Tags | Diagenode

Molecular Characterization and Event-Specific Real-Time PCR Detection of Two Dissimilar Groups of Genetically Modified Petunia (Petunia x hybrida) Sold on the Market
Published: July, 2020

Abstract: Petunia plants with unusual orange flowers were noticed on the European market and confirmed to be genetically modified (GM) by the Finnish authorities in spring 2017. Later in 2017, inspections and controls performed by several official laborator...
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Metabolic and Innate Immune Cues Merge into a Specific Inflammatory Response via the UPR.
Published: May, 2019

Abstract: Innate immune responses are intricately linked with intracellular metabolism of myeloid cells. Toll-like receptor (TLR) stimulation shifts intracellular metabolism toward glycolysis, while anti-inflammatory signals depend on enhanced mitochondrial...
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Aster Proteins Facilitate Nonvesicular Plasma Membrane to ER Cholesterol Transport in Mammalian Cells.
Published: October, 2018

Abstract: The mechanisms underlying sterol transport in mammalian cells are poorly understood. In particular, how cholesterol internalized from HDL is made available to the cell for storage or modification is unknown. Here, we describe three ER-resident pro...
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Development of PCR screening assays focused on genecoding sequences for GMO detection
Published: September, 2018

Abstract: For the detection of genetically modifed organisms (GMO), screening is the frst step used to determine if a GMO or its derived products are present in food or feed. If the result is positive, additional tests must be done to identify and, where ne...
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Development of Real-time PCR Assays for the Detection of the pin II Terminator (tpinII) Used in GM Constructs and Its Donor Organism, Potato (Solanum tuberosum)
Published: February, 2018

Abstract: G-rich DNA sequences can form four-stranded G-quadruplex (G4) secondary structures and are linked to fundamental biological processes such as transcription, replication and telomere maintenance. G4s are also implicated in promoting genome instabil...
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Detection of Hermetia illucens by real-time PCR
Published: January, 2018

Abstract: Insects are rich in proteins and could be an alternative source of macronutrients to feed animals and humans. Over the past few years, numerous companies have started producing insects for feed purposes. In Europe, the processed animal proteins ob...
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Impact of Lactobacillus plantarum ST-III on the composition of infant gut microbiota and its potential synergism with breast milk and infant formula as revealed by an in vitro study
Published: January, 2018

Abstract: Several studies have demonstrated the effect of probiotics on prevention of diseases in infants. However, the mechanisms remain not fully addressed. In the present study a modified standard ileal efflux medium (SIEM) was exploited in an in vitro a...
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Fast and discriminative CoSYPS detection system of viable Salmonella spp. and Listeria spp. in carcass swab samples
Published: January, 2015

Abstract: In this study, the complete CoSYPS Path Food workflow including all steps, namely swab sample enrichment, SYBR®Green qPCR detection of Salmonella spp. and Listeria spp., isolation and confirmation of the detected strain, was validated on beef carc...
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The LXR-Idol Axis Differentially Regulates Plasma LDL Levels in Primates and Mice.
Published: November, 2014

Abstract: The LXR-regulated E3 ubiquitin ligase IDOL controls LDLR receptor stability independent of SREBP and PCSK9, but its relevance to plasma lipid levels is unknown. Here we demonstrate that the effects of the LXR-IDOL axis are both tissue and species ...
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Comparative Study of Seven Commercial Kits for Human DNA Extraction from Urine Samples Suitable for DNA Biomarker-Based Public Health Studies
Published: October, 2014

Abstract: Human genomic DNA extracted from urine could be an interesting tool for large-scale public health studies involving characterization of genetic variations or DNA biomarkers as a result of the simple and noninvasive collection method. These studies...
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Validation of a sensitive DNA walking strategy to characterise unauthorised GMOs using model food matrices mimicking common rice products
Published: October, 2014

Abstract: To identify unauthorised GMOs in food and feed matrices, an integrated approach has recently been developed, targetting pCAMBIA family vectors, abundant in transgenic plants. Their presence is first assessed by qPCR screening and is subsequently c...
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Transducin-like enhancer of split 3 (TLE3) in adipose tissue is increased in situations characterized by decreased PPARγ gene expression.
Published: September, 2014

Abstract: Transgenic overexpression of adipose tissue (AT) transducin-like enhancer of split 3 (TLE3) mimicked peroxisome proliferator-activated receptor gamma (PPARγ) agonists, improving insulin resistance in mice. This study aimed to investigate TLE3 gene...
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The active enhancer network operated by liganded RXR supports angiogenic activity in macrophages.
Published: July, 2014

Abstract: RXR signaling is predicted to have a major impact in macrophages, but neither the biological consequence nor the genomic basis of its ligand activation is known. Comprehensive genome-wide studies were carried out to map liganded RXR-mediated trans...
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The Impact of Breastfeeding on Nasopharyngeal Microbial Communities in Infants.
Published: June, 2014

Abstract: Rationale. Breastfeeding elicits significant protection against respiratory tract infections in infancy. Modulation of respiratory microbiota might be part of the natural mechanisms of protection against respiratory diseases induced by breastfeedi...
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Evaluation of viability-qPCR detection system on viable and dead Salmonella serovar Enteritidis.
Published: June, 2014

Abstract: The propidium monoazide (PMA) coupled with PCR (viability PCR) is used in foodborne pathogen detection in order to detect only viable bacteria. Originally presented to fully remove the signal of dead bacteria, the limits of the viability PCR rapid...
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Detecting authorized and unauthorized genetically modified organisms containing vip3A by real-time PCR and next-generation sequencing.
Published: April, 2014

Abstract: The growing number of biotech crops with novel genetic elements increasingly complicates the detection of genetically modified organisms (GMOs) in food and feed samples using conventional screening methods. Unauthorized GMOs (UGMOs) in food and fe...
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In ovo inoculation of chicken embryos with probiotic bacteria and its effect on posthatch Salmonella susceptibility.
Published: April, 2014

Abstract: The feasibility of establishing probiotic bacteria in the intestine of broiler chickens by in ovo inoculation was investigated, followed by verifying possible subsequent protection against Salmonella Enteriditis infection. In a first study, 7 comm...
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The NS5A-binding heat shock proteins HSC70 and HSP70 play distinct roles in the hepatitis C viral life cycle.
Published: April, 2014

Abstract: We previously identified HSP70 and HSC70 in complex with NS5A in a proteomic screen. Here, coimmunoprecipitation studies confirmed NS5A/HSC70 complex formation during infection, and immunofluorescence studies showed NS5A and HSC70 to colocalize. U...
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Inter-laboratory Testing of GMO Detection by Combinatory SYBR®Green PCR Screening (CoSYPS)
Published: March, 2014

Abstract: Combinatory SYBR®Green real-time PCR Screening (CoSYPS) is an efficient, sensitive approach for detecting complex targets such as genetically modified organisms (GMOs) in food and feed products. GMO analysis for legal purposes has become increasin...
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Stem Cells Protect the Bronchial Stump in Rat, Increasing Sox6, Col2a1, and Agc1 Expression.
Published: March, 2014

Abstract: BACKGROUND: Post-pneumonectomy bronchopleural fistulas (BPFs) are associated with high morbidity and mortality. Currently, since the management of BPFs is difficult to assess, the best therapeutic approach is prevention. Our objective was to evalu...
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An innovative and integrated approach based on DNA walking to identify unauthorised GMOs.
Published: March, 2014

Abstract: In the coming years, the frequency of unauthorised genetically modified organisms (GMOs) being present in the European food and feed chain will increase significantly. Therefore, we have developed a strategy to identify unauthorised GMOs containin...
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A high-throughput method for GMO multi-detection using a microfluidic dynamic array.
Published: February, 2014

Abstract: The ever-increasing production of genetically modified crops generates a demand for high-throughput DNA-based methods for the enforcement of genetically modified organisms (GMO) labelling requirements. The application of standard real-time PCR wil...
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Simvastatin impairs growth hormone-activated signal transducer and activator of transcription (STAT) signaling pathway in UMR-106 osteosarcoma cells.
Published: January, 2014

Abstract: Recent studies have demonstrated that statins reduce cell viability and induce apoptosis in various types of cancer cells. The molecular mechanisms underlying these effects are poorly understood. The JAK/STAT pathway plays an important role in the...
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Reprogramming of lysosomal gene expression by interleukin-4 and Stat6
Published: December, 2013

Abstract: Background Lysosomes play important roles in multiple aspects of physiology, but the problem of how the transcription of lysosomal genes is coordinated remains incompletely understood. The goal of this study was to illuminate the physiological c...
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LXRs regulate ER stress and inflammation through dynamic modulation of membrane phospholipid composition.
Published: November, 2013

Abstract: The fatty acyl composition of phospholipids determines the biophysical character of membranes and impacts the function of membrane proteins. Here, we define a nuclear receptor pathway for the dynamic modulation of membrane composition in response ...
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Practical Experiences with an Extended Screening Strategy for Genetically Modified Organisms (GMOs) in Real-Life Samples.
Published: September, 2013

Abstract: Nowadays most animal feed products imported into Europe have a GMO (genetically modified organism) label. This means that they contain European Union (EU)-authorized GMOs. For enforcement of these labeling requirements, it is necessary, with the r...
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Quantification of biomass and cell motion in human pluripotent stem cell colonies.
Published: August, 2013

Abstract: Somatic cell reprogramming to pluripotency requires an immediate increase in cell proliferation and reduction in cell size. It is unknown whether proliferation and biomass controls are similarly coordinated with early events during the differentia...
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Reciprocal regulation of hepatic and adipose lipogenesis by liver x receptors in obesity and insulin resistance.
Published: July, 2013

Abstract: Liver X receptors (LXRs) regulate lipogenesis and inflammation, but their contribution to the metabolic syndrome is unclear. We show that LXRs modulate key aspects of the metabolic syndrome in mice. LXRαβ-deficient-ob/ob (LOKO) mice remain obese b...
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Coordinate changes in histone modifications, mRNA levels, and metabolite profiles in clonal INS-1 832/13 β-cells accompany functional adaptations to lipotoxicity.
Published: April, 2013

Abstract: Lipotoxicity is a presumed pathogenetic process whereby elevated circulating and stored lipids in type 2 diabetes cause pancreatic β-cell failure. To resolve the underlying molecular mechanisms, we exposed clonal INS-1 832/13 β-cells to palmitate ...
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Adipose subtype-selective recruitment of TLE3 or Prdm16 by PPARγ specifies lipid storage versus thermogenic gene programs.
Published: March, 2013

Abstract: Transcriptional effectors of white adipocyte-selective gene expression have not been described. Here we show that TLE3 is a white-selective cofactor that acts reciprocally with the brown-selective cofactor Prdm16 to specify lipid storage and therm...
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Bone marrow NR4A expression is not a dominant factor in the development of atherosclerosis or macrophage polarization in mice.
Published: January, 2013

Abstract: The formation of the atherosclerotic lesion is a complex process influenced by an array of inflammatory and lipid metabolic pathways. We previously demonstrated that NR4A nuclear receptors are highly induced in macrophages in response to inflammat...
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Autologous platelet-poor plasma decreases the bronchial stump necrosis in rat
Published: January, 2013

Abstract: Background: Necrosis of the bronchial stump is a very important trigger for bronchopleural fistula. The administration of local autologous platelet-poor plasma (PPP) could protect the bronchial stump. Materials and methods: Left pneumonectomy was ...
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Simulation of between Repeat Variability in Real Time PCR Reactions.
Published: November, 2012

Abstract: While many decisions rely on real time quantitative PCR (qPCR) analysis few attempts have hitherto been made to quantify bounds of precision accounting for the various sources of variation involved in the measurement process. Besides influences of...
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Vestigial-like 3 (Vgll3) is an Inhibitor of Adipocyte Differentiation.
Published: November, 2012

Abstract: Abstract Abstract Adipose differentiation is a complex process controlled by a network of transcription factors and co-regulators. We compared the global gene expression patterns of adipogenic and non-adipogenic clones of 3T3-F442A pre-adipocytes...
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Development and validation of qualitative SYBR®Green Real-Time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes.
Published: October, 2012

Abstract: A combination of four qualitative SYBR®Green qPCR screening assays targeting two levels of discrimination: Listeria genus (except Listeria grayi) and Listeria monocytogenes, is presented. These assays have been developed to be run simultaneously u...
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Divergent antiviral effects of bioflavonoids on the hepatitis C virus life cycle.
Published: September, 2012

Abstract: We have previously demonstrated that quercetin, a bioflavonoid, blocks hepatitis C virus (HCV) proliferation by inhibiting NS5A-driven internal ribosomal entry site (IRES)-mediated translation of the viral genome. Here, we investigate the mechanis...
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HOXB7 promotes invasion and predicts survival in pancreatic adenocarcinoma.
Published: August, 2012

Abstract: BACKGROUND: The homeobox gene HOXB7 is overexpressed across a range of cancers and promotes tumorigenesis through varying effects on proliferation, survival, invasion, and angiogenesis. Although published microarray data suggest HOXB7 is overexpr...
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Influence of Neonatal Hypothyroidism on Hepatic Gene Expression and Lipid Metabolism in Adulthood
Published: May, 2012

Abstract: Not Available
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A quantitative approach towards a better understanding of the dynamics of Salmonella spp. in a pork slaughter-line.
Published: February, 2012

Abstract: Pork contributes significantly to the public health disease burden caused by Salmonella infections. During the slaughter process pig carcasses can become contaminated with Salmonella. Contamination at the slaughter-line is initiated by pigs carryi...
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IL-31 regulates differentiation and filaggrin expression in human organotypic skin models.
Published: February, 2012

Abstract: BACKGROUND: Atopic dermatitis (AD) is an inflammatory skin disease affecting 10% to 20% of children and 1% to 3% of adults in industrialized countries. Enhanced expression of IL-31 is detected in skin samples of patients with AD, but its physiolog...
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Searching for novel molecular targets of chronic rejection in an orthotopic experimental lung transplantation model.
Published: February, 2012

Abstract: BACKGROUND: Chronic rejection (CR) is the main reason for the limited survival rates among lung transplant (LT) recipients. There remains no effective treatment for CR. The aim of this study was to identify new molecular mechanisms involved in CR ...
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Rapid, massively parallel single-cell drug response measurements via live cell interferometry.
Published: September, 2011

Abstract: A central question in cancer therapy is how individual cells within a population of tumor cells respond to drugs designed to arrest their growth. However, the absolute growth of cells, their change in physical mass, whether cancerous or physiologi...
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TLE3 is a dual-function transcriptional coregulator of adipogenesis.
Published: April, 2011

Abstract: PPARγ and Wnt signaling are central positive and negative regulators of adipogenesis, respectively. Here we identify the groucho family member TLE3 as a transcriptional integrator of the PPARγ and Wnt pathways. TLE3 is a direct target of PPARγ tha...
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The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats
Published: July, 2010

Abstract: Maintenance of specific heterochromatic domains is crucial for genome stability. In eukaryotic cells, a fraction of the tandem-repeated ribosomal RNA (rRNA) genes is organized in the heterochromatic structures. The principal determinant of rDNA si...
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Use of pJANUS-02-001 as a calibrator plasmid for Roundup Ready soybean event GTS-40-3-2 detection: an interlaboratory trial assessment.
Published: March, 2010

Abstract: Owing to the labelling requirements of food and feed products containing materials derived from genetically modified organisms, quantitative detection methods have to be developed for this purpose, including the necessary certified reference mater...
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Increased efficacy for in-house validation of real-time PCR GMO detection methods.
Published: March, 2010

Abstract: To improve the efficacy of the in-house validation of GMO detection methods (DNA isolation and real-time PCR, polymerase chain reaction), a study was performed to gain insight in the contribution of the different steps of the GMO detection method ...
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Antiangiogenic liposomal gene therapy with 16K human prolactin efficiently reduces tumor growth.
Published: November, 2009

Abstract: Human 16K PRL (16K hPRL) is a potent inhibitor of angiogenesis both in vitro and in vivo. It has been shown to prevent tumor growth in three xenograft mouse models. Here we have used a gene transfer method based on cationic liposomes to produce 16...
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Epigenetic engineering of ribosomal RNA genes enhances protein production.
Published: January, 2009

Abstract: Selection of mammalian high-producer cell lines remains a major challenge for the biopharmaceutical manufacturing industry. Ribosomal RNA (rRNA) genes encode the major component of the ribosome but many rRNA gene copies are not transcribed due to ...
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LXR signaling couples sterol metabolism to proliferation in the acquired immune response.
Published: July, 2008

Abstract: Cholesterol is essential for membrane synthesis; however, the mechanisms that link cellular lipid metabolism to proliferation are incompletely understood. We demonstrate here that cellular cholesterol levels in dividing T cells are maintained in p...
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Accuracy and potential usefulness of triplex real-time PCR for improving antibiotic treatment of patients with blood cultures showing clustered gram-positive cocci on direct smears.
Published: June, 2008

Abstract: Bacterial identification and antibiotic susceptibility testing currently require 48 h when a first blood culture (BC) is positive for clustered gram-positive cocci on direct smear examination (DSE). Meanwhile, antibiotic treatment is often inadequ...
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