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Monoclonal antibody raised in rabbit against the region of histone H3 containing the dimethylated lysine 9 (H3K9me2), using a KLH-conjugated synthetic peptide.
Lot
001
Concentration
1 µg/µl
Species reactivity
Human: positive Other species: not tested
Type
Monoclonal ChIP-grade
Purity
Affinity purified monoclonal antibody
Host
Rabbit
Storage Conditions
Store at -20°C for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage Buffer
PBS containing 50% glycerol, 1% BSA and 0.09% azide
Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications
Suggested dilution
References
ChIP *
0.5 µg per IP
Fig 1
Western Blotting
1:1,000
Fig 2
Immunofluorescence
1:500
Fig 3
*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5 - 5 µg per IP.
Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K9me2 ChIP was performed with the Diagenode antibody against H3K9me2 (cat. No. C15210019) on sheared chromatin from 500,000 HeLaS3 cells using the “iDeal ChIP-seq” kit (cat. No. C01010051). A titration of the antibody consisting of 0.5, 1, and 2 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the HBB promoter and the MYOD1 gene, used as positive controls, and for the EIF4A2 and GAPDH promoters, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against H3K9me2 Western blot was performed on whole cell extracts (40 µg) from HeLa cells using the Diagenode antibody against H3K9me2 (cat. No. C15210019). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.
Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against H3K9me2 HeLa cells were stained with the Diagenode antibody against H3K9me2 (cat. No. C15210019, red) diluted 1:500. Actin was stained with fluorescein phalladoin (green).
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
Learn more about: Load... Read more
IF Immunofluorescence:
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
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