| DiaMag anti-mouse IgG-coated magnetic beads DATASHEET These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chrom... | Download |
These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.
| DiaMag anti-mouse IgG-coated magnetic beads DATASHEET These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chrom... | Download |
| DiaMag anti-mouse IgG coated magnetic beads SDS GB en | Download |
| DiaMag anti-mouse IgG coated magnetic beads SDS US en | Download |
| DiaMag anti-mouse IgG coated magnetic beads SDS DE de | Download |
| DiaMag anti-mouse IgG coated magnetic beads SDS JP ja | Download |
| DiaMag anti-mouse IgG coated magnetic beads SDS BE nl | Download |
| DiaMag anti-mouse IgG coated magnetic beads SDS BE fr | Download |
| DiaMag anti-mouse IgG coated magnetic beads SDS FR fr | Download |
| DiaMag anti-mouse IgG coated magnetic beads SDS ES es | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: DiaMag anti-mouse IgG coated magnetic beads (Diagenode Cat# C03010022-220). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Identification of a PadR-type regulator essential for intracellularpathogenesis of |
Formation of the CenH3-Deficient Holocentromere in Lepidoptera AvoidsActive Chromatin. |
Novel dual regulators of Pseudomonas aeruginosa essential for productive biofilms and virulence. |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'product' => array( 'Product' => array( 'id' => '1911', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '220 µl', 'catalog_number' => 'C03010022-220', 'old_catalog_number' => 'kch-821-220', 'sf_code' => 'C03010022-D001-000572', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '100', 'price_USD' => '125', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '312', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-220-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:27', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/diamag-anti-mouse-igg-coated-magnetic-beads-220-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'DiaMag anti-mouse IgG coated magnetic beads' $meta_title = 'DiaMag anti-mouse IgG coated magnetic beads' $product = array( 'Product' => array( 'id' => '1911', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '220 µl', 'catalog_number' => 'C03010022-220', 'old_catalog_number' => 'kch-821-220', 'sf_code' => 'C03010022-D001-000572', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '100', 'price_USD' => '125', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '312', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-220-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:27', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '167', 'name' => 'C03010022', 'product_id' => '1911', 'modified' => '2016-02-23 20:52:11', 'created' => '2016-02-23 20:52:11' ) ), 'Group' => array( 'Group' => array( 'id' => '167', 'name' => 'C03010022', 'product_id' => '1911', 'modified' => '2016-02-23 20:52:11', 'created' => '2016-02-23 20:52:11' ), 'Master' => array( 'id' => '1911', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '220 µl', 'catalog_number' => 'C03010022-220', 'old_catalog_number' => 'kch-821-220', 'sf_code' => 'C03010022-D001-000572', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '100', 'price_USD' => '125', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '312', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-220-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:27', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '1885', 'antibody_id' => null, 'name' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'description' => '<p><span>The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation analysis. It features</span><span> a highly specific monoclonal antibody against </span><span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA</span><span>. It includes control DNA and primers to assess the effiency of the assay. </span><span>Performing hydroxymethylation profiling with the hMeDIP kit is fast, reliable and highly specific.</span></p>', 'label1' => ' Characteristics', 'info1' => '<ul style="list-style-type: disc;"> <li><span>Robust enrichment & immunoprecipitation of hydroxymethylated DNA</span></li> <li>Highly specific monoclonal antibody against 5-hmC<span> for reliable, reproducible results</span></li> <li>Including control DNA and primers to <span>monitor the efficiency of the assay</span> <ul style="list-style-type: circle;"> <li>5-hmC, 5-mC and unmethylated DNA sequences and primer pairs</li> <li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li> </ul> </li> </ul> <ul style="list-style-type: disc;"> <li>Improved single-tube, magnetic bead-based protocol</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '16 rxns', 'catalog_number' => 'C02010034', 'old_catalog_number' => '', 'sf_code' => 'C02010034-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '630', 'price_USD' => '690', 'price_GBP' => '580', 'price_JPY' => '98690', 'price_CNY' => '', 'price_AUD' => '1725', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns', 'meta_title' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'meta_keywords' => '', 'meta_description' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'modified' => '2021-01-18 10:37:19', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '46', 'position' => '1', 'parent_id' => '34', 'name' => 'Magnetic beads', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'magnetic-beads', 'cookies_tag_id' => null, 'meta_keywords' => 'Magnetic beads,chromatin immunoprecipitation assay,DNA immunoprecipitation ', 'meta_description' => 'Diagenode's Magnetic beads are extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP)', 'meta_title' => 'Magnetic beads for ChIP assay | DIagenode', 'modified' => '2016-02-24 15:05:50', 'created' => '2015-07-07 16:53:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '911', 'name' => 'DiaMag anti-mouse IgG-coated magnetic beads', 'description' => '<p>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP).</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/c03010022-kch-821.pdf', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-19 10:45:17', 'created' => '2016-07-19 10:45:17', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4320', 'name' => 'Identification of a PadR-type regulator essential for intracellularpathogenesis of', 'authors' => 'McMillan Ian A. et al. ', 'description' => '<p>Burkholderia pseudomallei (Bp) is the causative agent of melioidosis, a disease endemic to the tropics. Melioidosis manifests in various ways ranging from acute skin lesions to pneumonia and, in rare cases, infection of the central nervous system. Bp is a facultative intracellular pathogen and it can infect various cell types. The Bp intracellular lifecycle has been partially elucidated and is highly complex. Herein, we have identified a transcriptional regulator, BP1026B_II1198, that is differentially expressed as Bp transits through host cells. A deletion mutant of BP1026B_II1198 was attenuated in RAW264.7 cell and BALB/c mouse infection. To further characterize the function of this transcriptional regulator, we endeavored to determine the regulon of BP1026B_II1198. RNA-seq analysis showed the global picture of genes regulated while ChIP-seq analysis identified two specific BP1026B_II1198 binding regions on chromosome II. We investigated the transposon mutants of these genes controlled by BP1026B_II1198 and confirmed that these genes contribute to pathogenesis in RAW264.7 murine macrophage cells. Taken together, the data presented here shed light on the regulon of BP1026B_II1198 and its role during intracellular infection and highlights an integral portion of the highly complex regulation network of Bp during host infection.</p>', 'date' => '2021-05-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34001967', 'doi' => '10.1038/s41598-021-89852-7', 'modified' => '2022-08-02 16:55:52', 'created' => '2022-05-19 10:41:50', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '4092', 'name' => 'Formation of the CenH3-Deficient Holocentromere in Lepidoptera AvoidsActive Chromatin.', 'authors' => 'Senaratne, Aruni P and Muller, Héloïse and Fryer, Kelsey A and Kawamoto,Munetaka and Katsuma, Susumu and Drinnenberg, Ines A', 'description' => '<p>Despite the essentiality for faithful chromosome segregation, centromere architectures are diverse among eukaryotes and embody two main configurations: mono- and holocentromeres, referring, respectively, to localized or unrestricted distribution of centromeric activity. Of the two, some holocentromeres offer the curious condition of having arisen independently in multiple insects, most of which have lost the otherwise essential centromere-specifying factor CenH3 (first described as CENP-A in humans). The loss of CenH3 raises intuitive questions about how holocentromeres are organized and regulated in CenH3-lacking insects. Here, we report the first chromatin-level description of CenH3-deficient holocentromeres by leveraging recently identified centromere components and genomics approaches to map and characterize the holocentromeres of the silk moth Bombyx mori, a representative lepidopteran insect lacking CenH3. This uncovered a robust correlation between the distribution of centromere sites and regions of low chromatin activity along B. mori chromosomes. Transcriptional perturbation experiments recapitulated the exclusion of B. mori centromeres from active chromatin. Based on reciprocal centromere occupancy patterns observed along differentially expressed orthologous genes of Lepidoptera, we further found that holocentromere formation in a manner that is recessive to chromatin dynamics is evolutionarily conserved. Our results help us discuss the plasticity of centromeres in the context of a role for the chromosome-wide chromatin landscape in conferring centromere identity rather than the presence of CenH3. Given the co-occurrence of CenH3 loss and holocentricity in insects, we further propose that the evolutionary establishment of holocentromeres in insects was facilitated through the loss of a CenH3-specified centromere.</p>', 'date' => '2020-10-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33125865', 'doi' => '10.1016/j.cub.2020.09.078', 'modified' => '2021-03-17 17:13:50', 'created' => '2021-02-18 10:21:53', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3598', 'name' => 'Novel dual regulators of Pseudomonas aeruginosa essential for productive biofilms and virulence.', 'authors' => 'Heacock-Kang Y, Zarzycki-Siek J, Sun Z, Poonsuk K, Bluhm AP, Cabanas D, Fogen D, McMillan IA, Chuanchuen R, Hoang TT', 'description' => '<p>Gene regulation network in Pseudomonas aeruginosa is complex. With a relatively large genome (6.2 Mb), there is a significant portion of genes that are proven or predicted to be transcriptional regulators. Many of these regulators have been shown to play important roles in biofilm formation and maintenance. In this study, we present a novel transcriptional regulator, PA1226, which modulates biofilm formation and virulence in P. aeruginosa. Mutation in the gene encoding this regulator abolished the ability of P. aeruginosa to produce biofilms in vitro, without any effect on the planktonic growth. This regulator is also essential for the in vivo fitness and pathogenesis in both Drosophila melanogaster and BALB/c mouse lung infection models. Transcriptome analysis revealed that PA1226 regulates many essential virulence genes/pathways, including those involved in alginate, pili, and LPS biosynthesis. Genes/operons directly regulated by PA1226 and potential binding sequences were identified via ChIP-seq. Attempts to confirm the binding sequences by electrophoretic mobility shift assay led to the discovery of a co-regulator, PA1413, via co-immunoprecipitation assay. PA1226 and PA1413 were shown to bind collaboratively to the promoter regions of their regulons. A model is proposed, summarizing our finding on this novel dual-regulation system.</p>', 'date' => '2018-08-01', 'pmid' => 'http://www.pubmed.gov/29995308', 'doi' => '10.1111/mmi.14063', 'modified' => '2019-04-17 15:07:51', 'created' => '2019-04-16 12:25:30', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '334', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS GB en', 'language' => 'en', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-GB-en-GHS_1_0.pdf', 'countries' => 'GB', 'modified' => '2020-06-09 13:01:35', 'created' => '2020-06-09 13:01:35', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '336', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS US en', 'language' => 'en', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-US-en-GHS_1_0.pdf', 'countries' => 'US', 'modified' => '2020-06-09 13:03:27', 'created' => '2020-06-09 13:03:27', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '331', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS DE de', 'language' => 'de', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-DE-de-GHS_1_0.pdf', 'countries' => 'DE', 'modified' => '2020-06-09 12:59:50', 'created' => '2020-06-09 12:59:50', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '335', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-06-09 13:02:44', 'created' => '2020-06-09 13:02:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '330', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-BE-nl-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 12:59:19', 'created' => '2020-06-09 12:59:19', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '329', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-BE-fr-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 12:58:49', 'created' => '2020-06-09 12:58:49', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '333', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-FR-fr-GHS_1_0.pdf', 'countries' => 'FR', 'modified' => '2020-06-09 13:00:53', 'created' => '2020-06-09 13:00:53', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '332', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS ES es', 'language' => 'es', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 13:00:23', 'created' => '2020-06-09 13:00:23', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/diamag-anti-mouse-igg-coated-magnetic-beads-220-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '1910', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1.5 ml', 'catalog_number' => 'C03010022-150', 'old_catalog_number' => 'kch-821-150', 'sf_code' => 'C03010022-351', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '575', 'price_USD' => '405', 'price_GBP' => '520', 'price_JPY' => '90075', 'price_CNY' => '', 'price_AUD' => '1012', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-1-5-ml', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2016-07-19 10:45:27', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '178', 'product_id' => '1910', 'group_id' => '167' ) ), (int) 1 => array( 'id' => '1912', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '660 µl', 'catalog_number' => 'C03010022-660', 'old_catalog_number' => 'kch-821-660', 'sf_code' => 'C03010022-350', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '265', 'price_USD' => '250', 'price_GBP' => '245', 'price_JPY' => '41510', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-660-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:02', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '179', 'product_id' => '1912', 'group_id' => '167' ) ) ) $pro = array( 'id' => '1912', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '660 µl', 'catalog_number' => 'C03010022-660', 'old_catalog_number' => 'kch-821-660', 'sf_code' => 'C03010022-350', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '265', 'price_USD' => '250', 'price_GBP' => '245', 'price_JPY' => '41510', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-660-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:02', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '179', 'product_id' => '1912', 'group_id' => '167' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C02010034</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1885" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/1885" id="CartAdd/1885Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1885" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto hMeDIP kit x16 (monoclonal mouse antibody)</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'C02010034', '690', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'C02010034', '690', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns" data-reveal-id="cartModal-1885" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Auto hMeDIP kit x16 (monoclonal mouse antibody)</h6> </div> </div> </li> ' $related = array( 'id' => '1885', 'antibody_id' => null, 'name' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'description' => '<p><span>The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation analysis. It features</span><span> a highly specific monoclonal antibody against </span><span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA</span><span>. It includes control DNA and primers to assess the effiency of the assay. </span><span>Performing hydroxymethylation profiling with the hMeDIP kit is fast, reliable and highly specific.</span></p>', 'label1' => ' Characteristics', 'info1' => '<ul style="list-style-type: disc;"> <li><span>Robust enrichment & immunoprecipitation of hydroxymethylated DNA</span></li> <li>Highly specific monoclonal antibody against 5-hmC<span> for reliable, reproducible results</span></li> <li>Including control DNA and primers to <span>monitor the efficiency of the assay</span> <ul style="list-style-type: circle;"> <li>5-hmC, 5-mC and unmethylated DNA sequences and primer pairs</li> <li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li> </ul> </li> </ul> <ul style="list-style-type: disc;"> <li>Improved single-tube, magnetic bead-based protocol</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '16 rxns', 'catalog_number' => 'C02010034', 'old_catalog_number' => '', 'sf_code' => 'C02010034-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '630', 'price_USD' => '690', 'price_GBP' => '580', 'price_JPY' => '98690', 'price_CNY' => '', 'price_AUD' => '1725', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns', 'meta_title' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'meta_keywords' => '', 'meta_description' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'modified' => '2021-01-18 10:37:19', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '759', 'product_id' => '1911', 'related_id' => '1885' ), 'Image' => array( (int) 0 => array( 'id' => '1778', 'name' => 'product/kits/methyl-kit-icon.png', 'alt' => 'Methylation kit icon', 'modified' => '2019-04-23 15:17:01', 'created' => '2018-03-15 15:52:12', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(kch-821-660)</span>' $country_code = 'US' $other_format = array( 'id' => '1912', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '660 µl', 'catalog_number' => 'C03010022-660', 'old_catalog_number' => 'kch-821-660', 'sf_code' => 'C03010022-350', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '265', 'price_USD' => '250', 'price_GBP' => '245', 'price_JPY' => '41510', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-660-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:02', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '179', 'product_id' => '1912', 'group_id' => '167' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '911', 'name' => 'DiaMag anti-mouse IgG-coated magnetic beads', 'description' => '<p>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP).</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/c03010022-kch-821.pdf', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-19 10:45:17', 'created' => '2016-07-19 10:45:17', 'ProductsDocument' => array( 'id' => '2177', 'product_id' => '1911', 'document_id' => '911' ) ) $sds = array( 'id' => '332', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS ES es', 'language' => 'es', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 13:00:23', 'created' => '2020-06-09 13:00:23', 'ProductsSafetySheet' => array( 'id' => '650', 'product_id' => '1911', 'safety_sheet_id' => '332' ) ) $publication = array( 'id' => '3598', 'name' => 'Novel dual regulators of Pseudomonas aeruginosa essential for productive biofilms and virulence.', 'authors' => 'Heacock-Kang Y, Zarzycki-Siek J, Sun Z, Poonsuk K, Bluhm AP, Cabanas D, Fogen D, McMillan IA, Chuanchuen R, Hoang TT', 'description' => '<p>Gene regulation network in Pseudomonas aeruginosa is complex. With a relatively large genome (6.2 Mb), there is a significant portion of genes that are proven or predicted to be transcriptional regulators. Many of these regulators have been shown to play important roles in biofilm formation and maintenance. In this study, we present a novel transcriptional regulator, PA1226, which modulates biofilm formation and virulence in P. aeruginosa. Mutation in the gene encoding this regulator abolished the ability of P. aeruginosa to produce biofilms in vitro, without any effect on the planktonic growth. This regulator is also essential for the in vivo fitness and pathogenesis in both Drosophila melanogaster and BALB/c mouse lung infection models. Transcriptome analysis revealed that PA1226 regulates many essential virulence genes/pathways, including those involved in alginate, pili, and LPS biosynthesis. Genes/operons directly regulated by PA1226 and potential binding sequences were identified via ChIP-seq. Attempts to confirm the binding sequences by electrophoretic mobility shift assay led to the discovery of a co-regulator, PA1413, via co-immunoprecipitation assay. PA1226 and PA1413 were shown to bind collaboratively to the promoter regions of their regulons. A model is proposed, summarizing our finding on this novel dual-regulation system.</p>', 'date' => '2018-08-01', 'pmid' => 'http://www.pubmed.gov/29995308', 'doi' => '10.1111/mmi.14063', 'modified' => '2019-04-17 15:07:51', 'created' => '2019-04-16 12:25:30', 'ProductsPublication' => array( 'id' => '3470', 'product_id' => '1911', 'publication_id' => '3598' ) ) $externalLink = ' <a href="http://www.pubmed.gov/29995308" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'product' => array( 'Product' => array( 'id' => '1911', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '220 µl', 'catalog_number' => 'C03010022-220', 'old_catalog_number' => 'kch-821-220', 'sf_code' => 'C03010022-D001-000572', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '100', 'price_USD' => '125', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '312', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-220-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:27', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/diamag-anti-mouse-igg-coated-magnetic-beads-220-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'DiaMag anti-mouse IgG coated magnetic beads' $meta_title = 'DiaMag anti-mouse IgG coated magnetic beads' $product = array( 'Product' => array( 'id' => '1911', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '220 µl', 'catalog_number' => 'C03010022-220', 'old_catalog_number' => 'kch-821-220', 'sf_code' => 'C03010022-D001-000572', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '100', 'price_USD' => '125', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '312', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-220-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:27', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '167', 'name' => 'C03010022', 'product_id' => '1911', 'modified' => '2016-02-23 20:52:11', 'created' => '2016-02-23 20:52:11' ) ), 'Group' => array( 'Group' => array( 'id' => '167', 'name' => 'C03010022', 'product_id' => '1911', 'modified' => '2016-02-23 20:52:11', 'created' => '2016-02-23 20:52:11' ), 'Master' => array( 'id' => '1911', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '220 µl', 'catalog_number' => 'C03010022-220', 'old_catalog_number' => 'kch-821-220', 'sf_code' => 'C03010022-D001-000572', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '100', 'price_USD' => '125', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '312', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-220-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:27', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '1885', 'antibody_id' => null, 'name' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'description' => '<p><span>The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation analysis. It features</span><span> a highly specific monoclonal antibody against </span><span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA</span><span>. It includes control DNA and primers to assess the effiency of the assay. </span><span>Performing hydroxymethylation profiling with the hMeDIP kit is fast, reliable and highly specific.</span></p>', 'label1' => ' Characteristics', 'info1' => '<ul style="list-style-type: disc;"> <li><span>Robust enrichment & immunoprecipitation of hydroxymethylated DNA</span></li> <li>Highly specific monoclonal antibody against 5-hmC<span> for reliable, reproducible results</span></li> <li>Including control DNA and primers to <span>monitor the efficiency of the assay</span> <ul style="list-style-type: circle;"> <li>5-hmC, 5-mC and unmethylated DNA sequences and primer pairs</li> <li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li> </ul> </li> </ul> <ul style="list-style-type: disc;"> <li>Improved single-tube, magnetic bead-based protocol</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '16 rxns', 'catalog_number' => 'C02010034', 'old_catalog_number' => '', 'sf_code' => 'C02010034-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '630', 'price_USD' => '690', 'price_GBP' => '580', 'price_JPY' => '98690', 'price_CNY' => '', 'price_AUD' => '1725', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns', 'meta_title' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'meta_keywords' => '', 'meta_description' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'modified' => '2021-01-18 10:37:19', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '46', 'position' => '1', 'parent_id' => '34', 'name' => 'Magnetic beads', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'magnetic-beads', 'cookies_tag_id' => null, 'meta_keywords' => 'Magnetic beads,chromatin immunoprecipitation assay,DNA immunoprecipitation ', 'meta_description' => 'Diagenode's Magnetic beads are extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP)', 'meta_title' => 'Magnetic beads for ChIP assay | DIagenode', 'modified' => '2016-02-24 15:05:50', 'created' => '2015-07-07 16:53:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '911', 'name' => 'DiaMag anti-mouse IgG-coated magnetic beads', 'description' => '<p>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP).</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/c03010022-kch-821.pdf', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-19 10:45:17', 'created' => '2016-07-19 10:45:17', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4320', 'name' => 'Identification of a PadR-type regulator essential for intracellularpathogenesis of', 'authors' => 'McMillan Ian A. et al. ', 'description' => '<p>Burkholderia pseudomallei (Bp) is the causative agent of melioidosis, a disease endemic to the tropics. Melioidosis manifests in various ways ranging from acute skin lesions to pneumonia and, in rare cases, infection of the central nervous system. Bp is a facultative intracellular pathogen and it can infect various cell types. The Bp intracellular lifecycle has been partially elucidated and is highly complex. Herein, we have identified a transcriptional regulator, BP1026B_II1198, that is differentially expressed as Bp transits through host cells. A deletion mutant of BP1026B_II1198 was attenuated in RAW264.7 cell and BALB/c mouse infection. To further characterize the function of this transcriptional regulator, we endeavored to determine the regulon of BP1026B_II1198. RNA-seq analysis showed the global picture of genes regulated while ChIP-seq analysis identified two specific BP1026B_II1198 binding regions on chromosome II. We investigated the transposon mutants of these genes controlled by BP1026B_II1198 and confirmed that these genes contribute to pathogenesis in RAW264.7 murine macrophage cells. Taken together, the data presented here shed light on the regulon of BP1026B_II1198 and its role during intracellular infection and highlights an integral portion of the highly complex regulation network of Bp during host infection.</p>', 'date' => '2021-05-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34001967', 'doi' => '10.1038/s41598-021-89852-7', 'modified' => '2022-08-02 16:55:52', 'created' => '2022-05-19 10:41:50', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '4092', 'name' => 'Formation of the CenH3-Deficient Holocentromere in Lepidoptera AvoidsActive Chromatin.', 'authors' => 'Senaratne, Aruni P and Muller, Héloïse and Fryer, Kelsey A and Kawamoto,Munetaka and Katsuma, Susumu and Drinnenberg, Ines A', 'description' => '<p>Despite the essentiality for faithful chromosome segregation, centromere architectures are diverse among eukaryotes and embody two main configurations: mono- and holocentromeres, referring, respectively, to localized or unrestricted distribution of centromeric activity. Of the two, some holocentromeres offer the curious condition of having arisen independently in multiple insects, most of which have lost the otherwise essential centromere-specifying factor CenH3 (first described as CENP-A in humans). The loss of CenH3 raises intuitive questions about how holocentromeres are organized and regulated in CenH3-lacking insects. Here, we report the first chromatin-level description of CenH3-deficient holocentromeres by leveraging recently identified centromere components and genomics approaches to map and characterize the holocentromeres of the silk moth Bombyx mori, a representative lepidopteran insect lacking CenH3. This uncovered a robust correlation between the distribution of centromere sites and regions of low chromatin activity along B. mori chromosomes. Transcriptional perturbation experiments recapitulated the exclusion of B. mori centromeres from active chromatin. Based on reciprocal centromere occupancy patterns observed along differentially expressed orthologous genes of Lepidoptera, we further found that holocentromere formation in a manner that is recessive to chromatin dynamics is evolutionarily conserved. Our results help us discuss the plasticity of centromeres in the context of a role for the chromosome-wide chromatin landscape in conferring centromere identity rather than the presence of CenH3. Given the co-occurrence of CenH3 loss and holocentricity in insects, we further propose that the evolutionary establishment of holocentromeres in insects was facilitated through the loss of a CenH3-specified centromere.</p>', 'date' => '2020-10-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33125865', 'doi' => '10.1016/j.cub.2020.09.078', 'modified' => '2021-03-17 17:13:50', 'created' => '2021-02-18 10:21:53', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3598', 'name' => 'Novel dual regulators of Pseudomonas aeruginosa essential for productive biofilms and virulence.', 'authors' => 'Heacock-Kang Y, Zarzycki-Siek J, Sun Z, Poonsuk K, Bluhm AP, Cabanas D, Fogen D, McMillan IA, Chuanchuen R, Hoang TT', 'description' => '<p>Gene regulation network in Pseudomonas aeruginosa is complex. With a relatively large genome (6.2 Mb), there is a significant portion of genes that are proven or predicted to be transcriptional regulators. Many of these regulators have been shown to play important roles in biofilm formation and maintenance. In this study, we present a novel transcriptional regulator, PA1226, which modulates biofilm formation and virulence in P. aeruginosa. Mutation in the gene encoding this regulator abolished the ability of P. aeruginosa to produce biofilms in vitro, without any effect on the planktonic growth. This regulator is also essential for the in vivo fitness and pathogenesis in both Drosophila melanogaster and BALB/c mouse lung infection models. Transcriptome analysis revealed that PA1226 regulates many essential virulence genes/pathways, including those involved in alginate, pili, and LPS biosynthesis. Genes/operons directly regulated by PA1226 and potential binding sequences were identified via ChIP-seq. Attempts to confirm the binding sequences by electrophoretic mobility shift assay led to the discovery of a co-regulator, PA1413, via co-immunoprecipitation assay. PA1226 and PA1413 were shown to bind collaboratively to the promoter regions of their regulons. A model is proposed, summarizing our finding on this novel dual-regulation system.</p>', 'date' => '2018-08-01', 'pmid' => 'http://www.pubmed.gov/29995308', 'doi' => '10.1111/mmi.14063', 'modified' => '2019-04-17 15:07:51', 'created' => '2019-04-16 12:25:30', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '334', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS GB en', 'language' => 'en', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-GB-en-GHS_1_0.pdf', 'countries' => 'GB', 'modified' => '2020-06-09 13:01:35', 'created' => '2020-06-09 13:01:35', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '336', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS US en', 'language' => 'en', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-US-en-GHS_1_0.pdf', 'countries' => 'US', 'modified' => '2020-06-09 13:03:27', 'created' => '2020-06-09 13:03:27', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '331', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS DE de', 'language' => 'de', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-DE-de-GHS_1_0.pdf', 'countries' => 'DE', 'modified' => '2020-06-09 12:59:50', 'created' => '2020-06-09 12:59:50', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '335', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-06-09 13:02:44', 'created' => '2020-06-09 13:02:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '330', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-BE-nl-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 12:59:19', 'created' => '2020-06-09 12:59:19', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '329', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-BE-fr-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 12:58:49', 'created' => '2020-06-09 12:58:49', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '333', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-FR-fr-GHS_1_0.pdf', 'countries' => 'FR', 'modified' => '2020-06-09 13:00:53', 'created' => '2020-06-09 13:00:53', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '332', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS ES es', 'language' => 'es', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 13:00:23', 'created' => '2020-06-09 13:00:23', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/diamag-anti-mouse-igg-coated-magnetic-beads-220-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '1910', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1.5 ml', 'catalog_number' => 'C03010022-150', 'old_catalog_number' => 'kch-821-150', 'sf_code' => 'C03010022-351', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '575', 'price_USD' => '405', 'price_GBP' => '520', 'price_JPY' => '90075', 'price_CNY' => '', 'price_AUD' => '1012', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-1-5-ml', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2016-07-19 10:45:27', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '178', 'product_id' => '1910', 'group_id' => '167' ) ), (int) 1 => array( 'id' => '1912', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '660 µl', 'catalog_number' => 'C03010022-660', 'old_catalog_number' => 'kch-821-660', 'sf_code' => 'C03010022-350', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '265', 'price_USD' => '250', 'price_GBP' => '245', 'price_JPY' => '41510', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-660-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:02', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '179', 'product_id' => '1912', 'group_id' => '167' ) ) ) $pro = array( 'id' => '1912', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '660 µl', 'catalog_number' => 'C03010022-660', 'old_catalog_number' => 'kch-821-660', 'sf_code' => 'C03010022-350', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '265', 'price_USD' => '250', 'price_GBP' => '245', 'price_JPY' => '41510', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-660-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:02', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '179', 'product_id' => '1912', 'group_id' => '167' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C02010034</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1885" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/1885" id="CartAdd/1885Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1885" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto hMeDIP kit x16 (monoclonal mouse antibody)</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'C02010034', '690', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'C02010034', '690', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns" data-reveal-id="cartModal-1885" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Auto hMeDIP kit x16 (monoclonal mouse antibody)</h6> </div> </div> </li> ' $related = array( 'id' => '1885', 'antibody_id' => null, 'name' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'description' => '<p><span>The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation analysis. It features</span><span> a highly specific monoclonal antibody against </span><span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA</span><span>. It includes control DNA and primers to assess the effiency of the assay. </span><span>Performing hydroxymethylation profiling with the hMeDIP kit is fast, reliable and highly specific.</span></p>', 'label1' => ' Characteristics', 'info1' => '<ul style="list-style-type: disc;"> <li><span>Robust enrichment & immunoprecipitation of hydroxymethylated DNA</span></li> <li>Highly specific monoclonal antibody against 5-hmC<span> for reliable, reproducible results</span></li> <li>Including control DNA and primers to <span>monitor the efficiency of the assay</span> <ul style="list-style-type: circle;"> <li>5-hmC, 5-mC and unmethylated DNA sequences and primer pairs</li> <li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li> </ul> </li> </ul> <ul style="list-style-type: disc;"> <li>Improved single-tube, magnetic bead-based protocol</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '16 rxns', 'catalog_number' => 'C02010034', 'old_catalog_number' => '', 'sf_code' => 'C02010034-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '630', 'price_USD' => '690', 'price_GBP' => '580', 'price_JPY' => '98690', 'price_CNY' => '', 'price_AUD' => '1725', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns', 'meta_title' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'meta_keywords' => '', 'meta_description' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'modified' => '2021-01-18 10:37:19', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '759', 'product_id' => '1911', 'related_id' => '1885' ), 'Image' => array( (int) 0 => array( 'id' => '1778', 'name' => 'product/kits/methyl-kit-icon.png', 'alt' => 'Methylation kit icon', 'modified' => '2019-04-23 15:17:01', 'created' => '2018-03-15 15:52:12', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(kch-821-660)</span>' $country_code = 'US' $other_format = array( 'id' => '1912', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '660 µl', 'catalog_number' => 'C03010022-660', 'old_catalog_number' => 'kch-821-660', 'sf_code' => 'C03010022-350', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '265', 'price_USD' => '250', 'price_GBP' => '245', 'price_JPY' => '41510', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-660-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:02', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '179', 'product_id' => '1912', 'group_id' => '167' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '911', 'name' => 'DiaMag anti-mouse IgG-coated magnetic beads', 'description' => '<p>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP).</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/c03010022-kch-821.pdf', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-19 10:45:17', 'created' => '2016-07-19 10:45:17', 'ProductsDocument' => array( 'id' => '2177', 'product_id' => '1911', 'document_id' => '911' ) ) $sds = array( 'id' => '332', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS ES es', 'language' => 'es', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 13:00:23', 'created' => '2020-06-09 13:00:23', 'ProductsSafetySheet' => array( 'id' => '650', 'product_id' => '1911', 'safety_sheet_id' => '332' ) ) $publication = array( 'id' => '3598', 'name' => 'Novel dual regulators of Pseudomonas aeruginosa essential for productive biofilms and virulence.', 'authors' => 'Heacock-Kang Y, Zarzycki-Siek J, Sun Z, Poonsuk K, Bluhm AP, Cabanas D, Fogen D, McMillan IA, Chuanchuen R, Hoang TT', 'description' => '<p>Gene regulation network in Pseudomonas aeruginosa is complex. With a relatively large genome (6.2 Mb), there is a significant portion of genes that are proven or predicted to be transcriptional regulators. Many of these regulators have been shown to play important roles in biofilm formation and maintenance. In this study, we present a novel transcriptional regulator, PA1226, which modulates biofilm formation and virulence in P. aeruginosa. Mutation in the gene encoding this regulator abolished the ability of P. aeruginosa to produce biofilms in vitro, without any effect on the planktonic growth. This regulator is also essential for the in vivo fitness and pathogenesis in both Drosophila melanogaster and BALB/c mouse lung infection models. Transcriptome analysis revealed that PA1226 regulates many essential virulence genes/pathways, including those involved in alginate, pili, and LPS biosynthesis. Genes/operons directly regulated by PA1226 and potential binding sequences were identified via ChIP-seq. Attempts to confirm the binding sequences by electrophoretic mobility shift assay led to the discovery of a co-regulator, PA1413, via co-immunoprecipitation assay. PA1226 and PA1413 were shown to bind collaboratively to the promoter regions of their regulons. A model is proposed, summarizing our finding on this novel dual-regulation system.</p>', 'date' => '2018-08-01', 'pmid' => 'http://www.pubmed.gov/29995308', 'doi' => '10.1111/mmi.14063', 'modified' => '2019-04-17 15:07:51', 'created' => '2019-04-16 12:25:30', 'ProductsPublication' => array( 'id' => '3470', 'product_id' => '1911', 'publication_id' => '3598' ) ) $externalLink = ' <a href="http://www.pubmed.gov/29995308" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'product' => array( 'Product' => array( 'id' => '1911', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '220 µl', 'catalog_number' => 'C03010022-220', 'old_catalog_number' => 'kch-821-220', 'sf_code' => 'C03010022-D001-000572', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '100', 'price_USD' => '125', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '312', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-220-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:27', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/diamag-anti-mouse-igg-coated-magnetic-beads-220-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'DiaMag anti-mouse IgG coated magnetic beads' $meta_title = 'DiaMag anti-mouse IgG coated magnetic beads' $product = array( 'Product' => array( 'id' => '1911', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '220 µl', 'catalog_number' => 'C03010022-220', 'old_catalog_number' => 'kch-821-220', 'sf_code' => 'C03010022-D001-000572', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '100', 'price_USD' => '125', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '312', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-220-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:27', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '167', 'name' => 'C03010022', 'product_id' => '1911', 'modified' => '2016-02-23 20:52:11', 'created' => '2016-02-23 20:52:11' ) ), 'Group' => array( 'Group' => array( 'id' => '167', 'name' => 'C03010022', 'product_id' => '1911', 'modified' => '2016-02-23 20:52:11', 'created' => '2016-02-23 20:52:11' ), 'Master' => array( 'id' => '1911', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '220 µl', 'catalog_number' => 'C03010022-220', 'old_catalog_number' => 'kch-821-220', 'sf_code' => 'C03010022-D001-000572', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '100', 'price_USD' => '125', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '312', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-220-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:27', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '1885', 'antibody_id' => null, 'name' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'description' => '<p><span>The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation analysis. It features</span><span> a highly specific monoclonal antibody against </span><span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA</span><span>. It includes control DNA and primers to assess the effiency of the assay. </span><span>Performing hydroxymethylation profiling with the hMeDIP kit is fast, reliable and highly specific.</span></p>', 'label1' => ' Characteristics', 'info1' => '<ul style="list-style-type: disc;"> <li><span>Robust enrichment & immunoprecipitation of hydroxymethylated DNA</span></li> <li>Highly specific monoclonal antibody against 5-hmC<span> for reliable, reproducible results</span></li> <li>Including control DNA and primers to <span>monitor the efficiency of the assay</span> <ul style="list-style-type: circle;"> <li>5-hmC, 5-mC and unmethylated DNA sequences and primer pairs</li> <li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li> </ul> </li> </ul> <ul style="list-style-type: disc;"> <li>Improved single-tube, magnetic bead-based protocol</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '16 rxns', 'catalog_number' => 'C02010034', 'old_catalog_number' => '', 'sf_code' => 'C02010034-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '630', 'price_USD' => '690', 'price_GBP' => '580', 'price_JPY' => '98690', 'price_CNY' => '', 'price_AUD' => '1725', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns', 'meta_title' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'meta_keywords' => '', 'meta_description' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'modified' => '2021-01-18 10:37:19', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '46', 'position' => '1', 'parent_id' => '34', 'name' => 'Magnetic beads', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'magnetic-beads', 'cookies_tag_id' => null, 'meta_keywords' => 'Magnetic beads,chromatin immunoprecipitation assay,DNA immunoprecipitation ', 'meta_description' => 'Diagenode's Magnetic beads are extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP)', 'meta_title' => 'Magnetic beads for ChIP assay | DIagenode', 'modified' => '2016-02-24 15:05:50', 'created' => '2015-07-07 16:53:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '911', 'name' => 'DiaMag anti-mouse IgG-coated magnetic beads', 'description' => '<p>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP).</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/c03010022-kch-821.pdf', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-19 10:45:17', 'created' => '2016-07-19 10:45:17', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4320', 'name' => 'Identification of a PadR-type regulator essential for intracellularpathogenesis of', 'authors' => 'McMillan Ian A. et al. ', 'description' => '<p>Burkholderia pseudomallei (Bp) is the causative agent of melioidosis, a disease endemic to the tropics. Melioidosis manifests in various ways ranging from acute skin lesions to pneumonia and, in rare cases, infection of the central nervous system. Bp is a facultative intracellular pathogen and it can infect various cell types. The Bp intracellular lifecycle has been partially elucidated and is highly complex. Herein, we have identified a transcriptional regulator, BP1026B_II1198, that is differentially expressed as Bp transits through host cells. A deletion mutant of BP1026B_II1198 was attenuated in RAW264.7 cell and BALB/c mouse infection. To further characterize the function of this transcriptional regulator, we endeavored to determine the regulon of BP1026B_II1198. RNA-seq analysis showed the global picture of genes regulated while ChIP-seq analysis identified two specific BP1026B_II1198 binding regions on chromosome II. We investigated the transposon mutants of these genes controlled by BP1026B_II1198 and confirmed that these genes contribute to pathogenesis in RAW264.7 murine macrophage cells. Taken together, the data presented here shed light on the regulon of BP1026B_II1198 and its role during intracellular infection and highlights an integral portion of the highly complex regulation network of Bp during host infection.</p>', 'date' => '2021-05-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34001967', 'doi' => '10.1038/s41598-021-89852-7', 'modified' => '2022-08-02 16:55:52', 'created' => '2022-05-19 10:41:50', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '4092', 'name' => 'Formation of the CenH3-Deficient Holocentromere in Lepidoptera AvoidsActive Chromatin.', 'authors' => 'Senaratne, Aruni P and Muller, Héloïse and Fryer, Kelsey A and Kawamoto,Munetaka and Katsuma, Susumu and Drinnenberg, Ines A', 'description' => '<p>Despite the essentiality for faithful chromosome segregation, centromere architectures are diverse among eukaryotes and embody two main configurations: mono- and holocentromeres, referring, respectively, to localized or unrestricted distribution of centromeric activity. Of the two, some holocentromeres offer the curious condition of having arisen independently in multiple insects, most of which have lost the otherwise essential centromere-specifying factor CenH3 (first described as CENP-A in humans). The loss of CenH3 raises intuitive questions about how holocentromeres are organized and regulated in CenH3-lacking insects. Here, we report the first chromatin-level description of CenH3-deficient holocentromeres by leveraging recently identified centromere components and genomics approaches to map and characterize the holocentromeres of the silk moth Bombyx mori, a representative lepidopteran insect lacking CenH3. This uncovered a robust correlation between the distribution of centromere sites and regions of low chromatin activity along B. mori chromosomes. Transcriptional perturbation experiments recapitulated the exclusion of B. mori centromeres from active chromatin. Based on reciprocal centromere occupancy patterns observed along differentially expressed orthologous genes of Lepidoptera, we further found that holocentromere formation in a manner that is recessive to chromatin dynamics is evolutionarily conserved. Our results help us discuss the plasticity of centromeres in the context of a role for the chromosome-wide chromatin landscape in conferring centromere identity rather than the presence of CenH3. Given the co-occurrence of CenH3 loss and holocentricity in insects, we further propose that the evolutionary establishment of holocentromeres in insects was facilitated through the loss of a CenH3-specified centromere.</p>', 'date' => '2020-10-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33125865', 'doi' => '10.1016/j.cub.2020.09.078', 'modified' => '2021-03-17 17:13:50', 'created' => '2021-02-18 10:21:53', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3598', 'name' => 'Novel dual regulators of Pseudomonas aeruginosa essential for productive biofilms and virulence.', 'authors' => 'Heacock-Kang Y, Zarzycki-Siek J, Sun Z, Poonsuk K, Bluhm AP, Cabanas D, Fogen D, McMillan IA, Chuanchuen R, Hoang TT', 'description' => '<p>Gene regulation network in Pseudomonas aeruginosa is complex. With a relatively large genome (6.2 Mb), there is a significant portion of genes that are proven or predicted to be transcriptional regulators. Many of these regulators have been shown to play important roles in biofilm formation and maintenance. In this study, we present a novel transcriptional regulator, PA1226, which modulates biofilm formation and virulence in P. aeruginosa. Mutation in the gene encoding this regulator abolished the ability of P. aeruginosa to produce biofilms in vitro, without any effect on the planktonic growth. This regulator is also essential for the in vivo fitness and pathogenesis in both Drosophila melanogaster and BALB/c mouse lung infection models. Transcriptome analysis revealed that PA1226 regulates many essential virulence genes/pathways, including those involved in alginate, pili, and LPS biosynthesis. Genes/operons directly regulated by PA1226 and potential binding sequences were identified via ChIP-seq. Attempts to confirm the binding sequences by electrophoretic mobility shift assay led to the discovery of a co-regulator, PA1413, via co-immunoprecipitation assay. PA1226 and PA1413 were shown to bind collaboratively to the promoter regions of their regulons. A model is proposed, summarizing our finding on this novel dual-regulation system.</p>', 'date' => '2018-08-01', 'pmid' => 'http://www.pubmed.gov/29995308', 'doi' => '10.1111/mmi.14063', 'modified' => '2019-04-17 15:07:51', 'created' => '2019-04-16 12:25:30', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '334', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS GB en', 'language' => 'en', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-GB-en-GHS_1_0.pdf', 'countries' => 'GB', 'modified' => '2020-06-09 13:01:35', 'created' => '2020-06-09 13:01:35', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '336', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS US en', 'language' => 'en', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-US-en-GHS_1_0.pdf', 'countries' => 'US', 'modified' => '2020-06-09 13:03:27', 'created' => '2020-06-09 13:03:27', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '331', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS DE de', 'language' => 'de', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-DE-de-GHS_1_0.pdf', 'countries' => 'DE', 'modified' => '2020-06-09 12:59:50', 'created' => '2020-06-09 12:59:50', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '335', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-06-09 13:02:44', 'created' => '2020-06-09 13:02:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '330', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-BE-nl-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 12:59:19', 'created' => '2020-06-09 12:59:19', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '329', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-BE-fr-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 12:58:49', 'created' => '2020-06-09 12:58:49', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '333', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-FR-fr-GHS_1_0.pdf', 'countries' => 'FR', 'modified' => '2020-06-09 13:00:53', 'created' => '2020-06-09 13:00:53', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '332', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS ES es', 'language' => 'es', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 13:00:23', 'created' => '2020-06-09 13:00:23', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/diamag-anti-mouse-igg-coated-magnetic-beads-220-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '1910', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1.5 ml', 'catalog_number' => 'C03010022-150', 'old_catalog_number' => 'kch-821-150', 'sf_code' => 'C03010022-351', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '575', 'price_USD' => '405', 'price_GBP' => '520', 'price_JPY' => '90075', 'price_CNY' => '', 'price_AUD' => '1012', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-1-5-ml', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2016-07-19 10:45:27', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '178', 'product_id' => '1910', 'group_id' => '167' ) ), (int) 1 => array( 'id' => '1912', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '660 µl', 'catalog_number' => 'C03010022-660', 'old_catalog_number' => 'kch-821-660', 'sf_code' => 'C03010022-350', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '265', 'price_USD' => '250', 'price_GBP' => '245', 'price_JPY' => '41510', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-660-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:02', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '179', 'product_id' => '1912', 'group_id' => '167' ) ) ) $pro = array( 'id' => '1912', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '660 µl', 'catalog_number' => 'C03010022-660', 'old_catalog_number' => 'kch-821-660', 'sf_code' => 'C03010022-350', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '265', 'price_USD' => '250', 'price_GBP' => '245', 'price_JPY' => '41510', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-660-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:02', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '179', 'product_id' => '1912', 'group_id' => '167' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C02010034</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1885" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/1885" id="CartAdd/1885Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1885" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto hMeDIP kit x16 (monoclonal mouse antibody)</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'C02010034', '690', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'C02010034', '690', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns" data-reveal-id="cartModal-1885" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Auto hMeDIP kit x16 (monoclonal mouse antibody)</h6> </div> </div> </li> ' $related = array( 'id' => '1885', 'antibody_id' => null, 'name' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'description' => '<p><span>The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation analysis. It features</span><span> a highly specific monoclonal antibody against </span><span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA</span><span>. It includes control DNA and primers to assess the effiency of the assay. </span><span>Performing hydroxymethylation profiling with the hMeDIP kit is fast, reliable and highly specific.</span></p>', 'label1' => ' Characteristics', 'info1' => '<ul style="list-style-type: disc;"> <li><span>Robust enrichment & immunoprecipitation of hydroxymethylated DNA</span></li> <li>Highly specific monoclonal antibody against 5-hmC<span> for reliable, reproducible results</span></li> <li>Including control DNA and primers to <span>monitor the efficiency of the assay</span> <ul style="list-style-type: circle;"> <li>5-hmC, 5-mC and unmethylated DNA sequences and primer pairs</li> <li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li> </ul> </li> </ul> <ul style="list-style-type: disc;"> <li>Improved single-tube, magnetic bead-based protocol</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '16 rxns', 'catalog_number' => 'C02010034', 'old_catalog_number' => '', 'sf_code' => 'C02010034-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '630', 'price_USD' => '690', 'price_GBP' => '580', 'price_JPY' => '98690', 'price_CNY' => '', 'price_AUD' => '1725', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns', 'meta_title' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'meta_keywords' => '', 'meta_description' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'modified' => '2021-01-18 10:37:19', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '759', 'product_id' => '1911', 'related_id' => '1885' ), 'Image' => array( (int) 0 => array( 'id' => '1778', 'name' => 'product/kits/methyl-kit-icon.png', 'alt' => 'Methylation kit icon', 'modified' => '2019-04-23 15:17:01', 'created' => '2018-03-15 15:52:12', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(kch-821-660)</span>' $country_code = 'US' $other_format = array( 'id' => '1912', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '660 µl', 'catalog_number' => 'C03010022-660', 'old_catalog_number' => 'kch-821-660', 'sf_code' => 'C03010022-350', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '265', 'price_USD' => '250', 'price_GBP' => '245', 'price_JPY' => '41510', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-660-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:02', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '179', 'product_id' => '1912', 'group_id' => '167' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '911', 'name' => 'DiaMag anti-mouse IgG-coated magnetic beads', 'description' => '<p>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP).</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/c03010022-kch-821.pdf', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-19 10:45:17', 'created' => '2016-07-19 10:45:17', 'ProductsDocument' => array( 'id' => '2177', 'product_id' => '1911', 'document_id' => '911' ) ) $sds = array( 'id' => '332', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS ES es', 'language' => 'es', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 13:00:23', 'created' => '2020-06-09 13:00:23', 'ProductsSafetySheet' => array( 'id' => '650', 'product_id' => '1911', 'safety_sheet_id' => '332' ) ) $publication = array( 'id' => '3598', 'name' => 'Novel dual regulators of Pseudomonas aeruginosa essential for productive biofilms and virulence.', 'authors' => 'Heacock-Kang Y, Zarzycki-Siek J, Sun Z, Poonsuk K, Bluhm AP, Cabanas D, Fogen D, McMillan IA, Chuanchuen R, Hoang TT', 'description' => '<p>Gene regulation network in Pseudomonas aeruginosa is complex. With a relatively large genome (6.2 Mb), there is a significant portion of genes that are proven or predicted to be transcriptional regulators. Many of these regulators have been shown to play important roles in biofilm formation and maintenance. In this study, we present a novel transcriptional regulator, PA1226, which modulates biofilm formation and virulence in P. aeruginosa. Mutation in the gene encoding this regulator abolished the ability of P. aeruginosa to produce biofilms in vitro, without any effect on the planktonic growth. This regulator is also essential for the in vivo fitness and pathogenesis in both Drosophila melanogaster and BALB/c mouse lung infection models. Transcriptome analysis revealed that PA1226 regulates many essential virulence genes/pathways, including those involved in alginate, pili, and LPS biosynthesis. Genes/operons directly regulated by PA1226 and potential binding sequences were identified via ChIP-seq. Attempts to confirm the binding sequences by electrophoretic mobility shift assay led to the discovery of a co-regulator, PA1413, via co-immunoprecipitation assay. PA1226 and PA1413 were shown to bind collaboratively to the promoter regions of their regulons. A model is proposed, summarizing our finding on this novel dual-regulation system.</p>', 'date' => '2018-08-01', 'pmid' => 'http://www.pubmed.gov/29995308', 'doi' => '10.1111/mmi.14063', 'modified' => '2019-04-17 15:07:51', 'created' => '2019-04-16 12:25:30', 'ProductsPublication' => array( 'id' => '3470', 'product_id' => '1911', 'publication_id' => '3598' ) ) $externalLink = ' <a href="http://www.pubmed.gov/29995308" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'product' => array( 'Product' => array( 'id' => '1911', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '220 µl', 'catalog_number' => 'C03010022-220', 'old_catalog_number' => 'kch-821-220', 'sf_code' => 'C03010022-D001-000572', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '100', 'price_USD' => '125', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '312', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-220-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:27', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array( (int) 0 => array( [maximum depth reached] ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/diamag-anti-mouse-igg-coated-magnetic-beads-220-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'DiaMag anti-mouse IgG coated magnetic beads' $meta_title = 'DiaMag anti-mouse IgG coated magnetic beads' $product = array( 'Product' => array( 'id' => '1911', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '220 µl', 'catalog_number' => 'C03010022-220', 'old_catalog_number' => 'kch-821-220', 'sf_code' => 'C03010022-D001-000572', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '100', 'price_USD' => '125', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '312', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-220-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:27', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '167', 'name' => 'C03010022', 'product_id' => '1911', 'modified' => '2016-02-23 20:52:11', 'created' => '2016-02-23 20:52:11' ) ), 'Group' => array( 'Group' => array( 'id' => '167', 'name' => 'C03010022', 'product_id' => '1911', 'modified' => '2016-02-23 20:52:11', 'created' => '2016-02-23 20:52:11' ), 'Master' => array( 'id' => '1911', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '220 µl', 'catalog_number' => 'C03010022-220', 'old_catalog_number' => 'kch-821-220', 'sf_code' => 'C03010022-D001-000572', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '100', 'price_USD' => '125', 'price_GBP' => '95', 'price_JPY' => '15665', 'price_CNY' => '', 'price_AUD' => '312', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-220-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:27', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ) ), 'Related' => array( (int) 0 => array( 'id' => '1885', 'antibody_id' => null, 'name' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'description' => '<p><span>The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation analysis. It features</span><span> a highly specific monoclonal antibody against </span><span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA</span><span>. It includes control DNA and primers to assess the effiency of the assay. </span><span>Performing hydroxymethylation profiling with the hMeDIP kit is fast, reliable and highly specific.</span></p>', 'label1' => ' Characteristics', 'info1' => '<ul style="list-style-type: disc;"> <li><span>Robust enrichment & immunoprecipitation of hydroxymethylated DNA</span></li> <li>Highly specific monoclonal antibody against 5-hmC<span> for reliable, reproducible results</span></li> <li>Including control DNA and primers to <span>monitor the efficiency of the assay</span> <ul style="list-style-type: circle;"> <li>5-hmC, 5-mC and unmethylated DNA sequences and primer pairs</li> <li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li> </ul> </li> </ul> <ul style="list-style-type: disc;"> <li>Improved single-tube, magnetic bead-based protocol</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '16 rxns', 'catalog_number' => 'C02010034', 'old_catalog_number' => '', 'sf_code' => 'C02010034-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '630', 'price_USD' => '690', 'price_GBP' => '580', 'price_JPY' => '98690', 'price_CNY' => '', 'price_AUD' => '1725', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns', 'meta_title' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'meta_keywords' => '', 'meta_description' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'modified' => '2021-01-18 10:37:19', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '46', 'position' => '1', 'parent_id' => '34', 'name' => 'Magnetic beads', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'magnetic-beads', 'cookies_tag_id' => null, 'meta_keywords' => 'Magnetic beads,chromatin immunoprecipitation assay,DNA immunoprecipitation ', 'meta_description' => 'Diagenode's Magnetic beads are extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP)', 'meta_title' => 'Magnetic beads for ChIP assay | DIagenode', 'modified' => '2016-02-24 15:05:50', 'created' => '2015-07-07 16:53:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '911', 'name' => 'DiaMag anti-mouse IgG-coated magnetic beads', 'description' => '<p>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP).</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/c03010022-kch-821.pdf', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-19 10:45:17', 'created' => '2016-07-19 10:45:17', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '4320', 'name' => 'Identification of a PadR-type regulator essential for intracellularpathogenesis of', 'authors' => 'McMillan Ian A. et al. ', 'description' => '<p>Burkholderia pseudomallei (Bp) is the causative agent of melioidosis, a disease endemic to the tropics. Melioidosis manifests in various ways ranging from acute skin lesions to pneumonia and, in rare cases, infection of the central nervous system. Bp is a facultative intracellular pathogen and it can infect various cell types. The Bp intracellular lifecycle has been partially elucidated and is highly complex. Herein, we have identified a transcriptional regulator, BP1026B_II1198, that is differentially expressed as Bp transits through host cells. A deletion mutant of BP1026B_II1198 was attenuated in RAW264.7 cell and BALB/c mouse infection. To further characterize the function of this transcriptional regulator, we endeavored to determine the regulon of BP1026B_II1198. RNA-seq analysis showed the global picture of genes regulated while ChIP-seq analysis identified two specific BP1026B_II1198 binding regions on chromosome II. We investigated the transposon mutants of these genes controlled by BP1026B_II1198 and confirmed that these genes contribute to pathogenesis in RAW264.7 murine macrophage cells. Taken together, the data presented here shed light on the regulon of BP1026B_II1198 and its role during intracellular infection and highlights an integral portion of the highly complex regulation network of Bp during host infection.</p>', 'date' => '2021-05-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/34001967', 'doi' => '10.1038/s41598-021-89852-7', 'modified' => '2022-08-02 16:55:52', 'created' => '2022-05-19 10:41:50', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '4092', 'name' => 'Formation of the CenH3-Deficient Holocentromere in Lepidoptera AvoidsActive Chromatin.', 'authors' => 'Senaratne, Aruni P and Muller, Héloïse and Fryer, Kelsey A and Kawamoto,Munetaka and Katsuma, Susumu and Drinnenberg, Ines A', 'description' => '<p>Despite the essentiality for faithful chromosome segregation, centromere architectures are diverse among eukaryotes and embody two main configurations: mono- and holocentromeres, referring, respectively, to localized or unrestricted distribution of centromeric activity. Of the two, some holocentromeres offer the curious condition of having arisen independently in multiple insects, most of which have lost the otherwise essential centromere-specifying factor CenH3 (first described as CENP-A in humans). The loss of CenH3 raises intuitive questions about how holocentromeres are organized and regulated in CenH3-lacking insects. Here, we report the first chromatin-level description of CenH3-deficient holocentromeres by leveraging recently identified centromere components and genomics approaches to map and characterize the holocentromeres of the silk moth Bombyx mori, a representative lepidopteran insect lacking CenH3. This uncovered a robust correlation between the distribution of centromere sites and regions of low chromatin activity along B. mori chromosomes. Transcriptional perturbation experiments recapitulated the exclusion of B. mori centromeres from active chromatin. Based on reciprocal centromere occupancy patterns observed along differentially expressed orthologous genes of Lepidoptera, we further found that holocentromere formation in a manner that is recessive to chromatin dynamics is evolutionarily conserved. Our results help us discuss the plasticity of centromeres in the context of a role for the chromosome-wide chromatin landscape in conferring centromere identity rather than the presence of CenH3. Given the co-occurrence of CenH3 loss and holocentricity in insects, we further propose that the evolutionary establishment of holocentromeres in insects was facilitated through the loss of a CenH3-specified centromere.</p>', 'date' => '2020-10-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/33125865', 'doi' => '10.1016/j.cub.2020.09.078', 'modified' => '2021-03-17 17:13:50', 'created' => '2021-02-18 10:21:53', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3598', 'name' => 'Novel dual regulators of Pseudomonas aeruginosa essential for productive biofilms and virulence.', 'authors' => 'Heacock-Kang Y, Zarzycki-Siek J, Sun Z, Poonsuk K, Bluhm AP, Cabanas D, Fogen D, McMillan IA, Chuanchuen R, Hoang TT', 'description' => '<p>Gene regulation network in Pseudomonas aeruginosa is complex. With a relatively large genome (6.2 Mb), there is a significant portion of genes that are proven or predicted to be transcriptional regulators. Many of these regulators have been shown to play important roles in biofilm formation and maintenance. In this study, we present a novel transcriptional regulator, PA1226, which modulates biofilm formation and virulence in P. aeruginosa. Mutation in the gene encoding this regulator abolished the ability of P. aeruginosa to produce biofilms in vitro, without any effect on the planktonic growth. This regulator is also essential for the in vivo fitness and pathogenesis in both Drosophila melanogaster and BALB/c mouse lung infection models. Transcriptome analysis revealed that PA1226 regulates many essential virulence genes/pathways, including those involved in alginate, pili, and LPS biosynthesis. Genes/operons directly regulated by PA1226 and potential binding sequences were identified via ChIP-seq. Attempts to confirm the binding sequences by electrophoretic mobility shift assay led to the discovery of a co-regulator, PA1413, via co-immunoprecipitation assay. PA1226 and PA1413 were shown to bind collaboratively to the promoter regions of their regulons. A model is proposed, summarizing our finding on this novel dual-regulation system.</p>', 'date' => '2018-08-01', 'pmid' => 'http://www.pubmed.gov/29995308', 'doi' => '10.1111/mmi.14063', 'modified' => '2019-04-17 15:07:51', 'created' => '2019-04-16 12:25:30', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '334', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS GB en', 'language' => 'en', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-GB-en-GHS_1_0.pdf', 'countries' => 'GB', 'modified' => '2020-06-09 13:01:35', 'created' => '2020-06-09 13:01:35', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '336', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS US en', 'language' => 'en', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-US-en-GHS_1_0.pdf', 'countries' => 'US', 'modified' => '2020-06-09 13:03:27', 'created' => '2020-06-09 13:03:27', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '331', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS DE de', 'language' => 'de', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-DE-de-GHS_1_0.pdf', 'countries' => 'DE', 'modified' => '2020-06-09 12:59:50', 'created' => '2020-06-09 12:59:50', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '335', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-06-09 13:02:44', 'created' => '2020-06-09 13:02:44', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '330', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-BE-nl-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 12:59:19', 'created' => '2020-06-09 12:59:19', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '329', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-BE-fr-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 12:58:49', 'created' => '2020-06-09 12:58:49', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '333', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-FR-fr-GHS_1_0.pdf', 'countries' => 'FR', 'modified' => '2020-06-09 13:00:53', 'created' => '2020-06-09 13:00:53', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '332', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS ES es', 'language' => 'es', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 13:00:23', 'created' => '2020-06-09 13:00:23', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/diamag-anti-mouse-igg-coated-magnetic-beads-220-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '1910', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1.5 ml', 'catalog_number' => 'C03010022-150', 'old_catalog_number' => 'kch-821-150', 'sf_code' => 'C03010022-351', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '575', 'price_USD' => '405', 'price_GBP' => '520', 'price_JPY' => '90075', 'price_CNY' => '', 'price_AUD' => '1012', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-1-5-ml', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2016-07-19 10:45:27', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '178', 'product_id' => '1910', 'group_id' => '167' ) ), (int) 1 => array( 'id' => '1912', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '660 µl', 'catalog_number' => 'C03010022-660', 'old_catalog_number' => 'kch-821-660', 'sf_code' => 'C03010022-350', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '265', 'price_USD' => '250', 'price_GBP' => '245', 'price_JPY' => '41510', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-660-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:02', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '179', 'product_id' => '1912', 'group_id' => '167' ) ) ) $pro = array( 'id' => '1912', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '660 µl', 'catalog_number' => 'C03010022-660', 'old_catalog_number' => 'kch-821-660', 'sf_code' => 'C03010022-350', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '265', 'price_USD' => '250', 'price_GBP' => '245', 'price_JPY' => '41510', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-660-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:02', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '179', 'product_id' => '1912', 'group_id' => '167' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/en/p/auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns"><img src="/img/product/kits/methyl-kit-icon.png" alt="Methylation kit icon" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">C02010034</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1885" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/en/carts/add/1885" id="CartAdd/1885Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1885" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Auto hMeDIP kit x16 (monoclonal mouse antibody)</strong> to my shopping cart.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'C02010034', '690', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'C02010034', '690', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns" data-reveal-id="cartModal-1885" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Auto hMeDIP kit x16 (monoclonal mouse antibody)</h6> </div> </div> </li> ' $related = array( 'id' => '1885', 'antibody_id' => null, 'name' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'description' => '<p><span>The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation analysis. It features</span><span> a highly specific monoclonal antibody against </span><span>5-hydroxymethylcytosine (5-hmC) for the immunoprecipitation of hydroxymethylated DNA</span><span>. It includes control DNA and primers to assess the effiency of the assay. </span><span>Performing hydroxymethylation profiling with the hMeDIP kit is fast, reliable and highly specific.</span></p>', 'label1' => ' Characteristics', 'info1' => '<ul style="list-style-type: disc;"> <li><span>Robust enrichment & immunoprecipitation of hydroxymethylated DNA</span></li> <li>Highly specific monoclonal antibody against 5-hmC<span> for reliable, reproducible results</span></li> <li>Including control DNA and primers to <span>monitor the efficiency of the assay</span> <ul style="list-style-type: circle;"> <li>5-hmC, 5-mC and unmethylated DNA sequences and primer pairs</li> <li>Mouse primer pairs against Sfi1 targeting hydroxymethylated gene in mouse</li> </ul> </li> </ul> <ul style="list-style-type: disc;"> <li>Improved single-tube, magnetic bead-based protocol</li> </ul>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '16 rxns', 'catalog_number' => 'C02010034', 'old_catalog_number' => '', 'sf_code' => 'C02010034-', 'type' => 'RFR', 'search_order' => '04-undefined', 'price_EUR' => '630', 'price_USD' => '690', 'price_GBP' => '580', 'price_JPY' => '98690', 'price_CNY' => '', 'price_AUD' => '1725', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'auto-hmedip-kit-x16-monoclonal-mouse-antibody-16-rxns', 'meta_title' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'meta_keywords' => '', 'meta_description' => 'Auto hMeDIP kit x16 (monoclonal mouse antibody)', 'modified' => '2021-01-18 10:37:19', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( 'id' => '759', 'product_id' => '1911', 'related_id' => '1885' ), 'Image' => array( (int) 0 => array( 'id' => '1778', 'name' => 'product/kits/methyl-kit-icon.png', 'alt' => 'Methylation kit icon', 'modified' => '2019-04-23 15:17:01', 'created' => '2018-03-15 15:52:12', 'ProductsImage' => array( [maximum depth reached] ) ) ) ) $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(kch-821-660)</span>' $country_code = 'US' $other_format = array( 'id' => '1912', 'antibody_id' => null, 'name' => 'DiaMag anti-mouse IgG coated magnetic beads', 'description' => '<p><span>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP). The beads bind mouse IgG1, IgG2a, IgG2b. The beads should be washed before use.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '660 µl', 'catalog_number' => 'C03010022-660', 'old_catalog_number' => 'kch-821-660', 'sf_code' => 'C03010022-350', 'type' => 'REF', 'search_order' => '04-undefined', 'price_EUR' => '265', 'price_USD' => '250', 'price_GBP' => '245', 'price_JPY' => '41510', 'price_CNY' => '', 'price_AUD' => '625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-660-ul', 'meta_title' => 'DiaMag anti-mouse IgG coated magnetic beads', 'meta_keywords' => '', 'meta_description' => 'DiaMag anti-mouse IgG coated magnetic beads', 'modified' => '2020-05-27 13:52:02', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '179', 'product_id' => '1912', 'group_id' => '167' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '911', 'name' => 'DiaMag anti-mouse IgG-coated magnetic beads', 'description' => '<p>These magnetic beads coated with anti-mouse IgG antibody have been extensively validated in chromatin immunoprecipitation assay (ChIP) and methylated DNA immunoprecipitation assays (MeDIP).</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/c03010022-kch-821.pdf', 'slug' => 'diamag-anti-mouse-igg-coated-magnetic-beads-tds', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-07-19 10:45:17', 'created' => '2016-07-19 10:45:17', 'ProductsDocument' => array( 'id' => '2177', 'product_id' => '1911', 'document_id' => '911' ) ) $sds = array( 'id' => '332', 'name' => 'DiaMag anti-mouse IgG coated magnetic beads SDS ES es', 'language' => 'es', 'url' => 'files/SDS/DiaMag_anti-mouse/SDS-C03010022-DiaMag_anti-mouse_IgG_coated_magnetic_beads-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 13:00:23', 'created' => '2020-06-09 13:00:23', 'ProductsSafetySheet' => array( 'id' => '650', 'product_id' => '1911', 'safety_sheet_id' => '332' ) ) $publication = array( 'id' => '3598', 'name' => 'Novel dual regulators of Pseudomonas aeruginosa essential for productive biofilms and virulence.', 'authors' => 'Heacock-Kang Y, Zarzycki-Siek J, Sun Z, Poonsuk K, Bluhm AP, Cabanas D, Fogen D, McMillan IA, Chuanchuen R, Hoang TT', 'description' => '<p>Gene regulation network in Pseudomonas aeruginosa is complex. With a relatively large genome (6.2 Mb), there is a significant portion of genes that are proven or predicted to be transcriptional regulators. Many of these regulators have been shown to play important roles in biofilm formation and maintenance. In this study, we present a novel transcriptional regulator, PA1226, which modulates biofilm formation and virulence in P. aeruginosa. Mutation in the gene encoding this regulator abolished the ability of P. aeruginosa to produce biofilms in vitro, without any effect on the planktonic growth. This regulator is also essential for the in vivo fitness and pathogenesis in both Drosophila melanogaster and BALB/c mouse lung infection models. Transcriptome analysis revealed that PA1226 regulates many essential virulence genes/pathways, including those involved in alginate, pili, and LPS biosynthesis. Genes/operons directly regulated by PA1226 and potential binding sequences were identified via ChIP-seq. Attempts to confirm the binding sequences by electrophoretic mobility shift assay led to the discovery of a co-regulator, PA1413, via co-immunoprecipitation assay. PA1226 and PA1413 were shown to bind collaboratively to the promoter regions of their regulons. A model is proposed, summarizing our finding on this novel dual-regulation system.</p>', 'date' => '2018-08-01', 'pmid' => 'http://www.pubmed.gov/29995308', 'doi' => '10.1111/mmi.14063', 'modified' => '2019-04-17 15:07:51', 'created' => '2019-04-16 12:25:30', 'ProductsPublication' => array( 'id' => '3470', 'product_id' => '1911', 'publication_id' => '3598' ) ) $externalLink = ' <a href="http://www.pubmed.gov/29995308" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
To ensure you see the information most relevant to you, please select your country. Please note that your browser will need to be configured to accept cookies.
Diagenode will process your personal data in strict accordance with its privacy policy. This will include sending you updates about us, our products, and resources we think would be of interest to you.
