ACTL6B Antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

Antibody portal

Antibodies

Catalog Number

Format

Price

C15310119
(CS-119-100)

100 µl

$260.00
Bulk order

Alternative names: ACTL6, BAF53B

Polyclonal antibody raised in rabbit against human ACTL6B (actin-like 6B), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein.

Lot	A514-004
Concentration	not determined
Species reactivity	Human
Type	Polyclonal
Purity	Whole antiserum
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:500 - 1:1,000	Fig 1
Western Blotting	1:1,000	Fig 2

Batch-Specific Validation Data

Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against ACTL6B (Cat. No. CS-119-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:76,000.

Figure 2. Western blot analysis using the Diagenode antibody directed against ACTL6B
Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against ACTL6B (Cat. No. CS-119-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 47 kDa) is indicated on the right; the marker (in kDa) is shown on the left.

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$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
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	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'in_menu' => false,
	'online' => true,
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	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'ProductsApplication' => array(
		'id' => '1939',
		'product_id' => '2109',
		'application_id' => '19'
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	(int) 0 => 'western-blot-antibodies'
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	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'created' => '2015-01-07 09:20:00',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
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	'name' => 'Epigenetic Antibodies Brochure',
	'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
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	'meta_description' => '',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
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	'meta_keywords' => '',
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			'antibody_id' => '150',
			'name' => 'ACTL6B Antibody ',
			'description' => '<p><span>Alternative names: <strong>ACTL6</strong>, <strong>BAF53B</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human ACTL6B (actin-like 6B), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein.</span></p>',
			'label1' => 'Batch-Specific Validation Data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310119_fig1.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" width="319" height="251" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against ACTL6B (Cat. No. CS-119-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:76,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310119_fig2.jpg" alt="Western blot" width="134" height="126" /></p>
</div>
<div class="small-9 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ACTL6B</strong><br />Nuclear extracts of HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against ACTL6B (Cat. No. CS-119-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 47 kDa) is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>',
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			'label3' => '',
			'info3' => '',
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			'old_catalog_number' => 'CS-119-100',
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			'type' => 'FRE',
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			'price_EUR' => '260',
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			'meta_description' => 'ACTL6B (actin-like 6B) Polyclonal Antibody  validated  in WB and ELISA.  Batch-specific data available on the website. Alternative names: ACTL6, BAF53B',
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			'created' => '2015-06-29 14:08:20',
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<tr>
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<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:500 - 1:1,000</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
</tr>
</tbody>
</table>',
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			'storage_buffer' => '',
			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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			'slug' => '',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2016-08-03 11:31:42',
			'created' => '0000-00-00 00:00:00',
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$meta_description = 'ACTL6B (actin-like 6B) Polyclonal Antibody  validated  in WB and ELISA.  Batch-specific data available on the website. Alternative names: ACTL6, BAF53B'
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		'name' => 'ACTL6B Antibody ',
		'description' => '<p><span>Alternative names: <strong>ACTL6</strong>, <strong>BAF53B</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against human ACTL6B (actin-like 6B), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein.</span></p>',
		'label1' => 'Batch-Specific Validation Data',
		'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310119_fig1.jpg" alt="ELISA" style="display: block; margin-left: auto; margin-right: auto;" width="319" height="251" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against ACTL6B (Cat. No. CS-119-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:76,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310119_fig2.jpg" alt="Western blot" width="134" height="126" /></p>
</div>
<div class="small-9 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ACTL6B</strong><br />Nuclear extracts of HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against ACTL6B (Cat. No. CS-119-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 47 kDa) is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>',
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		'slug' => 'actl6b-polyclonal-antibody-pioneer-100-ul',
		'meta_title' => 'ACTL6B Polyclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'ACTL6B (actin-like 6B) Polyclonal Antibody  validated  in WB and ELISA.  Batch-specific data available on the website. Alternative names: ACTL6, BAF53B',
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		'host' => '*****',
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		'name' => 'ACTL6B polyclonal antibody',
		'description' => 'ACTL6B (UniProt/Swiss-Prot entry O94805) belongs to the family of actin-related proteins (ARPs) which are involved in diverse cellular processes such as vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. ACTL6B is a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is thought to antagonize chromatin-mediated transcriptional repression thereby facilitating transcriptional activation of specific genes. Further, ACTL6B may be involved in gene regulation by structural modulation of their chromatin, specifically in the brain.',
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		'isotype' => '',
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<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ELISA</td>
<td>1:500 - 1:1,000</td>
<td>Fig 1</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 2</td>
</tr>
</tbody>
</table>',
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		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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		'slug' => '',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2016-08-03 11:31:42',
		'created' => '0000-00-00 00:00:00',
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<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
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			'online' => true,
			'tabular' => true,
			'slug' => 'elisa-antibodies',
			'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody',
			'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications',
			'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
			'modified' => '2016-01-13 12:21:41',
			'created' => '2014-07-08 08:13:28',
			'ProductsApplication' => array(
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			'position' => '10',
			'parent_id' => '40',
			'name' => 'WB',
			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'tabular' => true,
			'slug' => 'western-blot-antibodies',
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			'created' => '2015-01-07 09:20:00',
			'ProductsApplication' => array(
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins',
			'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode',
			'modified' => '2019-07-04 16:19:01',
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			'ProductsCategory' => array(
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			'CookiesTag' => array([maximum depth reached])
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			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'created' => '2015-11-02 14:49:22',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ACTL6B</strong><br />Nuclear extracts of HeLa cells (40 &mu;g) were analysed by Western blot using the Diagenode antibody against ACTL6B (Cat. No. CS-119-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 47 kDa) is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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Datasheet ACTL6B CS-119-100 DATASHEET Datasheet description	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

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