A LINE1-Nucleolin Partnership Regulates Early Development and ESC Identity. Published: July, 2018 Abstract:
Transposable elements represent nearly half of mammalian genomes and are generally described as parasites, or "junk DNA." The LINE1 retrotransposon is the most abundant class and is thought to be deleterious for cells, yet it is paradoxically high... Read more »
Epigenome profiling and editing of neocortical progenitor cells during development Published: September, 2017 Abstract:
The generation of neocortical neurons from neural progenitor cells (NPCs) is primarily controlled by transcription factors binding to DNA in the context of chromatin. To understand the complex layer of regulation that orchestrates different NPC ty... Read more »
Genomic Characterization of Metformin Hepatic Response Published: November, 2016 Abstract:
Metformin is used as a first-line therapy for type 2 diabetes (T2D) and prescribed for numerous other diseases. However, its mechanism of action in the liver has yet to be characterized in a systematic manner. To comprehensively identify genes and... Read more »
Osterix and RUNX2 are Transcriptional Regulators of Sclerostin in Human Bone Published: May, 2016 Abstract:
Sclerostin, encoded by the SOST gene, works as an inhibitor of the Wnt pathway and therefore is an important regulator of bone homeostasis. Due to its potent action as an inhibitor of bone formation, blocking sclerostin activity is the purpose of ... Read more »
Analysis of EZH2: micro-RNA network in low and high grade astrocytic tumors Published: January, 2016 Abstract:
Enhancer of Zeste homologue2 (EZH2) is an epigenetic regulator that functions as oncogene in astrocytic tumors, however, EZH2 regulation remains little studied. In this study, we measured EZH2 levels in low (Gr-II,DA) and high grade (Gr-IV,GBM) as... Read more »
Citrate modulates lipopolysaccharide-induced monocyte inflammatory responses. Published: June, 2015 Abstract:
Citrate, a central component of cellular metabolism, is a widely used anti-coagulant due to its ability to chelate calcium. Adenosine triphosphate (ATP)-citrate lyase, which metabolizes citrate, has been shown to be essential for inflammation, but... Read more »
NFIL3 orchestrates the emergence of common helper innate lymphoid cell precursors. Published: March, 2015 Abstract:
Innate lymphoid cells (ILCs) are a family of effectors that originate from a common innate lymphoid cell progenitor. However, the transcriptional program that sets the identity of the ILC lineage remains elusive. Here, we show that NFIL3 is a crit... Read more »
Genome-wide identification of signaling center enhancers in the developing limb. Published: November, 2014 Abstract:
The limb is widely used as a model developmental system and changes to gene expression patterns in its signaling centers, notably the zone of polarizing activity (ZPA) and the apical ectodermal ridge (AER), are known to cause limb malformations an... Read more »
DNMT3L promotes quiescence in postnatal spermatogonial progenitor cells. Published: June, 2014 Abstract:
The ability of adult stem cells to reside in a quiescent state is crucial for preventing premature exhaustion of the stem cell pool. However, the intrinsic epigenetic factors that regulate spermatogonial stem cell quiescence are largely unknown. H... Read more »
FRA2 is a STAT5 target gene regulated by IL-2 in human CD4 T cells. Published: February, 2014 Abstract:
Signal transducers and activators of transcription 5(STAT5) are cytokine induced signaling proteins, which regulate key immunological processes, such as tolerance induction, maintenance of homeostasis, and CD4 T-effector cell differentiation. In t... Read more »
Geriatric muscle stem cells switch reversible quiescence into senescence. Published: February, 2014 Abstract:
Regeneration of skeletal muscle depends on a population of adult stem cells (satellite cells) that remain quiescent throughout life. Satellite cell regenerative functions decline with ageing. Here we report that geriatric satellite cells are incap... Read more »
Arabidopsis MSI1 connects LHP1 to PRC2 complexes Published: June, 2013 Abstract:
Polycomb group (PcG) proteins form essential epigenetic memory systems for controlling gene expression during development in plants and animals. However, the mechanism of plant PcG protein functions remains poorly understood. Here, we probed the c... Read more »
ASCL1 reprograms mouse Muller glia into neurogenic retinal progenitors. Published: June, 2013 Abstract:
Non-mammalian vertebrates have a robust ability to regenerate injured retinal neurons from Müller glia (MG) that activate the gene encoding the proneural factor Achaete-scute homolog 1 (Ascl1; also known as Mash1 in mammals) and de-differentiate i... Read more »
MTA3 regulates CGB5 and Snail genes in trophoblast. Published: April, 2013 Abstract:
Secreted by the placental trophoblast, human chorionic gonadotropin (hCG) is an important hormone during pregnancy and is required for the maintenance of pregnancy. Previous studies have shown that dys-regulation of hCG expression is associated wi... Read more »
Histone-methyltransferase MLL2 (KMT2B) is required for memory formation in mice. Published: February, 2013 Abstract:
The consolidation of long-term memories requires differential gene expression. Recent research has suggested that dynamic changes in chromatin structure play a role in regulating the gene expression program linked to memory formation. The contribu... Read more »
Intronic RNAs mediate EZH2 regulation of epigenetic targets. Published: June, 2012 Abstract:
Epigenetic deregulation at a number of genomic loci is one of the hallmarks of cancer. A role for some RNA molecules in guiding repressive polycomb complex PRC2 to specific chromatin regions has been proposed. Here we use an in vivo cross-linking ... Read more »
The histone demethylase Kdm3a is essential to progression through differentiation. Published: May, 2012 Abstract:
Histone demethylation has important roles in regulating gene expression and forms part of the epigenetic memory system that regulates cell fate and identity by still poorly understood mechanisms. Here, we examined the role of histone demethylase K... Read more »
A methyl-deficient diet modifies early B cell development. Published: April, 2012 Abstract:
A functional methyl group donor is essential for the epigenetic regulation of all biological events due to the importance of DNA methylation and histone methylation as an epigenetic marker. However, the epigenetic alterations in the immune system ... Read more »
Sonic hedgehog regulates Bmi1 in human medulloblastoma brain tumor-initiating cells. Published: January, 2012 Abstract:
Bmi1 is a key stem cell regulatory gene implicated in the pathogenesis of many aggressive cancers, including medulloblastoma. Overexpression of Bmi1 promotes cell proliferation and is required for hedgehog (Hh) pathway-driven tumorigenesis. This s... Read more »
Epigenetic control of somatostatin and cortistatin expression by β amyloid peptide. Published: January, 2012 Abstract:
β Amyloid, present in senile plaques, has been related largely to neuronal loss in the brain of patients with Alzheimer's disease. However, how neurons respond to β amyloid insults is still poorly understood. Here we show that β amyloid increases ... Read more »
Histone tail acetylation in brain occurs in an unpredictable fashion after death. Published: September, 2011 Abstract:
Histone acetylation plays a role in the regulation of gene transcription. Yet it is not known whether post-mortem brain tissue is suitable for the analysis of histone acetylation. To examine this question, nucleosomes were isolated from frontal co... Read more »
Epigenetic profile of the euchromatic region of human Y chromosome. Published: May, 2011 Abstract:
The genome of a multi-cellular organism acquires various functional capabilities in different cell types by means of distinct chromatin modifications and packaging states. Acquired during early development, the cell type-specific epigenotype is ma... Read more »
Epigenetic regulation of miR-212 expression in lung cancer. Published: January, 2011 Abstract:
Many studies have shown that microRNA expression in cancer may be regulated by epigenetic events. Recently, we found that in lung cancer miR-212 was strongly down-regulated. However, mechanisms involved in the regulation of miR-212 expression are ... Read more »
Epigenetic activation of SOX11 in lymphoid neoplasms by histone modifications. Published: January, 2011 Abstract:
Recent studies have shown aberrant expression of SOX11 in various types of aggressive B-cell neoplasms. To elucidate the molecular mechanisms leading to such deregulation, we performed a comprehensive SOX11 gene expression and epigenetic study in ... Read more »
DNA methylation and Yin Yang-1 repress adenosine A2A receptor levels in human brain. Published: October, 2010 Abstract:
Adenosine A(2A) receptors (A(2A) Rs) are G-protein coupled receptors that stimulate adenylyl cyclase activity. The most A(2A) Rs-enriched brain region is the striatum, in which A(2A) Rs are largely restricted to GABAergic neurons of the indirect p... Read more »
Chromatin-Remodeling Components of the BAF Complex Facilitate Reprogramming. Published: June, 2010 Abstract:
Reprogramming of somatic cells achieved by combination of the four transcription factors Oct4, Sox2, Klf4, and c-Myc has very low efficiency. To increase the reprogramming efficiency and better understand the process, we sought to identify factors... Read more »
DNA methylation regulates adenosine A(2A) receptor cell surface expression levels. Published: March, 2010 Abstract:
Adenosine A(2A) receptors (A(2A)Rs) appear to play important roles in inflammation and in certain diseases of the nervous system. Pharmacological modulation of A(2A)Rs is particularly useful in Parkinson's disease and has been tested in schizophre... Read more »
AGRONOMICS1: a new resource for Arabidopsis transcriptome profiling. Published: February, 2010 Abstract:
Transcriptome profiling has become a routine tool in biology. For Arabidopsis (Arabidopsis thaliana), the Affymetrix ATH1 expression array is most commonly used, but it lacks about one-third of all annotated genes present in the reference strain. ... Read more »
Sox3: a transcription factor for Cyp19 expression in the frog Rana rugosa. Published: September, 2009 Abstract:
Cyp19 is expressed at a high level in the gonad of the female tadpole of the frog Rana rugosa during sex determination. To identify sequence elements important for expression of Cyp19, we isolated a genomic clone (approximately 40 kbp) carrying R.... Read more »
