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• Download Activity Test - Procedure for immunoprecipitation of native chromatin proteins
  Proteins play role in almost all cellular processes, and the study of their activity is crucial for understanding these mechanisms. Slimane AIT-SI-ALI and his “epigenetics and cell fate&rdquo...
• Download ChIP - Just 4 steps to foolproofing fragmentation for ChIP using Bioruptor^® Standard, Plus or Pico
  Just follow these four steps for shearing chromatin, and you’ll be well on your way to excellent ChIP results without the troubleshooting headache.
• Download ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus
  A review of the critical points for chromatin shearing.
• Download ChIP from plant material with the LowCell# ChIP Kit
  As preparation of plant material for Chromatin Immunoprecipiation differs essentially from preparation of culture cells, the LowCell# ChIP protocol has to be adapted respectively. We recommend ther...
• Download Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor^® Pico
  Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in cells and tissues. The assay can be cumbersome and the success is highly dependent on the quality of ...
• Download Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor^® Standard, Plus or Pico
  This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq ki...
• Download Chromatin Shearing in Yeast Cells with Bioruptor® Standard and Plus
  Standard procedure to shear Chromatin from Yeast. 
• Download Chromatin shearing with the Bioruptor Pico 10 µl - 300 µl
  HeLa cells are fixed with 1% molecular grade formaldehyde (for 8 min at room temperature). Nucleus isolation is performed using buffers and reagents of Diagenode’s Chromatin Shearing Optimiza...
• Download Chromatin shearing with the Bioruptor^® Pico 500 µl - 2 ml
  Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShear kit - Low SDS (previous name: Chromatin Shearing Optimization kit – Low SDS) protocol (Cat. ...
• Download Chromatin shearing with the Bioruptor® Pico, 0.2 ml Bioruptor® Microtubes and the corresponding holder
  The first critical step of a successful ChIP experiment is the preparation of sheared chromatin with a suitable fragment range which best represents the biological scenario being investigated. The ...
• Download TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®
  The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein complex. The fusion of the TAP tag to the protein of interest allows the rapid purification under a nat...
• Download Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®
  The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using Bioruptor&...