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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on total RNA isolated from 6 million SK-N-SH cells using 15 µg of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) or with an equal amount of rabbit IgG, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the ELAVL3 antibody (upper right). The lower figure shows the gel image for the negative IgG control, the ELAVL3 antibody, and the input (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 µg of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 µg of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode antibody directed against ELAVL3</strong><br />Whole cell extracts from SK-N-SH cells (lane 1) and from P19 cells treated with retinoic acid (lane 2) were analysed by Western blot using the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348) diluted 1:1,000 in PBS containing 1% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong>Figure 3. Immunoprecipitation using the Diagenode antibody directed against ELAVL3</strong><br />Immunoprecipitation was performed on whole cell extracts from SK-N-SH using 5 µg of the Diagenode antibody against ELAVL3 (<span>Cat</span>. No. C15410348, lane 2). An equal amount of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated ELAVL3 protein was subsequently detected by western blot with the ELAVL3 antibody as described above.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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'hide' => true,
'all_format' => false,
'is_antibody' => true,
'slug' => 'mirna-sirna',
'cookies_tag_id' => null,
'meta_keywords' => 'miRNA & siRNA meta Polyclonal antibody,Monoclonal antibody',
'meta_description' => 'Diagenode offers Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing',
'meta_title' => 'Polyclonal and Monoclonal antibody used for miRNA & siRNA silencing | Diagenode',
'modified' => '2020-09-01 11:42:06',
'created' => '2014-11-12 02:34:56',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '1019',
'name' => 'ELAVL3 polyclonal antibody datasheet',
'description' => '<p>Polyclonal antibody raised in rabbit against human ELAVL3 (ELAV Like RNA Binding Protein 3), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => '../files/products/antibodies/elavl3-polyclonal-antibody-100-ul.pdf',
'slug' => 'elavl3-polyclonal-antibody-100-ul-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-07-11 15:58:42',
'created' => '2018-07-11 15:58:42',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(
(int) 0 => array(
'id' => '250',
'name' => 'product/antibodies/antibody.png',
'alt' => 'Mouse IgG',
'modified' => '2020-11-27 07:00:09',
'created' => '2015-07-17 10:12:18',
'ProductsImage' => array(
[maximum depth reached]
)
)
),
'Promotion' => array(),
'Protocol' => array(),
'Publication' => array(),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array()
)
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '30',
'position' => '10',
'parent_id' => '40',
'name' => 'IP',
'description' => '<p>Immunoprecipitation</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunoprecipitation',
'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:23:07',
'created' => '2015-07-08 13:46:50',
'ProductsApplication' => array(
'id' => '4915',
'product_id' => '2975',
'application_id' => '30'
)
)
$slugs = array(
(int) 0 => 'immunoprecipitation'
)
$applications = array(
'id' => '30',
'position' => '10',
'parent_id' => '40',
'name' => 'IP',
'description' => '<p>Immunoprecipitation</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunoprecipitation',
'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:23:07',
'created' => '2015-07-08 13:46:50',
'locale' => 'eng'
)
$description = '<p>Immunoprecipitation</p>'
$name = 'IP'
$document = array(
'id' => '1019',
'name' => 'ELAVL3 polyclonal antibody datasheet',
'description' => '<p>Polyclonal antibody raised in rabbit against human ELAVL3 (ELAV Like RNA Binding Protein 3), using a KLH-conjugated synthetic peptide from the N-teminal part of the protein.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => '../files/products/antibodies/elavl3-polyclonal-antibody-100-ul.pdf',
'slug' => 'elavl3-polyclonal-antibody-100-ul-tds',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-07-11 15:58:42',
'created' => '2018-07-11 15:58:42',
'ProductsDocument' => array(
'id' => '2658',
'product_id' => '2975',
'document_id' => '1019'
)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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