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Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein.
| Lot | A280-004 |
|---|---|
| Concentration | not determined |
| Species reactivity | |
| Type | Polyclonal |
| Purity | Whole antiserum from rabbit containing 0.05% azide. |
| Host | Rabbit |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:500 | Fig 1 |
| Western Blotting | 1:500 | Fig 2, (1) |
Figure 1. Determination of the antibody titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. CS-169-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against Flp
Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. CS-169-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ELISA Enzyme-linked immunosorbent assay. Read more |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
| Datasheet Flp C15310169 DATASHEET Datasheet description | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: Flp Antibody (Sample Size) (Diagenode Cat# C15310169-20 Lot# A280-004). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Development of a lentivirus vector-based assay for non-destructive monitoring of cell fusion activity. |
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Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. CS-169-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp</strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. CS-169-100), diluted 1:500 in BSA/PBS-Tween. 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Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. C15310169) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp <sup>(1)</sup></strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. C15310169), diluted 1:500 in BSA/PBS-Tween. 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Classified based on level of validation for flexibility of application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode offers Antibodies exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-04 16:02:57', 'created' => '2014-12-25 12:45:50', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. 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One way to accomplish this goal is by using a bipartite lentivirus vector (LV)-based cell fusion assay system in which the cellular fusion partners are transduced with a flippase-activatable Photinus pyralis luciferase (PpLuc) expression unit (acceptor cells) or with a recombinant gene encoding FLPeNLS+, a nuclear-targeted and molecularly evolved version of flippase (donor cells). Fusion of both cell populations will lead to the FLPe-dependent generation of a functional PpLuc gene. PpLuc activity is typically measured in cell lysates, precluding consecutive analysis of one cell culture. Therefore, in this study the PpLuc-coding sequence was replaced by that of Gaussia princeps luciferase (GpLuc), a secretory protein allowing repeated analysis of the same cell culture. In myotubes the spread of FLPeNLS+ may be limited due to its nuclear localization signal (NLS) causing low signal outputs. To test this hypothesis, myoblasts were transduced with LVs encoding either FLPeNLS+ or an NLS-less version of FLPe (FLPeNLS-) and subsequently co-cultured in different ratios with myoblasts containing the FLPe-activatable GpLuc expression cassette. At different times after induction of cell-to-cell fusion the GpLuc activity in the culture medium was determined. FLPeNLS+ and FLPeNLS- both activated the latent GpLuc gene but when the percentage of FLPe-expressing myoblasts was limiting, FLPeNLS+ generally yielded slightly higher signals than FLPeNLS- while at low acceptor-to-donor cell ratios FLPeNLS- was usually superior. The ability of FLPeNLS+ to spread through myofibers and to induce reporter gene expression is thus not limited by its NLS. However, at high FLPe concentrations the presence of the NLS negatively affected reporter gene expression. 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Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. C15310169) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp <sup>(1)</sup></strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. C15310169), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310169', 'old_catalog_number' => 'CS-169-100', 'sf_code' => 'C15310169-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 12, 2021', 'slug' => 'flp-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'Flp Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Flp (Flp recombinase) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website.', 'modified' => '2021-03-05 14:23:00', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '3036', 'antibody_id' => '325', 'name' => 'Flp Antibody (Sample Size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. CS-169-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp</strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. CS-169-100), diluted 1:500 in BSA/PBS-Tween. 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Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. C15310169) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp <sup>(1)</sup></strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. C15310169), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310169', 'old_catalog_number' => 'CS-169-100', 'sf_code' => 'C15310169-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 12, 2021', 'slug' => 'flp-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'Flp Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Flp (Flp recombinase) Polyclonal Antibody validated in WB and ELISA. 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One way to accomplish this goal is by using a bipartite lentivirus vector (LV)-based cell fusion assay system in which the cellular fusion partners are transduced with a flippase-activatable Photinus pyralis luciferase (PpLuc) expression unit (acceptor cells) or with a recombinant gene encoding FLPeNLS+, a nuclear-targeted and molecularly evolved version of flippase (donor cells). Fusion of both cell populations will lead to the FLPe-dependent generation of a functional PpLuc gene. PpLuc activity is typically measured in cell lysates, precluding consecutive analysis of one cell culture. Therefore, in this study the PpLuc-coding sequence was replaced by that of Gaussia princeps luciferase (GpLuc), a secretory protein allowing repeated analysis of the same cell culture. In myotubes the spread of FLPeNLS+ may be limited due to its nuclear localization signal (NLS) causing low signal outputs. To test this hypothesis, myoblasts were transduced with LVs encoding either FLPeNLS+ or an NLS-less version of FLPe (FLPeNLS-) and subsequently co-cultured in different ratios with myoblasts containing the FLPe-activatable GpLuc expression cassette. At different times after induction of cell-to-cell fusion the GpLuc activity in the culture medium was determined. FLPeNLS+ and FLPeNLS- both activated the latent GpLuc gene but when the percentage of FLPe-expressing myoblasts was limiting, FLPeNLS+ generally yielded slightly higher signals than FLPeNLS- while at low acceptor-to-donor cell ratios FLPeNLS- was usually superior. The ability of FLPeNLS+ to spread through myofibers and to induce reporter gene expression is thus not limited by its NLS. However, at high FLPe concentrations the presence of the NLS negatively affected reporter gene expression. In summary, a rapid and simple chemiluminescence assay for quantifying cell-to-cell fusion progression based on GpLuc has been developed.</p>', 'date' => '2014-07-16', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25028973', 'doi' => '', 'modified' => '2016-04-11 10:35:35', 'created' => '2015-07-24 15:39:03', 'ProductsPublication' => array( 'id' => '4016', 'product_id' => '3036', 'publication_id' => '2181' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/25028973" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'meta_title' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'product' => array( 'Product' => array( 'id' => '3036', 'antibody_id' => '325', 'name' => 'Flp Antibody (Sample Size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. CS-169-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp</strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. CS-169-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15310169-20', 'old_catalog_number' => '', 'sf_code' => 'C15310169-361', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '/', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'flp-polyclonal-antibody-pioneer-20-ul-sample-size', 'meta_title' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'meta_keywords' => '', 'meta_description' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'modified' => '2021-12-20 14:10:44', 'created' => '2019-08-13 10:39:53', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '325', 'name' => 'Flp polyclonal antibody', 'description' => 'Flp recombinase originates from Saccharomyces cerevisiae and recognizes FRT sites in the genome. It catalyzes recombination between two FRT sites, thereby excising the intervening DNA sequence. Flp recombinase is used as a tool for the generation of transgenic animals.', 'clonality' => '', 'isotype' => '', 'lot' => 'A280-004', 'concentration' => 'not determined', 'reactivity' => '', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum from rabbit containing 0.05% azide.', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:500</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:500</td> <td>Fig 2, (1)</td> </tr> </tbody> </table> <p></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-03-11 15:23:02', 'created' => '0000-00-00 00:00:00', 'select_label' => '325 - Flp polyclonal antibody (A280-004 - not determined - - Whole antiserum from rabbit containing 0.05% azide. - Rabbit)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/flp-polyclonal-antibody-pioneer-100-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'Flp polyclonal antibody - Pioneer (Sample Size)' $meta_title = 'Flp polyclonal antibody - Pioneer (Sample Size)' $product = array( 'Product' => array( 'id' => '3036', 'antibody_id' => '325', 'name' => 'Flp Antibody (Sample Size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. CS-169-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp</strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. CS-169-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15310169-20', 'old_catalog_number' => '', 'sf_code' => 'C15310169-361', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '/', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'flp-polyclonal-antibody-pioneer-20-ul-sample-size', 'meta_title' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'meta_keywords' => '', 'meta_description' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'modified' => '2021-12-20 14:10:44', 'created' => '2019-08-13 10:39:53', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '325', 'name' => 'Flp polyclonal antibody', 'description' => 'Flp recombinase originates from Saccharomyces cerevisiae and recognizes FRT sites in the genome. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-03-11 15:23:02', 'created' => '0000-00-00 00:00:00', 'select_label' => '325 - Flp polyclonal antibody (A280-004 - not determined - - Whole antiserum from rabbit containing 0.05% azide. - Rabbit)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '267', 'name' => 'C15310169', 'product_id' => '2133', 'modified' => '2019-08-13 10:39:28', 'created' => '2019-08-13 10:39:28' ), 'Master' => array( 'id' => '2133', 'antibody_id' => '325', 'name' => 'Flp Antibody ', 'description' => '<p>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein <sup>(1)</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. C15310169) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp <sup>(1)</sup></strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. C15310169), diluted 1:500 in BSA/PBS-Tween. 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Classified based on level of validation for flexibility of application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode offers Antibodies exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-04 16:02:57', 'created' => '2014-12-25 12:45:50', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. 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Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '429', 'name' => 'Datasheet Flp C15310169', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_Flp_CS-169-100.pdf', 'slug' => 'datasheet-flp-cs-169-100', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-03-05 14:25:51', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2181', 'name' => 'Development of a lentivirus vector-based assay for non-destructive monitoring of cell fusion activity.', 'authors' => 'Neshati Z, Liu J, Zhou G, Schalij MJ, de Vries AA', 'description' => '<p>Cell-to-cell fusion can be quantified by endowing acceptor and donor cells with latent reporter genes/proteins and activators of these genes/proteins, respectively. One way to accomplish this goal is by using a bipartite lentivirus vector (LV)-based cell fusion assay system in which the cellular fusion partners are transduced with a flippase-activatable Photinus pyralis luciferase (PpLuc) expression unit (acceptor cells) or with a recombinant gene encoding FLPeNLS+, a nuclear-targeted and molecularly evolved version of flippase (donor cells). Fusion of both cell populations will lead to the FLPe-dependent generation of a functional PpLuc gene. PpLuc activity is typically measured in cell lysates, precluding consecutive analysis of one cell culture. Therefore, in this study the PpLuc-coding sequence was replaced by that of Gaussia princeps luciferase (GpLuc), a secretory protein allowing repeated analysis of the same cell culture. In myotubes the spread of FLPeNLS+ may be limited due to its nuclear localization signal (NLS) causing low signal outputs. To test this hypothesis, myoblasts were transduced with LVs encoding either FLPeNLS+ or an NLS-less version of FLPe (FLPeNLS-) and subsequently co-cultured in different ratios with myoblasts containing the FLPe-activatable GpLuc expression cassette. At different times after induction of cell-to-cell fusion the GpLuc activity in the culture medium was determined. FLPeNLS+ and FLPeNLS- both activated the latent GpLuc gene but when the percentage of FLPe-expressing myoblasts was limiting, FLPeNLS+ generally yielded slightly higher signals than FLPeNLS- while at low acceptor-to-donor cell ratios FLPeNLS- was usually superior. The ability of FLPeNLS+ to spread through myofibers and to induce reporter gene expression is thus not limited by its NLS. However, at high FLPe concentrations the presence of the NLS negatively affected reporter gene expression. In summary, a rapid and simple chemiluminescence assay for quantifying cell-to-cell fusion progression based on GpLuc has been developed.</p>', 'date' => '2014-07-16', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25028973', 'doi' => '', 'modified' => '2016-04-11 10:35:35', 'created' => '2015-07-24 15:39:03', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '256', 'name' => 'Flp antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2020-06-08 17:19:02', 'created' => '2020-06-08 17:19:02', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '254', 'name' => 'Flp antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2020-06-08 17:13:38', 'created' => '2020-06-08 17:13:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '249', 'name' => 'Flp antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-08 17:09:23', 'created' => '2020-06-08 17:09:23', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '253', 'name' => 'Flp antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2020-06-08 17:13:04', 'created' => '2020-06-08 17:13:04', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '252', 'name' => 'Flp antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-08 17:11:45', 'created' => '2020-06-08 17:11:45', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '251', 'name' => 'Flp antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2020-06-08 17:11:16', 'created' => '2020-06-08 17:11:16', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '255', 'name' => 'Flp antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-06-08 17:18:31', 'created' => '2020-06-08 17:18:31', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '250', 'name' => 'Flp antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-08 17:10:18', 'created' => '2020-06-08 17:10:18', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/flp-polyclonal-antibody-pioneer-100-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2133', 'antibody_id' => '325', 'name' => 'Flp Antibody ', 'description' => '<p>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein <sup>(1)</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. C15310169) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp <sup>(1)</sup></strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. C15310169), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310169', 'old_catalog_number' => 'CS-169-100', 'sf_code' => 'C15310169-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 12, 2021', 'slug' => 'flp-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'Flp Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Flp (Flp recombinase) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website.', 'modified' => '2021-03-05 14:23:00', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '3036', 'antibody_id' => '325', 'name' => 'Flp Antibody (Sample Size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. CS-169-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp</strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. CS-169-100), diluted 1:500 in BSA/PBS-Tween. 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Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. C15310169) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp <sup>(1)</sup></strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. C15310169), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310169', 'old_catalog_number' => 'CS-169-100', 'sf_code' => 'C15310169-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 12, 2021', 'slug' => 'flp-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'Flp Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Flp (Flp recombinase) Polyclonal Antibody validated in WB and ELISA. 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One way to accomplish this goal is by using a bipartite lentivirus vector (LV)-based cell fusion assay system in which the cellular fusion partners are transduced with a flippase-activatable Photinus pyralis luciferase (PpLuc) expression unit (acceptor cells) or with a recombinant gene encoding FLPeNLS+, a nuclear-targeted and molecularly evolved version of flippase (donor cells). Fusion of both cell populations will lead to the FLPe-dependent generation of a functional PpLuc gene. PpLuc activity is typically measured in cell lysates, precluding consecutive analysis of one cell culture. Therefore, in this study the PpLuc-coding sequence was replaced by that of Gaussia princeps luciferase (GpLuc), a secretory protein allowing repeated analysis of the same cell culture. In myotubes the spread of FLPeNLS+ may be limited due to its nuclear localization signal (NLS) causing low signal outputs. To test this hypothesis, myoblasts were transduced with LVs encoding either FLPeNLS+ or an NLS-less version of FLPe (FLPeNLS-) and subsequently co-cultured in different ratios with myoblasts containing the FLPe-activatable GpLuc expression cassette. At different times after induction of cell-to-cell fusion the GpLuc activity in the culture medium was determined. FLPeNLS+ and FLPeNLS- both activated the latent GpLuc gene but when the percentage of FLPe-expressing myoblasts was limiting, FLPeNLS+ generally yielded slightly higher signals than FLPeNLS- while at low acceptor-to-donor cell ratios FLPeNLS- was usually superior. The ability of FLPeNLS+ to spread through myofibers and to induce reporter gene expression is thus not limited by its NLS. However, at high FLPe concentrations the presence of the NLS negatively affected reporter gene expression. In summary, a rapid and simple chemiluminescence assay for quantifying cell-to-cell fusion progression based on GpLuc has been developed.</p>', 'date' => '2014-07-16', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25028973', 'doi' => '', 'modified' => '2016-04-11 10:35:35', 'created' => '2015-07-24 15:39:03', 'ProductsPublication' => array( 'id' => '4016', 'product_id' => '3036', 'publication_id' => '2181' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/25028973" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'meta_title' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'product' => array( 'Product' => array( 'id' => '3036', 'antibody_id' => '325', 'name' => 'Flp Antibody (Sample Size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. CS-169-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp</strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. CS-169-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15310169-20', 'old_catalog_number' => '', 'sf_code' => 'C15310169-361', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '/', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'flp-polyclonal-antibody-pioneer-20-ul-sample-size', 'meta_title' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'meta_keywords' => '', 'meta_description' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'modified' => '2021-12-20 14:10:44', 'created' => '2019-08-13 10:39:53', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '325', 'name' => 'Flp polyclonal antibody', 'description' => 'Flp recombinase originates from Saccharomyces cerevisiae and recognizes FRT sites in the genome. It catalyzes recombination between two FRT sites, thereby excising the intervening DNA sequence. Flp recombinase is used as a tool for the generation of transgenic animals.', 'clonality' => '', 'isotype' => '', 'lot' => 'A280-004', 'concentration' => 'not determined', 'reactivity' => '', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum from rabbit containing 0.05% azide.', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:500</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:500</td> <td>Fig 2, (1)</td> </tr> </tbody> </table> <p></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-03-11 15:23:02', 'created' => '0000-00-00 00:00:00', 'select_label' => '325 - Flp polyclonal antibody (A280-004 - not determined - - Whole antiserum from rabbit containing 0.05% azide. - Rabbit)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/flp-polyclonal-antibody-pioneer-100-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'Flp polyclonal antibody - Pioneer (Sample Size)' $meta_title = 'Flp polyclonal antibody - Pioneer (Sample Size)' $product = array( 'Product' => array( 'id' => '3036', 'antibody_id' => '325', 'name' => 'Flp Antibody (Sample Size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. CS-169-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp</strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. CS-169-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15310169-20', 'old_catalog_number' => '', 'sf_code' => 'C15310169-361', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '/', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'flp-polyclonal-antibody-pioneer-20-ul-sample-size', 'meta_title' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'meta_keywords' => '', 'meta_description' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'modified' => '2021-12-20 14:10:44', 'created' => '2019-08-13 10:39:53', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '325', 'name' => 'Flp polyclonal antibody', 'description' => 'Flp recombinase originates from Saccharomyces cerevisiae and recognizes FRT sites in the genome. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-03-11 15:23:02', 'created' => '0000-00-00 00:00:00', 'select_label' => '325 - Flp polyclonal antibody (A280-004 - not determined - - Whole antiserum from rabbit containing 0.05% azide. - Rabbit)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '267', 'name' => 'C15310169', 'product_id' => '2133', 'modified' => '2019-08-13 10:39:28', 'created' => '2019-08-13 10:39:28' ), 'Master' => array( 'id' => '2133', 'antibody_id' => '325', 'name' => 'Flp Antibody ', 'description' => '<p>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein <sup>(1)</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. C15310169) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp <sup>(1)</sup></strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. C15310169), diluted 1:500 in BSA/PBS-Tween. 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Classified based on level of validation for flexibility of application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode offers Antibodies exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-04 16:02:57', 'created' => '2014-12-25 12:45:50', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. 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Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '429', 'name' => 'Datasheet Flp C15310169', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_Flp_CS-169-100.pdf', 'slug' => 'datasheet-flp-cs-169-100', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-03-05 14:25:51', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2181', 'name' => 'Development of a lentivirus vector-based assay for non-destructive monitoring of cell fusion activity.', 'authors' => 'Neshati Z, Liu J, Zhou G, Schalij MJ, de Vries AA', 'description' => '<p>Cell-to-cell fusion can be quantified by endowing acceptor and donor cells with latent reporter genes/proteins and activators of these genes/proteins, respectively. One way to accomplish this goal is by using a bipartite lentivirus vector (LV)-based cell fusion assay system in which the cellular fusion partners are transduced with a flippase-activatable Photinus pyralis luciferase (PpLuc) expression unit (acceptor cells) or with a recombinant gene encoding FLPeNLS+, a nuclear-targeted and molecularly evolved version of flippase (donor cells). Fusion of both cell populations will lead to the FLPe-dependent generation of a functional PpLuc gene. PpLuc activity is typically measured in cell lysates, precluding consecutive analysis of one cell culture. Therefore, in this study the PpLuc-coding sequence was replaced by that of Gaussia princeps luciferase (GpLuc), a secretory protein allowing repeated analysis of the same cell culture. In myotubes the spread of FLPeNLS+ may be limited due to its nuclear localization signal (NLS) causing low signal outputs. To test this hypothesis, myoblasts were transduced with LVs encoding either FLPeNLS+ or an NLS-less version of FLPe (FLPeNLS-) and subsequently co-cultured in different ratios with myoblasts containing the FLPe-activatable GpLuc expression cassette. At different times after induction of cell-to-cell fusion the GpLuc activity in the culture medium was determined. FLPeNLS+ and FLPeNLS- both activated the latent GpLuc gene but when the percentage of FLPe-expressing myoblasts was limiting, FLPeNLS+ generally yielded slightly higher signals than FLPeNLS- while at low acceptor-to-donor cell ratios FLPeNLS- was usually superior. The ability of FLPeNLS+ to spread through myofibers and to induce reporter gene expression is thus not limited by its NLS. However, at high FLPe concentrations the presence of the NLS negatively affected reporter gene expression. 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Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. C15310169) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp <sup>(1)</sup></strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. C15310169), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310169', 'old_catalog_number' => 'CS-169-100', 'sf_code' => 'C15310169-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 12, 2021', 'slug' => 'flp-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'Flp Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Flp (Flp recombinase) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website.', 'modified' => '2021-03-05 14:23:00', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '3036', 'antibody_id' => '325', 'name' => 'Flp Antibody (Sample Size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. CS-169-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp</strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. CS-169-100), diluted 1:500 in BSA/PBS-Tween. 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Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. C15310169) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp <sup>(1)</sup></strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. C15310169), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310169', 'old_catalog_number' => 'CS-169-100', 'sf_code' => 'C15310169-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 12, 2021', 'slug' => 'flp-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'Flp Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Flp (Flp recombinase) Polyclonal Antibody validated in WB and ELISA. 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One way to accomplish this goal is by using a bipartite lentivirus vector (LV)-based cell fusion assay system in which the cellular fusion partners are transduced with a flippase-activatable Photinus pyralis luciferase (PpLuc) expression unit (acceptor cells) or with a recombinant gene encoding FLPeNLS+, a nuclear-targeted and molecularly evolved version of flippase (donor cells). Fusion of both cell populations will lead to the FLPe-dependent generation of a functional PpLuc gene. PpLuc activity is typically measured in cell lysates, precluding consecutive analysis of one cell culture. Therefore, in this study the PpLuc-coding sequence was replaced by that of Gaussia princeps luciferase (GpLuc), a secretory protein allowing repeated analysis of the same cell culture. In myotubes the spread of FLPeNLS+ may be limited due to its nuclear localization signal (NLS) causing low signal outputs. To test this hypothesis, myoblasts were transduced with LVs encoding either FLPeNLS+ or an NLS-less version of FLPe (FLPeNLS-) and subsequently co-cultured in different ratios with myoblasts containing the FLPe-activatable GpLuc expression cassette. At different times after induction of cell-to-cell fusion the GpLuc activity in the culture medium was determined. FLPeNLS+ and FLPeNLS- both activated the latent GpLuc gene but when the percentage of FLPe-expressing myoblasts was limiting, FLPeNLS+ generally yielded slightly higher signals than FLPeNLS- while at low acceptor-to-donor cell ratios FLPeNLS- was usually superior. The ability of FLPeNLS+ to spread through myofibers and to induce reporter gene expression is thus not limited by its NLS. However, at high FLPe concentrations the presence of the NLS negatively affected reporter gene expression. In summary, a rapid and simple chemiluminescence assay for quantifying cell-to-cell fusion progression based on GpLuc has been developed.</p>', 'date' => '2014-07-16', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25028973', 'doi' => '', 'modified' => '2016-04-11 10:35:35', 'created' => '2015-07-24 15:39:03', 'ProductsPublication' => array( 'id' => '4016', 'product_id' => '3036', 'publication_id' => '2181' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/25028973" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'meta_title' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'product' => array( 'Product' => array( 'id' => '3036', 'antibody_id' => '325', 'name' => 'Flp Antibody (Sample Size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. CS-169-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp</strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. CS-169-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15310169-20', 'old_catalog_number' => '', 'sf_code' => 'C15310169-361', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '/', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'flp-polyclonal-antibody-pioneer-20-ul-sample-size', 'meta_title' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'meta_keywords' => '', 'meta_description' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'modified' => '2021-12-20 14:10:44', 'created' => '2019-08-13 10:39:53', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '325', 'name' => 'Flp polyclonal antibody', 'description' => 'Flp recombinase originates from Saccharomyces cerevisiae and recognizes FRT sites in the genome. It catalyzes recombination between two FRT sites, thereby excising the intervening DNA sequence. Flp recombinase is used as a tool for the generation of transgenic animals.', 'clonality' => '', 'isotype' => '', 'lot' => 'A280-004', 'concentration' => 'not determined', 'reactivity' => '', 'type' => 'Polyclonal', 'purity' => 'Whole antiserum from rabbit containing 0.05% azide.', 'classification' => 'Pioneer', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ELISA</td> <td>1:500</td> <td>Fig 1</td> </tr> <tr> <td>Western Blotting</td> <td>1:500</td> <td>Fig 2, (1)</td> </tr> </tbody> </table> <p></p>', 'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-03-11 15:23:02', 'created' => '0000-00-00 00:00:00', 'select_label' => '325 - Flp polyclonal antibody (A280-004 - not determined - - Whole antiserum from rabbit containing 0.05% azide. - Rabbit)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/en/p/flp-polyclonal-antibody-pioneer-100-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'Flp polyclonal antibody - Pioneer (Sample Size)' $meta_title = 'Flp polyclonal antibody - Pioneer (Sample Size)' $product = array( 'Product' => array( 'id' => '3036', 'antibody_id' => '325', 'name' => 'Flp Antibody (Sample Size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. CS-169-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp</strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. CS-169-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15310169-20', 'old_catalog_number' => '', 'sf_code' => 'C15310169-361', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '/', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'flp-polyclonal-antibody-pioneer-20-ul-sample-size', 'meta_title' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'meta_keywords' => '', 'meta_description' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'modified' => '2021-12-20 14:10:44', 'created' => '2019-08-13 10:39:53', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '325', 'name' => 'Flp polyclonal antibody', 'description' => 'Flp recombinase originates from Saccharomyces cerevisiae and recognizes FRT sites in the genome. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-03-11 15:23:02', 'created' => '0000-00-00 00:00:00', 'select_label' => '325 - Flp polyclonal antibody (A280-004 - not determined - - Whole antiserum from rabbit containing 0.05% azide. - Rabbit)' ), 'Slave' => array(), 'Group' => array( 'Group' => array( 'id' => '267', 'name' => 'C15310169', 'product_id' => '2133', 'modified' => '2019-08-13 10:39:28', 'created' => '2019-08-13 10:39:28' ), 'Master' => array( 'id' => '2133', 'antibody_id' => '325', 'name' => 'Flp Antibody ', 'description' => '<p>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein <sup>(1)</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. C15310169) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp <sup>(1)</sup></strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. C15310169), diluted 1:500 in BSA/PBS-Tween. 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Classified based on level of validation for flexibility of application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode offers Antibodies exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-04 16:02:57', 'created' => '2014-12-25 12:45:50', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '102', 'position' => '1', 'parent_id' => '4', 'name' => 'Sample size antibodies', 'description' => '<h1><strong>Validated epigenetics antibodies</strong> – care for a sample?<br /> </h1> <p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p> <ul> <li><strong>Focused</strong> - Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li> <li><strong>Strict quality standards</strong> with rigorous QC and validation</li> <li><strong>Classified</strong> based on level of validation for flexibility of application</li> </ul> <p>Existing sample sizes are listed below. We will soon expand our collection. Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => true, 'is_antibody' => true, 'slug' => 'sample-size-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => '5-hmC monoclonal antibody,CRISPR/Cas9 polyclonal antibody ,H3K36me3 polyclonal antibody,diagenode', 'meta_description' => 'Diagenode offers sample volume on selected antibodies for researchers to test, validate and provide confidence and flexibility in choosing from our wide range of antibodies ', 'meta_title' => 'Sample-size Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:05', 'created' => '2015-10-27 12:13:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. 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Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>', 'image_id' => null, 'type' => 'Brochure', 'url' => 'files/brochures/Epigenetic_Antibodies_Brochure.pdf', 'slug' => 'epigenetic-antibodies-brochure', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-06-15 11:24:06', 'created' => '2015-07-03 16:05:27', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '429', 'name' => 'Datasheet Flp C15310169', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_Flp_CS-169-100.pdf', 'slug' => 'datasheet-flp-cs-169-100', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-03-05 14:25:51', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2181', 'name' => 'Development of a lentivirus vector-based assay for non-destructive monitoring of cell fusion activity.', 'authors' => 'Neshati Z, Liu J, Zhou G, Schalij MJ, de Vries AA', 'description' => '<p>Cell-to-cell fusion can be quantified by endowing acceptor and donor cells with latent reporter genes/proteins and activators of these genes/proteins, respectively. One way to accomplish this goal is by using a bipartite lentivirus vector (LV)-based cell fusion assay system in which the cellular fusion partners are transduced with a flippase-activatable Photinus pyralis luciferase (PpLuc) expression unit (acceptor cells) or with a recombinant gene encoding FLPeNLS+, a nuclear-targeted and molecularly evolved version of flippase (donor cells). Fusion of both cell populations will lead to the FLPe-dependent generation of a functional PpLuc gene. PpLuc activity is typically measured in cell lysates, precluding consecutive analysis of one cell culture. Therefore, in this study the PpLuc-coding sequence was replaced by that of Gaussia princeps luciferase (GpLuc), a secretory protein allowing repeated analysis of the same cell culture. In myotubes the spread of FLPeNLS+ may be limited due to its nuclear localization signal (NLS) causing low signal outputs. To test this hypothesis, myoblasts were transduced with LVs encoding either FLPeNLS+ or an NLS-less version of FLPe (FLPeNLS-) and subsequently co-cultured in different ratios with myoblasts containing the FLPe-activatable GpLuc expression cassette. At different times after induction of cell-to-cell fusion the GpLuc activity in the culture medium was determined. FLPeNLS+ and FLPeNLS- both activated the latent GpLuc gene but when the percentage of FLPe-expressing myoblasts was limiting, FLPeNLS+ generally yielded slightly higher signals than FLPeNLS- while at low acceptor-to-donor cell ratios FLPeNLS- was usually superior. The ability of FLPeNLS+ to spread through myofibers and to induce reporter gene expression is thus not limited by its NLS. However, at high FLPe concentrations the presence of the NLS negatively affected reporter gene expression. In summary, a rapid and simple chemiluminescence assay for quantifying cell-to-cell fusion progression based on GpLuc has been developed.</p>', 'date' => '2014-07-16', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25028973', 'doi' => '', 'modified' => '2016-04-11 10:35:35', 'created' => '2015-07-24 15:39:03', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '256', 'name' => 'Flp antibody SDS US en', 'language' => 'en', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2020-06-08 17:19:02', 'created' => '2020-06-08 17:19:02', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '254', 'name' => 'Flp antibody SDS GB en', 'language' => 'en', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2020-06-08 17:13:38', 'created' => '2020-06-08 17:13:38', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '249', 'name' => 'Flp antibody SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-BE-fr-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-08 17:09:23', 'created' => '2020-06-08 17:09:23', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '253', 'name' => 'Flp antibody SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-FR-fr-GHS_2_0.pdf', 'countries' => 'FR', 'modified' => '2020-06-08 17:13:04', 'created' => '2020-06-08 17:13:04', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '252', 'name' => 'Flp antibody SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-08 17:11:45', 'created' => '2020-06-08 17:11:45', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '251', 'name' => 'Flp antibody SDS DE de', 'language' => 'de', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-DE-de-GHS_2_0.pdf', 'countries' => 'DE', 'modified' => '2020-06-08 17:11:16', 'created' => '2020-06-08 17:11:16', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '255', 'name' => 'Flp antibody SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-06-08 17:18:31', 'created' => '2020-06-08 17:18:31', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '250', 'name' => 'Flp antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-08 17:10:18', 'created' => '2020-06-08 17:10:18', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/en/p/flp-polyclonal-antibody-pioneer-100-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2133', 'antibody_id' => '325', 'name' => 'Flp Antibody ', 'description' => '<p>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein <sup>(1)</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. C15310169) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp <sup>(1)</sup></strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. C15310169), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310169', 'old_catalog_number' => 'CS-169-100', 'sf_code' => 'C15310169-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 12, 2021', 'slug' => 'flp-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'Flp Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Flp (Flp recombinase) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website.', 'modified' => '2021-03-05 14:23:00', 'created' => '2015-06-29 14:08:20' ) ) $pro = array( 'id' => '3036', 'antibody_id' => '325', 'name' => 'Flp Antibody (Sample Size)', 'description' => '<p><span>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. CS-169-100) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp</strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. CS-169-100), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '20 µl', 'catalog_number' => 'C15310169-20', 'old_catalog_number' => '', 'sf_code' => 'C15310169-361', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '105', 'price_USD' => '115', 'price_GBP' => '100', 'price_JPY' => '16450', 'price_CNY' => '/', 'price_AUD' => '288', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '', 'slug' => 'flp-polyclonal-antibody-pioneer-20-ul-sample-size', 'meta_title' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'meta_keywords' => '', 'meta_description' => 'Flp polyclonal antibody - Pioneer (Sample Size)', 'modified' => '2021-12-20 14:10:44', 'created' => '2019-08-13 10:39:53', 'ProductsGroup' => array( 'id' => '302', 'product_id' => '3036', 'group_id' => '267' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(CS-169-100)</span>' $country_code = 'US' $other_format = array( 'id' => '2133', 'antibody_id' => '325', 'name' => 'Flp Antibody ', 'description' => '<p>Polyclonal antibody raised in rabbit against Flp recombinase using 3 KLH-conjugated synthetic peptides located at the N-terminal part of the protein <sup>(1)</sup>.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-elisa.png" alt="ELISA" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against Flp (cat. No. C15310169) in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.</small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310169-WB.png" alt="Western blot" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against Flp <sup>(1)</sup></strong><br />Western blot was performed on whole cell lysates from untransfected 293 cells (lane 1), or 293 cells transfected with Cre (lane 2), Dre (lane 3) or Flp (lane 4) with the Diagenode antibody against Flp (cat. No. C15310169), diluted 1:500 in BSA/PBS-Tween. The molecular weight marker (in KD) is shown on the left; the location of the protein of interest (expected size: 48 KD) is indicated on the right.</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '100 µl', 'catalog_number' => 'C15310169', 'old_catalog_number' => 'CS-169-100', 'sf_code' => 'C15310169-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '260', 'price_USD' => '260', 'price_GBP' => '245', 'price_JPY' => '40730', 'price_CNY' => '', 'price_AUD' => '650', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => 'February 12, 2021', 'slug' => 'flp-polyclonal-antibody-pioneer-100-ul', 'meta_title' => 'Flp Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'Flp (Flp recombinase) Polyclonal Antibody validated in WB and ELISA. Batch-specific data available on the website.', 'modified' => '2021-03-05 14:23:00', 'created' => '2015-06-29 14:08:20' ) $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'ProductsApplication' => array( 'id' => '5053', 'product_id' => '3036', 'application_id' => '20' ) ) $slugs = array( (int) 0 => 'elisa-antibodies' ) $applications = array( 'id' => '20', 'position' => '10', 'parent_id' => '40', 'name' => 'ELISA', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'elisa-antibodies', 'meta_keywords' => ' ELISA Antibodies,Monoclonal antibody, Polyclonal antibody', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for ELISA applications', 'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:21:41', 'created' => '2014-07-08 08:13:28', 'locale' => 'eng' ) $description = '<div class="row"> <div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div> </div>' $name = 'ELISA' $document = array( 'id' => '429', 'name' => 'Datasheet Flp C15310169', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_Flp_CS-169-100.pdf', 'slug' => 'datasheet-flp-cs-169-100', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2021-03-05 14:25:51', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '2788', 'product_id' => '3036', 'document_id' => '429' ) ) $sds = array( 'id' => '250', 'name' => 'Flp antibody SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/Flp/SDS-C15310169-Flp_Antibody-BE-nl-GHS_2_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-08 17:10:18', 'created' => '2020-06-08 17:10:18', 'ProductsSafetySheet' => array( 'id' => '475', 'product_id' => '3036', 'safety_sheet_id' => '250' ) ) $publication = array( 'id' => '2181', 'name' => 'Development of a lentivirus vector-based assay for non-destructive monitoring of cell fusion activity.', 'authors' => 'Neshati Z, Liu J, Zhou G, Schalij MJ, de Vries AA', 'description' => '<p>Cell-to-cell fusion can be quantified by endowing acceptor and donor cells with latent reporter genes/proteins and activators of these genes/proteins, respectively. One way to accomplish this goal is by using a bipartite lentivirus vector (LV)-based cell fusion assay system in which the cellular fusion partners are transduced with a flippase-activatable Photinus pyralis luciferase (PpLuc) expression unit (acceptor cells) or with a recombinant gene encoding FLPeNLS+, a nuclear-targeted and molecularly evolved version of flippase (donor cells). Fusion of both cell populations will lead to the FLPe-dependent generation of a functional PpLuc gene. PpLuc activity is typically measured in cell lysates, precluding consecutive analysis of one cell culture. Therefore, in this study the PpLuc-coding sequence was replaced by that of Gaussia princeps luciferase (GpLuc), a secretory protein allowing repeated analysis of the same cell culture. In myotubes the spread of FLPeNLS+ may be limited due to its nuclear localization signal (NLS) causing low signal outputs. To test this hypothesis, myoblasts were transduced with LVs encoding either FLPeNLS+ or an NLS-less version of FLPe (FLPeNLS-) and subsequently co-cultured in different ratios with myoblasts containing the FLPe-activatable GpLuc expression cassette. At different times after induction of cell-to-cell fusion the GpLuc activity in the culture medium was determined. FLPeNLS+ and FLPeNLS- both activated the latent GpLuc gene but when the percentage of FLPe-expressing myoblasts was limiting, FLPeNLS+ generally yielded slightly higher signals than FLPeNLS- while at low acceptor-to-donor cell ratios FLPeNLS- was usually superior. The ability of FLPeNLS+ to spread through myofibers and to induce reporter gene expression is thus not limited by its NLS. However, at high FLPe concentrations the presence of the NLS negatively affected reporter gene expression. In summary, a rapid and simple chemiluminescence assay for quantifying cell-to-cell fusion progression based on GpLuc has been developed.</p>', 'date' => '2014-07-16', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/25028973', 'doi' => '', 'modified' => '2016-04-11 10:35:35', 'created' => '2015-07-24 15:39:03', 'ProductsPublication' => array( 'id' => '4016', 'product_id' => '3036', 'publication_id' => '2181' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/25028973" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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