RRBS Service includes:
QC of the genomic DNA |
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Preparation of RRBS libraries |
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Deep sequencing |
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メチル化アレイとWGBS技術の長所を有するReduced representation bisulfite sequencing (RRBS) 技術は、あらゆる脊椎動物種で柔軟に使用できるため、大規模コホートの研究においても正確で費用対効果の高いアッセイを行えます。制限MspI酵素(CCGGターゲットサイト)を使用してゲノムを切断し、次にサイズを選択することで、濃縮されたDNAはプロモーターやCpGアイランドを含む関連するターゲットCpGリッチ領域を表します。またRRBS技術はゲノムの一部分を濃縮してメチル化解析を行うことによってシーケンス量を減らす手法でカバレッジが非常に高く、CpGメチル化解析に適しています。(ヒトサンプルで最大700万CpGが検出)。
TESTIMONIALWe are very happy with the services provided by Diagenode. In our project, Reduced Representation Bisulfite Sequencing (RRBS) was used and it worked well for both high-quality DNA and DNA extracted from formalin-fixed, paraffin-embedded (FFPE) material. Bioinformatic analyses were comprehensive and high-quality results were obtained. Diagenode’s services can be recommended.
Satu Mäki-Nevala, PhD, University of Helsinki
QC of the genomic DNA |
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Preparation of RRBS libraries |
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Deep sequencing |
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Analysis |
Features |
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Standard |
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Differential methylation analysis |
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Gene ontology terms analysis |
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Pathway analysis |
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We have had an excellent experience with the RRBS service provided by Diagenode. Our project was based on DNA extracted from human fresh and paraffin-embedded skeletal muscle, and the RRBS and Bioinformatic results were reliable and consistent with what we expécted. We highly recommend their service
Mario Roque - Instituto de Histología y Embriología de Mendoza (IHEM), Mendoza, Argentina
The new Diagenode Premium RRBS Kit makes it easy to use RRBS cost-effectively and with high throughput, using early sample pooling and multiplex sequencing. Most importantly, the method provides an improved coverage of up to 4 million CpGs for the human genome. We successfully used this protocol on more than 1,000 samples comprising of six different species, various cancers, FFPE and lowinput samples.
Paul Datlinger and Christoph Bock, CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria
Our laboratory has used RRBS sevice of Diagenode on murine and human samples. The service was impeccable in each phase, from the sample preparation to bionformatic analysis because it was always customer-oriented. I highly recommend my colleagues to use the RRBS service from Diagenode.
Prof. Lucia Altucci, MD, PhD, Seconda Università degli Studi di Napoli, Dipartimento di Biochimica, Biofisica e Patologia generale .
Diagenode Premium RRBS technology: Cost-effective DNA methylation mapping with superior CpG resolution and coverage APPLICATION NOTE DNA methylation is an important epigenetic mark with broad relevance in development and disease. ... | Download |
The Diagenode Epigenetics custom service POSTER Complete workflows for genome-scale DNA methylation and histone marks analysis Epigenetics is cr... | Download |
![]() How to properly cite our product/service in your workWe strongly recommend using this: RRBS受託サービス (Reduced Representation Bisulfite Sequencing) (Hologic Diagenode Cat# G02020000). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
Mission SpaceX CRS-19 RRRM-1 space flight induced skin genomic plasticity via an epigenetic trigger |
Long-term effects of myo-inositol on traumatic brain injury: Epigenomic and transcriptomic studies |
Gestational Caloric Restriction Alters Adipose Tissue Methylome and Offspring’s Metabolic Profile in a Swine Model |
Diagnostic Algorithm to Subclassify Atypical Spitzoid Tumors in Low and High Risk According to Their Methylation Status |
Sperm DNA methylation is predominantly stable in mice offspring bornafter transplantation of long-term cultured spermatogonial stem cells. |
Myelodysplastic Syndrome associated TET2 mutations affect NK cellfunction and genome methylation. |
Epigenetics and stroke: role of DNA methylation and effect of aging onblood-brain barrier recovery. |
Altered DNA methylation in estrogen-responsive repetitive sequences ofspermatozoa of infertile men with shortened anogenital distance. |
Epigenetic Alterations of Repeated Relapses in Patient-matchedChildhood Ependymomas. |
DNA methylation may affect beef tenderness through signal transduction inBos indicus. |
Genome-Wide Epigenomic Analyses in Patients With Nociceptive and Neuropathic Chronic Pain Subtypes Reveals Alterations in Methylation of Genes Involved in the Neuro-Musculoskeletal System |
Genome-wide epigenomic analyses in patients with nociceptive andneuropathic chronic pain subtypes reveals alterations in methylation ofgenes involved in the neuro-musculoskeletal system. |
Environmental enrichment preserves a young DNA methylation landscape in the aged mouse hippocampus |
Pathophysiological adaptations of resistance arteries in rat offspringexposed in utero to maternal obesity is associated with sex-specificepigenetic alterations. |
Muscle allele-specific expression QTLs may affect meat quality traitsin Bos indicus. |
IGFBP2 protects against pulmonary fibrosis through inhibiting P21-mediated senescence |
Perturbed DNA methylation by sustained overexpression of Gadd45b induces chromatin disorganization, DNA strand breaks and dopaminergic neurondeath in mice |
Developmental cannabidiol exposure increases anxiety and modifiesgenome-wide brain DNA methylation in adult female mice. |
Hepatic transcriptome and DNA methylation patterns following perinataland chronic BPS exposure in male mice. |
Integrative Analysis of Glucometabolic Traits, Adipose Tissue DNA Methylation and Gene Expression Identifies Epigenetic Regulatory Mechanisms of Insulin Resistance and Obesity in African Americans |
Chronic cannabidiol alters genome-wide DNA methylation in adult mouse hippocampus: epigenetic implications for psychiatric disease. |
LY75 Ablation Mediates Mesenchymal-Epithelial Transition (MET) in Epithelial Ovarian Cancer (EOC) Cells Associated with DNA Methylation Alterations and Suppression of the Wnt/β-Catenin Pathway. |
Obesogen effect of bisphenol S alters mRNA expression and DNA methylation profiling in male mouse liver |
Demethylation of ITGAV accelerates osteogenic differentiation in a blast-induced heterotopic ossification in vitro cell culture model. |
Paternal sepsis induces alterations of the sperm methylome and dampens offspring immune responses—an animal study |
DNMT3B overexpression contributes to aberrant DNA methylation and MYC-driven tumor maintenance in T-ALL and Burkitt’s lymphoma |
Diagenode® Premium RRBS technology: cost-effective DNA methylation mapping with superior coverage |
You are about to request a quote for データマイニングサービス. Fill out the form below and we will be in touch with you very soon.
All * fields are mandatory
You are about to request a quote for our epigenomics services. Fill out the form below and we will be in touch with you very soon.
All * fields are mandatory
You are about to request a quote for メチル化データ解析. Fill out the form below and we will be in touch with you very soon.
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