Monoclonal antibody raised in mouse against histone H3 dimethylated at lysine 36 (H3K36me2), using a KLH-conjugated synthetic peptide.
| Lot | 001-12 |
|---|---|
| Concentration | 1.0 µg/µl |
| Species reactivity | Human: positive. Other species: not tested. |
| Type | Monoclonal |
| Purity | Protein A purified monoclonal antibody. |
| Host | Mouse |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP * | 1-2 µg/ChIP | Fig 1 |
| ELISA | 1:3,000 | Fig 2 |
| Dot Blotting | 1:10,000 | |
| Western Blotting | 1:1,000 - 1:2,000 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.
Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K36me2
ChIP assays were performed using human HeLa cells, the Diagenode monoclonal antibody against H3K36me2 (Cat. No. C15200182) and optimized PCR primer pairs for qPCR. ChIP was performed with the “Auto Histone ChIP-seq” kit (Cat. No. C01010020), using sheared chromatin from 1 million cells. A titration consisting of 0.5, 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (1 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a genomic region upstream of the TGM2 gene, used as a positive control, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. Cross reactivity of the Diagenode monoclonal antibody directed against H3K36me2
To test the specificity an ELISA was performed using a serial dilution of the Diagenode monoclonal antibody against H3K36me2 (Cat. No. C15200182). The wells were coated with peptides containing the unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure 2 shows a high specificity of the antibody for the peptide containing the modification of interest.
 How to properly cite our product/service in your workWe strongly recommend using this: H3K36me2 Antibody (Hologic Diagenode Cat# C15200182 Lot# 001-12). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
Histone H3K36me2 and H3K36me3 form a chromatin platform essentialfor DNMT3A-dependent DNA methylation in mouse oocytes. |
A histone H3.3K36M mutation in mice causes an imbalance of histonemodifications and defects in chondrocyte differentiation. |
A lncRNA regulates alternative splicing via establishment of a splicing-specific chromatin signature. |
