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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with AsCRISPR/Cpf1 using the Diagenode antibody against AsCRISPR/Cpf1 (Cat. No. C15310262), diluted 1:5,000 in PBS-T containing 3&percent; NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (Cat. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (Cat. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<li>Raised against the Cpf1 nuclease from <em>Acidaminococcus sp.</em></li>
<li>Ideal for Western blot</li>
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<div class="row">
<div class="small-3 medium-3 large-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig1.png" /></div>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<li>Raised against the Cpf1 nuclease from <em>Lachnospiraceae bacterium</em></li>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with AsCRISPR/Cpf1 using the Diagenode antibody against AsCRISPR/Cpf1 (Cat. No. C15310262), diluted 1:5,000 in PBS-T containing 3&percent; NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (Cat. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (Cat. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<li>Raised against the Cpf1 nuclease from <em>Acidaminococcus sp.</em></li>
<li>Ideal for Western blot</li>
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<div class="small-3 medium-3 large-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig1.png" /></div>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<li>Raised against the Cpf1 nuclease from <em>Lachnospiraceae bacterium</em></li>
<li>Validated in Western blot, <g class="gr_ gr_22 gr-alert gr_gramm gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="22" data-gr-id="22">immunoprecipitation</g> and immunofluorescence</li>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with AsCRISPR/Cpf1 using the Diagenode antibody against AsCRISPR/Cpf1 (Cat. No. C15310262), diluted 1:5,000 in PBS-T containing 3&percent; NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (Cat. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells (lane 1), HEK293 cells transfected with AsCRISPR/Cpf1 (lane 2) and HEK293 cells transfected with LbCRISPR/Cpf1 (lane 3) using the Diagenode antibody against AsCRISPR/Cpf1 (Cat. No. C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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×
