Genipin as a novel chemical activator of EBV lytic cycle. Published: February, 2015 Abstract:
Epstein-Barr virus (EBV) is a ubiquitous gammaherpesvirus that causes acute infection and establishes life-long latency. EBV causes several human cancers, including Burkitt's lymphoma, nasopharyngeal and gastric carcinoma. Antiviral agents can be ... Read more »
O-GlcNAcylation Prevents Aggregation of the Polycomb Group Repressor Polyhomeotic. Published: November, 2014 Abstract:
The glycosyltransferase Ogt adds O-linked N-Acetylglucosamine (O-GlcNAc) moieties to nuclear and cytosolic proteins. Drosophila embryos lacking Ogt protein arrest development with a remarkably specific Polycomb phenotype, arising from the failure ... Read more »
In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Published: October, 2014 Abstract:
Probing gene function in the mammalian brain can be greatly assisted with methods to manipulate the genome of neurons in vivo. The clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease (Cas)9 from Streptococc... Read more »
RPLP1, a Crucial Ribosomal Protein for Embryonic Development of the Nervous System. Published: June, 2014 Abstract:
Ribosomal proteins are pivotal to development and tissue homeostasis. RP Large P1 (Rplp1) overexpression is associated with tumorigenesis. However, the physiological function of Rplp1 in mammalian development remains unknown. In this study, we dis... Read more »
ZNF143 is regulated through alternative 3'UTR isoforms. Published: June, 2014 Abstract:
ZNF143 is a ubiquitously expressed transcription factor conserved in all vertebrates, regulating genes involved in primary metabolism and cell growth. It is therefore crucial to tightly maintain the adequate level of this factor in the cell. Altho... Read more »
Unraveling vitamin B12-responsive gene regulation in algae. Published: May, 2014 Abstract:
Photosynthetic microalgae play a vital role in primary productivity and biogeochemical cycling in both marine and freshwater systems across the globe. However, the growth of these cosmopolitan organisms depends on the bioavailability of nutrients ... Read more »
Prmt7 is dispensable in tissue culture models for adipogenic differentiation Published: December, 2013 Abstract:
Protein arginine methylation is a common posttranslational modification that has been implicated in numerous biological processes including gene expression. The mammalian genome encodes nine protein arginine methyltransferases (Prmts) that catalyz... Read more »
Adenovirus precursor pVII protein stability is regulated by its propeptide sequence. Published: November, 2013 Abstract:
Adenovirus encodes for the pVII protein, which interacts and modulates virus DNA structure in the infected cells. The pVII protein is synthesized as the precursor protein and undergoes proteolytic processing by viral proteinase Avp, leading to rel... Read more »
A new strategy for gene targeting and functional proteomics using the DT40 cell line. Published: September, 2013 Abstract:
DT40 cells derived from chicken B lymphocytes exhibit exceptionally high homologous recombination rates. Therefore, they can be used as a convenient tool and model for gene targeting experiments. However, lack of efficient cloning strategies, prot... Read more »
The 26S proteasome drives trinucleotide repeat expansions. Published: July, 2013 Abstract:
Trinucleotide repeat (TNR) expansion is the causative mutation for at least 17 inherited neurological diseases. An important question in the field is which proteins drive the expansion process. This study reports that the multi-functional protein ... Read more »
Epigenetic regulation of microRNA expression in renal cell carcinoma. Published: June, 2013 Abstract:
The underlying mechanisms of microRNA deregulation in cancer cells include epigenetic modifications, which play a crucial role in carcinogenesis. We demonstrate that numerous microRNAs are induced in renal cell carcinoma cell lines after treatment... Read more »
CPEB1 coordinates alternative 3'-UTR formation with translational regulation. Published: March, 2013 Abstract:
More than half of mammalian genes generate multiple messenger RNA isoforms that differ in their 3' untranslated regions (3' UTRs) and therefore in regulatory sequences, often associated with cell proliferation and cancer; however, the mechanisms c... Read more »
Dual Role of Dextran Sulfate 5000 Da as Anti-Apoptotic and Pro-Autophagy Agent. Published: November, 2012 Abstract:
Dextran sulfate 5,000 Da (DS), a sulfated polysaccharide, has been used in recombinant mammalian cell cultures to prevent cell aggregation, thereby increasing cell viability. Previous studies using Chinese hamster ovary (CHO) suspension cultures h... Read more »
BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs. Published: November, 2012 Abstract:
The differentiation of several T- and B-cell effector programs in the immune system is directed by signature transcription factors that induce rapid epigenetic remodelling. Here we report that promyelocytic leukaemia zinc finger (PLZF), the BTB-zi... Read more »
Systematic identification of functional residues in mammalian histone H2AX. Published: October, 2012 Abstract:
The histone variant H2AX is a principal component of chromatin involved in the detection, signaling and repair of DNA double-strand breaks (DSBs). H2AX is thought to operate primarily through its C-terminal S139 phosphorylation, which mediates the... Read more »
The linker histone plays a dual role during gametogenesis in S. cerevisiae. Published: May, 2012 Abstract:
The differentiation of gametes involves dramatic changes to chromatin, affecting transcription, meiosis, and cell morphology. Sporulation in S. cerevisiae shares many chromatin features with spermatogenesis, including a ten-fold compaction of the ... Read more »
Recombineering BAC transgenes for protein tagging. Published: February, 2011 Abstract:
Protein tagging offers many advantages for proteomic and regulomic research, particularly due to the use of generic and highly sensitive methods that can be applied with reasonable throughput. Ideally, protein tagging is equivalent to having a hig... Read more »
Transcription factor IIS impacts UV-inhibited transcription. Published: November, 2010 Abstract:
Inhibition of transcription elongation can cause severe developmental and neurological abnormalities notably manifested by the rare recessive progeroid disorder Cockayne syndrome (CS). DNA alterations can cause permanent blocks to an elongating RN... Read more »
Novel snail1 target proteins in human colon cancer identified by proteomic analysis. Published: January, 2010 Abstract:
BACKGROUND: The transcription factor Snail1 induces epithelial-to-mesenchymal transition (EMT), a process responsible for the acquisition of invasiveness during tumorigenesis. Several transcriptomic studies have reported Snail1-regulated genes in ... Read more »
INTS3 controls the hSSB1-mediated DNA damage response. Published: October, 2009 Abstract:
Human SSB1 (single-stranded binding protein 1 [hSSB1]) was recently identified as a part of the ataxia telangiectasia mutated (ATM) signaling pathway. To investigate hSSB1 function, we performed tandem affinity purifications of hSSB1 mutants mimic... Read more »
Phosphorylation of histone H3 Thr-45 is linked to apoptosis. Published: June, 2009 Abstract:
Numerous post-translational modifications have been identified in histones. Most of these occur within the histone tails, but a few have been identified within the histone core sequences. Histone core post-translational modifications have the pote... Read more »
Multi-walled carbon nanotubes injure the plasma membrane of macrophages. Published: October, 2008 Abstract:
Carbon nanotubes (CNTs) are emerging nanotechnology materials which are likely to be mass-produced in the near future. However, prior to mass-production, certain health-related concerns should first be addressed. For example, when inhaled, the thi... Read more »
FoxK2 is required for cellular proliferation and survival Abstract:
FoxK2 is a forkhead transcription factor expressed ubiquitously in the developing murine central nervous system. Here we investigated the role of FoxK2 in vitro and focused on proliferation and cellular survival. Knockdown of FoxK2 results in a de... Read more »
