C2C2 Antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Antibodies

Catalog Number

Format

Price

C15310267-100

100 μl

$580.00
Bulk order

Other format

C15310267-20

20 µl

Polyclonal antibody raised in rabbit against the class 2 type VI CRISPR-Cas effector (C2C2) Leptotrichia shahii using a recombinant protein.

Lot	A2915-004
Concentration	Not determined
Species reactivity	Leptotrichia shahii
Type	Polyclonal
Purity	Whole antiserum from rabbit containing 0.05% azide.
Host	Rabbit
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Western Blotting	1:1,000 - 1/10,000	Fig 1

Validation data

Figure 1. Western blot analysis using the Diagenode antibody directed against C2C2
Western blot was performed on protein extracts from U2OS cells (lane 1) and on extracts from U2OS cells spiked with 50 ng of the immunogen (Lsh C2C2 recombinant protein, lane 2) using the Diagenode antibody against C2C2 (Cat. No. C15310267), diluted 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the C2C2 protein is indicated on the right. It’s unclear what the higher MW signal represents but since it is absent in the lane without the spiked protein, it might be a protein aggregate.

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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310267-wb.jpg" alt="Western blot figure 1" style="display: block; margin-left: auto; margin-right: auto;" width="272" height="350" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against C2C2</strong><br /> Western blot was performed on protein extracts from U2OS cells (lane 1) and on extracts from U2OS cells spiked with 50 ng of the immunogen (Lsh C2C2 recombinant protein, lane 2) using the Diagenode antibody against C2C2 (Cat. No. C15310267), diluted 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the C2C2 protein is indicated on the right. It&rsquo;s unclear what the higher MW signal represents but since it is absent in the lane without the spiked protein, it might be a protein aggregate.</small></p>
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			'modified' => '2024-01-16 13:21:47',
			'created' => '2018-03-05 11:43:07',
			'locale' => 'eng'
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			'name' => 'C2C2 polyclonal antibody',
			'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified in <em>Leptotrichia shahii</em>. This CRISPR system is characterized by the presence of the single effector protein C2C2. C2C2 lacks homology to any known DNA nuclease domain but contains two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and is thought to function solely as an RNA-guided RNA-targeting CRISPR effector.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A2915-004',
			'concentration' => 'Not determined',
			'reactivity' => '<em>Leptotrichia shahii</em>',
			'type' => 'Polyclonal',
			'purity' => 'Whole antiserum from rabbit containing 0.05% azide.',
			'classification' => '',
			'application_table' => '<table>
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<th>Suggested dilution</th>
<th>References</th>
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<td>Western Blotting</td>
<td>1:1,000 - 1/10,000</td>
<td>Fig 1</td>
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			'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
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			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
			'uniprot_acc' => '',
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			'modified' => '2018-03-15 16:14:50',
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			'select_label' => '522 - C2C2 polyclonal antibody (A2915-004 - Not determined - <em>Leptotrichia shahii</em> - Whole antiserum from rabbit containing 0.05% azide. - Rabbit)'
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		'price_GBP' => '120',
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		'last_datasheet_update' => 'January 30, 2018',
		'slug' => 'c2c2-polyclonal-antibody-20-ul',
		'meta_title' => 'C2C2 Polyclonal Antibody | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'C2C2 Polyclonal Antibody validated  in WB. Batch-specific data available on the website. Sample size available.',
		'modified' => '2024-01-16 13:22:07',
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	'name' => 'C2C2 Antibody (sample size)',
	'description' => '',
	'label1' => 'Validation data',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '20 µl',
	'catalog_number' => 'C15310267-20',
	'old_catalog_number' => '',
	'sf_code' => 'C15310267-361',
	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '125',
	'price_USD' => '130',
	'price_GBP' => '120',
	'price_JPY' => '19580',
	'price_CNY' => '0',
	'price_AUD' => '325',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
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	'online' => true,
	'master' => false,
	'last_datasheet_update' => 'January 30, 2018',
	'slug' => 'c2c2-polyclonal-antibody-20-ul',
	'meta_title' => 'C2C2 Polyclonal Antibody | Diagenode',
	'meta_keywords' => '',
	'meta_description' => 'C2C2 Polyclonal Antibody validated  in WB. Batch-specific data available on the website. Sample size available.',
	'modified' => '2024-01-16 13:22:07',
	'created' => '2018-03-05 11:50:10',
	'ProductsGroup' => array(
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$old_catalog_number = ''
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	'info1' => '',
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	'label3' => '',
	'info3' => '',
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	'last_datasheet_update' => 'January 30, 2018',
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	'meta_description' => 'C2C2 Polyclonal Antibody validated  in WB. Batch-specific data available on the website. Sample size available.',
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$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
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	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'online' => true,
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	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'ProductsApplication' => array(
		'id' => '4869',
		'product_id' => '2963',
		'application_id' => '19'
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$applications = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
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	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
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	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'locale' => 'eng'
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$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
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	'name' => 'C2C2 polyclonal antibody',
	'description' => '<p>Polyclonal antibody raised in rabbit against the class 2 type VI CRISPR-Cas effector (C2C2) Leptotrichia shahii using a recombinant protein.</p>',
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	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/c2c2-polyclonal-antibody-100-ul.pdf',
	'slug' => 'c2c2-polyclonal-antibody-tds',
	'meta_keywords' => '',
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	'modified' => '2018-03-05 11:58:46',
	'created' => '2018-03-05 11:58:46',
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	'url' => 'files/SDS/C2C2/SDS-C15310267-C2C2_Antibody-ES-es-GHS_2_0.pdf',
	'countries' => 'ES',
	'modified' => '2024-01-16 13:17:58',
	'created' => '2024-01-16 13:17:58',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
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	'meta_keywords' => '',
	'meta_description' => 'C2C2 Polyclonal Antibody validated  in WB. Batch-specific data available on the website. Sample size available. ',
	'meta_title' => 'C2C2 Polyclonal Antibody | Diagenode',
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			'antibody_id' => '522',
			'name' => 'C2C2 Antibody',
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<div class="small-4 columns">
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</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against C2C2</strong><br /> Western blot was performed on protein extracts from U2OS cells (lane 1) and on extracts from U2OS cells spiked with 50 ng of the immunogen (Lsh C2C2 recombinant protein, lane 2) using the Diagenode antibody against C2C2 (Cat. No. C15310267), diluted 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the C2C2 protein is indicated on the right. It&rsquo;s unclear what the higher MW signal represents but since it is absent in the lane without the spiked protein, it might be a protein aggregate.</small></p>
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			'info2' => '',
			'label3' => '',
			'info3' => '',
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			'meta_title' => 'C2C2 Polyclonal Antibody | Diagenode',
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			'concentration' => 'Not determined',
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<th>Suggested dilution</th>
<th>References</th>
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<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1/10,000</td>
<td>Fig 1</td>
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</tbody>
</table>',
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			'uniprot_acc' => '',
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			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2018-03-15 16:14:50',
			'created' => '2018-03-05 11:20:26',
			'select_label' => '522 - C2C2 polyclonal antibody (A2915-004 - Not determined - <em>Leptotrichia shahii</em> - Whole antiserum from rabbit containing 0.05% azide. - Rabbit)'
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$meta_description = 'C2C2 Polyclonal Antibody validated  in WB. Batch-specific data available on the website. Sample size available. '
$meta_title = 'C2C2 Polyclonal Antibody | Diagenode'
$product = array(
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		'antibody_id' => '522',
		'name' => 'C2C2 Antibody',
		'description' => '<p>Polyclonal antibody raised in rabbit against the class 2 type VI CRISPR-Cas effector (C2C2) <em>Leptotrichia shahii</em> using a recombinant protein.</p>',
		'label1' => 'Validation data',
		'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310267-wb.jpg" alt="Western blot figure 1" style="display: block; margin-left: auto; margin-right: auto;" width="272" height="350" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against C2C2</strong><br /> Western blot was performed on protein extracts from U2OS cells (lane 1) and on extracts from U2OS cells spiked with 50 ng of the immunogen (Lsh C2C2 recombinant protein, lane 2) using the Diagenode antibody against C2C2 (Cat. No. C15310267), diluted 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the C2C2 protein is indicated on the right. It&rsquo;s unclear what the higher MW signal represents but since it is absent in the lane without the spiked protein, it might be a protein aggregate.</small></p>
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		'price_AUD' => '1450',
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		'last_datasheet_update' => 'January 30, 2018',
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		'meta_title' => 'C2C2 Polyclonal Antibody | Diagenode',
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		'host' => '*****',
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		'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified in <em>Leptotrichia shahii</em>. This CRISPR system is characterized by the presence of the single effector protein C2C2. C2C2 lacks homology to any known DNA nuclease domain but contains two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and is thought to function solely as an RNA-guided RNA-targeting CRISPR effector.',
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		'isotype' => '',
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		'concentration' => 'Not determined',
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		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>Western Blotting</td>
<td>1:1,000 - 1/10,000</td>
<td>Fig 1</td>
</tr>
</tbody>
</table>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => 'c2c2-polyclonal-antibody',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2018-03-15 16:14:50',
		'created' => '2018-03-05 11:20:26',
		'select_label' => '522 - C2C2 polyclonal antibody (A2915-004 - Not determined - <em>Leptotrichia shahii</em> - Whole antiserum from rabbit containing 0.05% azide. - Rabbit)'
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode antibody directed against C2C2</strong><br /> Western blot was performed on protein extracts from U2OS cells (lane 1) and on extracts from U2OS cells spiked with 50 ng of the immunogen (Lsh C2C2 recombinant protein, lane 2) using the Diagenode antibody against C2C2 (Cat. No. C15310267), diluted 1:10,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the C2C2 protein is indicated on the right. It&rsquo;s unclear what the higher MW signal represents but since it is absent in the lane without the spiked protein, it might be a protein aggregate.</small></p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<td>Fig 1</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
C2C2 polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against the class 2 type VI CRISPR-Cas effector (C2C2) Lepto...	Download

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