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Alternative names: AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2
Polyclonal antibody raised in rabbit against the AML-ETO (RUNX1) fusion protein, using 3 different KLH-conjugated synthetic peptides. The antibody recognizes the ETO (RUNX1T1) part of the fusion protein.
| Lot | A134-0011 |
|---|---|
| Concentration | 0.2 µg/µl |
| Species reactivity | Human, mouse |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2 |
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against AML-ETO (Cat. No. pAb-080-050), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.
Figure 2. Western blot analysis using the Diagenode antibody directed against AML-ETO
Figure 2A: Schematic representation of the construction of GST-fusion proteins containing different parts of AML-ETO were constructed and run on a 15% polyacrylamide gel.
Figure 2B: Western blot was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein.
Figure 2C: Western blot was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. On the left, a molecular weight marker is shown (in kDa). The location of AML-ETO and a presumed splice variant (missing 106 C-terminal amino acids) are indicated on the right.
| ELISA Enzyme-linked immunosorbent assay. Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| Datasheet AML-ETO pAb-080-050 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: AML-ETO Antibody (Diagenode Cat# C15410080 Lot# A134-0011). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Esculetin Downregulates the Expression of AML1-ETO and C-Kit in Kasumi-1 Cell Line by Decreasing Half-Life of mRNA |
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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against AML-ETO (Cat. No. pAb-080-050), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410080_fig2.jpg" alt="Western blot" height="391" width="292" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against AML-ETO </strong><br /><strong>Figure 2A:</strong> Schematic representation of the construction of GST-fusion proteins containing different parts of AML-ETO were constructed and run on a 15% polyacrylamide gel.<br /><strong>Figure 2B:</strong> Western blot was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein. <br /><strong>Figure 2C:</strong> Western blot was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the Diagenode antibody against AML-ETO (Cat. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:25:10', 'created' => '0000-00-00 00:00:00', 'select_label' => '144 - AML-ETO polyclonal antibody (A134-0011 - 0.2 µg/µl - Human, mouse - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'en' $meta_keywords = '' $meta_description = 'AML-ETO Polyclonal Antibody validated in WB and ELISA. 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Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against AML-ETO (Cat. No. pAb-080-050), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410080_fig2.jpg" alt="Western blot" height="391" width="292" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against AML-ETO </strong><br /><strong>Figure 2A:</strong> Schematic representation of the construction of GST-fusion proteins containing different parts of AML-ETO were constructed and run on a 15% polyacrylamide gel.<br /><strong>Figure 2B:</strong> Western blot was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein. <br /><strong>Figure 2C:</strong> Western blot was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. On the left, a molecular weight marker is shown (in kDa). 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ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. 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All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2618', 'name' => 'Esculetin Downregulates the Expression of AML1-ETO and C-Kit in Kasumi-1 Cell Line by Decreasing Half-Life of mRNA', 'authors' => 'Sawney S, Arora R, Aggarwal KK, Saluja D', 'description' => 'One of the most frequent genetic aberrations in acute myeloid leukemia (AML) is chromosomal translocation between AML1/RUNX1 on chromosome 21 and ETO gene on chromosome 8 resulting in the expression of chimeric oncogene AML1-ETO. Although patients with t(8;21) translocation have good prognosis, 5-year survival is observed only in 50% of the cases. AML1-ETO translocation is usually accompanied by overexpression of mutant C-Kit, a tyrosine kinase, which contributes to uncontrolled proliferation of premature blood cells leading to relapse and poor prognosis. We illustrate the potential use of esculetin on leukemic cell line, Kasumi-1, bearing t(8;21) translocation and mutated C-Kit gene. Esculetin decreases the expression of AML1-ETO at both protein and transcript level within 24 hours of treatment. Half-life of AML1-ETO mRNA was reduced from 7 hours to 1.5 hours. Similarly half-life of C-Kit mRNA was reduced to 2 hours from 5 hours in esculetin treated cells. Esculetin also perturbed the expression of ectopically expressed AML1-ETO in U937 cells. The decreased expression of AML1-ETO chimeric gene was associated with increased expression of LAT1 and RUNX3 genes, targets of AML1. We envisage that discovery of a drug candidate which could target both these mutated genes would be a considerable breakthrough for future application.', 'date' => '0000-00-00', 'pmid' => 'http://www.hindawi.com/journals/jo/2015/781473/abs/', 'doi' => '', 'modified' => '2015-07-24 15:39:05', 'created' => '2015-07-24 15:39:05', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(pAb-080-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'locale' => 'eng' ) $description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( 'id' => '1829', 'product_id' => '2238', 'document_id' => '38' ) ) $publication = array( 'id' => '2618', 'name' => 'Esculetin Downregulates the Expression of AML1-ETO and C-Kit in Kasumi-1 Cell Line by Decreasing Half-Life of mRNA', 'authors' => 'Sawney S, Arora R, Aggarwal KK, Saluja D', 'description' => 'One of the most frequent genetic aberrations in acute myeloid leukemia (AML) is chromosomal translocation between AML1/RUNX1 on chromosome 21 and ETO gene on chromosome 8 resulting in the expression of chimeric oncogene AML1-ETO. Although patients with t(8;21) translocation have good prognosis, 5-year survival is observed only in 50% of the cases. AML1-ETO translocation is usually accompanied by overexpression of mutant C-Kit, a tyrosine kinase, which contributes to uncontrolled proliferation of premature blood cells leading to relapse and poor prognosis. We illustrate the potential use of esculetin on leukemic cell line, Kasumi-1, bearing t(8;21) translocation and mutated C-Kit gene. Esculetin decreases the expression of AML1-ETO at both protein and transcript level within 24 hours of treatment. Half-life of AML1-ETO mRNA was reduced from 7 hours to 1.5 hours. Similarly half-life of C-Kit mRNA was reduced to 2 hours from 5 hours in esculetin treated cells. Esculetin also perturbed the expression of ectopically expressed AML1-ETO in U937 cells. The decreased expression of AML1-ETO chimeric gene was associated with increased expression of LAT1 and RUNX3 genes, targets of AML1. We envisage that discovery of a drug candidate which could target both these mutated genes would be a considerable breakthrough for future application.', 'date' => '0000-00-00', 'pmid' => 'http://www.hindawi.com/journals/jo/2015/781473/abs/', 'doi' => '', 'modified' => '2015-07-24 15:39:05', 'created' => '2015-07-24 15:39:05', 'ProductsPublication' => array( 'id' => '768', 'product_id' => '2238', 'publication_id' => '2618' ) ) $externalLink = ' <a href="http://www.hindawi.com/journals/jo/2015/781473/abs/" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'AML-ETO Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2', 'meta_title' => 'AML-ETO Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2238', 'antibody_id' => '144', 'name' => 'AML-ETO Antibody ', 'description' => '<p><span>Alternative names: <strong>AML1T1</strong>, <strong>CBFA2T1</strong>, <strong>CDR</strong>, <strong>ETO</strong>, <strong>MTG8</strong>, <strong>ZMYND2</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against the AML-ETO (RUNX1) fusion protein, using 3 different KLH-conjugated synthetic peptides. The antibody recognizes the ETO (RUNX1T1) part of the fusion protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410080_fig1.jpg" alt="ELISA" height="347" width="368" /><br /><br /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against AML-ETO (Cat. No. pAb-080-050), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410080_fig2.jpg" alt="Western blot" height="391" width="292" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against AML-ETO </strong><br /><strong>Figure 2A:</strong> Schematic representation of the construction of GST-fusion proteins containing different parts of AML-ETO were constructed and run on a 15% polyacrylamide gel.<br /><strong>Figure 2B:</strong> Western blot was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein. <br /><strong>Figure 2C:</strong> Western blot was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. On the left, a molecular weight marker is shown (in kDa). The location of AML-ETO and a presumed splice variant (missing 106 C-terminal amino acids) are indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/250 µl', 'catalog_number' => 'C15410080', 'old_catalog_number' => 'pAb-080-050', 'sf_code' => 'C15410080-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'aml-eto-polyclonal-antibody-pioneer-50-ug-250-ul', 'meta_title' => 'AML-ETO Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AML-ETO Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2', 'modified' => '2022-01-05 15:17:51', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '144', 'name' => 'AML-ETO polyclonal antibody', 'description' => 'RUNX1T1 (UniProt/Swiss-Prot entry Q06455) is a putative transcription factor which forms a heterodimer with CBFA2T3. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:25:10', 'created' => '0000-00-00 00:00:00', 'select_label' => '144 - AML-ETO polyclonal antibody (A134-0011 - 0.2 µg/µl - Human, mouse - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'en' $meta_keywords = '' $meta_description = 'AML-ETO Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2' $meta_title = 'AML-ETO Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2238', 'antibody_id' => '144', 'name' => 'AML-ETO Antibody ', 'description' => '<p><span>Alternative names: <strong>AML1T1</strong>, <strong>CBFA2T1</strong>, <strong>CDR</strong>, <strong>ETO</strong>, <strong>MTG8</strong>, <strong>ZMYND2</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against the AML-ETO (RUNX1) fusion protein, using 3 different KLH-conjugated synthetic peptides. The antibody recognizes the ETO (RUNX1T1) part of the fusion protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410080_fig1.jpg" alt="ELISA" height="347" width="368" /><br /><br /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against AML-ETO (Cat. No. pAb-080-050), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410080_fig2.jpg" alt="Western blot" height="391" width="292" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against AML-ETO </strong><br /><strong>Figure 2A:</strong> Schematic representation of the construction of GST-fusion proteins containing different parts of AML-ETO were constructed and run on a 15% polyacrylamide gel.<br /><strong>Figure 2B:</strong> Western blot was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein. <br /><strong>Figure 2C:</strong> Western blot was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. On the left, a molecular weight marker is shown (in kDa). 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Batch-specific data available on the website. Alternative names: AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2', 'modified' => '2022-01-05 15:17:51', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '144', 'name' => 'AML-ETO polyclonal antibody', 'description' => 'RUNX1T1 (UniProt/Swiss-Prot entry Q06455) is a putative transcription factor which forms a heterodimer with CBFA2T3. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:25:10', 'created' => '0000-00-00 00:00:00', 'select_label' => '144 - AML-ETO polyclonal antibody (A134-0011 - 0.2 µg/µl - Human, mouse - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '493', 'name' => 'Datasheet AML-ETO pAb-080-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_AML-ETO_pAb-080-050.pdf', 'slug' => 'datasheet-aml-eto-pab-080-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. 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All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2618', 'name' => 'Esculetin Downregulates the Expression of AML1-ETO and C-Kit in Kasumi-1 Cell Line by Decreasing Half-Life of mRNA', 'authors' => 'Sawney S, Arora R, Aggarwal KK, Saluja D', 'description' => 'One of the most frequent genetic aberrations in acute myeloid leukemia (AML) is chromosomal translocation between AML1/RUNX1 on chromosome 21 and ETO gene on chromosome 8 resulting in the expression of chimeric oncogene AML1-ETO. Although patients with t(8;21) translocation have good prognosis, 5-year survival is observed only in 50% of the cases. AML1-ETO translocation is usually accompanied by overexpression of mutant C-Kit, a tyrosine kinase, which contributes to uncontrolled proliferation of premature blood cells leading to relapse and poor prognosis. We illustrate the potential use of esculetin on leukemic cell line, Kasumi-1, bearing t(8;21) translocation and mutated C-Kit gene. Esculetin decreases the expression of AML1-ETO at both protein and transcript level within 24 hours of treatment. Half-life of AML1-ETO mRNA was reduced from 7 hours to 1.5 hours. Similarly half-life of C-Kit mRNA was reduced to 2 hours from 5 hours in esculetin treated cells. Esculetin also perturbed the expression of ectopically expressed AML1-ETO in U937 cells. The decreased expression of AML1-ETO chimeric gene was associated with increased expression of LAT1 and RUNX3 genes, targets of AML1. We envisage that discovery of a drug candidate which could target both these mutated genes would be a considerable breakthrough for future application.', 'date' => '0000-00-00', 'pmid' => 'http://www.hindawi.com/journals/jo/2015/781473/abs/', 'doi' => '', 'modified' => '2015-07-24 15:39:05', 'created' => '2015-07-24 15:39:05', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(pAb-080-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'locale' => 'eng' ) $description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>' $name = 'WB' $document = array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. 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Although patients with t(8;21) translocation have good prognosis, 5-year survival is observed only in 50% of the cases. AML1-ETO translocation is usually accompanied by overexpression of mutant C-Kit, a tyrosine kinase, which contributes to uncontrolled proliferation of premature blood cells leading to relapse and poor prognosis. We illustrate the potential use of esculetin on leukemic cell line, Kasumi-1, bearing t(8;21) translocation and mutated C-Kit gene. Esculetin decreases the expression of AML1-ETO at both protein and transcript level within 24 hours of treatment. Half-life of AML1-ETO mRNA was reduced from 7 hours to 1.5 hours. Similarly half-life of C-Kit mRNA was reduced to 2 hours from 5 hours in esculetin treated cells. Esculetin also perturbed the expression of ectopically expressed AML1-ETO in U937 cells. The decreased expression of AML1-ETO chimeric gene was associated with increased expression of LAT1 and RUNX3 genes, targets of AML1. We envisage that discovery of a drug candidate which could target both these mutated genes would be a considerable breakthrough for future application.', 'date' => '0000-00-00', 'pmid' => 'http://www.hindawi.com/journals/jo/2015/781473/abs/', 'doi' => '', 'modified' => '2015-07-24 15:39:05', 'created' => '2015-07-24 15:39:05', 'ProductsPublication' => array( 'id' => '768', 'product_id' => '2238', 'publication_id' => '2618' ) ) $externalLink = ' <a href="http://www.hindawi.com/journals/jo/2015/781473/abs/" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'AML-ETO Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2', 'meta_title' => 'AML-ETO Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2238', 'antibody_id' => '144', 'name' => 'AML-ETO Antibody ', 'description' => '<p><span>Alternative names: <strong>AML1T1</strong>, <strong>CBFA2T1</strong>, <strong>CDR</strong>, <strong>ETO</strong>, <strong>MTG8</strong>, <strong>ZMYND2</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against the AML-ETO (RUNX1) fusion protein, using 3 different KLH-conjugated synthetic peptides. The antibody recognizes the ETO (RUNX1T1) part of the fusion protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410080_fig1.jpg" alt="ELISA" height="347" width="368" /><br /><br /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against AML-ETO (Cat. No. pAb-080-050), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410080_fig2.jpg" alt="Western blot" height="391" width="292" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against AML-ETO </strong><br /><strong>Figure 2A:</strong> Schematic representation of the construction of GST-fusion proteins containing different parts of AML-ETO were constructed and run on a 15% polyacrylamide gel.<br /><strong>Figure 2B:</strong> Western blot was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein. <br /><strong>Figure 2C:</strong> Western blot was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. On the left, a molecular weight marker is shown (in kDa). The location of AML-ETO and a presumed splice variant (missing 106 C-terminal amino acids) are indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/250 µl', 'catalog_number' => 'C15410080', 'old_catalog_number' => 'pAb-080-050', 'sf_code' => 'C15410080-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'aml-eto-polyclonal-antibody-pioneer-50-ug-250-ul', 'meta_title' => 'AML-ETO Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AML-ETO Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2', 'modified' => '2022-01-05 15:17:51', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '144', 'name' => 'AML-ETO polyclonal antibody', 'description' => 'RUNX1T1 (UniProt/Swiss-Prot entry Q06455) is a putative transcription factor which forms a heterodimer with CBFA2T3. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:25:10', 'created' => '0000-00-00 00:00:00', 'select_label' => '144 - AML-ETO polyclonal antibody (A134-0011 - 0.2 µg/µl - Human, mouse - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'en' $meta_keywords = '' $meta_description = 'AML-ETO Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2' $meta_title = 'AML-ETO Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2238', 'antibody_id' => '144', 'name' => 'AML-ETO Antibody ', 'description' => '<p><span>Alternative names: <strong>AML1T1</strong>, <strong>CBFA2T1</strong>, <strong>CDR</strong>, <strong>ETO</strong>, <strong>MTG8</strong>, <strong>ZMYND2</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against the AML-ETO (RUNX1) fusion protein, using 3 different KLH-conjugated synthetic peptides. The antibody recognizes the ETO (RUNX1T1) part of the fusion protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410080_fig1.jpg" alt="ELISA" height="347" width="368" /><br /><br /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against AML-ETO (Cat. No. pAb-080-050), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410080_fig2.jpg" alt="Western blot" height="391" width="292" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against AML-ETO </strong><br /><strong>Figure 2A:</strong> Schematic representation of the construction of GST-fusion proteins containing different parts of AML-ETO were constructed and run on a 15% polyacrylamide gel.<br /><strong>Figure 2B:</strong> Western blot was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein. <br /><strong>Figure 2C:</strong> Western blot was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. On the left, a molecular weight marker is shown (in kDa). 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:25:10', 'created' => '0000-00-00 00:00:00', 'select_label' => '144 - AML-ETO polyclonal antibody (A134-0011 - 0.2 µg/µl - Human, mouse - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '493', 'name' => 'Datasheet AML-ETO pAb-080-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_AML-ETO_pAb-080-050.pdf', 'slug' => 'datasheet-aml-eto-pab-080-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2618', 'name' => 'Esculetin Downregulates the Expression of AML1-ETO and C-Kit in Kasumi-1 Cell Line by Decreasing Half-Life of mRNA', 'authors' => 'Sawney S, Arora R, Aggarwal KK, Saluja D', 'description' => 'One of the most frequent genetic aberrations in acute myeloid leukemia (AML) is chromosomal translocation between AML1/RUNX1 on chromosome 21 and ETO gene on chromosome 8 resulting in the expression of chimeric oncogene AML1-ETO. Although patients with t(8;21) translocation have good prognosis, 5-year survival is observed only in 50% of the cases. AML1-ETO translocation is usually accompanied by overexpression of mutant C-Kit, a tyrosine kinase, which contributes to uncontrolled proliferation of premature blood cells leading to relapse and poor prognosis. We illustrate the potential use of esculetin on leukemic cell line, Kasumi-1, bearing t(8;21) translocation and mutated C-Kit gene. Esculetin decreases the expression of AML1-ETO at both protein and transcript level within 24 hours of treatment. Half-life of AML1-ETO mRNA was reduced from 7 hours to 1.5 hours. Similarly half-life of C-Kit mRNA was reduced to 2 hours from 5 hours in esculetin treated cells. Esculetin also perturbed the expression of ectopically expressed AML1-ETO in U937 cells. The decreased expression of AML1-ETO chimeric gene was associated with increased expression of LAT1 and RUNX3 genes, targets of AML1. We envisage that discovery of a drug candidate which could target both these mutated genes would be a considerable breakthrough for future application.', 'date' => '0000-00-00', 'pmid' => 'http://www.hindawi.com/journals/jo/2015/781473/abs/', 'doi' => '', 'modified' => '2015-07-24 15:39:05', 'created' => '2015-07-24 15:39:05', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(pAb-080-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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Although patients with t(8;21) translocation have good prognosis, 5-year survival is observed only in 50% of the cases. AML1-ETO translocation is usually accompanied by overexpression of mutant C-Kit, a tyrosine kinase, which contributes to uncontrolled proliferation of premature blood cells leading to relapse and poor prognosis. We illustrate the potential use of esculetin on leukemic cell line, Kasumi-1, bearing t(8;21) translocation and mutated C-Kit gene. Esculetin decreases the expression of AML1-ETO at both protein and transcript level within 24 hours of treatment. Half-life of AML1-ETO mRNA was reduced from 7 hours to 1.5 hours. Similarly half-life of C-Kit mRNA was reduced to 2 hours from 5 hours in esculetin treated cells. Esculetin also perturbed the expression of ectopically expressed AML1-ETO in U937 cells. The decreased expression of AML1-ETO chimeric gene was associated with increased expression of LAT1 and RUNX3 genes, targets of AML1. We envisage that discovery of a drug candidate which could target both these mutated genes would be a considerable breakthrough for future application.', 'date' => '0000-00-00', 'pmid' => 'http://www.hindawi.com/journals/jo/2015/781473/abs/', 'doi' => '', 'modified' => '2015-07-24 15:39:05', 'created' => '2015-07-24 15:39:05', 'ProductsPublication' => array( 'id' => '768', 'product_id' => '2238', 'publication_id' => '2618' ) ) $externalLink = ' <a href="http://www.hindawi.com/journals/jo/2015/781473/abs/" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'AML-ETO Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2', 'meta_title' => 'AML-ETO Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2238', 'antibody_id' => '144', 'name' => 'AML-ETO Antibody ', 'description' => '<p><span>Alternative names: <strong>AML1T1</strong>, <strong>CBFA2T1</strong>, <strong>CDR</strong>, <strong>ETO</strong>, <strong>MTG8</strong>, <strong>ZMYND2</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against the AML-ETO (RUNX1) fusion protein, using 3 different KLH-conjugated synthetic peptides. The antibody recognizes the ETO (RUNX1T1) part of the fusion protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410080_fig1.jpg" alt="ELISA" height="347" width="368" /><br /><br /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against AML-ETO (Cat. No. pAb-080-050), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410080_fig2.jpg" alt="Western blot" height="391" width="292" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against AML-ETO </strong><br /><strong>Figure 2A:</strong> Schematic representation of the construction of GST-fusion proteins containing different parts of AML-ETO were constructed and run on a 15% polyacrylamide gel.<br /><strong>Figure 2B:</strong> Western blot was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein. <br /><strong>Figure 2C:</strong> Western blot was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. On the left, a molecular weight marker is shown (in kDa). The location of AML-ETO and a presumed splice variant (missing 106 C-terminal amino acids) are indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/250 µl', 'catalog_number' => 'C15410080', 'old_catalog_number' => 'pAb-080-050', 'sf_code' => 'C15410080-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'aml-eto-polyclonal-antibody-pioneer-50-ug-250-ul', 'meta_title' => 'AML-ETO Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AML-ETO Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2', 'modified' => '2022-01-05 15:17:51', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '144', 'name' => 'AML-ETO polyclonal antibody', 'description' => 'RUNX1T1 (UniProt/Swiss-Prot entry Q06455) is a putative transcription factor which forms a heterodimer with CBFA2T3. Defects in RUNX1T1 have been associated with acute myeloid leukemia (AML-M2) and may be a cause of colorectal cancer.', 'clonality' => '', 'isotype' => '', 'lot' => 'A134-0011', 'concentration' => '0.2 µg/µl', 'reactivity' => 'Human, mouse', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 11:25:10', 'created' => '0000-00-00 00:00:00', 'select_label' => '144 - AML-ETO polyclonal antibody (A134-0011 - 0.2 µg/µl - Human, mouse - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'en' $meta_keywords = '' $meta_description = 'AML-ETO Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2' $meta_title = 'AML-ETO Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2238', 'antibody_id' => '144', 'name' => 'AML-ETO Antibody ', 'description' => '<p><span>Alternative names: <strong>AML1T1</strong>, <strong>CBFA2T1</strong>, <strong>CDR</strong>, <strong>ETO</strong>, <strong>MTG8</strong>, <strong>ZMYND2</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against the AML-ETO (RUNX1) fusion protein, using 3 different KLH-conjugated synthetic peptides. The antibody recognizes the ETO (RUNX1T1) part of the fusion protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410080_fig1.jpg" alt="ELISA" height="347" width="368" /><br /><br /></p> </div> <div class="small-7 columns"> <p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against AML-ETO (Cat. No. pAb-080-050), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.</small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410080_fig2.jpg" alt="Western blot" height="391" width="292" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against AML-ETO </strong><br /><strong>Figure 2A:</strong> Schematic representation of the construction of GST-fusion proteins containing different parts of AML-ETO were constructed and run on a 15% polyacrylamide gel.<br /><strong>Figure 2B:</strong> Western blot was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein. <br /><strong>Figure 2C:</strong> Western blot was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the Diagenode antibody against AML-ETO (Cat. No. pAb-080-050), diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. On the left, a molecular weight marker is shown (in kDa). The location of AML-ETO and a presumed splice variant (missing 106 C-terminal amino acids) are indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µg/250 µl', 'catalog_number' => 'C15410080', 'old_catalog_number' => 'pAb-080-050', 'sf_code' => 'C15410080-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'aml-eto-polyclonal-antibody-pioneer-50-ug-250-ul', 'meta_title' => 'AML-ETO Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'AML-ETO Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website. Alternative names: AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2', 'modified' => '2022-01-05 15:17:51', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '144', 'name' => 'AML-ETO polyclonal antibody', 'description' => 'RUNX1T1 (UniProt/Swiss-Prot entry Q06455) is a putative transcription factor which forms a heterodimer with CBFA2T3. Defects in RUNX1T1 have been associated with acute myeloid leukemia (AML-M2) and may be a cause of colorectal cancer.', 'clonality' => '', 'isotype' => '', 'lot' => 'A134-0011', 'concentration' => '0.2 µg/µl', 'reactivity' => 'Human, mouse', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:100</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000</td> <td>Fig 2</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '493', 'name' => 'Datasheet AML-ETO pAb-080-050', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_AML-ETO_pAb-080-050.pdf', 'slug' => 'datasheet-aml-eto-pab-080-050', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. 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Although patients with t(8;21) translocation have good prognosis, 5-year survival is observed only in 50% of the cases. AML1-ETO translocation is usually accompanied by overexpression of mutant C-Kit, a tyrosine kinase, which contributes to uncontrolled proliferation of premature blood cells leading to relapse and poor prognosis. We illustrate the potential use of esculetin on leukemic cell line, Kasumi-1, bearing t(8;21) translocation and mutated C-Kit gene. Esculetin decreases the expression of AML1-ETO at both protein and transcript level within 24 hours of treatment. Half-life of AML1-ETO mRNA was reduced from 7 hours to 1.5 hours. Similarly half-life of C-Kit mRNA was reduced to 2 hours from 5 hours in esculetin treated cells. Esculetin also perturbed the expression of ectopically expressed AML1-ETO in U937 cells. The decreased expression of AML1-ETO chimeric gene was associated with increased expression of LAT1 and RUNX3 genes, targets of AML1. We envisage that discovery of a drug candidate which could target both these mutated genes would be a considerable breakthrough for future application.', 'date' => '0000-00-00', 'pmid' => 'http://www.hindawi.com/journals/jo/2015/781473/abs/', 'doi' => '', 'modified' => '2015-07-24 15:39:05', 'created' => '2015-07-24 15:39:05', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(pAb-080-050)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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Although patients with t(8;21) translocation have good prognosis, 5-year survival is observed only in 50% of the cases. AML1-ETO translocation is usually accompanied by overexpression of mutant C-Kit, a tyrosine kinase, which contributes to uncontrolled proliferation of premature blood cells leading to relapse and poor prognosis. We illustrate the potential use of esculetin on leukemic cell line, Kasumi-1, bearing t(8;21) translocation and mutated C-Kit gene. Esculetin decreases the expression of AML1-ETO at both protein and transcript level within 24 hours of treatment. Half-life of AML1-ETO mRNA was reduced from 7 hours to 1.5 hours. Similarly half-life of C-Kit mRNA was reduced to 2 hours from 5 hours in esculetin treated cells. Esculetin also perturbed the expression of ectopically expressed AML1-ETO in U937 cells. The decreased expression of AML1-ETO chimeric gene was associated with increased expression of LAT1 and RUNX3 genes, targets of AML1. 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