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Polyclonal antibody raised in rabbit against Histone H3 (Monomethyl Lys37), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 0.42 μg/μl |
| Species reactivity | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Western Blotting | 1 μg/ml | Fig 1 |
| Dot Blotting | 1:1,000 | Fig 2, 3 |
Figure 1. Western Blot
Western Blot with the H3K37me1 antibody. Lane 1: HeLa histone extracts. Lane 2: NIH-3T3 histone extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at 1 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 2. Dot Blot
Dot Blot with the H3K37me1 antibody. Lane 1: K37me1. Lane 2: K36me1. Lane 3: K36me2. Lane 4: K36me3. Lane 5: K36ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
Figure 3. Dot Blot
Dot Blot with the H3K37me1 antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
| Datasheet H3K37me1 C15410295 DATASHEET Datasheet description | Download |
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SMYD5 is a histone H3-specific methyltransferase mediatingmono-methylation of histone H3 lysine 36 and 37. |
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Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-DB.png" alt="Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Dot Blot</strong><br /> Dot Blot with the H3K37me1 antibody. Lane 1: K37me1. Lane 2: K36me1. Lane 3: K36me2. Lane 4: K36me3. Lane 5: K36ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-DB2.png" alt="Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Dot Blot</strong><br /> Dot Blot with the H3K37me1 antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. 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Dot Blot</strong><br /> Dot Blot with the H3K37me1 antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' => '<p>Dot blotting</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'dot-blotting', 'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications', 'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 14:40:49', 'created' => '2015-07-08 13:45:05', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '111', 'position' => '40', 'parent_id' => '4', 'name' => 'Histone antibodies', 'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p> <p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p> <p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p> <p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p> <p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p> <p>Diagenode’s collection includes antibodies recognizing:</p> <ul> <li><strong>Histone H1 variants</strong></li> <li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li> <li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li> <li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li> <li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li> </ul> <p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p> <p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'histone-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Histone, antibody, histone h1, histone h2, histone h3, histone h4', 'meta_description' => 'Polyclonal and Monoclonal Antibodies against Histones and their modifications validated for many applications, including Chromatin Immunoprecipitation (ChIP) and ChIP-Sequencing (ChIP-seq)', 'meta_title' => 'Histone and Modified Histone Antibodies | Diagenode', 'modified' => '2020-09-17 13:34:56', 'created' => '2016-04-01 16:01:32', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. 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', 'description' => '<p>Although post-translational modifications (-PTMs) of some histone H3 lysine residues are well studied, the PTMs of histone H3 lysine 37 in mammalian cells remain largely unknown. In this study, we provide evidence to show that SMYD family member 5 (SMYD5) is a histone H3-specfic methyltransferase that catalyzes mono-methylation of H3 lysine 36 and 37 (H3K36/K37me1) in vitro. The site-mutagenesis analysis shows that a species-conserved histidine in its catalytic SET domain is required for its histone methyltransferase activity. Genetic deletion of Smyd5 in murine embryonic stem cells (mESCs) partially reduces the global histone H3K37me1 level in cells, suggesting SMYD5 is one of histone methyltransferases catalyzing histone H3K37me1 in vivo. Hence, our study reveals that SMYD5 is a histone H3-specific methyltransferase that mediates histone H3K36/K37me1, which provides a biochemical basis for further studying its functions in mammalian cells.</p>', 'date' => '2022-04-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35182940', 'doi' => '10.1016/j.bbrc.2022.02.043', 'modified' => '2022-11-24 09:29:49', 'created' => '2022-11-24 08:49:52', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' => '<p>Dot blotting</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'dot-blotting', 'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications', 'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 14:40:49', 'created' => '2015-07-08 13:45:05', 'ProductsApplication' => array( 'id' => '2451', 'product_id' => '2414', 'application_id' => '28' ) ) $slugs = array( (int) 0 => 'dot-blotting' ) $applications = array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' => '<p>Dot blotting</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'dot-blotting', 'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications', 'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 14:40:49', 'created' => '2015-07-08 13:45:05', 'locale' => 'eng' ) $description = '<p>Dot blotting</p>' $name = 'DB' $document = array( 'id' => '506', 'name' => 'Datasheet H3K37me1 C15410295', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H3K37me1_C15410295.pdf', 'slug' => 'datasheet-h3k37me1-c15410295', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '390', 'product_id' => '2414', 'document_id' => '506' ) ) $publication = array( 'id' => '4555', 'name' => 'SMYD5 is a histone H3-specific methyltransferase mediatingmono-methylation of histone H3 lysine 36 and 37.', 'authors' => 'Aljazi Mohammad B. et al. ', 'description' => '<p>Although post-translational modifications (-PTMs) of some histone H3 lysine residues are well studied, the PTMs of histone H3 lysine 37 in mammalian cells remain largely unknown. In this study, we provide evidence to show that SMYD family member 5 (SMYD5) is a histone H3-specfic methyltransferase that catalyzes mono-methylation of H3 lysine 36 and 37 (H3K36/K37me1) in vitro. The site-mutagenesis analysis shows that a species-conserved histidine in its catalytic SET domain is required for its histone methyltransferase activity. Genetic deletion of Smyd5 in murine embryonic stem cells (mESCs) partially reduces the global histone H3K37me1 level in cells, suggesting SMYD5 is one of histone methyltransferases catalyzing histone H3K37me1 in vivo. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'H3K37me1 (Histone H3 monomethylated at Lysine 37) Polyclonal Antibody validated in WB and DB. ', 'meta_title' => 'H3K37me1 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2414', 'antibody_id' => '356', 'name' => 'H3K37me1 Antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against Histone H3 (Monomethyl Lys37), using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. Western Blot</strong><br /> Western Blot with the H3K37me1 antibody. Lane 1: HeLa histone extracts. Lane 2: NIH-3T3 histone extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at 1 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-DB.png" alt="Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Dot Blot</strong><br /> Dot Blot with the H3K37me1 antibody. Lane 1: K37me1. Lane 2: K36me1. Lane 3: K36me2. Lane 4: K36me3. Lane 5: K36ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-DB2.png" alt="Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Dot Blot</strong><br /> Dot Blot with the H3K37me1 antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. 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' $meta_title = 'H3K37me1 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2414', 'antibody_id' => '356', 'name' => 'H3K37me1 Antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against Histone H3 (Monomethyl Lys37), using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. Western Blot</strong><br /> Western Blot with the H3K37me1 antibody. Lane 1: HeLa histone extracts. Lane 2: NIH-3T3 histone extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at 1 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-DB.png" alt="Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Dot Blot</strong><br /> Dot Blot with the H3K37me1 antibody. Lane 1: K37me1. Lane 2: K36me1. Lane 3: K36me2. Lane 4: K36me3. Lane 5: K36ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-DB2.png" alt="Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Dot Blot</strong><br /> Dot Blot with the H3K37me1 antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. 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A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p> <p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p> <p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p> <p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p> <p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p> <p>Diagenode’s collection includes antibodies recognizing:</p> <ul> <li><strong>Histone H1 variants</strong></li> <li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li> <li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li> <li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li> <li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li> </ul> <p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p> <p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'histone-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Histone, antibody, histone h1, histone h2, histone h3, histone h4', 'meta_description' => 'Polyclonal and Monoclonal Antibodies against Histones and their modifications validated for many applications, including Chromatin Immunoprecipitation (ChIP) and ChIP-Sequencing (ChIP-seq)', 'meta_title' => 'Histone and Modified Histone Antibodies | Diagenode', 'modified' => '2020-09-17 13:34:56', 'created' => '2016-04-01 16:01:32', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. 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', 'description' => '<p>Although post-translational modifications (-PTMs) of some histone H3 lysine residues are well studied, the PTMs of histone H3 lysine 37 in mammalian cells remain largely unknown. In this study, we provide evidence to show that SMYD family member 5 (SMYD5) is a histone H3-specfic methyltransferase that catalyzes mono-methylation of H3 lysine 36 and 37 (H3K36/K37me1) in vitro. The site-mutagenesis analysis shows that a species-conserved histidine in its catalytic SET domain is required for its histone methyltransferase activity. Genetic deletion of Smyd5 in murine embryonic stem cells (mESCs) partially reduces the global histone H3K37me1 level in cells, suggesting SMYD5 is one of histone methyltransferases catalyzing histone H3K37me1 in vivo. Hence, our study reveals that SMYD5 is a histone H3-specific methyltransferase that mediates histone H3K36/K37me1, which provides a biochemical basis for further studying its functions in mammalian cells.</p>', 'date' => '2022-04-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/35182940', 'doi' => '10.1016/j.bbrc.2022.02.043', 'modified' => '2022-11-24 09:29:49', 'created' => '2022-11-24 08:49:52', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $application = array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' => '<p>Dot blotting</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'dot-blotting', 'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications', 'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 14:40:49', 'created' => '2015-07-08 13:45:05', 'ProductsApplication' => array( 'id' => '2451', 'product_id' => '2414', 'application_id' => '28' ) ) $slugs = array( (int) 0 => 'dot-blotting' ) $applications = array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' => '<p>Dot blotting</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'dot-blotting', 'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications', 'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 14:40:49', 'created' => '2015-07-08 13:45:05', 'locale' => 'eng' ) $description = '<p>Dot blotting</p>' $name = 'DB' $document = array( 'id' => '506', 'name' => 'Datasheet H3K37me1 C15410295', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_H3K37me1_C15410295.pdf', 'slug' => 'datasheet-h3k37me1-c15410295', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( 'id' => '390', 'product_id' => '2414', 'document_id' => '506' ) ) $publication = array( 'id' => '4555', 'name' => 'SMYD5 is a histone H3-specific methyltransferase mediatingmono-methylation of histone H3 lysine 36 and 37.', 'authors' => 'Aljazi Mohammad B. et al. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'H3K37me1 (Histone H3 monomethylated at Lysine 37) Polyclonal Antibody validated in WB and DB. ', 'meta_title' => 'H3K37me1 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2414', 'antibody_id' => '356', 'name' => 'H3K37me1 Antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against Histone H3 (Monomethyl Lys37), using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. Western Blot</strong><br /> Western Blot with the H3K37me1 antibody. Lane 1: HeLa histone extracts. Lane 2: NIH-3T3 histone extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at 1 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-DB.png" alt="Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Dot Blot</strong><br /> Dot Blot with the H3K37me1 antibody. Lane 1: K37me1. Lane 2: K36me1. Lane 3: K36me2. Lane 4: K36me3. Lane 5: K36ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-DB2.png" alt="Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Dot Blot</strong><br /> Dot Blot with the H3K37me1 antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. 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Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-DB.png" alt="Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Dot Blot</strong><br /> Dot Blot with the H3K37me1 antibody. Lane 1: K37me1. Lane 2: K36me1. Lane 3: K36me2. Lane 4: K36me3. Lane 5: K36ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-DB2.png" alt="Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Dot Blot</strong><br /> Dot Blot with the H3K37me1 antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' => '<p>Dot blotting</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'dot-blotting', 'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications', 'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 14:40:49', 'created' => '2015-07-08 13:45:05', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '111', 'position' => '40', 'parent_id' => '4', 'name' => 'Histone antibodies', 'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p> <p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p> <p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p> <p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p> <p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p> <p>Diagenode’s collection includes antibodies recognizing:</p> <ul> <li><strong>Histone H1 variants</strong></li> <li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li> <li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li> <li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li> <li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li> </ul> <p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p> <p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'histone-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Histone, antibody, histone h1, histone h2, histone h3, histone h4', 'meta_description' => 'Polyclonal and Monoclonal Antibodies against Histones and their modifications validated for many applications, including Chromatin Immunoprecipitation (ChIP) and ChIP-Sequencing (ChIP-seq)', 'meta_title' => 'Histone and Modified Histone Antibodies | Diagenode', 'modified' => '2020-09-17 13:34:56', 'created' => '2016-04-01 16:01:32', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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', 'description' => '<p>Although post-translational modifications (-PTMs) of some histone H3 lysine residues are well studied, the PTMs of histone H3 lysine 37 in mammalian cells remain largely unknown. In this study, we provide evidence to show that SMYD family member 5 (SMYD5) is a histone H3-specfic methyltransferase that catalyzes mono-methylation of H3 lysine 36 and 37 (H3K36/K37me1) in vitro. The site-mutagenesis analysis shows that a species-conserved histidine in its catalytic SET domain is required for its histone methyltransferase activity. Genetic deletion of Smyd5 in murine embryonic stem cells (mESCs) partially reduces the global histone H3K37me1 level in cells, suggesting SMYD5 is one of histone methyltransferases catalyzing histone H3K37me1 in vivo. 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'H3K37me1 (Histone H3 monomethylated at Lysine 37) Polyclonal Antibody validated in WB and DB. ', 'meta_title' => 'H3K37me1 Polyclonal Antibody | Diagenode', 'product' => array( 'Product' => array( 'id' => '2414', 'antibody_id' => '356', 'name' => 'H3K37me1 Antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against Histone H3 (Monomethyl Lys37), using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. Western Blot</strong><br /> Western Blot with the H3K37me1 antibody. Lane 1: HeLa histone extracts. Lane 2: NIH-3T3 histone extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at 1 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-DB.png" alt="Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Dot Blot</strong><br /> Dot Blot with the H3K37me1 antibody. Lane 1: K37me1. Lane 2: K36me1. Lane 3: K36me2. Lane 4: K36me3. Lane 5: K36ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-DB2.png" alt="Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Dot Blot</strong><br /> Dot Blot with the H3K37me1 antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410295', 'old_catalog_number' => '', 'sf_code' => 'C15410295-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '475', 'price_USD' => '480', 'price_GBP' => '435', 'price_JPY' => '74410', 'price_CNY' => '', 'price_AUD' => '1200', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k37me1-polyclonal-antibody-classic-50-mg', 'meta_title' => 'H3K37me1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'H3K37me1 (Histone H3 monomethylated at Lysine 37) Polyclonal Antibody validated in WB and DB. 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Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '0.42 μg/μl ', 'reactivity' => 'Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Western Blotting</td> <td>1 μg/ml</td> <td>Fig 1</td> </tr> <tr> <td>Dot Blotting</td> <td>1:1,000</td> <td>Fig 2, 3</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 15:38:42', 'created' => '2015-07-17 17:20:11', 'select_label' => '356 - H3K37me1 polyclonal antibody - Classic (001 - 0.42 μg/μl - Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'en' $meta_keywords = '' $meta_description = 'H3K37me1 (Histone H3 monomethylated at Lysine 37) Polyclonal Antibody validated in WB and DB. ' $meta_title = 'H3K37me1 Polyclonal Antibody | Diagenode' $product = array( 'Product' => array( 'id' => '2414', 'antibody_id' => '356', 'name' => 'H3K37me1 Antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against Histone H3 (Monomethyl Lys37), using a KLH-conjugated synthetic peptide.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 1. Western Blot</strong><br /> Western Blot with the H3K37me1 antibody. Lane 1: HeLa histone extracts. Lane 2: NIH-3T3 histone extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at 1 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-DB.png" alt="Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 2. Dot Blot</strong><br /> Dot Blot with the H3K37me1 antibody. Lane 1: K37me1. Lane 2: K36me1. Lane 3: K36me2. Lane 4: K36me3. Lane 5: K36ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410295-DB2.png" alt="Dot Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small><strong> Figure 3. Dot Blot</strong><br /> Dot Blot with the H3K37me1 antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT. </small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 μg', 'catalog_number' => 'C15410295', 'old_catalog_number' => '', 'sf_code' => 'C15410295-D001-000581', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '475', 'price_USD' => '480', 'price_GBP' => '435', 'price_JPY' => '74410', 'price_CNY' => '', 'price_AUD' => '1200', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'h3k37me1-polyclonal-antibody-classic-50-mg', 'meta_title' => 'H3K37me1 Polyclonal Antibody | Diagenode', 'meta_keywords' => '', 'meta_description' => 'H3K37me1 (Histone H3 monomethylated at Lysine 37) Polyclonal Antibody validated in WB and DB. ', 'modified' => '2022-01-05 15:40:27', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => '356', 'name' => 'H3K37me1 polyclonal antibody - Classic', 'description' => 'Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine.', 'clonality' => '', 'isotype' => '', 'lot' => '001', 'concentration' => '0.42 μg/μl ', 'reactivity' => 'Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>Western Blotting</td> <td>1 μg/ml</td> <td>Fig 1</td> </tr> <tr> <td>Dot Blotting</td> <td>1:1,000</td> <td>Fig 2, 3</td> </tr> </tbody> </table>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 15:38:42', 'created' => '2015-07-17 17:20:11', 'select_label' => '356 - H3K37me1 polyclonal antibody - Classic (001 - 0.42 μg/μl - Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '28', 'position' => '10', 'parent_id' => '40', 'name' => 'DB', 'description' => '<p>Dot blotting</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'dot-blotting', 'meta_keywords' => 'Dot blotting,Monoclonal & Polyclonal antibody,', 'meta_description' => 'Diagenode offers Monoclonal & Polyclonal antibodies for Dot blotting applications', 'meta_title' => 'Dot blotting Antibodies - Monoclonal & Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 14:40:49', 'created' => '2015-07-08 13:45:05', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '111', 'position' => '40', 'parent_id' => '4', 'name' => 'Histone antibodies', 'description' => '<p>Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A <strong>nucleosome</strong> consists of one pair of each of the core histones (<strong>H2A</strong>, <strong>H2B</strong>, <strong>H3</strong> and <strong>H4</strong>) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone<strong> H1, </strong>allowing for further condensation of chromatin.</p> <p>The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These <strong>post-translational modifications </strong>include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are <strong>methylation,</strong> <strong>acetylation and phosphorylation</strong>. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).</p> <p>The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with <strong>euchromatin</strong> and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in <strong>heterochromatin </strong>and associated with gene silencing. The combination of different histone modifications is called the "<strong>histone code</strong>”, analogous to the genetic code.</p> <p><img src="https://www.diagenode.com/img/categories/antibodies/histone-marks-illustration.png" /></p> <p>Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including <strong>ChIP</strong> and <strong>ChIP-seq</strong>.</p> <p>Diagenode’s collection includes antibodies recognizing:</p> <ul> <li><strong>Histone H1 variants</strong></li> <li><strong>Histone H2A, H2A variants and histone H2A</strong> <strong>modifications</strong> (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)</li> <li><strong>Histone H2B and H2B</strong> <strong>modifications </strong>(serine phosphorylation, lysine acetylation)</li> <li><strong>Histone H3 and H3 modifications </strong>(lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))</li> <li><strong>Histone H4 and H4 modifications (</strong>lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )</li> </ul> <p><span style="font-weight: 400;"><strong>HDAC's HAT's, HMT's and other</strong> <strong>enzymes</strong> which modify histones can be found in the category <a href="../categories/chromatin-modifying-proteins-histone-transferase">Histone modifying enzymes</a><br /></span></p> <p><span style="font-weight: 400;"> Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'histone-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Histone, antibody, histone h1, histone h2, histone h3, histone h4', 'meta_description' => 'Polyclonal and Monoclonal Antibodies against Histones and their modifications validated for many applications, including Chromatin Immunoprecipitation (ChIP) and ChIP-Sequencing (ChIP-seq)', 'meta_title' => 'Histone and Modified Histone Antibodies | Diagenode', 'modified' => '2020-09-17 13:34:56', 'created' => '2016-04-01 16:01:32', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. 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