Antibody package for CUT&Tag (anti-rabbit)

Catalog Number

Format

Price

C01070022

24 rxns

$185.00

Other format

C01070026

8 rxns

Diagenode’s Antibody Package for CUT&Tag (anti-rabbit) has been designed to be used with the iDeal CUT&Tag for Histones Kit. This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit.

The Antibody Package for CUT&Tag (anti-rabbit) contains species-specific controls antibodies:

Anti-rabbit secondary antibody
Rabbit H3K27me3 positive control antibody
Rabbit negative IgG control
TSH2B primer pair (human)
GAPDH TSS primer pair (human)

The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.

If the primary antibody of interest used in the CUT&Tag assays has been raised in mouse, please use the Antibody Package for CUT&Tag (anti-mouse).

Check out:

Safety sheets

Antibody Package for CUT&Tag (anti-rabbit) SDS GB en	Download
Antibody Package for CUT&Tag (anti-rabbit) SDS US en	Download
Antibody Package for CUT&Tag (anti-rabbit) SDS DE de	Download
Antibody Package for CUT&Tag (anti-rabbit) SDS JP ja	Download
Antibody Package for CUT&Tag (anti-rabbit) SDS BE nl	Download
Antibody Package for CUT&Tag (anti-rabbit) SDS BE fr	Download
Antibody Package for CUT&Tag (anti-rabbit) SDS FR fr	Download
Antibody Package for CUT&Tag (anti-rabbit) SDS ES es	Download

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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&amp;Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit.</p>
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<li>GAPDH TSS primer pair (human)</li>
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<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&amp;Tag (anti-mouse)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&amp;Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit.</p>
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<li>Rabbit H3K27me3 positive control antibody</li>
<li>Rabbit negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
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<p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&amp;Tag (anti-mouse)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p>The Antibody Package for CUT&amp;Tag (anti-rabbit) contains species-specific controls antibodies:</p>
<ul>
<li>Anti-rabbit secondary antibody</li>
<li>Rabbit H3K27me3 positive control antibody</li>
<li>Rabbit negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
</ul>
<p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&amp;Tag (anti-mouse)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
</ul>',
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<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><strong>CUT&amp;Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&amp;Tag &ndash; our iDeal CUT&amp;Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein &ndash; pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&amp;Tag</a> proving their high performance in this assay.</p>
<br /> <a href="https://www.diagenode.com/files/application_notes/AN-iDealCUTandTag.pdf"><img src="https://www.diagenode.com/img/banners/cutandtag-appnote.png" /></a><br /><br /></div>
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<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a>
<div id="v5" class="content">
<p>The iDeal CUT&amp;Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode&rsquo;s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode&rsquo;s Primer Indexes for tagmented libraries.</p>
<img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div>
<h2>Products for CUT&amp;Tag assay</h2>
<h3 class="diacol">Complete solutions</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&amp;Tag kit for Histones</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">Fusion protein</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li>
</ul>
<h3 class="diacol">CUT&amp;Tag grade antibodies</h3>
<ul class="nobullet">
<li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&amp;Tag</a></li>
<li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li>
<li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&amp;Tag</a></li>
</ul>
<h3 class="diacol">Positive &amp; Negative CUT&amp;Tag control</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">DNA purification</h3>
<p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p>
<h3 class="diacol">Sequencing indexes</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li>
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<li>Rabbit negative IgG control</li>
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<li>GAPDH TSS primer pair (human)</li>
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<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&amp;Tag (anti-mouse)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&amp;Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit.</p>
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<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p><strong>Check out:</strong></p>
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<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p><strong>CUT&amp;Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&amp;Tag &ndash; our iDeal CUT&amp;Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein &ndash; pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&amp;Tag</a> proving their high performance in this assay.</p>
<br /> <a href="https://www.diagenode.com/files/application_notes/AN-iDealCUTandTag.pdf"><img src="https://www.diagenode.com/img/banners/cutandtag-appnote.png" /></a><br /><br /></div>
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<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a>
<div id="v5" class="content">
<p>The iDeal CUT&amp;Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode&rsquo;s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode&rsquo;s Primer Indexes for tagmented libraries.</p>
<img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div>
<h2>Products for CUT&amp;Tag assay</h2>
<h3 class="diacol">Complete solutions</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&amp;Tag kit for Histones</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">Fusion protein</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li>
</ul>
<h3 class="diacol">CUT&amp;Tag grade antibodies</h3>
<ul class="nobullet">
<li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&amp;Tag</a></li>
<li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li>
<li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&amp;Tag</a></li>
</ul>
<h3 class="diacol">Positive &amp; Negative CUT&amp;Tag control</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">DNA purification</h3>
<p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p>
<h3 class="diacol">Sequencing indexes</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li>
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&amp;Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit.</p>
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<li>Anti-rabbit secondary antibody</li>
<li>Rabbit H3K27me3 positive control antibody</li>
<li>Rabbit negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
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<p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&amp;Tag (anti-mouse)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&amp;Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit.</p>
<p>The Antibody Package for CUT&amp;Tag (anti-rabbit) contains species-specific controls antibodies:</p>
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<li>Anti-rabbit secondary antibody</li>
<li>Rabbit H3K27me3 positive control antibody</li>
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<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
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<p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&amp;Tag (anti-mouse)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<li>Anti-rabbit secondary antibody</li>
<li>Rabbit H3K27me3 positive control antibody</li>
<li>Rabbit negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
</ul>
<p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&amp;Tag (anti-mouse)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&amp;Tag (anti-mouse)</a>.</p>
<p><strong>Check out:</strong></p>
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<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&amp;Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit.</p>
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<li>Rabbit negative IgG control</li>
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<li>GAPDH TSS primer pair (human)</li>
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<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p><strong>CUT&amp;Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&amp;Tag &ndash; our iDeal CUT&amp;Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein &ndash; pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&amp;Tag</a> proving their high performance in this assay.</p>
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<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a>
<div id="v5" class="content">
<p>The iDeal CUT&amp;Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode&rsquo;s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode&rsquo;s Primer Indexes for tagmented libraries.</p>
<img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div>
<h2>Products for CUT&amp;Tag assay</h2>
<h3 class="diacol">Complete solutions</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&amp;Tag kit for Histones</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">Fusion protein</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li>
</ul>
<h3 class="diacol">CUT&amp;Tag grade antibodies</h3>
<ul class="nobullet">
<li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&amp;Tag</a></li>
<li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li>
<li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&amp;Tag</a></li>
</ul>
<h3 class="diacol">Positive &amp; Negative CUT&amp;Tag control</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">DNA purification</h3>
<p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p>
<h3 class="diacol">Sequencing indexes</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li>
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&amp;Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit.</p>
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<p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&amp;Tag (anti-mouse)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&amp;Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit.</p>
<p>The Antibody Package for CUT&amp;Tag (anti-rabbit) contains species-specific controls antibodies:</p>
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<li>Anti-rabbit secondary antibody</li>
<li>Rabbit H3K27me3 positive control antibody</li>
<li>Rabbit negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
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<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&amp;Tag (anti-mouse)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<li>Anti-rabbit secondary antibody</li>
<li>Rabbit H3K27me3 positive control antibody</li>
<li>Rabbit negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
</ul>
<p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&amp;Tag (anti-mouse)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
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<p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&amp;Tag (anti-mouse)</a>.</p>
<p><strong>Check out:</strong></p>
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<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p><strong>CUT&amp;Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&amp;Tag &ndash; our iDeal CUT&amp;Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein &ndash; pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&amp;Tag</a> proving their high performance in this assay.</p>
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<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a>
<div id="v5" class="content">
<p>The iDeal CUT&amp;Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode&rsquo;s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode&rsquo;s Primer Indexes for tagmented libraries.</p>
<img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div>
<h2>Products for CUT&amp;Tag assay</h2>
<h3 class="diacol">Complete solutions</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&amp;Tag kit for Histones</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">Fusion protein</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li>
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<h3 class="diacol">CUT&amp;Tag grade antibodies</h3>
<ul class="nobullet">
<li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&amp;Tag</a></li>
<li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li>
<li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&amp;Tag</a></li>
</ul>
<h3 class="diacol">Positive &amp; Negative CUT&amp;Tag control</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">DNA purification</h3>
<p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p>
<h3 class="diacol">Sequencing indexes</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li>
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<p><strong>Check out:</strong></p>
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<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p><strong>Check out:</strong></p>
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<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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$rrbs_service = array(
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)
$chipseq_service = array(
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	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
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	'antibody_id' => null,
	'name' => 'Antibody package for CUT&Tag (anti-rabbit)',
	'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-rabbit) has been designed to be used with the <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24">iDeal CUT&amp;Tag for Histones Kit</a>. This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit.</p>
<p>The Antibody Package for CUT&amp;Tag (anti-rabbit) contains species-specific controls antibodies:</p>
<ul>
<li>Anti-rabbit secondary antibody</li>
<li>Rabbit H3K27me3 positive control antibody</li>
<li>Rabbit negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
</ul>
<p>The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24">Antibody Package for CUT&amp;Tag (anti-mouse)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
</ul>',
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	'price_EUR' => '80',
	'price_USD' => '90',
	'price_GBP' => '70',
	'price_JPY' => '/',
	'price_CNY' => '/',
	'price_AUD' => '/',
	'country' => 'ALL',
	'except_countries' => 'None',
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	'modified' => '2024-01-11 11:15:13',
	'created' => '2023-12-20 14:22:03',
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		'group_id' => '366'
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	'name' => 'Antibody package for CUT&Tag (anti-rabbit)',
	'description' => '',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/cutandtag/Datasheet_Antibody-Package- for-CUTTag_anti-rabbit.pdf',
	'slug' => 'antibody-package-for-cutandtag-anti-rabbit',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2022-10-07 11:10:51',
	'created' => '2022-10-07 11:10:51',
	'ProductsDocument' => array(
		'id' => '3253',
		'product_id' => '3222',
		'document_id' => '1163'
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$sds = array(
	'id' => '2332',
	'name' => 'Antibody Package for CUT&Tag (anti-rabbit) SDS ES es',
	'language' => 'es',
	'url' => 'files/SDS/CUT_Tag/SDS-C01070022-Antibody_Package_for_CUT_Tag_anti-rabbit_-ES-es-1_0.pdf',
	'countries' => 'ES',
	'modified' => '2022-08-29 14:35:38',
	'created' => '2022-08-29 14:35:38',
	'ProductsSafetySheet' => array(
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		'product_id' => '3222',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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