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Chromatin studies

Chromatin immunoprecipitation (ChIP) is a method used to determine the location of genome binding sites for a specific protein of interest, giving invaluable insights into the regulation of gene expression. ChIP involved the selective enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognize a specific protein or protein modification are used to determine the relative abundance of the antigen at specific loci. ChIP-seq and ChIP-qPCR are techniques that allow the identification of protein-DNA binding sites.

The ChIP workflow

Step by step workflow	Optimal solution from Diagenode

1. Crosslink to bind proteins to DNA	ChIP Cross-link Gold

2. Shear DNA	Bioruptor^® Pico Sonication device Shearing optimization reagent

3. Immunoprecipitate with specific antibody	ChIP-seq and ChIP-qPCR kits for transcription factors, histones, low inputs, plants ChIP and ChIP-seq grade antibodies Automation available

4. Reverse crosslinks and purify	Diagenode's ChIP kits (contain optimal purification modules)

5. ChIP-qPCR or ChIP-seq library preparation	ChIP-seq: MicroPlex Library Preparation for 50 pg - 5 ng iDeal Library Preparation for > 5 ng ChIP qPCR

Products for chromatin study

Crosslinking
Efficient solution for protein-protein fixation. Read more

Chromatin shearing
Perfectly sheared chromatin is critical for ChIP success.
Read more about reagent optimization.
Read more about Bioruptor sonication.

Chromatin immunoprecipitation
Immunoprecipitation solutions for histone and transcription factor ChIP-qPCR and ChIP-seq for low inputs, plants, and animals including automated solutions. Read more

ChIPmentation
ChIPmentation, an exclusive technology, is an end-to-end ChIP-seq solution for low and difficult inputs. Read more

ChIP and ChIP-seq antibodies
ChIP-grade antibodies are essential for success. Learn more

Primer pairs
Highly specific primer pairs for the amplification of the specific genomic regions. Read more

DNA purification
Read more about solutions for DNA purification

Library preparation for ChIP-seq
Optimized solutions for the library preparation from low DNA input. Read more

ChIP and ChIP-seq automation
Reproducibility, optimization simplicity, no variability. Learn more

Chromatin assembly products
Study the nucleosome environment. Learn more

Chromatin resources

Posters

Application notes

Publications related to ChIP

Brochures

产品

目录号	产品	格式	价格
C01080002	ATAC-seq kit	24 rxns	$1,745.00

目录号	产品	格式	价格
C01011011	µChIPmentation for Histones The Diagenode µChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries. T...	24 rxns	$2,400.00
C01011032	SI for Tagmented libraries The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. The primer indexes of the 24 SI Tagmented libraries ar...	24 SI	$185.00
C01011000	Auto ChIPmentation Kit for Histones Contact us This product must be used with the IP-Star Compact Automated System. Diagenode’s latest technology for histone ChIP-seq, ...	24 rxns
C01011010	ChIPmentation Kit for Histones Contact us Difficult and challenging histone ChIP-seq is now solved. Diagenode’s latest technology for histone ChIP-seq, ChIPmentation ...	24 rxns
C01011030	TAG Kit for ChIPmentation Contact us Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fra...	24 rxns	$1,050.00