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WDR5 polyclonal antibody

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Catalog Number

Format

Price

C15310101
(CS-101-100)

100 µl

$260.00
Bulk order

Alternative names: BIG3, SWD3

Polyclonal antibody raised in rabbit against mouse Wdr5 (WD (tryptophan-aspartate) repeat domain protein 5), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.

Lot	A266-004
Concentration	not determined
Species reactivity	Mouse
Type	Polyclonal
Purity	Whole antiserum
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:100	Fig 1
Western Blotting	1:1,000	Fig 2, (1)

Validation Data

Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Wdr5 (Cat. No. CS-101-100). By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:12,300.

Figure 2. Western blot analysis using the Diagenode antibody directed against Wdr5 (1)
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Wdr5 (Cat. No. CS-101-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (36 kDa) is indicated on the right.

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Chd8 regulates X chromosome inactivation in mouse through fine-tuningcontrol of Xist expression.
Cerase, Andrea et al.
Female mammals achieve dosage compensation by inactivating one of their two X chromosomes during development, a process entirely dependent on Xist, an X-linked long non-coding RNA (lncRNA). At the onset of X chromosome inactivation (XCI), Xist is up-regulated and spreads along the future inactive X chromosome. Conte...

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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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<th>References</th>
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<td>ELISA</td>
<td>1:100</td>
<td>Fig 1</td>
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<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 2, (1)</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><span>Polyclonal antibody raised in rabbit against mouse Wdr5 (WD (tryptophan-aspartate) repeat domain protein 5), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.</span></p>',
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<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Wdr5 (Cat. No. CS-101-100). By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:12,300. </small></p>
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<p class="text-center"><img src="http://www.diagenode.com/img/product/antibodies/C15310101_fig2.jpg" alt="Western blot" height="175" width="132" /></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Wdr5 (1)</strong><br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Wdr5 (Cat. No. CS-101-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (36 kDa) is indicated on the right.</small></p>
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<th>References</th>
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<td>1:100</td>
<td>Fig 1</td>
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<div class="small-6 columns">
<p class="text-center"><img src="http://www.diagenode.com/img/product/antibodies/C15310101_fig1.jpg" alt="ELISA" height="298" width="372" /></p>
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<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Wdr5 (Cat. No. CS-101-100). By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:12,300. </small></p>
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<div class="small-6 columns">
<p class="text-center"><img src="http://www.diagenode.com/img/product/antibodies/C15310101_fig2.jpg" alt="Western blot" height="175" width="132" /></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Wdr5 (1)</strong><br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Wdr5 (Cat. No. CS-101-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (36 kDa) is indicated on the right.</small></p>
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<td>1:100</td>
<td>Fig 1</td>
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<td>Fig 2, (1)</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
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	'authors' => 'Cerase, Andrea et al.',
	'description' => '<p>Female mammals achieve dosage compensation by inactivating one of their two X chromosomes during development, a process entirely dependent on Xist, an X-linked long non-coding RNA (lncRNA). At the onset of X chromosome inactivation (XCI), Xist is up-regulated and spreads along the future inactive X chromosome. Contextually, it recruits repressive histone and DNA modifiers that transcriptionally silence the X chromosome. Xist regulation is tightly coupled to differentiation and its expression is under the control of both pluripotency and epigenetic factors. Recent evidence has suggested that chromatin remodelers accumulate at the X Inactivation Center (XIC) and here we demonstrate a new role for Chd8 in Xist regulation in differentiating ES cells, linked to its control and prevention of spurious transcription factor interactions occurring within Xist regulatory regions. Our findings have a broader relevance, in the context of complex, developmentally-regulated gene expression.</p>',
	'date' => '2021-04-01',
	'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33859315',
	'doi' => '10.1038/s42003-021-01945-1',
	'modified' => '2021-12-21 16:17:34',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Wdr5 (Cat. No. CS-101-100). By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:12,300. </small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Wdr5 (1)</strong><br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Wdr5 (Cat. No. CS-101-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (36 kDa) is indicated on the right.</small></p>
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<td>Fig 1</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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Datasheet Wdr5 CS-101-100 DATASHEET Datasheet description	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

SDS C15310101 WDR5 Antibody GB en	Download
SDS C15310101 WDR5 Antibody US en	Download
SDS C15310101 WDR5 Antibody DE de	Download
SDS C15310101 WDR5 Antibody JP ja	Download
SDS C15310101 WDR5 Antibody BE nl	Download
SDS C15310101 WDR5 Antibody BE fr	Download
SDS C15310101 WDR5 Antibody FR fr	Download
SDS C15310101 WDR5 Antibody ES es	Download