Alternative names: SGF29
Polyclonal antibody raised in rabbit against human CCDC101 (coiled-coil domain containing 101), using two KLH-conjugated synthetic peptides containing a sequence from the central and from the C-terminal part of the protein, respectively.
| Lot | A509-004 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 – 1:200 | Fig 1 |
| Western Blotting | 1:1,000 | Fig 2 |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human CCDC101 (Cat. No. CS-122-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,700.
Figure 2. Western blot analysis using the Diagenode antibody directed against CCDC101
Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against CCDC101 (Cat. No. CS-122-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 33 kDa) is indicated on the right; the marker (in kDa) is shown on the left.
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