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Alternative names: RBBP7/4, RBBP-7/4, RBAP46/48, NURF 55
Monoclonal antibody raised in mouse against the full length human RbAp48 protein (Retinoblastoma-binding protein p48) fused to GST. The antibody recognizes both RbAp48 and RbAp46.
| Lot | 001 |
|---|---|
| Concentration | 2.0 µg/µl |
| Species reactivity | Human |
| Type | Monoclonal |
| Purity | Protein G purified |
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Western Blotting | 1:1,000 | Fig 1 |
Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against hRbAp46/48
Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 μg) and the Diagenode monoclonal antibody against hRbAp46/48 (cat# MAb-033-050) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left), the position of the protein of interest is shown on the right.
| Datasheet hRbAp46-48 MAb-033-050 DATASHEET Datasheet description | Download |
| SDS C15200033 RbAp 46/48 Antibody GB en | Download |
| RbAp 46/48 Antibody SDS US en | Download |
| SDS C15200033 RbAp 46/48 Antibody US en | Download |
| RbAp 46/48 Antibody SDS GB en | Download |
| RbAp 46/48 Antibody SDS BE fr | Download |
| RbAp 46/48 Antibody SDS FR fr | Download |
| SDS C15200033 RbAp 46/48 Antibody BE nl | Download |
| RbAp 46/48 Antibody SDS ES es | Download |
| SDS C15200033 RbAp 46/48 Antibody FR fr | Download |
| RbAp 46/48 Antibody SDS DE de | Download |
| RbAp 46/48 Antibody SDS JP ja | Download |
| SDS C15200033 RbAp 46/48 Antibody ES es | Download |
| RbAp 46/48 Antibody SDS BE nl | Download |
| SDS C15200033 RbAp 46/48 Antibody DE de | Download |
| SDS C15200033 RbAp 46/48 Antibody JP ja | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: RbAp 46/48 monoclonal antibody (Diagenode Cat# C15200033 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'RbAp 46/48 monoclonal antibody - Pioneer', 'meta_title' => 'RbAp 46/48 monoclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '1974', 'antibody_id' => '52', 'name' => 'RbAp 46/48 monoclonal antibody ', 'description' => '<p>Alternative names: <span><strong>RBBP7/4</strong>, <strong>RBBP-7/4</strong>, <strong>RBAP46/48</strong>, <strong>NURF 55</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the full length human RbAp48 protein (Retinoblastoma-binding protein p48) fused to GST. The antibody recognizes both RbAp48 and RbAp46.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200033-fig1.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="237" width="97" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against hRbAp46/48</strong> <br />Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 μg) and the Diagenode monoclonal antibody against hRbAp46/48 (cat# MAb-033-050) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. 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The antibody recognizes both RbAp48 and RbAp46.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200033-fig1.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="237" width="97" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against hRbAp46/48</strong> <br />Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 μg) and the Diagenode monoclonal antibody against hRbAp46/48 (cat# MAb-033-050) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'RbAp 46/48 monoclonal antibody - Pioneer', 'meta_title' => 'RbAp 46/48 monoclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '1974', 'antibody_id' => '52', 'name' => 'RbAp 46/48 monoclonal antibody ', 'description' => '<p>Alternative names: <span><strong>RBBP7/4</strong>, <strong>RBBP-7/4</strong>, <strong>RBAP46/48</strong>, <strong>NURF 55</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the full length human RbAp48 protein (Retinoblastoma-binding protein p48) fused to GST. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'RbAp 46/48 monoclonal antibody - Pioneer', 'meta_title' => 'RbAp 46/48 monoclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '1974', 'antibody_id' => '52', 'name' => 'RbAp 46/48 monoclonal antibody ', 'description' => '<p>Alternative names: <span><strong>RBBP7/4</strong>, <strong>RBBP-7/4</strong>, <strong>RBAP46/48</strong>, <strong>NURF 55</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the full length human RbAp48 protein (Retinoblastoma-binding protein p48) fused to GST. The antibody recognizes both RbAp48 and RbAp46.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200033-fig1.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="237" width="97" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against hRbAp46/48</strong> <br />Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 μg) and the Diagenode monoclonal antibody against hRbAp46/48 (cat# MAb-033-050) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'RbAp 46/48 monoclonal antibody - Pioneer', 'meta_title' => 'RbAp 46/48 monoclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '1974', 'antibody_id' => '52', 'name' => 'RbAp 46/48 monoclonal antibody ', 'description' => '<p>Alternative names: <span><strong>RBBP7/4</strong>, <strong>RBBP-7/4</strong>, <strong>RBAP46/48</strong>, <strong>NURF 55</strong></span></p> <p><span>Monoclonal antibody raised in mouse against the full length human RbAp48 protein (Retinoblastoma-binding protein p48) fused to GST. The antibody recognizes both RbAp48 and RbAp46.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200033-fig1.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="237" width="97" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against hRbAp46/48</strong> <br />Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 μg) and the Diagenode monoclonal antibody against hRbAp46/48 (cat# MAb-033-050) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. 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The antibody recognizes both RbAp48 and RbAp46.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200033-fig1.jpg" alt="Western blot" style="display: block; margin-left: auto; margin-right: auto;" height="237" width="97" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against hRbAp46/48</strong> <br />Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 μg) and the Diagenode monoclonal antibody against hRbAp46/48 (cat# MAb-033-050) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. 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These include (1) the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; (2) the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; (3) the nucleosome remodelling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; (4) the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and (5) the NURF (nucleosome remodeling factor) complex. Therefore it may target chromatin assembly factors as well as chromatin remodeling factors and histone deacetylases to their histone substrates in a nucleosomal DNA dependent manner.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '2.0 µg/µl', 'reactivity' => 'Human', 'type' => 'Monoclonal', 'purity' => 'Protein G purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 1</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'locale' => 'jpn' ) $description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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