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<p><small><strong> Figure 1. RING1B antibody Immunofluorescence results</strong><br /> Immunofluorescence of RING1B antibody. Tissue: human HeLa cells. Fixation in methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Primary antibody at 1:300 for 20 minutes at 25°C. Secondary antibody: Alexa Fluor®488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain. </small></p>
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<p><small><strong> Figure 2. RING1B antibody western blot results</strong><br /> Western blot using the Diagenode RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 μg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of RING1B antibody incubated at room temperature. </small></p>
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<p><small><strong> Figure 2. RING1B antibody western blot results</strong><br /> Western blot using the Diagenode RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 μg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of RING1B antibody incubated at room temperature. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><small><strong> Figure 2. RING1B antibody western blot results</strong><br /> Western blot using the Diagenode RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 μg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of RING1B antibody incubated at room temperature. </small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
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<p><small><strong> Figure 2. RING1B antibody western blot results</strong><br /> Western blot using the Diagenode RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 μg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of RING1B antibody incubated at room temperature. </small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p><small><strong> Figure 1. RING1B antibody Immunofluorescence results</strong><br /> Immunofluorescence of RING1B antibody. Tissue: human HeLa cells. Fixation in methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Primary antibody at 1:300 for 20 minutes at 25°C. Secondary antibody: Alexa Fluor®488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain. </small></p>
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<p><small><strong> Figure 2. RING1B antibody western blot results</strong><br /> Western blot using the Diagenode RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 μg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of RING1B antibody incubated at room temperature. </small></p>
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'description' => 'RING1B (also known as BAP1, DING, Polycomb-M33 interacting protein Ring1B, Ring finger protein 1b, Ring finger protein 2 and RNF2) is one of the PcG proteins. The polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), a ubiquitin-conjugating enzyme that possesses ubiquitin ligase activity.',
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<p><small><strong> Figure 1. RING1B antibody Immunofluorescence results</strong><br /> Immunofluorescence of RING1B antibody. Tissue: human HeLa cells. Fixation in methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Primary antibody at 1:300 for 20 minutes at 25°C. Secondary antibody: Alexa Fluor®488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain. </small></p>
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<p><small><strong> Figure 2. RING1B antibody western blot results</strong><br /> Western blot using the Diagenode RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 μg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of RING1B antibody incubated at room temperature. </small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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