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Polyclonal antibody raised in rabbit against human CHAMP1 (chromosome alignment maintaining phosphoprotein 1) using two KLH-conjugated synthetic peptides containing a sequence from the central region of the protein.
Lot
A1018-001
Concentration
not determined
Species reactivity
Human
Type
Polyclonal
Purity
Whole antiserum
Host
Rabbit
Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications
Suggested dilution
References
ELISA
1:500
Fig 1
* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:1,000 dilution
Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human CHAMP1 (cat. No. CS- PA050-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:8,500.
