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Polyclonal antibody raised in rabbit against SMAD1, using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 1.31 μg/μl |
| Species reactivity | Human, mouse |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP/ChIP-seq * | 2-5 μg/ChIP | Fig 1, 2 |
| Western Blotting | 1:500 - 1:3,000 | Fig 3 |
| Immunoprecipitation | 2 μg/IP |
* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.
Figure 1. ChIP results obtained with the Diagenode antibody directed against SMAD1(S206p)
ChIP assays were performed using K562 cells, the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SMAD1(S206p)
ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274). The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human X chromosome (fig 2A and B) and in two genomic regions surrounding the GAPDH and EIF4A2 genes on chromosome 12 and 3, respectively (fig 2C and D).
Figure 3. Western blot analysis using the Diagenode antibody directed against SMAD1(S206p)
Whole cell extracts (15 μg) from HepG2 (lane 1) and HaCat (lane 2) cells were analysed by Western blot using the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) diluted 1:500.
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-seq (ab) Read more |
| ChIP-qPCR (ab) Read more |
| Datasheet SMAD1 C15410274 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: SMAD1 polyclonal antibody (Diagenode Cat# C15410274 Lot# 001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'SMAD1 polyclonal antibody - Classic', 'meta_title' => 'SMAD1 polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2393', 'antibody_id' => '420', 'name' => 'SMAD1 polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against SMAD1, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chip.png" alt="SMAD1 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-7 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-A.png" alt="SMAD1 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-B.png" alt="SMAD1 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-C.png" alt="SMAD1 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-D.png" alt="SMAD1 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-5 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274). The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human X chromosome (fig 2A and B) and in two genomic regions surrounding the GAPDH and EIF4A2 genes on chromosome 12 and 3, respectively (fig 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-WB-fig3.png" alt="SMAD1 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMAD1(S206p)</strong><br /> Whole cell extracts (15 μg) from HepG2 (lane 1) and HaCat (lane 2) cells were analysed by Western blot using the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) diluted 1:500. </small></p> </div> </div>', 'label2' => '', 'info2' => '<p>SMAD1 (UniProt/Swiss-Prot entry Q15797) is a transcriptional modulator that mediates multiple signaling pathways, such as those of the bone morphogenetic proteins (BMPs). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD1 can be phosphorylated by the BMP receptor kinase, leading to its activation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μg', 'catalog_number' => 'C15410274', 'old_catalog_number' => '', 'sf_code' => 'C15410274-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'smad1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'SMAD1 polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'SMAD1 polyclonal antibody - Classic', 'modified' => '2022-01-05 14:56:42', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '420', 'name' => 'SMAD1 polyclonal antibody - Classic', 'description' => 'SMAD1 (UniProt/Swiss-Prot entry Q15797) is a transcriptional modulator that mediates multiple signaling pathways, such as those of the bone morphogenetic proteins (BMPs). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD1 can be phosphorylated by the BMP receptor kinase, leading to its activation.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.31 μg/μl', 'reactivity' => 'Human, mouse', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2-5 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2 μg/IP</td> <td></td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. 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ChIP results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-7 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-A.png" alt="SMAD1 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-B.png" alt="SMAD1 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-C.png" alt="SMAD1 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-D.png" alt="SMAD1 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-5 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274). The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '18', 'position' => '20', 'parent_id' => '4', 'name' => 'Chromatin associated proteins', 'description' => '<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '521', 'name' => 'Datasheet SMAD1 C15410274', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_SMAD1_C15410274.pdf', 'slug' => 'datasheet-smad1-c15410274', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'SMAD1 polyclonal antibody - Classic', 'meta_title' => 'SMAD1 polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2393', 'antibody_id' => '420', 'name' => 'SMAD1 polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against SMAD1, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chip.png" alt="SMAD1 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-7 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-A.png" alt="SMAD1 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-B.png" alt="SMAD1 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-C.png" alt="SMAD1 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-D.png" alt="SMAD1 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-5 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274). The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human X chromosome (fig 2A and B) and in two genomic regions surrounding the GAPDH and EIF4A2 genes on chromosome 12 and 3, respectively (fig 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-WB-fig3.png" alt="SMAD1 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMAD1(S206p)</strong><br /> Whole cell extracts (15 μg) from HepG2 (lane 1) and HaCat (lane 2) cells were analysed by Western blot using the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) diluted 1:500. </small></p> </div> </div>', 'label2' => '', 'info2' => '<p>SMAD1 (UniProt/Swiss-Prot entry Q15797) is a transcriptional modulator that mediates multiple signaling pathways, such as those of the bone morphogenetic proteins (BMPs). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD1 can be phosphorylated by the BMP receptor kinase, leading to its activation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μg', 'catalog_number' => 'C15410274', 'old_catalog_number' => '', 'sf_code' => 'C15410274-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'smad1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'SMAD1 polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'SMAD1 polyclonal antibody - Classic', 'modified' => '2022-01-05 14:56:42', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '420', 'name' => 'SMAD1 polyclonal antibody - Classic', 'description' => 'SMAD1 (UniProt/Swiss-Prot entry Q15797) is a transcriptional modulator that mediates multiple signaling pathways, such as those of the bone morphogenetic proteins (BMPs). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD1 can be phosphorylated by the BMP receptor kinase, leading to its activation.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.31 μg/μl', 'reactivity' => 'Human, mouse', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2-5 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2 μg/IP</td> <td></td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:27:26', 'created' => '2015-07-28 11:56:21', 'select_label' => '420 - SMAD1 polyclonal antibody - Classic (001 - 1.31 μg/μl - Human, mouse - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'SMAD1 polyclonal antibody - Classic' $meta_title = 'SMAD1 polyclonal antibody - Classic' $product = array( 'Product' => array( 'id' => '2393', 'antibody_id' => '420', 'name' => 'SMAD1 polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against SMAD1, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chip.png" alt="SMAD1 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-7 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-A.png" alt="SMAD1 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-B.png" alt="SMAD1 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-C.png" alt="SMAD1 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-D.png" alt="SMAD1 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-5 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274). The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human X chromosome (fig 2A and B) and in two genomic regions surrounding the GAPDH and EIF4A2 genes on chromosome 12 and 3, respectively (fig 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-WB-fig3.png" alt="SMAD1 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMAD1(S206p)</strong><br /> Whole cell extracts (15 μg) from HepG2 (lane 1) and HaCat (lane 2) cells were analysed by Western blot using the Diagenode antibody against SMAD1(S206p) (Cat. 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SMAD1 can be phosphorylated by the BMP receptor kinase, leading to its activation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μg', 'catalog_number' => 'C15410274', 'old_catalog_number' => '', 'sf_code' => 'C15410274-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'smad1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'SMAD1 polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'SMAD1 polyclonal antibody - Classic', 'modified' => '2022-01-05 14:56:42', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '420', 'name' => 'SMAD1 polyclonal antibody - Classic', 'description' => 'SMAD1 (UniProt/Swiss-Prot entry Q15797) is a transcriptional modulator that mediates multiple signaling pathways, such as those of the bone morphogenetic proteins (BMPs). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD1 can be phosphorylated by the BMP receptor kinase, leading to its activation.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.31 μg/μl', 'reactivity' => 'Human, mouse', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2-5 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2 μg/IP</td> <td></td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:27:26', 'created' => '2015-07-28 11:56:21', 'select_label' => '420 - SMAD1 polyclonal antibody - Classic (001 - 1.31 μg/μl - Human, mouse - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '18', 'position' => '20', 'parent_id' => '4', 'name' => 'Chromatin associated proteins', 'description' => '<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. 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Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '521', 'name' => 'Datasheet SMAD1 C15410274', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_SMAD1_C15410274.pdf', 'slug' => 'datasheet-smad1-c15410274', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'SMAD1 polyclonal antibody - Classic', 'meta_title' => 'SMAD1 polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2393', 'antibody_id' => '420', 'name' => 'SMAD1 polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against SMAD1, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chip.png" alt="SMAD1 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-7 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-A.png" alt="SMAD1 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-B.png" alt="SMAD1 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-C.png" alt="SMAD1 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-D.png" alt="SMAD1 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-5 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274). The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human X chromosome (fig 2A and B) and in two genomic regions surrounding the GAPDH and EIF4A2 genes on chromosome 12 and 3, respectively (fig 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-WB-fig3.png" alt="SMAD1 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMAD1(S206p)</strong><br /> Whole cell extracts (15 μg) from HepG2 (lane 1) and HaCat (lane 2) cells were analysed by Western blot using the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) diluted 1:500. </small></p> </div> </div>', 'label2' => '', 'info2' => '<p>SMAD1 (UniProt/Swiss-Prot entry Q15797) is a transcriptional modulator that mediates multiple signaling pathways, such as those of the bone morphogenetic proteins (BMPs). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD1 can be phosphorylated by the BMP receptor kinase, leading to its activation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μg', 'catalog_number' => 'C15410274', 'old_catalog_number' => '', 'sf_code' => 'C15410274-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'smad1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'SMAD1 polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'SMAD1 polyclonal antibody - Classic', 'modified' => '2022-01-05 14:56:42', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '420', 'name' => 'SMAD1 polyclonal antibody - Classic', 'description' => 'SMAD1 (UniProt/Swiss-Prot entry Q15797) is a transcriptional modulator that mediates multiple signaling pathways, such as those of the bone morphogenetic proteins (BMPs). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD1 can be phosphorylated by the BMP receptor kinase, leading to its activation.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.31 μg/μl', 'reactivity' => 'Human, mouse', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2-5 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2 μg/IP</td> <td></td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:27:26', 'created' => '2015-07-28 11:56:21', 'select_label' => '420 - SMAD1 polyclonal antibody - Classic (001 - 1.31 μg/μl - Human, mouse - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'SMAD1 polyclonal antibody - Classic' $meta_title = 'SMAD1 polyclonal antibody - Classic' $product = array( 'Product' => array( 'id' => '2393', 'antibody_id' => '420', 'name' => 'SMAD1 polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against SMAD1, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chip.png" alt="SMAD1 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-7 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-A.png" alt="SMAD1 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-B.png" alt="SMAD1 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-C.png" alt="SMAD1 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-D.png" alt="SMAD1 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-5 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274). The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human X chromosome (fig 2A and B) and in two genomic regions surrounding the GAPDH and EIF4A2 genes on chromosome 12 and 3, respectively (fig 2C and D). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-WB-fig3.png" alt="SMAD1 Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMAD1(S206p)</strong><br /> Whole cell extracts (15 μg) from HepG2 (lane 1) and HaCat (lane 2) cells were analysed by Western blot using the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) diluted 1:500. </small></p> </div> </div>', 'label2' => '', 'info2' => '<p>SMAD1 (UniProt/Swiss-Prot entry Q15797) is a transcriptional modulator that mediates multiple signaling pathways, such as those of the bone morphogenetic proteins (BMPs). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD1 can be phosphorylated by the BMP receptor kinase, leading to its activation.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μg', 'catalog_number' => 'C15410274', 'old_catalog_number' => '', 'sf_code' => 'C15410274-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'smad1-polyclonal-antibody-classic-100-mg', 'meta_title' => 'SMAD1 polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'SMAD1 polyclonal antibody - Classic', 'modified' => '2022-01-05 14:56:42', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '420', 'name' => 'SMAD1 polyclonal antibody - Classic', 'description' => 'SMAD1 (UniProt/Swiss-Prot entry Q15797) is a transcriptional modulator that mediates multiple signaling pathways, such as those of the bone morphogenetic proteins (BMPs). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD1 can be phosphorylated by the BMP receptor kinase, leading to its activation.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '1.31 μg/μl', 'reactivity' => 'Human, mouse', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2-5 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:500 - 1:3,000</td> <td>Fig 3</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2 μg/IP</td> <td></td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:27:26', 'created' => '2015-07-28 11:56:21', 'select_label' => '420 - SMAD1 polyclonal antibody - Classic (001 - 1.31 μg/μl - Human, mouse - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '18', 'position' => '20', 'parent_id' => '4', 'name' => 'Chromatin associated proteins', 'description' => '<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '521', 'name' => 'Datasheet SMAD1 C15410274', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_SMAD1_C15410274.pdf', 'slug' => 'datasheet-smad1-c15410274', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'SMAD1 polyclonal antibody - Classic', 'meta_title' => 'SMAD1 polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2393', 'antibody_id' => '420', 'name' => 'SMAD1 polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against SMAD1, using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chip.png" alt="SMAD1 Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-7 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-A.png" alt="SMAD1 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-B.png" alt="SMAD1 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-C.png" alt="SMAD1 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-D.png" alt="SMAD1 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-5 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274). The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. 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ChIP results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. 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ChIP-seq results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274). The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. 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These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '521', 'name' => 'Datasheet SMAD1 C15410274', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_SMAD1_C15410274.pdf', 'slug' => 'datasheet-smad1-c15410274', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. 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