Antibody package for CUT&Tag (anti-mouse)

Catalog Number

Format

Price

C01070027

8 rxns

$90.00

Other format

C01070023

24 rxns

Diagenode’s Antibody Package for CUT&Tag (anti-mouse) has been designed to be used with the iDeal CUT&Tag for Histones Kit, This package should be used if the primary antibody of interest used in the CUT&Tag assays has been raised in mouse.

The Antibody Package for CUT&Tag (anti-mouse) contains species-specific controls antibodies:

Anti-mouse secondary antibody
Mouse H3K27me3 positive control antibody
Mouse negative IgG control
TSH2B primer pair (human)
GAPDH TSS primer pair (human)

The package contains enough secondary antibody to perform 8 reactions. The positive and negative control antibodies are enough for 2 reactions each.

If the primary antibody of interest used in the CUT&Tag assays has been raised in rabbit, please use the Antibody Package for CUT&Tag (anti-rabbit).

Check out:

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<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">Antibody Package for CUT&amp;Tag (anti-rabbit)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<li>Mouse negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
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<p>The package contains enough secondary antibody to perform&nbsp;8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
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<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-mouse) has been designed to be used with the<a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> iDeal CUT&amp;Tag for Histones Kit</a>, This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse.</p>
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<ul>
<li>Anti-mouse secondary antibody</li>
<li>Mouse H3K27me3 positive control antibody</li>
<li>Mouse negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
</ul>
<p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">Antibody Package for CUT&amp;Tag (anti-rabbit)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><strong>CUT&amp;Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&amp;Tag &ndash; our iDeal CUT&amp;Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein &ndash; pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&amp;Tag</a> proving their high performance in this assay.</p>
<br /> <a href="https://www.diagenode.com/files/application_notes/AN-iDealCUTandTag.pdf"><img src="https://www.diagenode.com/img/banners/cutandtag-appnote.png" /></a><br /><br /></div>
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<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a>
<div id="v5" class="content">
<p>The iDeal CUT&amp;Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode&rsquo;s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode&rsquo;s Primer Indexes for tagmented libraries.</p>
<img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div>
<h2>Products for CUT&amp;Tag assay</h2>
<h3 class="diacol">Complete solutions</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&amp;Tag kit for Histones</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">Fusion protein</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li>
</ul>
<h3 class="diacol">CUT&amp;Tag grade antibodies</h3>
<ul class="nobullet">
<li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&amp;Tag</a></li>
<li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li>
<li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&amp;Tag</a></li>
</ul>
<h3 class="diacol">Positive &amp; Negative CUT&amp;Tag control</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">DNA purification</h3>
<p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p>
<h3 class="diacol">Sequencing indexes</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li>
</ul>
</li>
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	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
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		'antibody_id' => null,
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		'description' => '<p><a href="https://www.diagenode.com/files/products/kits/iDeal-CUTandTag-kit-for-histones-manual.pdf"><img src="https://www.diagenode.com/img/buttons/bt-manual.png" /></a></p>
<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-mouse) has been designed to be used with the<a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> iDeal CUT&amp;Tag for Histones Kit</a>, This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse.</p>
<p>The Antibody Package for CUT&amp;Tag (anti-mouse) contains species-specific controls antibodies:</p>
<ul>
<li>Anti-mouse secondary antibody</li>
<li>Mouse H3K27me3 positive control antibody</li>
<li>Mouse negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
</ul>
<p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">Antibody Package for CUT&amp;Tag (anti-rabbit)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
</ul>',
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-mouse) has been designed to be used with the<a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> iDeal CUT&amp;Tag for Histones Kit</a>, This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse.</p>
<p>The Antibody Package for CUT&amp;Tag (anti-mouse) contains species-specific controls antibodies:</p>
<ul>
<li>Anti-mouse secondary antibody</li>
<li>Mouse H3K27me3 positive control antibody</li>
<li>Mouse negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
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<p>The package contains enough secondary antibody to perform&nbsp;8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">Antibody Package for CUT&amp;Tag (anti-rabbit)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-mouse) has been designed to be used with the<a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> iDeal CUT&amp;Tag for Histones Kit</a>, This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse.</p>
<p>The Antibody Package for CUT&amp;Tag (anti-mouse) contains species-specific controls antibodies:</p>
<ul>
<li>Anti-mouse secondary antibody</li>
<li>Mouse H3K27me3 positive control antibody</li>
<li>Mouse negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
</ul>
<p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">Antibody Package for CUT&amp;Tag (anti-rabbit)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-mouse) has been designed to be used with the<a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> iDeal CUT&amp;Tag for Histones Kit</a>, This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse.</p>
<p>The Antibody Package for CUT&amp;Tag (anti-mouse) contains species-specific controls antibodies:</p>
<ul>
<li>Anti-mouse secondary antibody</li>
<li>Mouse H3K27me3 positive control antibody</li>
<li>Mouse negative IgG control</li>
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<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">Antibody Package for CUT&amp;Tag (anti-rabbit)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-mouse) has been designed to be used with the<a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> iDeal CUT&amp;Tag for Histones Kit</a>, This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse.</p>
<p>The Antibody Package for CUT&amp;Tag (anti-mouse) contains species-specific controls antibodies:</p>
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<li>Anti-mouse secondary antibody</li>
<li>Mouse H3K27me3 positive control antibody</li>
<li>Mouse negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
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<p>The package contains enough secondary antibody to perform&nbsp;8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">Antibody Package for CUT&amp;Tag (anti-rabbit)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-mouse) has been designed to be used with the<a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> iDeal CUT&amp;Tag for Histones Kit</a>, This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse.</p>
<p>The Antibody Package for CUT&amp;Tag (anti-mouse) contains species-specific controls antibodies:</p>
<ul>
<li>Anti-mouse secondary antibody</li>
<li>Mouse H3K27me3 positive control antibody</li>
<li>Mouse negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
</ul>
<p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">Antibody Package for CUT&amp;Tag (anti-rabbit)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p><strong>CUT&amp;Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&amp;Tag &ndash; our iDeal CUT&amp;Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein &ndash; pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&amp;Tag</a> proving their high performance in this assay.</p>
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<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a>
<div id="v5" class="content">
<p>The iDeal CUT&amp;Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode&rsquo;s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode&rsquo;s Primer Indexes for tagmented libraries.</p>
<img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div>
<h2>Products for CUT&amp;Tag assay</h2>
<h3 class="diacol">Complete solutions</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&amp;Tag kit for Histones</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">Fusion protein</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li>
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<h3 class="diacol">CUT&amp;Tag grade antibodies</h3>
<ul class="nobullet">
<li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&amp;Tag</a></li>
<li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li>
<li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&amp;Tag</a></li>
</ul>
<h3 class="diacol">Positive &amp; Negative CUT&amp;Tag control</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">DNA purification</h3>
<p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p>
<h3 class="diacol">Sequencing indexes</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li>
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<p><strong>Check out:</strong></p>
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<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p><strong>Check out:</strong></p>
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<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p><strong>Check out:</strong></p>
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<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
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<p><strong>Check out:</strong></p>
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<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p><strong>CUT&amp;Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&amp;Tag &ndash; our iDeal CUT&amp;Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein &ndash; pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&amp;Tag</a> proving their high performance in this assay.</p>
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<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a>
<div id="v5" class="content">
<p>The iDeal CUT&amp;Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode&rsquo;s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode&rsquo;s Primer Indexes for tagmented libraries.</p>
<img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div>
<h2>Products for CUT&amp;Tag assay</h2>
<h3 class="diacol">Complete solutions</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&amp;Tag kit for Histones</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">Fusion protein</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li>
</ul>
<h3 class="diacol">CUT&amp;Tag grade antibodies</h3>
<ul class="nobullet">
<li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&amp;Tag</a></li>
<li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li>
<li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&amp;Tag</a></li>
</ul>
<h3 class="diacol">Positive &amp; Negative CUT&amp;Tag control</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">DNA purification</h3>
<p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p>
<h3 class="diacol">Sequencing indexes</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li>
</ul>
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<li>Mouse negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
</ul>
<p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">Antibody Package for CUT&amp;Tag (anti-rabbit)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
</ul>',
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	'name' => 'Antibody package for CUT&Tag (anti-mouse)',
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-mouse) has been designed to be used with the<a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> iDeal CUT&amp;Tag for Histones Kit</a>, This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse.</p>
<p>The Antibody Package for CUT&amp;Tag (anti-mouse) contains species-specific controls antibodies:</p>
<ul>
<li>Anti-mouse secondary antibody</li>
<li>Mouse H3K27me3 positive control antibody</li>
<li>Mouse negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
</ul>
<p>The package contains enough secondary antibody to perform&nbsp;8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">Antibody Package for CUT&amp;Tag (anti-rabbit)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
</ul>',
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-mouse) has been designed to be used with the<a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> iDeal CUT&amp;Tag for Histones Kit</a>, This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse.</p>
<p>The Antibody Package for CUT&amp;Tag (anti-mouse) contains species-specific controls antibodies:</p>
<ul>
<li>Anti-mouse secondary antibody</li>
<li>Mouse H3K27me3 positive control antibody</li>
<li>Mouse negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
</ul>
<p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">Antibody Package for CUT&amp;Tag (anti-rabbit)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
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	'meta_keywords' => '',
	'meta_description' => '',
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<p>The Antibody Package for CUT&amp;Tag (anti-mouse) contains species-specific controls antibodies:</p>
<ul>
<li>Anti-mouse secondary antibody</li>
<li>Mouse H3K27me3 positive control antibody</li>
<li>Mouse negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
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<p>The package contains enough secondary antibody to perform&nbsp;8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">Antibody Package for CUT&amp;Tag (anti-rabbit)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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$meta_keywords = ''
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-mouse) has been designed to be used with the<a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> iDeal CUT&amp;Tag for Histones Kit</a>, This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse.</p>
<p>The Antibody Package for CUT&amp;Tag (anti-mouse) contains species-specific controls antibodies:</p>
<ul>
<li>Anti-mouse secondary antibody</li>
<li>Mouse H3K27me3 positive control antibody</li>
<li>Mouse negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
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<p>The package contains enough secondary antibody to perform&nbsp;8 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">Antibody Package for CUT&amp;Tag (anti-rabbit)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
</ul>',
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-mouse) has been designed to be used with the<a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> iDeal CUT&amp;Tag for Histones Kit</a>, This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse.</p>
<p>The Antibody Package for CUT&amp;Tag (anti-mouse) contains species-specific controls antibodies:</p>
<ul>
<li>Anti-mouse secondary antibody</li>
<li>Mouse H3K27me3 positive control antibody</li>
<li>Mouse negative IgG control</li>
<li>TSH2B primer pair (human)</li>
<li>GAPDH TSS primer pair (human)</li>
</ul>
<p>The package contains enough secondary antibody to perform 24 reactions. The positive and negative control antibodies are enough for 2 reactions each.</p>
<p>If the primary antibody of interest used in the CUT&amp;Tag assays has been raised in rabbit, please use the <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24">Antibody Package for CUT&amp;Tag (anti-rabbit)</a>.</p>
<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p><strong>CUT&amp;Tag</strong>-sequencing (<strong>C</strong>leavage <strong>U</strong>nder <strong>T</strong>argets and <strong>Tag</strong>mentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&amp;Tag &ndash; our iDeal CUT&amp;Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein &ndash; pA-Tn5 Transposase. Moreover, we have validated our <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies">ChIP-seq grade antibodies</a> in <a href="https://www.diagenode.com/en/categories/cut-and-tag-antibodies">CUT&amp;Tag</a> proving their high performance in this assay.</p>
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<ul class="accordion" data-accordion="">
<li class="accordion-navigation"><a href="#v5" style="color: #13b29c;"><i class="fa fa-caret-right"></i> How does it work?</a>
<div id="v5" class="content">
<p>The iDeal CUT&amp;Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode&rsquo;s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode&rsquo;s Primer Indexes for tagmented libraries.</p>
<img src="https://www.diagenode.com/img/product/kits/workflow-cutandtag.jpg" /></div>
<h2>Products for CUT&amp;Tag assay</h2>
<h3 class="diacol">Complete solutions</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24" target="_blank">iDeal CUT&amp;Tag kit for Histones</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">Fusion protein</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3064" target="_blank">pA/Tn5 Transposase (loaded)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/products/view/3065" target="_blank">pA/Tn5 Transposase (unloaded)</a></li>
</ul>
<h3 class="diacol">CUT&amp;Tag grade antibodies</h3>
<ul class="nobullet">
<li>Antibodies <a href="https://www.diagenode.com/en/applications/cut-and-tag">validated in CUT&amp;Tag</a></li>
<li>Check out our list of <a href="https://www.diagenode.com/en/categories/chip-seq-grade-antibodies" target="_blank">ChIP-seq grade antibodies</a></li>
<li>Read more about the performance of Diagenode antibodies in <a href="https://www.diagenode.com/en/pages/cut-and-tag" target="_blank">CUT&amp;Tag</a></li>
</ul>
<h3 class="diacol">Positive &amp; Negative CUT&amp;Tag control</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antirabbit-24" target="_blank">Antibody package for CUT&amp;Tag (anti-rabbit)</a></li>
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/p/antibody-package-cut-and-tag-antimouse-24" target="_blank">Antibody package for CUT&amp;Tag (anti-mouse)</a></li>
</ul>
<h3 class="diacol">DNA purification</h3>
<p style="padding-left: 30px;"><a href="https://www.diagenode.com/en/p/ipure-kit-v2-x24">IPure kit v2<br /></a><a href="https://www.diagenode.com/en/p/microchip-diapure-columns-50-rxns">MicroChIP DiaPure columns</a></p>
<h3 class="diacol">Sequencing indexes</h3>
<ul class="nobullet">
<li><i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/en/categories/primer-indexes-for-tagmented-libraries" target="_blank">Primer indexes for tagmented libraries</a></li>
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<p>Diagenode&rsquo;s Antibody Package for CUT&amp;Tag (anti-mouse) has been designed to be used with the<a href="https://www.diagenode.com/en/p/ideal-cut-and-tag-kit-for-histones-24"> iDeal CUT&amp;Tag for Histones Kit</a>, This package should be used if the primary antibody of interest used in the CUT&amp;Tag assays has been raised in mouse.</p>
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<p><strong>Check out:</strong></p>
<ul>
<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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<p><strong>Check out:</strong></p>
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<li><a href="https://www.diagenode.com/en/categories/cutandtag">CUT&amp;Tag</a></li>
<li><a href="https://www.diagenode.com/en/applications/cut-and-tag">CUT&amp;Tag grade antibodies</a></li>
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Chromatin Brochure BROCHURE Whether you are experienced or new to the field of chromatin immunoprecipitation, Diagenode has e...	Download
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