DNA modifications

The pattern of DNA modifications is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the DNA methyltransferases DNMT3A and B and DNMT1. DNMT3A and DNMT3B are responsible for de novo DNA methylation, whereas DNMT1 maintains existing methylation. 5-mC undergoes active demethylation which is performed by the Ten-Eleven Translocation (TET) familly of DNA hydroxylases. The latter consists of 3 members TET1, 2 and 3. All 3 members catalyze the conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine (5-hmC), and further into 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC). 5-fC and 5-caC can be converted to unmodified cytosine by Thymine DNA Glycosylase (TDG). It is not yet clear if 5-hmC, 5-fC and 5-caC have specific functions or are simply intermediates in the demethylation of 5-mC.

DNA methylation is generally considered as a repressive mark and is usually associated with gene silencing. It is essential that the balance between DNA methylation and demethylation is precisely maintained. Dysregulation of DNA methylation may lead to many different human diseases and is often observed in cancer cells.

Diagenode offers highly validated antibodies against different proteins involved in DNA modifications as well as against the modified bases allowing the study of all steps and intermediates in the DNA methylation/demethylation pathway:

Diagenode exclusively sources the original 5-methylcytosine monoclonal antibody (clone 33D3).

Check out the list below to see all proposed antibodies for DNA modifications.

Diagenode’s highly validated antibodies:

Highly sensitive and specific
Cost-effective (requires less antibody per reaction)
Batch-specific data is available on the website
Expert technical support
Sample sizes available
100% satisfaction guarantee

产品

目录号	Product	应用	纯度
C15200003	5-methylcytosine (5-mC) Antibody for ICC/IF (50 μg/39 μl)	DB FISH IF	Protein A purified monoclonal antibody.
C15410209	3-methylcytosine (3-mC) Antibody (50 μg/28 μl)	DB	Protein G purified
C15410204-100	5-Carboxylcytosine (5-caC) Antibody (100 µg)	DB IF IP	Affinity purified
C15310200	5-formylcytosine (5-fC) Antibody (100 µl)	DIP ELISA	Whole antiserum
C15200200	5-hydroxymethylcytosine (5-hmC) Antibody (mouse) (50 µg/50 µl)	DB ELISA	Protein G purified
C15410205	5-hydroxymethylcytosine (5-hmC) Antibody (rabbit) (50 μg)	DB ELISA Methylated DNA immunoprecipitation	Affinity purified
C15310210-100	5-hydroxymethylcytosine (5-hmC) Antibody (rabbit) (100 µl)	DB ELISA	Whole antiserum
C15220001	5-hydroxymethylcytosine (5-hmC) Antibody (rat) (50 µg)	DB ELISA	Affinity purified
C15200006-100	5-methylcytosine (5-mC) Antibody - cl. b (100 µg)	IF Methylated DNA immunoprecipitation	Monoclonal antibody purified by gel filtration.
C15200081-100	5-methylcytosine (5-mC) Antibody - clone 33D3 (100 µg)	DB IF Methylated DNA immunoprecipitation	Protein A purified monoclonal antibody.
C15310111	DNMT1 Antibody (100 µl)	ELISA WB	Whole antiserum
C15410084	DNMT3A Antibody (50 µg/67 µl)	ELISA IP WB	Affinity purified
C15410085	DNMT3A Antibody (50 µg/54 µl)	ELISA IP WB	Affinity purified
C15410086	DNMT3A Antibody (50 µg/64 µl)	ELISA IP WB	Affinity purified
C15410218	DNMT3B Antibody (50 μg/ 16 μl)	ELISA IF WB	Affinity purified
C15310044	DNMT3B Antibody (100 µl)	ELISA WB	Whole antiserum
C15200227	TDG Antibody (50 μg)	IF WB	Affinity purified
C15200179	TET2 Antibody (50 μg/50 μl)	IP WB	Protein G purified
C15410255-100	TET2 Antibody (100 μl)	ChIP-qPCR IF IP WB	Affinity purified
C15410311	TET3 Antibody (50 μg)	ELISA WB	Affinity purified

产品

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