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Since 2004 Diagenode has been the most trusted supplier for epigenetics-focused antibodies. We understand that high quality is crucial for the success of your ChIP and ChIP-seq experiments, so we emphasize strict quality standards and actual validation for all of our antibodies. Our partnerships with leading epigenetics experts and epigenetics consortiums such as BLUEPRINT have allowed us to validate our antibodies to the highest degree, guaranteeing specificity, reproducibility, and success for each and every ChIP assay. In addition, unlike competing suppliers, we are fully transparent with our validation and provide all batch-specific validation data for tested applications on every data sheet right here on our website.

Diagenode has epigenetics antibodies of choice:

• Unmatched expertise - We have been exclusively dedicated to epigenetics research with more than 10 years of epigenetics-focused antibody expertise
• Strict quality standards with rigorous QC and validation using standardized protocols
• Comprehensive selection of histone and non-histone antibodies
• Numerous validation steps to guarantee your results, including peptide array confirmation and siRNA validation to assure antibody specificity

ChIP-seq grade antibodies

ChIP grade antibodies

Western Blot Antibodies

Chromatin associated proteins

Histone modifying enzymes

Isotype controls

DNA modifications

RNA associated proteins

RNA modifications

Histone antibodies

CRISPR/Cas9 antibodies

CUT&Tag Antibodies

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			'description' => '<p>Since 2004 Diagenode has been the most trusted supplier for epigenetics-focused antibodies. We understand that high quality is crucial for the success of your <strong>ChIP</strong> and <strong>ChIP-seq</strong> experiments, so we emphasize strict quality standards and actual validation for all of our antibodies. Our partnerships with leading epigenetics experts and epigenetics consortiums such as BLUEPRINT have allowed us to validate our antibodies to the highest degree, guaranteeing specificity, reproducibility, and success for each and every <strong>ChIP</strong> assay. In addition, unlike competing suppliers, we are fully transparent with our validation and provide all batch-specific validation data for tested applications on every data sheet right here on our website.</p>
<p>Diagenode has epigenetics antibodies of choice:</p>
<ul>
<li><strong>Unmatched expertise</strong> - We have been exclusively dedicated to epigenetics research with more than 10 years of epigenetics-focused antibody expertise</li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation using standardized protocols</li>
<li><strong>Comprehensive</strong> selection of histone and non-histone antibodies</li>
<li><strong>Numerous validation steps to guarantee your results</strong>, including peptide array confirmation and siRNA validation to assure antibody specificity</li>
</ul>
<div class="row"><br />
<div class="small-12 medium-6 large-6 columns text-center"><a href="./chip-seq-grade-antibodies"><img width="105" height="105" alt="services chip-seq" src="https://www.diagenode.com/img/categories/antibodies/ChIP-seq-icon.png" /></a> <br /><br /> <a class="details button" href="./chip-seq-grade-antibodies">ChIP-seq grade antibodies</a></div>
<div class="small-12 medium-6 large-6 columns text-center"><a href="./chip-grade-antibodies"><img width="105" height="105" alt="services methylation" src="https://www.diagenode.com/img/categories/antibodies/ChIP-with-qPCR-icon.png" /></a> <br /><br /> <a class="details button" href="./chip-grade-antibodies">ChIP grade antibodies</a></div>
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<div class="row">
<div class="large-12 columns" style="text-align: justify;"><br />
<blockquote><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">TESTIMONIAL</span>
<p>I am working with Diagenode's antibodies for over 5 years now. The great and specific performance as well as the reliable high quality of the antibodies are crucial for the success of my ChIP experiments.</p>
<cite>Dr Holger Bierhoff, Division Molecular Biology of the Cell II, Prof. Dr. Ingrid Grummt&rsquo;s lab, DKFZ, Heidelberg, Germany</cite></blockquote>
<h3 class="text-center"><img width="105" height="105" alt="Epigenetics success guaranteed" src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" /><a href="../pages/antibodies-validation">&nbsp;&nbsp;Learn about Diagenode&rsquo;s antibody validation program </a></h3>
<hr />
<div class="row">
<div class="small-12 medium-6 large-6 columns text-center"><a href="../categories/all-antibodies" color="#13b29c">All Antibodies</a></div>
<div class="small-12 medium-6 large-6 columns text-center"><a href="../categories/sample-size-antibodies" color="#13b29c">Sample Size Antibodies</a></div>
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<h3>Antibody categories</h3>
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<p>Diagenode has epigenetics antibodies of choice:</p>
<ul>
<li><strong>Unmatched expertise</strong> - We have been exclusively dedicated to epigenetics research with more than 10 years of epigenetics-focused antibody expertise</li>
<li><strong>Strict quality standards</strong> with rigorous QC and validation using standardized protocols</li>
<li><strong>Comprehensive</strong> selection of histone and non-histone antibodies</li>
<li><strong>Numerous validation steps to guarantee your results</strong>, including peptide array confirmation and siRNA validation to assure antibody specificity</li>
</ul>
<div class="row"><br />
<div class="small-12 medium-6 large-6 columns text-center"><a href="./chip-seq-grade-antibodies"><img width="105" height="105" alt="services chip-seq" src="https://www.diagenode.com/img/categories/antibodies/ChIP-seq-icon.png" /></a> <br /><br /> <a class="details button" href="./chip-seq-grade-antibodies">ChIP-seq grade antibodies</a></div>
<div class="small-12 medium-6 large-6 columns text-center"><a href="./chip-grade-antibodies"><img width="105" height="105" alt="services methylation" src="https://www.diagenode.com/img/categories/antibodies/ChIP-with-qPCR-icon.png" /></a> <br /><br /> <a class="details button" href="./chip-grade-antibodies">ChIP grade antibodies</a></div>
</div>
<div class="row">
<div class="large-12 columns" style="text-align: justify;"><br />
<blockquote><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">TESTIMONIAL</span>
<p>I am working with Diagenode's antibodies for over 5 years now. The great and specific performance as well as the reliable high quality of the antibodies are crucial for the success of my ChIP experiments.</p>
<cite>Dr Holger Bierhoff, Division Molecular Biology of the Cell II, Prof. Dr. Ingrid Grummt&rsquo;s lab, DKFZ, Heidelberg, Germany</cite></blockquote>
<h3 class="text-center"><img width="105" height="105" alt="Epigenetics success guaranteed" src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" /><a href="../pages/antibodies-validation">&nbsp;&nbsp;Learn about Diagenode&rsquo;s antibody validation program </a></h3>
<hr />
<div class="row">
<div class="small-12 medium-6 large-6 columns text-center"><a href="../categories/all-antibodies" color="#13b29c">All Antibodies</a></div>
<div class="small-12 medium-6 large-6 columns text-center"><a href="../categories/sample-size-antibodies" color="#13b29c">Sample Size Antibodies</a></div>
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<hr />
<h3>Antibody categories</h3>
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
			'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'name' => 'Western Blot Antibodies',
			'description' => '<h2>We have what you need for a successful Western blot!</h2><p>Western blots require multiple steps - sample preparation, sample loading, electrophoresis, protein transfer, antibody incubation, and signal detection - so it is important to be sure that each step is done correctly.</p><p>For a successful Western blot we offer:</p><h3 class="diacol"><strong>Loading control</strong></h3><p>Use Diagenode&rsquo;s <strong>H3pan monoclonal antibody</strong> as a loading control for nuclear samples to compare the protein expression level between different samples.</p><p></p><div class="row"><div class="small-5 medium-5 large-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15200011-wb.jpg" /></div><div class="small-7 medium-7 large-7 columns"><small><strong>Western blot analysis using H3pan monoclonal antibody</strong> <br />Western blot was performed on whole cell extracts (30 &mu;g) from different cell types (lane 1: HeLa, lane 2: K562, lane 3: MCF7, lane 4: U2OS, lane 5: HepG2, lane 6: Jurkat, lane 7: NIH3T3, lane 8: E14Tg2a mouse ES cells) using the monoclonal antibody against H3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left,and the position of the protein of interest is indicated on the right.</small></div></div><p><strong></strong></p><p><strong>Why do you need a loading control?</strong></p><ul><li>To verify that equal sample amount was loaded on the gel</li><li>To confirm that the electrotransfer was done equally across the lines</li><li>To check that the signal detection after antibody incubation is homogenous across the lines</li></ul><p class="text-right"><a href="../p/h3pan-monoclonal-antibody-classic-50-mg-100-ml" class="details radius button">Learn more about this antibody <i class="fa fa-angle-double-right"></i></a></p><h3 class="diacol"><strong>Blue ladder-HRP monoclonal antibody</strong></h3><h3>Visualize your marker directly on film</h3><p>Most prestained protein MW markers used in western blot contain fragments that are labelled with a blue dye. Diagenode has&nbsp;developed a revolutionary new antibody that specifically reacts with this blue dye, enabling direct visualization of the different marker fragments on the blot. This makes the positioning of the marker and thus the accurate detection of proteins significantly easier.</p><ul><li>Faster and easier protein detection - manual marking is no longer necessary</li><li>Develop your marker directly on X-ray film</li><li>Reduced ladder consumption</li><li>Compatible with most blue-stained ladders</li><li>Outstanding specificity with no cross-reactivity or background</li></ul><div class="row"><div class="small-5 medium-5 large-5 columns text-center"><img src="https://www.diagenode.com/img/applications/blue_ladder_1.jpg" /></div><div class="small-7 medium-7 large-7 columns"><small><strong>Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong>&nbsp;<br />PAGE analysis of 5 different protein MW markers: 1. Page Ruler Plus (Thermo, cat. No. 26619); 2. Color Plus broad range (NEB, cat. No. P7711); 3. Page Ruler (Unstained, Fermentas, cat. No. SM0661); 4. Spectra Multicolor (Fermentas, cat. No. SM1859); 5. Precision Plus (Biorad, cat. No. 161-0373). 4 &mu;l of each marker was loaded on the gel. The right panel shows the Western blot analysis with the Diagenode Blue ladder - HRP antibody (cat. No. C1520202) diluted 1:1,000, using a standard Western blot protocol.</small></div></div><div class="row"><div class="small-12 columns "><br /><br /><br /></div></div><div class="row"><div class="small-5 medium-5 large-5 columns text-center"><img src="https://www.diagenode.com/img/applications/blue_ladder_2.jpg" /></div><div class="small-7 medium-7 large-7 columns"><small><strong>Western blot analysis using the Diagenode Blue ladder - HRP monoclonal antibody</strong> <br />Protein extracts from different species were subjected to SDS-PAGE and analyzed by Western blot with the Diagenode Blue ladder - HRP antibody (Cat. No. C15200202), diluted 1:1,000. The results are shown on the right. The left figure shows a Ponceau staining of the gel. This figure clearly demonstrates that the antibody does not react with any proteins of the species that were tested. </small></div></div><p>&nbsp;</p><p class="text-right"><a href="../p/blue-ladder-hrp-monoclonal-antibody-100-ul" class="details radius button">Learn more <i class="fa fa-angle-double-right"></i></a><br /> <span class="alert label">New!</span> <a href="../p/blue-ladder-monoclonal-antibody-50-&mu;l">Blue ladder monoclonal antibody</a> is available</p><h3 class="diacol"><strong>Antibodies validated in WB</strong></h3><p>Diagenode offers the antibodies selected for highest-quality results in Western blot. The <strong>high sensitivity</strong> and <strong>specificity</strong> of our antibodies enable the most accurate results.</p><blockquote><span class="label-green" style="margin-bottom: 16px; margin-left: -22px;">TESTIMONIAL</span><p><span>I have been a Diagenode customer for over two years now. I have used the antibodies against the histone modifications <strong>H3K4me3</strong>, <strong>H3K4me2</strong>, <strong>H3K4me1</strong>, <strong>H3k27me3</strong>, <strong>H3K9ac</strong>, <strong>H3K27ac</strong> etc. provided by Diagenode to perform <a href="../../applications/western-blot">western blots</a>.The high level of specificity of these antibodies in Pristionchus pacificus samples, confirmed by using several negative and positive controls run in parallel with synchronized culture samples, ensured successful and reproducible results.</span></p><p><span>Also, I am satisfied with the service and the support of personnel of Diagenode. They are prompt in replying to my emails and very helpful with their advice.</span>&nbsp;Thanks a lot!</p><cite>Vahan Serobyan, Prof. Dr Ralf J. Sommer's Lab, Max Planck Institute for Developmental Biology, T&uuml;bingen, Germany</cite></blockquote><div class="row"><div class="small-5 medium-5 large-5 columns text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15200229-WB-B.jpg" height="265" width="232" /></div><div class="small-7 medium-7 large-7 columns"><small><strong>Western blot analysis using the Diagenode antibody directed against CRISPR/Cas9 (Cat. No. C15200229)</strong><br /> Western blot was performed on protein extracts from HeLa cells transfected with Cas9 (lane 1) or from untransfected cells (lane 2) using the Diagenode antibody against CRISPR/Cas9, diluted 1:4,000 in PBS-T containing 3% NFDM. The marker is shown on the left, position of the Cas9 protein is indicated on the right.</small></div></div><div class="row"><div class="small-5 medium-5 large-5 columns text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410310-WB.png" /></div><div class="small-7 medium-7 large-7 columns"><small><strong>Western blot analysis using the Diagenode antibody directed against H3K14ac (cat. No. C15410310)</strong><br /> Western blot was performed on whole cell (25 &mu;g, lane 1) and histone extracts (15 &mu;g, lane 2) from HeLa cells, and on 1 &mu;g of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the Diagenode antibody against H3K14ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></div></div><p class="text-right"><a href="../applications/western-blot-antibodies" class="details radius button">Check out our selection! <i class="fa fa-angle-double-right"></i></a></p>',
			'no_promo' => false,
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			'slug' => 'western-blot-antibodies',
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			'meta_keywords' => 'Western Blot Antibodies',
			'meta_description' => 'Western Blot Antibodies',
			'meta_title' => 'Western Blot Antibodies',
			'modified' => '2019-07-03 11:00:28',
			'created' => '2019-07-03 11:00:28'
		),
		(int) 5 => array(
			'id' => '18',
			'position' => '20',
			'parent_id' => '4',
			'name' => 'Chromatin associated proteins',
			'description' => '<p><span style="font-weight: 400;">Diagenode&rsquo;s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
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			'online' => true,
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			'slug' => 'chromatin-modifying-and-or-associated-proteins',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody',
			'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins',
			'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode',
			'modified' => '2019-07-04 16:19:01',
			'created' => '2014-09-13 08:16:51'
		),
		(int) 6 => array(
			'id' => '21',
			'position' => '20',
			'parent_id' => '4',
			'name' => 'Histone modifying enzymes',
			'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
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			'slug' => 'chromatin-modifying-proteins-histone-transferase',
			'cookies_tag_id' => null,
			'meta_keywords' => 'monoclonal antibodies,policlonal antibodies,Histone modifying enzymes',
			'meta_description' => 'Diagenode Offers Polyclonal and Monoclonal Antibodies Against Histone Modifying Enzymes like: Histone Deacetylases, Histone Demethylases, Histone Transferases.',
			'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode',
			'modified' => '2019-07-04 16:19:15',
			'created' => '2014-06-26 13:18:41'
		),
		(int) 7 => array(
			'id' => '22',
			'position' => '40',
			'parent_id' => '4',
			'name' => 'Isotype controls',
			'description' => '<p><span style="font-weight: 400;">Diagenode offers the negative Ctrl IgG from rabbit, rat and mouse. &nbsp;These extensively validated antibodies can be used as negative controls in ChIP, IF, hMeDIP or other experiments performed with specific antibodies made in rabbit, rat or mouse, respectively.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
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			'is_antibody' => true,
			'slug' => 'isotype-controls',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Isotype controls,DNA immunoprecipitation,Methylated DNA immunoprecipitation',
			'meta_description' => 'Diagenode provides Isotype controls for Methylated DNA immunoprecipitation',
			'meta_title' => 'Isotype controls for Methylated DNA immunoprecipitation | Diagenode',
			'modified' => '2019-07-04 16:19:36',
			'created' => '2014-09-30 14:23:34'
		),
		(int) 8 => array(
			'id' => '23',
			'position' => '40',
			'parent_id' => '4',
			'name' => 'DNA modifications',
			'description' => '<p><span style="font-weight: 400;">T</span><span style="font-weight: 400;">he pattern of <strong>DNA modifications</strong> is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the <strong>DNA methyltransferases</strong> DNMT3A and B and DNMT1. DNMT3A and DNMT3B are responsible for </span><i><span style="font-weight: 400;">de novo</span></i><span style="font-weight: 400;"> DNA methylation, whereas DNMT1 maintains existing methylation. 5-mC undergoes active demethylation which is performed by the <strong>Ten-Eleven Translocation</strong> (TET) familly of DNA hydroxylases. The latter consists of 3 members TET1, 2 and 3. All 3 members catalyze the conversion of <strong>5-methylcytosine</strong> (5-mC) into <strong>5-hydroxymethylcytosine</strong> (5-hmC), and further into <strong>5-formylcytosine</strong> (5-fC) and <strong>5-carboxycytosine</strong> (5-caC). 5-fC and 5-caC can be converted to unmodified cytosine by <strong>Thymine DNA Glycosylase</strong> (TDG). It is not yet clear if 5-hmC, 5-fC and 5-caC have specific functions or are simply intermediates in the demethylation of 5-mC.</span></p>
<p><span style="font-weight: 400;">DNA methylation is generally considered as a repressive mark and is usually associated with gene silencing. It is essential that the balance between DNA methylation and demethylation is precisely maintained. Dysregulation of DNA methylation may lead to many different human diseases and is often observed in cancer cells.</span></p>
<p><span style="font-weight: 400;">Diagenode offers highly validated antibodies against different proteins involved in DNA modifications as well as against the modified bases allowing the study of all steps and intermediates in the DNA methylation/demethylation pathway:</span></p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/dna-methylation.jpg" height="599" width="816" /></p>
<p><strong>Diagenode exclusively sources the original 5-methylcytosine monoclonal antibody (clone 33D3).</strong></p>
<p>Check out the list below to see all proposed antibodies for DNA modifications.</p>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
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			'slug' => 'dna-methylation-antibody',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Monoclonal Antibodies,Polyclonal  antibodies,DNA methylation,Diagenode',
			'meta_description' => 'Diagenode offers Monoclonal and Polyclonal antibodies for DNA Methylation. The pattern of DNA modifications is critical for genome stability and the control of gene expression in the cell. ',
			'meta_title' => 'DNA modifications - Monoclonal and Polyclonal Antibodies for DNA Methylation | Diagenode',
			'modified' => '2020-08-03 15:25:37',
			'created' => '2014-07-31 03:51:53'
		),
		(int) 9 => array(
			'id' => '28',
			'position' => '40',
			'parent_id' => '4',
			'name' => 'RNA associated proteins',
			'description' => '<div class="row">
<div style="text-align: justify;" class="large-12 columns">
<p><span style="font-weight: 400;">Diagenode&rsquo;s large range of highly validated antibodies includes the antibodies to study RNA-associated proteins. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA-associated proteins studies below.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>
</div>
</div>
<p></p>',
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			'slug' => 'mirna-sirna',
			'cookies_tag_id' => null,
			'meta_keywords' => 'miRNA & siRNA meta  Polyclonal antibody,Monoclonal antibody',
			'meta_description' => 'Diagenode offers Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing',
			'meta_title' => 'Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing | Diagenode',
			'modified' => '2020-09-01 11:42:06',
			'created' => '2014-11-12 02:34:56'
		),
		(int) 10 => array(
			'id' => '109',
			'position' => '40',
			'parent_id' => '4',
			'name' => 'RNA modifications',
			'description' => '<div class="row">
<div style="text-align: justify;" class="large-12 columns">
<p><span style="font-weight: 400;">Diagenode&rsquo;s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>
</div>
</div>',
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			'slug' => 'rna-modifications-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody',
			'meta_description' => 'Diagenode offers a wide range of  RNA modifications antibodies',
			'meta_title' => 'RNA modifications antibodies | Diagenode.com',
			'modified' => '2020-09-01 11:43:28',
			'created' => '2016-02-15 10:58:08'
		),
		(int) 11 => array(
			'id' => '111',
			'position' => '40',
			'parent_id' => '4',
			'name' => 'Histone antibodies',
			'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p>
<p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome.&nbsp; They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include&nbsp; methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation.&nbsp; The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p>
<p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>&rdquo;, analogous to the genetic code.</p>
<p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p>
<p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our&nbsp; antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p>
<p>Diagenode&rsquo;s collection includes antibodies recognizing:</p>
<ul>
<li><strong>Histone H1 variants</strong></li>
<li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li>
<li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li>
<li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li>
<li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li>
</ul>
<p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p>
<p><span style="font-weight: 400;"> Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
</ul>',
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			'slug' => 'histone-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Histone, antibody, histone h1, histone h2, histone h3, histone h4',
			'meta_description' => 'Polyclonal and Monoclonal Antibodies against Histones and their modifications validated for many applications, including Chromatin Immunoprecipitation (ChIP) and ChIP-Sequencing (ChIP-seq)',
			'meta_title' => 'Histone and Modified Histone Antibodies | Diagenode',
			'modified' => '2020-09-17 13:34:56',
			'created' => '2016-04-01 16:01:32'
		),
		(int) 12 => array(
			'id' => '100',
			'position' => '41',
			'parent_id' => '4',
			'name' => 'CRISPR/Cas9 antibodies',
			'description' => '<p>The <strong>CRISPR/Cas genome editing system</strong> uses a RNA-guided endonuclease technology which allows for inducing indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, this technology, can also be used to mediate up- or downregulation of specific endogenous genes or to alter histone modifications or DNA methylation. The best characterized CRISPR-associated nucleases are the <strong>Cas9 proteins</strong> from <em>Streptococcus pyogenes</em> and <em>Staphylococcus aureus</em>. Recently two new CRISPR-associated nucleases, named <strong>Cpf1</strong> (from <em>Lachnospiraceae bacterium</em> and <em>Acidaminococcus sp.</em>), have been described.<br /><br /><span>Diagenode, a dedicated supplier of high quality Cas9 antibodies, was the first company that offered the antibody Cas9 (clone 7A9). </span><span>Diagenode now offers the largest collection of optimized anti-CRISPR/Cas antibodies, validated in a number of different applications including Western blot (WB), immunofluorescence (IF), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP).</span></p>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3><a name="4reasons"></a>Four critical reasons to use anti-CRISPR/Cas9 antibodies</h3>
<p>CRISPR/Cas9 genome editing allows for double-stranded DNA breaks at specific sequences to efficiently disrupt, excise, mutate, insert, or replace genes. The precision of transfection and the level of Cas9 expression should be controlled during the editing processes using specific anti-CRISPR/Cas9 antibodies.</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-1.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #1</strong> Check the transfection success of the Cas9 protein</h4>
<p>Check <strong>Cas9 expression level</strong> using <strong>Western blot</strong> with validated anti-Cas9 antibodies.</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-2.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #2</strong> Check that the Cas9 protein was delivered to the nucleus</h4>
<p>Determine the nuclear localization of Cas9 protein via <strong>IF</strong> or <strong>IHC</strong> using validated anti-Cas9 antibodies</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-3.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #3</strong> Check the Cas9 expression level</h4>
<p><strong>Transient systems</strong>: Check that <strong>Cas9 expression</strong> was transient by using <strong>Western blot</strong> and anti-Cas9 antibodies. Prolonged Cas9 expression will lead to more off-target mutations.</p>
<p><strong>Stable transformants</strong>: Isolate several clones and screen to check the level of <strong>Cas9 expression</strong> using <strong>Western blot</strong> and specific anti-Cas9 antibodies. High level of Cas9 expression can lead to non-specific activity.</p>
</div>
</div>
<div class="row">
<div class="small-2 medium-2 large-2 columns"><img src="https://www.diagenode.com/img/product/antibodies/cas9-reason-4.png" /></div>
<div class="small-10 medium-10 large-10 columns">
<h4><strong>Reason #4</strong> Check the binding specificity of Cas9</h4>
<p>Test the binding specificity with an sgRNA of choice by using the <strong>ChIP-grade anti-Cas9 antibody</strong> and the primers for targeted and non-targeted region to see if Cas9 is bound to the correct region.</p>
<p>For more details go to the page <strong><a href="https://www.diagenode.com/en/categories/selection-of-guide-rna-by-ChIP">Selection of guide RNA by ChIP</a></strong>.</p>
</div>
</div>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>Published examples of the use of anti-Cas9 antibodies:</h3>
<ul>
<li><a href="../publications/view/2823">A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing</a></li>
<li><a href="../publications/view/2792">A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage</a></li>
<li><a href="../publications/view/2765">Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection</a></li>
<li><a href="../publications/view/2679">Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells</a></li>
<li><a href="../publications/view/2645">Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis</a></li>
<li><a href="../publications/view/2961">An inducible lentiviral guide RNA platform enables the identification of tumor-essential genes and tumor-promoting mutations in vivo</a></li>
</ul>
<h5><strong>Diagenode offers the largest collection of validated anti-CRISPR/Cas antibodies, validated in different applications (WB, IF, IP and ChIP).</strong></h5>
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<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
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include - APP/View/Categories/view.ctp, line 244
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
CategoriesController::slug() - APP/Controller/CategoriesController.php, line 471
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Transcription

Epitope tags

Other antibodies