Polyclonal antibody raised in rabbit against Histone H3 (Monomethyl Lys37), using a KLH-conjugated synthetic peptide.
| Lot | 001 |
|---|---|
| Concentration | 0.42 μg/μl |
| Species reactivity | Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| Western Blotting | 1 μg/ml | Fig 1 |
| Dot Blotting | 1:1,000 | Fig 2, 3 |
Figure 1. Western Blot
Western Blot with the H3K37me1 antibody. Lane 1: HeLa histone extracts. Lane 2: NIH-3T3 histone extracts. Lane 3: C. elegans embryo lysate. Load: 30 μg per lane. Primary antibody used at 1 μg/ml overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 2. Dot Blot
Dot Blot with the H3K37me1 antibody. Lane 1: K37me1. Lane 2: K36me1. Lane 3: K36me2. Lane 4: K36me3. Lane 5: K36ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
Figure 3. Dot Blot
Dot Blot with the H3K37me1 antibody. Lane 1: K37. Lane 2: K37Ac. Lane 3: K37Me1. Lane 4: K37Me2. Lane 5: K37Me3. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 1:1000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
 How to properly cite our product/service in your workWe strongly recommend using this: H3K37me1 Antibody (Hologic Diagenode Cat# C15410295 Lot# 001). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
SMYD5 is a histone H3-specific methyltransferase mediatingmono-methylation of histone H3 lysine 36 and 37. |
