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Polyclonal antibody raised in rabbit against human CHD1 (Chromodomain Helicase DNA Binding Protein 1), using a synthetic peptide containing a sequence from the C-terminal part of the protein.
| Lot | A301-218A1 |
|---|---|
| Concentration | 0.2 μg/μl |
| Species reactivity | Human |
| Type | Polyclonal ChIP grade, ChIP-seq grade |
| Purity | Affinity purified |
| Host | Rabbit |
| Storage Conditions | Store at 4°C. |
| Storage Buffer | TBS containing 0.1% BSA and 0.09% sodium azide. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP/ChIP-seq* | 1 μg/ChIP | Fig 1, 2 |
| Western Blotting | 1:2,000 | Fig 3 |
| IP | 3 μg per IP | Fig 4 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.
1Manufactured by Bethyl Laboratories, Inc., Texas, USA
Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1
ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1
ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).
Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1
Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1
Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200.
CHD1 (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.
| ChIP-qPCR (ab) Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| IP Immunoprecipitation Read more |
| ChIP-seq (ab) Read more |
| CHD1 polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against human CHD1 (Chromodomain Helicase DNA Binding Protei... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: CHD1 Antibody (Diagenode Cat# C15410334 Lot# A301-218A1). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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', 'meta_title' => 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2868', 'antibody_id' => '494', 'name' => 'CHD1 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CHD1 (Chromodomain Helicase DNA Binding Protein 1)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-chip.png" alt="CHD1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p><strong>CHD1</strong> (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410334', 'old_catalog_number' => '', 'sf_code' => 'C15410334-D001-001161', 'type' => 'REF', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 13, 2017', 'slug' => 'chd1-polyclonal-antibody-100-ul', 'meta_title' => 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode', 'meta_keywords' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) polyclonal antibody,Immunoprecipitation,Western blot', 'meta_description' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. 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Batch-specific data available on the website. ' $meta_title = 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode' $product = array( 'Product' => array( 'id' => '2868', 'antibody_id' => '494', 'name' => 'CHD1 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CHD1 (Chromodomain Helicase DNA Binding Protein 1)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-chip.png" alt="CHD1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p><strong>CHD1</strong> (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. 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Batch-specific data available on the website. ', 'modified' => '2022-02-14 13:30:26', 'created' => '2016-12-12 16:00:58', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '494', 'name' => 'CHD1 polyclonal antibody', 'description' => 'CHD1 (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.', 'clonality' => '', 'isotype' => '', 'lot' => 'A301-218A1', 'concentration' => '0.2 μg/μl', 'reactivity' => 'Human', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Affinity purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq<sup>*</sup></td> <td>1 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td><span>Western Blotting</span></td> <td>1:2,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td><span>3 μg per IP</span></td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => 'TBS containing 0.1% BSA and 0.09% sodium azide.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'chd1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-01-07 11:10:58', 'created' => '2016-12-12 16:21:52', 'select_label' => '494 - CHD1 polyclonal antibody (A301-218A1 - 0.2 μg/μl - Human - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '336', 'name' => 'C15410334', 'product_id' => '2868', 'modified' => '2020-12-30 12:04:27', 'created' => '2020-12-30 12:04:27' ) ), 'Group' => array( 'Group' => array( 'id' => '336', 'name' => 'C15410334', 'product_id' => '2868', 'modified' => '2020-12-30 12:04:27', 'created' => '2020-12-30 12:04:27' ), 'Master' => array( 'id' => '2868', 'antibody_id' => '494', 'name' => 'CHD1 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CHD1 (Chromodomain Helicase DNA Binding Protein 1)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-chip.png" alt="CHD1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). 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Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '18', 'position' => '20', 'parent_id' => '4', 'name' => 'Chromatin associated proteins', 'description' => '<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) polyclonal antibody,Immunoprecipitation,Western blot', 'meta_description' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website. ', 'meta_title' => 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2868', 'antibody_id' => '494', 'name' => 'CHD1 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CHD1 (Chromodomain Helicase DNA Binding Protein 1)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-chip.png" alt="CHD1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). 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CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410334', 'old_catalog_number' => '', 'sf_code' => 'C15410334-D001-001161', 'type' => 'REF', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 13, 2017', 'slug' => 'chd1-polyclonal-antibody-100-ul', 'meta_title' => 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode', 'meta_keywords' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) polyclonal antibody,Immunoprecipitation,Western blot', 'meta_description' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. 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CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.', 'clonality' => '', 'isotype' => '', 'lot' => 'A301-218A1', 'concentration' => '0.2 μg/μl', 'reactivity' => 'Human', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Affinity purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq<sup>*</sup></td> <td>1 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td><span>Western Blotting</span></td> <td>1:2,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td><span>3 μg per IP</span></td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. 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Batch-specific data available on the website. ' $meta_title = 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode' $product = array( 'Product' => array( 'id' => '2868', 'antibody_id' => '494', 'name' => 'CHD1 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CHD1 (Chromodomain Helicase DNA Binding Protein 1)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-chip.png" alt="CHD1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p><strong>CHD1</strong> (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'chd1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-01-07 11:10:58', 'created' => '2016-12-12 16:21:52', 'select_label' => '494 - CHD1 polyclonal antibody (A301-218A1 - 0.2 μg/μl - Human - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '336', 'name' => 'C15410334', 'product_id' => '2868', 'modified' => '2020-12-30 12:04:27', 'created' => '2020-12-30 12:04:27' ) ), 'Group' => array( 'Group' => array( 'id' => '336', 'name' => 'C15410334', 'product_id' => '2868', 'modified' => '2020-12-30 12:04:27', 'created' => '2020-12-30 12:04:27' ), 'Master' => array( 'id' => '2868', 'antibody_id' => '494', 'name' => 'CHD1 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CHD1 (Chromodomain Helicase DNA Binding Protein 1)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-chip.png" alt="CHD1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p><strong>CHD1</strong> (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410334', 'old_catalog_number' => '', 'sf_code' => 'C15410334-D001-001161', 'type' => 'REF', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 13, 2017', 'slug' => 'chd1-polyclonal-antibody-100-ul', 'meta_title' => 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode', 'meta_keywords' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) polyclonal antibody,Immunoprecipitation,Western blot', 'meta_description' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. 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', 'modified' => '2022-02-14 13:30:26', 'created' => '2016-12-12 16:00:58' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. 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Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '18', 'position' => '20', 'parent_id' => '4', 'name' => 'Chromatin associated proteins', 'description' => '<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) polyclonal antibody,Immunoprecipitation,Western blot', 'meta_description' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website. ', 'meta_title' => 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2868', 'antibody_id' => '494', 'name' => 'CHD1 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CHD1 (Chromodomain Helicase DNA Binding Protein 1)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-chip.png" alt="CHD1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p><strong>CHD1</strong> (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410334', 'old_catalog_number' => '', 'sf_code' => 'C15410334-D001-001161', 'type' => 'REF', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 13, 2017', 'slug' => 'chd1-polyclonal-antibody-100-ul', 'meta_title' => 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode', 'meta_keywords' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) polyclonal antibody,Immunoprecipitation,Western blot', 'meta_description' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. 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CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.', 'clonality' => '', 'isotype' => '', 'lot' => 'A301-218A1', 'concentration' => '0.2 μg/μl', 'reactivity' => 'Human', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Affinity purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq<sup>*</sup></td> <td>1 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td><span>Western Blotting</span></td> <td>1:2,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td><span>3 μg per IP</span></td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => 'TBS containing 0.1% BSA and 0.09% sodium azide.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'chd1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-01-07 11:10:58', 'created' => '2016-12-12 16:21:52', 'select_label' => '494 - CHD1 polyclonal antibody (A301-218A1 - 0.2 μg/μl - Human - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/chd1-polyclonal-antibody-100-ul' ) $language = 'en' $meta_keywords = 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) polyclonal antibody,Immunoprecipitation,Western blot' $meta_description = 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website. ' $meta_title = 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode' $product = array( 'Product' => array( 'id' => '2868', 'antibody_id' => '494', 'name' => 'CHD1 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CHD1 (Chromodomain Helicase DNA Binding Protein 1)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-chip.png" alt="CHD1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p><strong>CHD1</strong> (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410334', 'old_catalog_number' => '', 'sf_code' => 'C15410334-D001-001161', 'type' => 'REF', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 13, 2017', 'slug' => 'chd1-polyclonal-antibody-100-ul', 'meta_title' => 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode', 'meta_keywords' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) polyclonal antibody,Immunoprecipitation,Western blot', 'meta_description' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website. ', 'modified' => '2022-02-14 13:30:26', 'created' => '2016-12-12 16:00:58', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '494', 'name' => 'CHD1 polyclonal antibody', 'description' => 'CHD1 (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.', 'clonality' => '', 'isotype' => '', 'lot' => 'A301-218A1', 'concentration' => '0.2 μg/μl', 'reactivity' => 'Human', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Affinity purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq<sup>*</sup></td> <td>1 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td><span>Western Blotting</span></td> <td>1:2,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td><span>3 μg per IP</span></td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => 'TBS containing 0.1% BSA and 0.09% sodium azide.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'chd1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-01-07 11:10:58', 'created' => '2016-12-12 16:21:52', 'select_label' => '494 - CHD1 polyclonal antibody (A301-218A1 - 0.2 μg/μl - Human - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '336', 'name' => 'C15410334', 'product_id' => '2868', 'modified' => '2020-12-30 12:04:27', 'created' => '2020-12-30 12:04:27' ) ), 'Group' => array( 'Group' => array( 'id' => '336', 'name' => 'C15410334', 'product_id' => '2868', 'modified' => '2020-12-30 12:04:27', 'created' => '2020-12-30 12:04:27' ), 'Master' => array( 'id' => '2868', 'antibody_id' => '494', 'name' => 'CHD1 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CHD1 (Chromodomain Helicase DNA Binding Protein 1)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-chip.png" alt="CHD1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p><strong>CHD1</strong> (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410334', 'old_catalog_number' => '', 'sf_code' => 'C15410334-D001-001161', 'type' => 'REF', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 13, 2017', 'slug' => 'chd1-polyclonal-antibody-100-ul', 'meta_title' => 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode', 'meta_keywords' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) polyclonal antibody,Immunoprecipitation,Western blot', 'meta_description' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website. ', 'modified' => '2022-02-14 13:30:26', 'created' => '2016-12-12 16:00:58' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '18', 'position' => '20', 'parent_id' => '4', 'name' => 'Chromatin associated proteins', 'description' => '<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) polyclonal antibody,Immunoprecipitation,Western blot', 'meta_description' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website. ', 'meta_title' => 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode', 'product' => array( 'Product' => array( 'id' => '2868', 'antibody_id' => '494', 'name' => 'CHD1 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CHD1 (Chromodomain Helicase DNA Binding Protein 1)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-chip.png" alt="CHD1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). 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CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.', 'clonality' => '', 'isotype' => '', 'lot' => 'A301-218A1', 'concentration' => '0.2 μg/μl', 'reactivity' => 'Human', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Affinity purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq<sup>*</sup></td> <td>1 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td><span>Western Blotting</span></td> <td>1:2,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td><span>3 μg per IP</span></td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => 'TBS containing 0.1% BSA and 0.09% sodium azide.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'chd1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-01-07 11:10:58', 'created' => '2016-12-12 16:21:52', 'select_label' => '494 - CHD1 polyclonal antibody (A301-218A1 - 0.2 μg/μl - Human - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/chd1-polyclonal-antibody-100-ul' ) $language = 'en' $meta_keywords = 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) polyclonal antibody,Immunoprecipitation,Western blot' $meta_description = 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website. ' $meta_title = 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode' $product = array( 'Product' => array( 'id' => '2868', 'antibody_id' => '494', 'name' => 'CHD1 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CHD1 (Chromodomain Helicase DNA Binding Protein 1)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-chip.png" alt="CHD1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p><strong>CHD1</strong> (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410334', 'old_catalog_number' => '', 'sf_code' => 'C15410334-D001-001161', 'type' => 'REF', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 13, 2017', 'slug' => 'chd1-polyclonal-antibody-100-ul', 'meta_title' => 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode', 'meta_keywords' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) polyclonal antibody,Immunoprecipitation,Western blot', 'meta_description' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. 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CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.', 'clonality' => '', 'isotype' => '', 'lot' => 'A301-218A1', 'concentration' => '0.2 μg/μl', 'reactivity' => 'Human', 'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>', 'purity' => 'Affinity purified', 'classification' => 'Classic', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq<sup>*</sup></td> <td>1 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td><span>Western Blotting</span></td> <td>1:2,000</td> <td>Fig 3</td> </tr> <tr> <td>IP</td> <td><span>3 μg per IP</span></td> <td>Fig 4</td> </tr> </tbody> </table> <p></p> <p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p> <p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>', 'storage_conditions' => 'Store at 4°C.', 'storage_buffer' => 'TBS containing 0.1% BSA and 0.09% sodium azide.', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => 'chd1-polyclonal-antibody', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-01-07 11:10:58', 'created' => '2016-12-12 16:21:52', 'select_label' => '494 - CHD1 polyclonal antibody (A301-218A1 - 0.2 μg/μl - Human - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '336', 'name' => 'C15410334', 'product_id' => '2868', 'modified' => '2020-12-30 12:04:27', 'created' => '2020-12-30 12:04:27' ) ), 'Group' => array( 'Group' => array( 'id' => '336', 'name' => 'C15410334', 'product_id' => '2868', 'modified' => '2020-12-30 12:04:27', 'created' => '2020-12-30 12:04:27' ), 'Master' => array( 'id' => '2868', 'antibody_id' => '494', 'name' => 'CHD1 Antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CHD1 (Chromodomain Helicase DNA Binding Protein 1)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-chip.png" alt="CHD1 Antibody ChIP Grade" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CHD1</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CHD1 (Cat. No. C15410334) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active ACTB and EIF4A2 genes, used as positive control, and for the inactive MYT1 gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-a.jpg" alt="CHD1 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-b.jpg" alt="CHD1 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-c.jpg" alt="CHD1 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410334-chipseq-d.jpg" alt="CHD1 Antibody validated in ChIP-seq" /></div> </div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="extra-spaced"></div> <div class="row"> <div class="small-12 columns"> <p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CHD1</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2.5 Mb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the ACTB and EIF4A2 positive control genes (fig 2C and D).</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-wb.png" alt="CHD1 Antibody validated in Western Blot" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CHD1</strong><br /> Whole cell extracts from 293T cells were analysed by Western blot using the Diagenode antibody against CHD1 (Cat. No. C15410334) diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15410334-ip.png" alt="CHD1 Antibody validated in Immunoprecipitation" width="100" /></p> </div> <div class="small-9 columns"> <p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CHD1</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against CHD1 (Cat. No. C15410334, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CHD1 protein was detected by western blot with the CHD1 antibody diluted 1:200. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p><strong>CHD1</strong> (UniProtKB/Swiss-Prot entry O14646) functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. It is required for the recognition of H3K4me3 by the FACT and PAF complexes as well as the U2 snRNP complex and regulates both polymerase II and polymerase I transcription. CHD1 further negatively regulates DNA replication and is required for maintaining open chromatin and pluripotency in embryonic stem cells.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410334', 'old_catalog_number' => '', 'sf_code' => 'C15410334-D001-001161', 'type' => 'REF', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '460', 'price_GBP' => '380', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1150', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'January 13, 2017', 'slug' => 'chd1-polyclonal-antibody-100-ul', 'meta_title' => 'CHD1 Antibody - ChIP-seq Grade (C15410334) | Diagenode', 'meta_keywords' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) polyclonal antibody,Immunoprecipitation,Western blot', 'meta_description' => 'CHD1 (Chromodomain Helicase DNA Binding Protein 1) Polyclonal Antibody validated in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website. ', 'modified' => '2022-02-14 13:30:26', 'created' => '2016-12-12 16:00:58' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '18', 'position' => '20', 'parent_id' => '4', 'name' => 'Chromatin associated proteins', 'description' => '<p><span style="font-weight: 400;">Diagenode’s large range of highly validated antibodies includes the antibodies to study chromatin-associated proteins. These antibodies have been thoroughly validated for many applications (ChIP, ChIP-seq, WB, IF, ELISA, Dot blot, IHC, IP). Check out all of our highly sensitive and specific antibodies for chromatin-associated proteins studies below.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chromatin-modifying-and-or-associated-proteins', 'cookies_tag_id' => null, 'meta_keywords' => 'Chromatin modifying and or associated proteins,polyclonal antibody', 'meta_description' => 'Diagenode Offers a Wide Range of Chromatin Modifying and all other Associated Proteins and Check out all of our Highly Sensitive and Specific Antibodies for Chromatin-Associated Proteins', 'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode', 'modified' => '2019-07-04 16:19:01', 'created' => '2014-09-13 08:16:51', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation 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'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '3173', 'antibody_id' => '634', 'name' => 'CHD1 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410334-20', 'old_catalog_number' => '', 'sf_code' => 'C15410334-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'chd1-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 12:19:02', 'created' => '2020-12-30 12:04:04', 'ProductsGroup' => array( 'id' => '377', 'product_id' => '3173', 'group_id' => '336' ) ) ) $pro = array( 'id' => '3173', 'antibody_id' => '634', 'name' => 'CHD1 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410334-20', 'old_catalog_number' => '', 'sf_code' => 'C15410334-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'chd1-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 12:19:02', 'created' => '2020-12-30 12:04:04', 'ProductsGroup' => array( 'id' => '377', 'product_id' => '3173', 'group_id' => '336' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $other_format = array( 'id' => '3173', 'antibody_id' => '634', 'name' => 'CHD1 Antibody (sample size)', 'description' => '', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15410334-20', 'old_catalog_number' => '', 'sf_code' => 'C15410334-361', 'type' => 'REF', 'search_order' => '03-Antibody', 'price_EUR' => '110', 'price_USD' => '120', 'price_GBP' => '105', 'price_JPY' => '17230', 'price_CNY' => '', 'price_AUD' => '300', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'chd1-polyclonal-antibody-20', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-12-23 12:19:02', 'created' => '2020-12-30 12:04:04', 'ProductsGroup' => array( 'id' => '377', 'product_id' => '3173', 'group_id' => '336' ) ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( 'id' => '4590', 'product_id' => '2868', 'application_id' => '42' ) ) $slugs = array( (int) 0 => 'chip-seq-antibodies' ) $applications = array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'locale' => 'eng' ) $description = '' $name = 'ChIP-seq (ab)' $document = array( 'id' => '934', 'name' => 'CHD1 polyclonal antibody', 'description' => '<p>Polyclonal antibody raised in rabbit against human CHD1 (Chromodomain Helicase DNA Binding Protein 1), using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_chd1_C15410334.pdf', 'slug' => 'datasheet-chd1-c15410334', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-12-13 11:57:39', 'created' => '2016-12-13 11:57:39', 'ProductsDocument' => array( 'id' => '2269', 'product_id' => '2868', 'document_id' => '934' ) ) $sds = array( 'id' => '996', 'name' => 'CHD1 antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/CHD1/SDS-C15410334-CHD1_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2021-01-26 10:23:19', 'created' => '2021-01-26 10:23:19', 'ProductsSafetySheet' => array( 'id' => '1742', 'product_id' => '2868', 'safety_sheet_id' => '996' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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