24 Dual indexes for MicroPlex Kit v3 /48 rxns

1 tube, 2 hours, 3 steps protocol
Input: 50 pg – 50 ng
Reduce potential bias - few PCR amplification cycles needed
High sensitivity ChIP-seq - low PCR duplication rate
Great multiplexing flexibility
Validated with the IP-Star Compact Automated Platform

How it works

Microplex workflow - protocol with dual indexes
An input of 50 pg to 50 ng of fragmented dsDNA is converted into sequencing-ready libraries for Illumina® NGS platforms using a fast and simple 3-step protocol

Read more about MicroPlex workflow

Step 1. Template Preparation provides efficient repair of the fragmented double-stranded DNA input.

In this step, the DNA is repaired and yields molecules with blunt ends.

Step 2. Library Synthesis. enables ligation of MicroPlex patented stem- loop adapters.

In the next step, stem-loop adaptors with blocked 5’ ends are ligated with high efficiency to the 5’ end of the genomic DNA, leaving a nick at the 3’ end. The adaptors cannot ligate to each other and do not have single- strand tails, both of which contribute to non-specific background found with many other NGS preparations.

Step 3. Library Amplification enables extension of the template, cleavage of the stem-loop adaptors, and amplification of the library. Illumina- compatible indexes are also introduced using a high-fidelity, highly- processive, low-bias DNA polymerase.

In the final step, the 3’ ends of the genomic DNA are extended to complete library synthesis and Illumina-compatible indexes are added through a high-fidelity amplification. Any remaining free adaptors are destroyed. Hands-on time and the risk of contamination are minimized by using a single tube and eliminating intermediate purifications.

Obtained libraries are purified, quantified and sized. The libraries pooling can be performed as well before sequencing.

Primer indexes for MicroPlex kit v3 MANUAL
High Performance Library Preparation for Illumina® NGS Platforms

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24 Dual indexes for MicroPlex Kit v3 48 rxns SDS GB en	Download
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MicroPlex Library Preparation Kit v3 SDS JP ja	Download
24 Dual indexes for MicroPlex Kit v3 48 rxns SDS ES es	Download
24 Dual indexes for MicroPlex Kit v3 48 rxns SDS DE de	Download
24 Dual indexes for MicroPlex Kit v3 48 rxns SDS JP ja	Download

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Supraphysiological Androgens Promote the Tumor Suppressive Activity of the Androgen Receptor Through cMYC Repression and Recruitment of the DREAM Complex
Nyquist M. et al.
The androgen receptor (AR) pathway regulates key cell survival programs in prostate epithelium. The AR represents a near-universal driver and therapeutic vulnerability in metastatic prostate cancer, and targeting AR has a remarkable therapeutic index. Though most approaches directed toward AR focus on inhibiting AR ...

Gene Regulatory Interactions at Lamina-Associated Domains
Madsen-Østerbye J. et al.
The nuclear lamina provides a repressive chromatin environment at the nuclear periphery. However, whereas most genes in lamina-associated domains (LADs) are inactive, over ten percent reside in local euchromatic contexts and are expressed. How these genes are regulated and whether they are able to interact with regu...

Mechanisms and function of de novo DNA methylation in placentaldevelopment reveals an essential role for DNMT3B.
Andrews S. et al.
DNA methylation is a repressive epigenetic modification that is essential for development, exemplified by the embryonic and perinatal lethality observed in mice lacking de novo DNA methyltransferases (DNMTs). Here we characterise the role for DNMT3A, 3B and 3L in gene regulation and development of the mouse placenta...

Heterogeneity of neurons reprogrammed from spinal cord astrocytes by theproneural factors Ascl1 and Neurogenin2
Kempf J. et al.
Summary Astrocytes are a viable source for generating new neurons via direct conversion. However, little is known about the neurogenic cascades triggered in astrocytes from different regions of the CNS. Here, we examine the transcriptome induced by the proneural factors Ascl1 and Neurog2 in spinal cord-derived astro...

Lasp1 regulates adherens junction dynamics and fibroblast transformationin destructive arthritis
Beckmann D. et al.
The LIM and SH3 domain protein 1 (Lasp1) was originally cloned from metastatic breast cancer and characterised as an adaptor molecule associated with tumourigenesis and cancer cell invasion. However, the regulation of Lasp1 and its function in the aggressive transformation of cells is unclear. Here we use integrativ...

Age-associated cryptic transcription in mammalian stem cells is linked topermissive chromatin at cryptic promoters
McCauley B. S. et al.
Suppressing spurious cryptic transcription by a repressive intragenic chromatin state featuring trimethylated lysine 36 on histone H3 (H3K36me3) and DNA methylation is critical for maintaining self-renewal capacity in mouse embryonic stem cells. In yeast and nematodes, such cryptic transcription is elevated with age...

C05010001

MicroPlex Library Preparation Kit v3 /48 rxns

24 Dual indexes for MicroPlex Kit v3 /48 rxns

How it works

How to properly cite our product/service in your work

MicroPlex Library Preparation Kit v3 /48 rxns

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