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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP assays were performed using SKNO-1 cells, the Diagenode antibody against ETO (Cat. No. C15310001) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used. </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP was performed as described above. The IP’d DNA of 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow. </small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ETO (Cat. No. C15310001). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,300. </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP assays were performed using SKNO-1 cells, the Diagenode antibody against ETO (Cat. No. C15310001) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used. </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP was performed as described above. The IP’d DNA of 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow. </small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ETO (Cat. No. C15310001). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,300. </small></p>
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<p>Polyclonal antibody raised in rabbit against human<strong> ETO (runt-related transcription factor 1; translocated to, 1 (cyclin D-related))</strong> using two KLH-conjugated synthetic peptides containing sequences from the N-terminal and the central region of the protein, respectively.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP assays were performed using SKNO-1 cells, the Diagenode antibody against ETO (Cat. No. C15310001) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used. </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP was performed as described above. The IP’d DNA of 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow. </small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ETO (Cat. No. C15310001). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,300. </small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/22983443',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP assays were performed using SKNO-1 cells, the Diagenode antibody against ETO (Cat. No. C15310001) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used. </small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ETO (Cat. No. C15310001). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,300. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIPseq-C.png" alt="ETO Antibody for ChIP-seq assay" caption="false" width="354" height="68" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIPseq-D.png" alt="ETO Antibody validated in ChIP-seq " caption="false" width="354" height="70" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP was performed as described above. The IP’d DNA of 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ELISA.png" alt="ETO Antibody ELISA Validation" caption="false" width="278" height="210" /></p>
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<p><small><strong> Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ETO (Cat. No. C15310001). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,300. </small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p>Polyclonal antibody raised in rabbit against human<strong> ETO (runt-related transcription factor 1; translocated to, 1 (cyclin D-related))</strong> using two KLH-conjugated synthetic peptides containing sequences from the N-terminal and the central region of the protein, respectively.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP assays were performed using SKNO-1 cells, the Diagenode antibody against ETO (Cat. No. C15310001) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used. </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP was performed as described above. The IP’d DNA of 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow. </small></p>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 μl/ChIP</td>
<td>Fig 1, 2</td>
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<tr>
<td>ELISA</td>
<td>1:100</td>
<td>Fig 3</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 μl per IP.</small></p>',
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'description' => '<p>Alternative names: <strong>RUNX1T1</strong>, <strong>AML1T1</strong>, <strong>CBFA2T1</strong>, <strong>CDR</strong>, <strong>MTG8</strong>, <strong>ZMYND2</strong></p>
<p>Polyclonal antibody raised in rabbit against human<strong> ETO (runt-related transcription factor 1; translocated to, 1 (cyclin D-related))</strong> using two KLH-conjugated synthetic peptides containing sequences from the N-terminal and the central region of the protein, respectively.</p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIP.png" alt="ETO Antibody ChIP Grade" caption="false" width="278" height="211" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP assays were performed using SKNO-1 cells, the Diagenode antibody against ETO (Cat. No. C15310001) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIPseq-A.png" alt="ETO Antibody ChIP-seq Grade" caption="false" width="354" height="50" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIPseq-B.png" alt="ETO Antibody for ChIP-seq" caption="false" width="354" height="97" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIPseq-C.png" alt="ETO Antibody for ChIP-seq assay" caption="false" width="354" height="68" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIPseq-D.png" alt="ETO Antibody validated in ChIP-seq " caption="false" width="354" height="70" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP was performed as described above. The IP’d DNA of 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ELISA.png" alt="ETO Antibody ELISA Validation" caption="false" width="278" height="210" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ETO (Cat. No. C15310001). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,300. </small></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p>Polyclonal antibody raised in rabbit against human<strong> ETO (runt-related transcription factor 1; translocated to, 1 (cyclin D-related))</strong> using two KLH-conjugated synthetic peptides containing sequences from the N-terminal and the central region of the protein, respectively.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP assays were performed using SKNO-1 cells, the Diagenode antibody against ETO (Cat. No. C15310001) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used. </small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP was performed as described above. The IP’d DNA of 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow. </small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ETO (Cat. No. C15310001). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,300. </small></p>
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'description' => '<p>Alternative names: <strong>RUNX1T1</strong>, <strong>AML1T1</strong>, <strong>CBFA2T1</strong>, <strong>CDR</strong>, <strong>MTG8</strong>, <strong>ZMYND2</strong></p>
<p>Polyclonal antibody raised in rabbit against human<strong> ETO (runt-related transcription factor 1; translocated to, 1 (cyclin D-related))</strong> using two KLH-conjugated synthetic peptides containing sequences from the N-terminal and the central region of the protein, respectively.</p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIP.png" alt="ETO Antibody ChIP Grade" caption="false" width="278" height="211" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP assays were performed using SKNO-1 cells, the Diagenode antibody against ETO (Cat. No. C15310001) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used. </small></p>
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<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIPseq-A.png" alt="ETO Antibody ChIP-seq Grade" caption="false" width="354" height="50" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIPseq-B.png" alt="ETO Antibody for ChIP-seq" caption="false" width="354" height="97" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIPseq-C.png" alt="ETO Antibody for ChIP-seq assay" caption="false" width="354" height="68" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIPseq-D.png" alt="ETO Antibody validated in ChIP-seq " caption="false" width="354" height="70" /></p>
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<div class="small-7 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP was performed as described above. The IP’d DNA of 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ELISA.png" alt="ETO Antibody ELISA Validation" caption="false" width="278" height="210" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ETO (Cat. No. C15310001). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,300. </small></p>
</div>
</div>',
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'application_table' => '<table>
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<th>References</th>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>4 μl/ChIP</td>
<td>Fig 1, 2</td>
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<tr>
<td>ELISA</td>
<td>1:100</td>
<td>Fig 3</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-10 μl per IP.</small></p>',
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'description' => '<p>Alternative names: <strong>RUNX1T1</strong>, <strong>AML1T1</strong>, <strong>CBFA2T1</strong>, <strong>CDR</strong>, <strong>MTG8</strong>, <strong>ZMYND2</strong></p>
<p>Polyclonal antibody raised in rabbit against human<strong> ETO (runt-related transcription factor 1; translocated to, 1 (cyclin D-related))</strong> using two KLH-conjugated synthetic peptides containing sequences from the N-terminal and the central region of the protein, respectively.</p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIP.png" alt="ETO Antibody ChIP Grade" caption="false" width="278" height="211" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP assays were performed using SKNO-1 cells, the Diagenode antibody against ETO (Cat. No. C15310001) and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Figure 1 shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used. </small></p>
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<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIPseq-A.png" alt="ETO Antibody ChIP-seq Grade" caption="false" width="354" height="50" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIPseq-B.png" alt="ETO Antibody for ChIP-seq" caption="false" width="354" height="97" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIPseq-C.png" alt="ETO Antibody for ChIP-seq assay" caption="false" width="354" height="68" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ChIPseq-D.png" alt="ETO Antibody validated in ChIP-seq " caption="false" width="354" height="70" /></p>
</div>
<div class="small-7 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against ETO </strong><br />ChIP was performed as described above. The IP’d DNA of 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure 2 shows the results of the complete chromosome 3 and three genomic regions surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow. </small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15310001_ELISA.png" alt="ETO Antibody ELISA Validation" caption="false" width="278" height="210" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 3. Determination of the antibody titer </strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human ETO (Cat. No. C15310001). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:1,300. </small></p>
</div>
</div>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<li><span style="font-weight: 400;"> Highly sensitive and specific</span></li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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<p></p>
<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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