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Monoclonal antibody raised in rabbit against the region of histone H4 containing the monomethylated arginine 3 (H4R3me1), using a KLH-conjugated synthetic peptide.
Lot
001
Concentration
1 µg/µl
Species reactivity
Human: positive. Other species: not tested.
Type
Monoclonal
Purity
Affinity purified monoclonal antibody
Host
Rabbit
Storage Conditions
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage Buffer
PBS containing 50% glycerol, 1% BSA and 0.09% azide.
Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Figure 1. Cross reactivity tests using the Diagenode monoclonal antibody directed against H4R3me1 To test the cross reactivity of the Diagenode antibody against H3K18ac (cat. No. C15210022), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4R3. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Figure 3 shows a high specificity of the antibody for the modification of interest.
Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against H4R3me1 Western blot was performed on whole cell extracts (40 µg) from HeLa cells using the Diagenode antibody against H4R3me1 (cat. No. C15210022). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is shown on the right, the marker (in kDa) is shown on the left.
Figure 3. Immunofluorescence using the Diagenode monoclonal antibody directed against H4R3me1 HeLa cells were stained with the Diagenode antibody against H3K18ac (cat. No. C15210020, red) diluted 1:500. Actin was stained with fluorescein phalladoin (green).
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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IF Immunofluorescence:
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
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