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WDR5 Antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

Antibody portal

Antibodies

Catalog Number

Format

Price

C15310101
(CS-101-100)

100 µl

$260.00
Bulk order

Alternative names: BIG3, SWD3

Polyclonal antibody raised in rabbit against mouse Wdr5 (WD (tryptophan-aspartate) repeat domain protein 5), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.

Lot	A266-004
Concentration	not determined
Species reactivity	Mouse
Type	Polyclonal
Purity	Whole antiserum
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:100	Fig 1
Western Blotting	1:1,000	Fig 2, (1)

Validation Data

Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Wdr5 (Cat. No. CS-101-100). By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:12,300.

Figure 2. Western blot analysis using the Diagenode antibody directed against Wdr5 (1)
Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Wdr5 (Cat. No. CS-101-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (36 kDa) is indicated on the right.

Publications

How to properly cite this product in your work

Diagenode strongly recommends using this: WDR5 Antibody (Diagenode Cat# C15310101 Lot# A266-004 ). Click here to copy to clipboard.

Using our products in your publication? Let us know!

Chd8 regulates X chromosome inactivation in mouse through fine-tuningcontrol of Xist expression.
Cerase, Andrea et al.
Female mammals achieve dosage compensation by inactivating one of their two X chromosomes during development, a process entirely dependent on Xist, an X-linked long non-coding RNA (lncRNA). At the onset of X chromosome inactivation (XCI), Xist is up-regulated and spreads along the future inactive X chromosome. Conte...

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<th>References</th>
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<td>ELISA</td>
<td>1:100</td>
<td>Fig 1</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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	'doi' => '10.1038/s42003-021-01945-1',
	'modified' => '2021-12-21 16:17:34',
	'created' => '2021-12-06 15:53:19',
	'ProductsPublication' => array(
		'id' => '5372',
		'product_id' => '2092',
		'publication_id' => '4176'
	)
)
$externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/33859315" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
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	'meta_description' => 'WDR5 (WD (tryptophan-aspartate)  repeat domain protein 5) Polyclonal Antibody  validated  in  WB and ELISA. Batch-specific data available on the website. Alternative names: BIG3, SWD3',
	'meta_title' => 'WDR5 Polyclonal Antibody | Diagenode',
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			'name' => 'WDR5 Antibody ',
			'description' => '<p><span>Alternative names: <strong>BIG3</strong>, <strong>SWD3</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against mouse Wdr5 (WD (tryptophan-aspartate) repeat domain protein 5), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.</span></p>',
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			'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="http://www.diagenode.com/img/product/antibodies/C15310101_fig1.jpg" alt="ELISA" height="298" width="372" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Wdr5 (Cat. No. CS-101-100). By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:12,300. </small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="http://www.diagenode.com/img/product/antibodies/C15310101_fig2.jpg" alt="Western blot" height="175" width="132" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Wdr5 (1)</strong><br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Wdr5 (Cat. No. CS-101-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (36 kDa) is indicated on the right.</small></p>
</div>
</div>',
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			'isotype' => '',
			'lot' => 'A266-004 ',
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			'classification' => 'Pioneer',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:100</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
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<td>Fig 2, (1)</td>
</tr>
</tbody>
</table>',
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			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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$meta_description = 'WDR5 (WD (tryptophan-aspartate)  repeat domain protein 5) Polyclonal Antibody  validated  in  WB and ELISA. Batch-specific data available on the website. Alternative names: BIG3, SWD3'
$meta_title = 'WDR5 Polyclonal Antibody | Diagenode'
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		'id' => '2092',
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		'name' => 'WDR5 Antibody ',
		'description' => '<p><span>Alternative names: <strong>BIG3</strong>, <strong>SWD3</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against mouse Wdr5 (WD (tryptophan-aspartate) repeat domain protein 5), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein.</span></p>',
		'label1' => 'Validation Data',
		'info1' => '<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="http://www.diagenode.com/img/product/antibodies/C15310101_fig1.jpg" alt="ELISA" height="298" width="372" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Wdr5 (Cat. No. CS-101-100). By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:12,300. </small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="http://www.diagenode.com/img/product/antibodies/C15310101_fig2.jpg" alt="Western blot" height="175" width="132" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Wdr5 (1)</strong><br />Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the Diagenode antibody against mouse Wdr5 (Cat. No. CS-101-100), diluted 1:1,000 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (36 kDa) is indicated on the right.</small></p>
</div>
</div>',
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		'info2' => '',
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		'info3' => '',
		'format' => '100 µl',
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		'price_EUR' => '260',
		'price_USD' => '260',
		'price_GBP' => '245',
		'price_JPY' => '40730',
		'price_CNY' => '',
		'price_AUD' => '650',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => true,
		'last_datasheet_update' => '0000-00-00',
		'slug' => 'wdr5-polyclonal-antibody-pioneer-100-ul-1',
		'meta_title' => 'WDR5 Polyclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'WDR5 (WD (tryptophan-aspartate)  repeat domain protein 5) Polyclonal Antibody  validated  in  WB and ELISA. Batch-specific data available on the website. Alternative names: BIG3, SWD3',
		'modified' => '2024-01-11 16:24:00',
		'created' => '2015-06-29 14:08:20',
		'locale' => 'eng'
	),
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		'name' => 'WDR5 polyclonal antibody ',
		'description' => 'Wdr5 (UniProtKB/Swiss-Prot entry P61964) belongs to the family of WD repeat proteins which are involved in different cellular processes such as cell cycle progression, signal transduction, apoptosis, and gene regulation. It is a component of the Set1A and Set1B histone H3 methylation complexes. These complexes methylate lysine 4 of H3, thereby activating gene transcription. Wdr5 interacts with H3 dimethyl K4, but not with tri or mono methylated H3K4.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A266-004 ',
		'concentration' => 'not determined',
		'reactivity' => 'Mouse',
		'type' => 'Polyclonal',
		'purity' => 'Whole antiserum',
		'classification' => 'Pioneer',
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:100</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 2, (1)</td>
</tr>
</tbody>
</table>',
		'storage_conditions' => '',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
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		'modified' => '2016-08-03 15:14:04',
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			'name' => 'ELISA',
			'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
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			'in_footer' => false,
			'in_menu' => false,
			'online' => true,
			'tabular' => true,
			'slug' => 'elisa-antibodies',
			'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody',
			'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications',
			'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
			'modified' => '2016-01-13 12:21:41',
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			)
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			'id' => '19',
			'position' => '10',
			'parent_id' => '40',
			'name' => 'WB',
			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
			'in_footer' => false,
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			'online' => true,
			'tabular' => true,
			'slug' => 'western-blot-antibodies',
			'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
			'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
			'modified' => '2016-04-26 12:44:51',
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			'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
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			'cookies_tag_id' => null,
			'meta_keywords' => 'monoclonal antibodies,policlonal antibodies,Histone modifying enzymes',
			'meta_description' => 'Diagenode Offers Polyclonal and Monoclonal Antibodies Against Histone Modifying Enzymes like: Histone Deacetylases, Histone Demethylases, Histone Transferases.',
			'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode',
			'modified' => '2019-07-04 16:19:15',
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		(int) 1 => array(
			'id' => '103',
			'position' => '0',
			'parent_id' => '4',
			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'meta_description' => null,
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		),
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			'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
			'image_id' => null,
			'type' => 'Poster',
			'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf',
			'slug' => 'antibodies-you-can-trust-poster',
			'meta_keywords' => '',
			'meta_description' => '',
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			'image_id' => null,
			'type' => 'Brochure',
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			'meta_description' => '',
			'modified' => '2016-06-15 11:24:06',
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			'name' => 'product/antibodies/antibody.png',
			'alt' => 'Mouse IgG',
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			'name' => 'Chd8 regulates X chromosome inactivation in mouse through fine-tuningcontrol of Xist expression.',
			'authors' => 'Cerase, Andrea et al.',
			'description' => '<p>Female mammals achieve dosage compensation by inactivating one of their two X chromosomes during development, a process entirely dependent on Xist, an X-linked long non-coding RNA (lncRNA). At the onset of X chromosome inactivation (XCI), Xist is up-regulated and spreads along the future inactive X chromosome. Contextually, it recruits repressive histone and DNA modifiers that transcriptionally silence the X chromosome. Xist regulation is tightly coupled to differentiation and its expression is under the control of both pluripotency and epigenetic factors. Recent evidence has suggested that chromatin remodelers accumulate at the X Inactivation Center (XIC) and here we demonstrate a new role for Chd8 in Xist regulation in differentiating ES cells, linked to its control and prevention of spurious transcription factor interactions occurring within Xist regulatory regions. Our findings have a broader relevance, in the context of complex, developmentally-regulated gene expression.</p>',
			'date' => '2021-04-01',
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			'doi' => '10.1038/s42003-021-01945-1',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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	'authors' => 'Cerase, Andrea et al.',
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	'date' => '2021-04-01',
	'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33859315',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<th>References</th>
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<td>ELISA</td>
<td>1:100</td>
<td>Fig 1</td>
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<td>1:1,000</td>
<td>Fig 2, (1)</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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Datasheet Wdr5 CS-101-100 DATASHEET Datasheet description	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

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