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Monoclonal antibody raised in mouse against Acidaminococcus sp. (As) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.
| Lot | 001 |
|---|---|
| Concentration | 1 µg/µl |
| Species reactivity | Acidaminococcus sp. |
| Type | Monoclonal |
| Purity | Protein G purified monoclonal antibody |
| Host | Mouse |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | TBS containing 0.01 % Na-azide |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Western blotting | 1:1,000 | Fig 1 |
Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1
Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.
| Acidaminococcus sp. CRISPR/Cpf1 monoclonal antibody DATASHEET Monoclonal antibody raised in mouse against Acidaminococcus sp. (As) Cpf1 (CRISPR from Prevotella... | Download |
| Acidaminococcus sp. CRISPR Cpf1 antibody SDS GB en | Download |
| Acidaminococcus sp. CRISPR Cpf1 antibody SDS US en | Download |
| Acidaminococcus sp. CRISPR Cpf1 antibody SDS DE de | Download |
| Acidaminococcus sp. CRISPR Cpf1 antibody SDS JP ja | Download |
| Acidaminococcus sp. CRISPR Cpf1 antibody SDS BE nl | Download |
| Acidaminococcus sp. CRISPR Cpf1 antibody SDS BE fr | Download |
| Acidaminococcus sp. CRISPR Cpf1 antibody SDS FR fr | Download |
| Acidaminococcus sp. CRISPR Cpf1 antibody SDS ES es | Download |
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CRISPR/Cpf1 Monoclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2954', 'antibody_id' => '521', 'name' => '<em>Acidaminococcus sp.</em> CRISPR/Cpf1 Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against <em>Acidaminococcus sp.</em> (As) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200234-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). 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In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. 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CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.' $meta_title = 'Acidaminococcus sp. CRISPR/Cpf1 Monoclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2954', 'antibody_id' => '521', 'name' => '<em>Acidaminococcus sp.</em> CRISPR/Cpf1 Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against <em>Acidaminococcus sp.</em> (As) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200234-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). 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In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. 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Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). 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Sample size available.', 'modified' => '2022-01-05 15:13:27', 'created' => '2018-02-22 15:55:49' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '123', 'position' => '4', 'parent_id' => '100', 'name' => 'CRISPR/Cpf1 antibodies', 'description' => '<p>We are proud to offer the first antibodies specifically recognizing the Cpf1 nuclease, described as a potential candidate for genome editing in mammalian cells.</p> <h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2> <ul> <li>Raised against the Cpf1 nuclease from <em>Acidaminococcus sp.</em></li> <li>Ideal for Western blot</li> </ul> <div class="row"> <div class="small-3 medium-3 large-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig1.png" /></div> <div class="small-9 medium-9 large-9 columns"> <p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p> </div> </div> <h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2> <ul> <li>Raised against the Cpf1 nuclease from <em>Lachnospiraceae bacterium</em></li> <li>Validated in Western blot, <g class="gr_ gr_22 gr-alert gr_gramm gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="22" data-gr-id="22">immunoprecipitation</g> and immunofluorescence</li> </ul> <div class="row"> <div class="small-5 medium-5 large-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig2.png" /></div> <div class="small-7 medium-7 large-7 columns"> <p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'Acidaminococcus sp. CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.', 'meta_title' => 'Acidaminococcus sp. CRISPR/Cpf1 Monoclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2954', 'antibody_id' => '521', 'name' => '<em>Acidaminococcus sp.</em> CRISPR/Cpf1 Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against <em>Acidaminococcus sp.</em> (As) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200234-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). 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Two of these CRISPR/Cpf1 systems, present in <em>Acidaminococcus sp.</em> and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.', 'clonality' => '', 'isotype' => 'IgG2a', 'lot' => '001', 'concentration' => '1 µg/µl', 'reactivity' => '<em>Acidaminococcus sp.</em>', 'type' => 'Monoclonal', 'purity' => 'Protein G purified monoclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 1</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'TBS containing 0.01 % Na-azide', 'precautions' => 'This product is for research use only. 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Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). 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In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. 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Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). 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Sample size available.', 'modified' => '2022-01-05 15:13:27', 'created' => '2018-02-22 15:55:49' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '123', 'position' => '4', 'parent_id' => '100', 'name' => 'CRISPR/Cpf1 antibodies', 'description' => '<p>We are proud to offer the first antibodies specifically recognizing the Cpf1 nuclease, described as a potential candidate for genome editing in mammalian cells.</p> <h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2> <ul> <li>Raised against the Cpf1 nuclease from <em>Acidaminococcus sp.</em></li> <li>Ideal for Western blot</li> </ul> <div class="row"> <div class="small-3 medium-3 large-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig1.png" /></div> <div class="small-9 medium-9 large-9 columns"> <p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p> </div> </div> <h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2> <ul> <li>Raised against the Cpf1 nuclease from <em>Lachnospiraceae bacterium</em></li> <li>Validated in Western blot, <g class="gr_ gr_22 gr-alert gr_gramm gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="22" data-gr-id="22">immunoprecipitation</g> and immunofluorescence</li> </ul> <div class="row"> <div class="small-5 medium-5 large-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig2.png" /></div> <div class="small-7 medium-7 large-7 columns"> <p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'locale' => 'eng' ) $description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'Acidaminococcus sp. CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.', 'meta_title' => 'Acidaminococcus sp. CRISPR/Cpf1 Monoclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2954', 'antibody_id' => '521', 'name' => '<em>Acidaminococcus sp.</em> CRISPR/Cpf1 Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against <em>Acidaminococcus sp.</em> (As) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200234-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200234-100', 'old_catalog_number' => '', 'sf_code' => 'C15200234-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '555', 'price_USD' => '580', 'price_GBP' => '500', 'price_JPY' => '86940', 'price_CNY' => '0', 'price_AUD' => '1450', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 6, 2018', 'slug' => 'acidaminococcus-sp-crisprcpf1-monoclonal-antibody-100-ul', 'meta_title' => 'Acidaminococcus sp. CRISPR/Cpf1 Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Acidaminococcus sp. CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. 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In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. Two of these CRISPR/Cpf1 systems, present in <em>Acidaminococcus sp.</em> and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.', 'clonality' => '', 'isotype' => 'IgG2a', 'lot' => '001', 'concentration' => '1 µg/µl', 'reactivity' => '<em>Acidaminococcus sp.</em>', 'type' => 'Monoclonal', 'purity' => 'Protein G purified monoclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 1</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'TBS containing 0.01 % Na-azide', 'precautions' => 'This product is for research use only. 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CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.' $meta_title = 'Acidaminococcus sp. CRISPR/Cpf1 Monoclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2954', 'antibody_id' => '521', 'name' => '<em>Acidaminococcus sp.</em> CRISPR/Cpf1 Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against <em>Acidaminococcus sp.</em> (As) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200234-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). 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CRISPR/Cpf1 monoclonal antibody (001 - 1 µg/µl - <em>Acidaminococcus sp.</em> - Protein G purified monoclonal antibody - Mouse)' ), 'Slave' => array( (int) 0 => array( 'id' => '244', 'name' => 'C15200234', 'product_id' => '2954', 'modified' => '2018-02-22 16:02:08', 'created' => '2018-02-22 16:02:08' ) ), 'Group' => array( 'Group' => array( 'id' => '244', 'name' => 'C15200234', 'product_id' => '2954', 'modified' => '2018-02-22 16:02:08', 'created' => '2018-02-22 16:02:08' ), 'Master' => array( 'id' => '2954', 'antibody_id' => '521', 'name' => '<em>Acidaminococcus sp.</em> CRISPR/Cpf1 Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against <em>Acidaminococcus sp.</em> (As) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200234-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '123', 'position' => '4', 'parent_id' => '100', 'name' => 'CRISPR/Cpf1 antibodies', 'description' => '<p>We are proud to offer the first antibodies specifically recognizing the Cpf1 nuclease, described as a potential candidate for genome editing in mammalian cells.</p> <h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2> <ul> <li>Raised against the Cpf1 nuclease from <em>Acidaminococcus sp.</em></li> <li>Ideal for Western blot</li> </ul> <div class="row"> <div class="small-3 medium-3 large-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig1.png" /></div> <div class="small-9 medium-9 large-9 columns"> <p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p> </div> </div> <h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2> <ul> <li>Raised against the Cpf1 nuclease from <em>Lachnospiraceae bacterium</em></li> <li>Validated in Western blot, <g class="gr_ gr_22 gr-alert gr_gramm gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="22" data-gr-id="22">immunoprecipitation</g> and immunofluorescence</li> </ul> <div class="row"> <div class="small-5 medium-5 large-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig2.png" /></div> <div class="small-7 medium-7 large-7 columns"> <p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'carrousel_order' => array( (int) 0 => 'Gene editing' ), 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'Acidaminococcus sp. CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.', 'meta_title' => 'Acidaminococcus sp. CRISPR/Cpf1 Monoclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2954', 'antibody_id' => '521', 'name' => '<em>Acidaminococcus sp.</em> CRISPR/Cpf1 Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against <em>Acidaminococcus sp.</em> (As) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200234-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). 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Two of these CRISPR/Cpf1 systems, present in <em>Acidaminococcus sp.</em> and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.', 'clonality' => '', 'isotype' => 'IgG2a', 'lot' => '001', 'concentration' => '1 µg/µl', 'reactivity' => '<em>Acidaminococcus sp.</em>', 'type' => 'Monoclonal', 'purity' => 'Protein G purified monoclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 1</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'TBS containing 0.01 % Na-azide', 'precautions' => 'This product is for research use only. 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CRISPR/Cpf1 monoclonal antibody (001 - 1 µg/µl - <em>Acidaminococcus sp.</em> - Protein G purified monoclonal antibody - Mouse)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/acidaminococcus-sp-crisprcpf1-monoclonal-antibody-100-ul' ) $carrousel_order = array( (int) 0 => 'Gene editing' ) $language = 'en' $meta_keywords = '' $meta_description = 'Acidaminococcus sp. CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.' $meta_title = 'Acidaminococcus sp. CRISPR/Cpf1 Monoclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2954', 'antibody_id' => '521', 'name' => '<em>Acidaminococcus sp.</em> CRISPR/Cpf1 Antibody', 'description' => '<p>Monoclonal antibody raised in mouse against <em>Acidaminococcus sp.</em> (As) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200234-wb.jpg" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). 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Sample size available.', 'modified' => '2022-01-05 15:13:27', 'created' => '2018-02-22 15:55:49', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '521', 'name' => 'Acidaminococcus sp. CRISPR/Cpf1 monoclonal antibody', 'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. Two of these CRISPR/Cpf1 systems, present in <em>Acidaminococcus sp.</em> and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.', 'clonality' => '', 'isotype' => 'IgG2a', 'lot' => '001', 'concentration' => '1 µg/µl', 'reactivity' => '<em>Acidaminococcus sp.</em>', 'type' => 'Monoclonal', 'purity' => 'Protein G purified monoclonal antibody', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Western blotting</td> <td>1:1,000</td> <td>Fig 1</td> </tr> </tbody> </table>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => 'TBS containing 0.01 % Na-azide', 'precautions' => 'This product is for research use only. 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Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µg', 'catalog_number' => 'C15200234-100', 'old_catalog_number' => '', 'sf_code' => 'C15200234-D001-000526', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '555', 'price_USD' => '580', 'price_GBP' => '500', 'price_JPY' => '86940', 'price_CNY' => '0', 'price_AUD' => '1450', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 6, 2018', 'slug' => 'acidaminococcus-sp-crisprcpf1-monoclonal-antibody-100-ul', 'meta_title' => 'Acidaminococcus sp. CRISPR/Cpf1 Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Acidaminococcus sp. CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.', 'modified' => '2022-01-05 15:13:27', 'created' => '2018-02-22 15:55:49' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '123', 'position' => '4', 'parent_id' => '100', 'name' => 'CRISPR/Cpf1 antibodies', 'description' => '<p>We are proud to offer the first antibodies specifically recognizing the Cpf1 nuclease, described as a potential candidate for genome editing in mammalian cells.</p> <h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2> <ul> <li>Raised against the Cpf1 nuclease from <em>Acidaminococcus sp.</em></li> <li>Ideal for Western blot</li> </ul> <div class="row"> <div class="small-3 medium-3 large-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig1.png" /></div> <div class="small-9 medium-9 large-9 columns"> <p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p> </div> </div> <h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2> <ul> <li>Raised against the Cpf1 nuclease from <em>Lachnospiraceae bacterium</em></li> <li>Validated in Western blot, <g class="gr_ gr_22 gr-alert gr_gramm gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="22" data-gr-id="22">immunoprecipitation</g> and immunofluorescence</li> </ul> <div class="row"> <div class="small-5 medium-5 large-5 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig2.png" /></div> <div class="small-7 medium-7 large-7 columns"> <p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4°C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). 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CRISPR/Cpf1 Monoclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Acidaminococcus sp. CRISPR/Cpf1 Monoclonal Antibody validated in WB. Batch-specific data available on the website. Sample size available.', 'modified' => '2022-01-05 15:14:01', 'created' => '2018-02-21 11:15:48', 'ProductsGroup' => array( 'id' => '271', 'product_id' => '2953', 'group_id' => '244' ) ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'locale' => 'eng' ) $description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>' $name = 'WB' $document = array( 'id' => '993', 'name' => 'Acidaminococcus sp. CRISPR/Cpf1 monoclonal antibody', 'description' => '<p>Monoclonal antibody raised in mouse against Acidaminococcus sp. 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CRISPR Cpf1 antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Acidaminococcus/SDS-C15200234-Acidaminococcus_sp_CRISPR_Cpf1_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2021-01-26 13:21:20', 'created' => '2021-01-26 13:21:20', 'ProductsSafetySheet' => array( 'id' => '1992', 'product_id' => '2954', 'safety_sheet_id' => '1132' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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