AML-ETO Antibody

Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against AML-ETO (Cat. No. pAb-080-050), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.

Figure 2. Western blot analysis using the Diagenode antibody directed against AML-ETO
Figure 2A:  Schematic representation of the construction of GST-fusion proteins containing different parts of AML-ETO were constructed and run on a 15% polyacrylamide gel.
Figure 2B: Western blot was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein.
Figure 2C: Western blot was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. On the left, a molecular weight marker is shown (in kDa). The location of AML-ETO and a presumed splice variant (missing 106 C-terminal amino acids) are indicated on the right.