Alternative names: FSRG1, NAT, RING3, RNF3
Polyclonal antibody raised in rabbit against mouse Brd2 (bromodomain containing 2), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the central part of the protein.
| Lot | A355-004 |
|---|---|
| Concentration | not determined |
| Species reactivity | Mouse |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2, (1) |
Figure 1. Determination of the antibody titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Brd2 (Cat. No. CS-094-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against Brd2
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse Brd2 (Cat. No. CS-094-100). The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,000.
 How to properly cite our product/service in your workWe strongly recommend using this: BRD2 Antibody (Hologic Diagenode Cat# C15310094 Lot# A355-004). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
