'slug' => 'epigenetic-antibodies-brochure',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-06-15 11:24:06',
'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
'id' => '2290',
'product_id' => '2866',
'document_id' => '38'
)
)
$sds = array(
'id' => '3745',
'name' => 'SDS C15410336 PHF8 Antibody DE de',
'language' => 'de',
'url' => 'files/SDS/PHF8/SDS-C15410336-PHF8_Antibody-DE-de-GHS_2_0.pdf',
'countries' => 'DE',
'modified' => '2024-01-17 18:51:23',
'created' => '2024-01-17 18:51:23',
'ProductsSafetySheet' => array(
'id' => '6109',
'product_id' => '2866',
'safety_sheet_id' => '3745'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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