CRISPR-Cas9 Structures and Mechanisms Published: May, 2017 Abstract:
Many bacterial CRISPR-Cas systems employ the dual-RNA-guided DNA endonuclease Cas9 to defend against invading phages and conjugative plasmids by introducing site-specific double-stranded breaks in target DNA. Target recognition strictly requires t... Read more »
Osterix and RUNX2 are Transcriptional Regulators of Sclerostin in Human Bone Published: May, 2016 Abstract:
Sclerostin, encoded by the SOST gene, works as an inhibitor of the Wnt pathway and therefore is an important regulator of bone homeostasis. Due to its potent action as an inhibitor of bone formation, blocking sclerostin activity is the purpose of ... Read more »
saRNA-guided Ago2 targets the RITA complex to promoters to stimulate transcription Published: February, 2016 Abstract:
Small activating RNAs (saRNAs) targeting specific promoter regions are able to stimulate gene expression at the transcriptional level, a phenomenon known as RNA activation (RNAa). It is known that RNAa depends on Ago2 and is associated with epigen... Read more »
RecG Directs DNA Synthesis during Double-Strand Break Repair Published: February, 2016 Abstract:
Homologous recombination provides a mechanism of DNA double-strand break repair (DSBR) that requires an intact, homologous template for DNA synthesis. When DNA synthesis associated with DSBR is convergent, the broken DNA strands are replaced and r... Read more »
Analysis of EZH2: micro-RNA network in low and high grade astrocytic tumors Published: January, 2016 Abstract:
Enhancer of Zeste homologue2 (EZH2) is an epigenetic regulator that functions as oncogene in astrocytic tumors, however, EZH2 regulation remains little studied. In this study, we measured EZH2 levels in low (Gr-II,DA) and high grade (Gr-IV,GBM) as... Read more »
Transducin-like enhancer of split-1 is expressed and functional in human macrophages Published: December, 2015 Abstract:
Macrophages display heterogeneous phenotypes, including the classical M1 proinflammatory and the alternative M2 anti-inflammatory polarization states. The transducin-like enhancer of split-1 (TLE1) is a transcriptional corepressor whose functions ... Read more »
Standardizing chromatin research: a simple and universal method for ChIP-seq Published: December, 2015 Abstract:
Here we demonstrate that harmonization of ChIP-seq workflows across cell types and conditions is possible when obtaining chromatin from properly isolated nuclei. We established an ultrasound-based nuclei extraction method (Nuclei Extraction b... Read more »
Regulatory role of PAK1 and CtBP1 in muscle chromatin Published: September, 2015 Abstract:
Researchers in this article showed that the co-repressor CtBP1 is present on the myogenin promoter with repressive histone marks. They found that preventing CtBP1 Ser158 phosphorylation induces CtBP1 accumulation in the nuclei and abrogates the ac... Read more »
LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2 Published: August, 2015 Abstract:
High cholesterol and diabetes are major risk factors for atherosclerosis. Regression of atherosclerosis is mediated in part by the Liver X Receptor (LXR) through the induction of genes involved in cholesterol transport and efflux. In the context o... Read more »
