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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274). The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human X chromosome (fig 2A and B) and in two genomic regions surrounding the GAPDH and EIF4A2 genes on chromosome 12 and 3, respectively (fig 2C and D). </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMAD1(S206p)</strong><br /> Whole cell extracts (15 μg) from HepG2 (lane 1) and HaCat (lane 2) cells were analysed by Western blot using the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) diluted 1:500. </small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-B.png" alt="SMAD1 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-C.png" alt="SMAD1 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-D.png" alt="SMAD1 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274). The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human X chromosome (fig 2A and B) and in two genomic regions surrounding the GAPDH and EIF4A2 genes on chromosome 12 and 3, respectively (fig 2C and D). </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMAD1(S206p)</strong><br /> Whole cell extracts (15 μg) from HepG2 (lane 1) and HaCat (lane 2) cells were analysed by Western blot using the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) diluted 1:500. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMAD1(S206p)</strong><br /> Whole cell extracts (15 μg) from HepG2 (lane 1) and HaCat (lane 2) cells were analysed by Western blot using the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) diluted 1:500. </small></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274). The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human X chromosome (fig 2A and B) and in two genomic regions surrounding the GAPDH and EIF4A2 genes on chromosome 12 and 3, respectively (fig 2C and D). </small></p>
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<td>2-5 μg/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500 - 1:3,000</td>
<td>Fig 3</td>
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<tr>
<td>Immunoprecipitation</td>
<td>2 μg/IP</td>
<td></td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>',
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-A.png" alt="SMAD1 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-B.png" alt="SMAD1 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410274-chipseq-C.png" alt="SMAD1 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274). The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human X chromosome (fig 2A and B) and in two genomic regions surrounding the GAPDH and EIF4A2 genes on chromosome 12 and 3, respectively (fig 2C and D). </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMAD1(S206p)</strong><br /> Whole cell extracts (15 μg) from HepG2 (lane 1) and HaCat (lane 2) cells were analysed by Western blot using the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) diluted 1:500. </small></p>
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<p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SMAD1(S206p)</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 5 μg of the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274). The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human X chromosome (fig 2A and B) and in two genomic regions surrounding the GAPDH and EIF4A2 genes on chromosome 12 and 3, respectively (fig 2C and D). </small></p>
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<p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SMAD1(S206p)</strong><br /> Whole cell extracts (15 μg) from HepG2 (lane 1) and HaCat (lane 2) cells were analysed by Western blot using the Diagenode antibody against SMAD1(S206p) (Cat. No. C15410274) diluted 1:500. </small></p>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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