The chromatin shearing is very important step for all kind of ChIP analysis followed by next-generation sequencing. The efficiency, reproducibility and straightforwardness are the most important characteristics for shearing. The using of S. cerevisiae as a model organism is even more complicated because they have a cell wall which require special lysis and can effect shearing. In this protocol we compared the yeast chromatin shearing by Covaris™ S2 and Bioruptor® Pico, and the chromatin crosslinking by 16% methanol-free and 37% methanol-containing formaldehyde.
