Datasheet Human GAPDH promoter pp1003 DATASHEET Datasheet description | Download |
These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).
Datasheet Human GAPDH promoter pp1003 DATASHEET Datasheet description | Download |
How to properly cite this product in your workDiagenode strongly recommends using this: Human GAPDH promoter -1.0 kb primer pair (Diagenode Cat# C17011003-50). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Evaluating the Feasibility of DNA Methylation Analyses Using Long-Term Archived Brain Formalin-Fixed Paraffin-Embedded Samples |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'product' => array( 'Product' => array( 'id' => '2515', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011003-50', 'old_catalog_number' => 'pp-1003-050', 'sf_code' => 'C17011003-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2015-09-02 11:31:36', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/en/p/human-gapdh-promoter-1-0-kb-primer-pair-50-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'Human GAPDH promoter -1.0 kb primer pair' $meta_title = 'Human GAPDH promoter -1.0 kb primer pair' $product = array( 'Product' => array( 'id' => '2515', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011003-50', 'old_catalog_number' => 'pp-1003-050', 'sf_code' => 'C17011003-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2015-09-02 11:31:36', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '133', 'name' => 'C17011003', 'product_id' => '2515', 'modified' => '2016-02-19 21:38:47', 'created' => '2016-02-19 21:38:47' ) ), 'Group' => array( 'Group' => array( 'id' => '133', 'name' => 'C17011003', 'product_id' => '2515', 'modified' => '2016-02-19 21:38:47', 'created' => '2016-02-19 21:38:47' ), 'Master' => array( 'id' => '2515', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011003-50', 'old_catalog_number' => 'pp-1003-050', 'sf_code' => 'C17011003-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2015-09-02 11:31:36', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '69', 'position' => '1', 'parent_id' => '35', 'name' => 'Human', 'description' => '<p>Primer pairs - Human</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'primer-pairs-human', 'cookies_tag_id' => null, 'meta_keywords' => 'Primer pairs, human, ChIP-seq grade', 'meta_description' => 'Diagenode offers Primer pairs for Human ChIP-seq grade', 'meta_title' => 'Primer pairs - Human | Diagenode', 'modified' => '2016-10-21 08:00:50', 'created' => '2015-07-23 15:38:42', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '236', 'name' => 'Datasheet Human GAPDH promoter pp1003', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_promoter_pp1003.pdf', 'slug' => 'datasheet-human-gapdh-promoter-pp1003', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3108', 'name' => 'Evaluating the Feasibility of DNA Methylation Analyses Using Long-Term Archived Brain Formalin-Fixed Paraffin-Embedded Samples', 'authors' => 'Bak S.T. et al.', 'description' => '<p>We here characterize the usability of archival formalin-fixed paraffin-embedded (FFPE) brain tissue as a resource for genetic and DNA methylation analyses with potential relevance for brain-manifested diseases. We analyzed FFPE samples from The Brain Collection, Aarhus University Hospital Risskov, Denmark (AUBC), constituting 9479 formalin-fixated brains making it one of the largest collections worldwide. DNA extracted from brain FFPE tissue blocks was interrogated for quality and usability in genetic and DNA methylation analyses by different molecular techniques. Overall, we found that DNA quality was inversely correlated with storage time and DNA quality was insufficient for Illumina methylation arrays; data from methylated DNA immunoprecipitation, clonal bisulfite sequencing, and pyrosequencing of BDNF and ST6GALNAC1 suggested that the original methylation pattern is indeed preserved. Proof-of-principle experiments predicting sex based on the methylation status of the X-inactivated SLC9A7 gene, or genotype differences of the Y and X chromosomes, showed consistency between predicted and actual sex for a subset of FFPE samples. In conclusion, even though DNA from FFPE samples is of low quality and technically challenging, it is likely that a subset of samples can provide reliable data given that the methodology used is designed for small DNA fragments. We propose that simple PCR-based quality control experiments at the genetic and DNA methylation level, carried out at the beginning of any given project, can be used to enrich for the best-performing FFPE samples. The apparent preservation of genetic and DNA methylation patterns in archival FFPE samples may bring along new perspectives for the identification of genetic and epigenetic changes associated with brain-manifested diseases.</p>', 'date' => '2016-12-19', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/27995571', 'doi' => '', 'modified' => '2017-01-04 10:19:32', 'created' => '2017-01-04 10:19:32', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/human-gapdh-promoter-1-0-kb-primer-pair-50-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2516', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011003-500', 'old_catalog_number' => 'pp-1003-500', 'sf_code' => 'C17011003-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-500-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2016-02-19 21:38:56', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '144', 'product_id' => '2516', 'group_id' => '133' ) ) ) $pro = array( 'id' => '2516', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011003-500', 'old_catalog_number' => 'pp-1003-500', 'sf_code' => 'C17011003-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-500-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2016-02-19 21:38:56', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '144', 'product_id' => '2516', 'group_id' => '133' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(pp-1003-500)</span>' $country_code = 'US' $other_format = array( 'id' => '2516', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011003-500', 'old_catalog_number' => 'pp-1003-500', 'sf_code' => 'C17011003-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-500-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2016-02-19 21:38:56', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '144', 'product_id' => '2516', 'group_id' => '133' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '236', 'name' => 'Datasheet Human GAPDH promoter pp1003', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_promoter_pp1003.pdf', 'slug' => 'datasheet-human-gapdh-promoter-pp1003', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '605', 'product_id' => '2515', 'document_id' => '236' ) ) $publication = array( 'id' => '3108', 'name' => 'Evaluating the Feasibility of DNA Methylation Analyses Using Long-Term Archived Brain Formalin-Fixed Paraffin-Embedded Samples', 'authors' => 'Bak S.T. et al.', 'description' => '<p>We here characterize the usability of archival formalin-fixed paraffin-embedded (FFPE) brain tissue as a resource for genetic and DNA methylation analyses with potential relevance for brain-manifested diseases. We analyzed FFPE samples from The Brain Collection, Aarhus University Hospital Risskov, Denmark (AUBC), constituting 9479 formalin-fixated brains making it one of the largest collections worldwide. DNA extracted from brain FFPE tissue blocks was interrogated for quality and usability in genetic and DNA methylation analyses by different molecular techniques. Overall, we found that DNA quality was inversely correlated with storage time and DNA quality was insufficient for Illumina methylation arrays; data from methylated DNA immunoprecipitation, clonal bisulfite sequencing, and pyrosequencing of BDNF and ST6GALNAC1 suggested that the original methylation pattern is indeed preserved. Proof-of-principle experiments predicting sex based on the methylation status of the X-inactivated SLC9A7 gene, or genotype differences of the Y and X chromosomes, showed consistency between predicted and actual sex for a subset of FFPE samples. In conclusion, even though DNA from FFPE samples is of low quality and technically challenging, it is likely that a subset of samples can provide reliable data given that the methodology used is designed for small DNA fragments. We propose that simple PCR-based quality control experiments at the genetic and DNA methylation level, carried out at the beginning of any given project, can be used to enrich for the best-performing FFPE samples. The apparent preservation of genetic and DNA methylation patterns in archival FFPE samples may bring along new perspectives for the identification of genetic and epigenetic changes associated with brain-manifested diseases.</p>', 'date' => '2016-12-19', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/27995571', 'doi' => '', 'modified' => '2017-01-04 10:19:32', 'created' => '2017-01-04 10:19:32', 'ProductsPublication' => array( 'id' => '1818', 'product_id' => '2515', 'publication_id' => '3108' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/27995571" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'product' => array( 'Product' => array( 'id' => '2515', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011003-50', 'old_catalog_number' => 'pp-1003-050', 'sf_code' => 'C17011003-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2015-09-02 11:31:36', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/en/p/human-gapdh-promoter-1-0-kb-primer-pair-50-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'Human GAPDH promoter -1.0 kb primer pair' $meta_title = 'Human GAPDH promoter -1.0 kb primer pair' $product = array( 'Product' => array( 'id' => '2515', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011003-50', 'old_catalog_number' => 'pp-1003-050', 'sf_code' => 'C17011003-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2015-09-02 11:31:36', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '133', 'name' => 'C17011003', 'product_id' => '2515', 'modified' => '2016-02-19 21:38:47', 'created' => '2016-02-19 21:38:47' ) ), 'Group' => array( 'Group' => array( 'id' => '133', 'name' => 'C17011003', 'product_id' => '2515', 'modified' => '2016-02-19 21:38:47', 'created' => '2016-02-19 21:38:47' ), 'Master' => array( 'id' => '2515', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011003-50', 'old_catalog_number' => 'pp-1003-050', 'sf_code' => 'C17011003-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2015-09-02 11:31:36', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '69', 'position' => '1', 'parent_id' => '35', 'name' => 'Human', 'description' => '<p>Primer pairs - Human</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'primer-pairs-human', 'cookies_tag_id' => null, 'meta_keywords' => 'Primer pairs, human, ChIP-seq grade', 'meta_description' => 'Diagenode offers Primer pairs for Human ChIP-seq grade', 'meta_title' => 'Primer pairs - Human | Diagenode', 'modified' => '2016-10-21 08:00:50', 'created' => '2015-07-23 15:38:42', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '236', 'name' => 'Datasheet Human GAPDH promoter pp1003', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_promoter_pp1003.pdf', 'slug' => 'datasheet-human-gapdh-promoter-pp1003', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3108', 'name' => 'Evaluating the Feasibility of DNA Methylation Analyses Using Long-Term Archived Brain Formalin-Fixed Paraffin-Embedded Samples', 'authors' => 'Bak S.T. et al.', 'description' => '<p>We here characterize the usability of archival formalin-fixed paraffin-embedded (FFPE) brain tissue as a resource for genetic and DNA methylation analyses with potential relevance for brain-manifested diseases. We analyzed FFPE samples from The Brain Collection, Aarhus University Hospital Risskov, Denmark (AUBC), constituting 9479 formalin-fixated brains making it one of the largest collections worldwide. DNA extracted from brain FFPE tissue blocks was interrogated for quality and usability in genetic and DNA methylation analyses by different molecular techniques. Overall, we found that DNA quality was inversely correlated with storage time and DNA quality was insufficient for Illumina methylation arrays; data from methylated DNA immunoprecipitation, clonal bisulfite sequencing, and pyrosequencing of BDNF and ST6GALNAC1 suggested that the original methylation pattern is indeed preserved. Proof-of-principle experiments predicting sex based on the methylation status of the X-inactivated SLC9A7 gene, or genotype differences of the Y and X chromosomes, showed consistency between predicted and actual sex for a subset of FFPE samples. In conclusion, even though DNA from FFPE samples is of low quality and technically challenging, it is likely that a subset of samples can provide reliable data given that the methodology used is designed for small DNA fragments. We propose that simple PCR-based quality control experiments at the genetic and DNA methylation level, carried out at the beginning of any given project, can be used to enrich for the best-performing FFPE samples. The apparent preservation of genetic and DNA methylation patterns in archival FFPE samples may bring along new perspectives for the identification of genetic and epigenetic changes associated with brain-manifested diseases.</p>', 'date' => '2016-12-19', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/27995571', 'doi' => '', 'modified' => '2017-01-04 10:19:32', 'created' => '2017-01-04 10:19:32', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/human-gapdh-promoter-1-0-kb-primer-pair-50-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2516', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011003-500', 'old_catalog_number' => 'pp-1003-500', 'sf_code' => 'C17011003-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-500-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2016-02-19 21:38:56', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '144', 'product_id' => '2516', 'group_id' => '133' ) ) ) $pro = array( 'id' => '2516', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011003-500', 'old_catalog_number' => 'pp-1003-500', 'sf_code' => 'C17011003-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-500-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2016-02-19 21:38:56', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '144', 'product_id' => '2516', 'group_id' => '133' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(pp-1003-500)</span>' $country_code = 'US' $other_format = array( 'id' => '2516', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011003-500', 'old_catalog_number' => 'pp-1003-500', 'sf_code' => 'C17011003-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-500-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2016-02-19 21:38:56', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '144', 'product_id' => '2516', 'group_id' => '133' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '236', 'name' => 'Datasheet Human GAPDH promoter pp1003', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_promoter_pp1003.pdf', 'slug' => 'datasheet-human-gapdh-promoter-pp1003', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '605', 'product_id' => '2515', 'document_id' => '236' ) ) $publication = array( 'id' => '3108', 'name' => 'Evaluating the Feasibility of DNA Methylation Analyses Using Long-Term Archived Brain Formalin-Fixed Paraffin-Embedded Samples', 'authors' => 'Bak S.T. et al.', 'description' => '<p>We here characterize the usability of archival formalin-fixed paraffin-embedded (FFPE) brain tissue as a resource for genetic and DNA methylation analyses with potential relevance for brain-manifested diseases. We analyzed FFPE samples from The Brain Collection, Aarhus University Hospital Risskov, Denmark (AUBC), constituting 9479 formalin-fixated brains making it one of the largest collections worldwide. DNA extracted from brain FFPE tissue blocks was interrogated for quality and usability in genetic and DNA methylation analyses by different molecular techniques. Overall, we found that DNA quality was inversely correlated with storage time and DNA quality was insufficient for Illumina methylation arrays; data from methylated DNA immunoprecipitation, clonal bisulfite sequencing, and pyrosequencing of BDNF and ST6GALNAC1 suggested that the original methylation pattern is indeed preserved. Proof-of-principle experiments predicting sex based on the methylation status of the X-inactivated SLC9A7 gene, or genotype differences of the Y and X chromosomes, showed consistency between predicted and actual sex for a subset of FFPE samples. In conclusion, even though DNA from FFPE samples is of low quality and technically challenging, it is likely that a subset of samples can provide reliable data given that the methodology used is designed for small DNA fragments. We propose that simple PCR-based quality control experiments at the genetic and DNA methylation level, carried out at the beginning of any given project, can be used to enrich for the best-performing FFPE samples. The apparent preservation of genetic and DNA methylation patterns in archival FFPE samples may bring along new perspectives for the identification of genetic and epigenetic changes associated with brain-manifested diseases.</p>', 'date' => '2016-12-19', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/27995571', 'doi' => '', 'modified' => '2017-01-04 10:19:32', 'created' => '2017-01-04 10:19:32', 'ProductsPublication' => array( 'id' => '1818', 'product_id' => '2515', 'publication_id' => '3108' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/27995571" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'product' => array( 'Product' => array( 'id' => '2515', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011003-50', 'old_catalog_number' => 'pp-1003-050', 'sf_code' => 'C17011003-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2015-09-02 11:31:36', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/en/p/human-gapdh-promoter-1-0-kb-primer-pair-50-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'Human GAPDH promoter -1.0 kb primer pair' $meta_title = 'Human GAPDH promoter -1.0 kb primer pair' $product = array( 'Product' => array( 'id' => '2515', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011003-50', 'old_catalog_number' => 'pp-1003-050', 'sf_code' => 'C17011003-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2015-09-02 11:31:36', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '133', 'name' => 'C17011003', 'product_id' => '2515', 'modified' => '2016-02-19 21:38:47', 'created' => '2016-02-19 21:38:47' ) ), 'Group' => array( 'Group' => array( 'id' => '133', 'name' => 'C17011003', 'product_id' => '2515', 'modified' => '2016-02-19 21:38:47', 'created' => '2016-02-19 21:38:47' ), 'Master' => array( 'id' => '2515', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011003-50', 'old_catalog_number' => 'pp-1003-050', 'sf_code' => 'C17011003-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2015-09-02 11:31:36', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '69', 'position' => '1', 'parent_id' => '35', 'name' => 'Human', 'description' => '<p>Primer pairs - Human</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'primer-pairs-human', 'cookies_tag_id' => null, 'meta_keywords' => 'Primer pairs, human, ChIP-seq grade', 'meta_description' => 'Diagenode offers Primer pairs for Human ChIP-seq grade', 'meta_title' => 'Primer pairs - Human | Diagenode', 'modified' => '2016-10-21 08:00:50', 'created' => '2015-07-23 15:38:42', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '236', 'name' => 'Datasheet Human GAPDH promoter pp1003', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_promoter_pp1003.pdf', 'slug' => 'datasheet-human-gapdh-promoter-pp1003', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3108', 'name' => 'Evaluating the Feasibility of DNA Methylation Analyses Using Long-Term Archived Brain Formalin-Fixed Paraffin-Embedded Samples', 'authors' => 'Bak S.T. et al.', 'description' => '<p>We here characterize the usability of archival formalin-fixed paraffin-embedded (FFPE) brain tissue as a resource for genetic and DNA methylation analyses with potential relevance for brain-manifested diseases. We analyzed FFPE samples from The Brain Collection, Aarhus University Hospital Risskov, Denmark (AUBC), constituting 9479 formalin-fixated brains making it one of the largest collections worldwide. DNA extracted from brain FFPE tissue blocks was interrogated for quality and usability in genetic and DNA methylation analyses by different molecular techniques. Overall, we found that DNA quality was inversely correlated with storage time and DNA quality was insufficient for Illumina methylation arrays; data from methylated DNA immunoprecipitation, clonal bisulfite sequencing, and pyrosequencing of BDNF and ST6GALNAC1 suggested that the original methylation pattern is indeed preserved. Proof-of-principle experiments predicting sex based on the methylation status of the X-inactivated SLC9A7 gene, or genotype differences of the Y and X chromosomes, showed consistency between predicted and actual sex for a subset of FFPE samples. In conclusion, even though DNA from FFPE samples is of low quality and technically challenging, it is likely that a subset of samples can provide reliable data given that the methodology used is designed for small DNA fragments. We propose that simple PCR-based quality control experiments at the genetic and DNA methylation level, carried out at the beginning of any given project, can be used to enrich for the best-performing FFPE samples. The apparent preservation of genetic and DNA methylation patterns in archival FFPE samples may bring along new perspectives for the identification of genetic and epigenetic changes associated with brain-manifested diseases.</p>', 'date' => '2016-12-19', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/27995571', 'doi' => '', 'modified' => '2017-01-04 10:19:32', 'created' => '2017-01-04 10:19:32', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/human-gapdh-promoter-1-0-kb-primer-pair-50-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2516', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011003-500', 'old_catalog_number' => 'pp-1003-500', 'sf_code' => 'C17011003-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-500-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2016-02-19 21:38:56', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '144', 'product_id' => '2516', 'group_id' => '133' ) ) ) $pro = array( 'id' => '2516', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011003-500', 'old_catalog_number' => 'pp-1003-500', 'sf_code' => 'C17011003-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-500-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2016-02-19 21:38:56', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '144', 'product_id' => '2516', 'group_id' => '133' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(pp-1003-500)</span>' $country_code = 'US' $other_format = array( 'id' => '2516', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011003-500', 'old_catalog_number' => 'pp-1003-500', 'sf_code' => 'C17011003-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-500-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2016-02-19 21:38:56', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '144', 'product_id' => '2516', 'group_id' => '133' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '236', 'name' => 'Datasheet Human GAPDH promoter pp1003', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_promoter_pp1003.pdf', 'slug' => 'datasheet-human-gapdh-promoter-pp1003', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '605', 'product_id' => '2515', 'document_id' => '236' ) ) $publication = array( 'id' => '3108', 'name' => 'Evaluating the Feasibility of DNA Methylation Analyses Using Long-Term Archived Brain Formalin-Fixed Paraffin-Embedded Samples', 'authors' => 'Bak S.T. et al.', 'description' => '<p>We here characterize the usability of archival formalin-fixed paraffin-embedded (FFPE) brain tissue as a resource for genetic and DNA methylation analyses with potential relevance for brain-manifested diseases. We analyzed FFPE samples from The Brain Collection, Aarhus University Hospital Risskov, Denmark (AUBC), constituting 9479 formalin-fixated brains making it one of the largest collections worldwide. DNA extracted from brain FFPE tissue blocks was interrogated for quality and usability in genetic and DNA methylation analyses by different molecular techniques. Overall, we found that DNA quality was inversely correlated with storage time and DNA quality was insufficient for Illumina methylation arrays; data from methylated DNA immunoprecipitation, clonal bisulfite sequencing, and pyrosequencing of BDNF and ST6GALNAC1 suggested that the original methylation pattern is indeed preserved. Proof-of-principle experiments predicting sex based on the methylation status of the X-inactivated SLC9A7 gene, or genotype differences of the Y and X chromosomes, showed consistency between predicted and actual sex for a subset of FFPE samples. In conclusion, even though DNA from FFPE samples is of low quality and technically challenging, it is likely that a subset of samples can provide reliable data given that the methodology used is designed for small DNA fragments. We propose that simple PCR-based quality control experiments at the genetic and DNA methylation level, carried out at the beginning of any given project, can be used to enrich for the best-performing FFPE samples. The apparent preservation of genetic and DNA methylation patterns in archival FFPE samples may bring along new perspectives for the identification of genetic and epigenetic changes associated with brain-manifested diseases.</p>', 'date' => '2016-12-19', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/27995571', 'doi' => '', 'modified' => '2017-01-04 10:19:32', 'created' => '2017-01-04 10:19:32', 'ProductsPublication' => array( 'id' => '1818', 'product_id' => '2515', 'publication_id' => '3108' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/27995571" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'en', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'product' => array( 'Product' => array( 'id' => '2515', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011003-50', 'old_catalog_number' => 'pp-1003-050', 'sf_code' => 'C17011003-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2015-09-02 11:31:36', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/en/p/human-gapdh-promoter-1-0-kb-primer-pair-50-ul' ) $language = 'en' $meta_keywords = '' $meta_description = 'Human GAPDH promoter -1.0 kb primer pair' $meta_title = 'Human GAPDH promoter -1.0 kb primer pair' $product = array( 'Product' => array( 'id' => '2515', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011003-50', 'old_catalog_number' => 'pp-1003-050', 'sf_code' => 'C17011003-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2015-09-02 11:31:36', 'created' => '2015-06-29 14:08:20', 'locale' => 'eng' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '133', 'name' => 'C17011003', 'product_id' => '2515', 'modified' => '2016-02-19 21:38:47', 'created' => '2016-02-19 21:38:47' ) ), 'Group' => array( 'Group' => array( 'id' => '133', 'name' => 'C17011003', 'product_id' => '2515', 'modified' => '2016-02-19 21:38:47', 'created' => '2016-02-19 21:38:47' ), 'Master' => array( 'id' => '2515', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011003-50', 'old_catalog_number' => 'pp-1003-050', 'sf_code' => 'C17011003-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-50-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2015-09-02 11:31:36', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '69', 'position' => '1', 'parent_id' => '35', 'name' => 'Human', 'description' => '<p>Primer pairs - Human</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'primer-pairs-human', 'cookies_tag_id' => null, 'meta_keywords' => 'Primer pairs, human, ChIP-seq grade', 'meta_description' => 'Diagenode offers Primer pairs for Human ChIP-seq grade', 'meta_title' => 'Primer pairs - Human | Diagenode', 'modified' => '2016-10-21 08:00:50', 'created' => '2015-07-23 15:38:42', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '236', 'name' => 'Datasheet Human GAPDH promoter pp1003', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_promoter_pp1003.pdf', 'slug' => 'datasheet-human-gapdh-promoter-pp1003', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3108', 'name' => 'Evaluating the Feasibility of DNA Methylation Analyses Using Long-Term Archived Brain Formalin-Fixed Paraffin-Embedded Samples', 'authors' => 'Bak S.T. et al.', 'description' => '<p>We here characterize the usability of archival formalin-fixed paraffin-embedded (FFPE) brain tissue as a resource for genetic and DNA methylation analyses with potential relevance for brain-manifested diseases. We analyzed FFPE samples from The Brain Collection, Aarhus University Hospital Risskov, Denmark (AUBC), constituting 9479 formalin-fixated brains making it one of the largest collections worldwide. DNA extracted from brain FFPE tissue blocks was interrogated for quality and usability in genetic and DNA methylation analyses by different molecular techniques. Overall, we found that DNA quality was inversely correlated with storage time and DNA quality was insufficient for Illumina methylation arrays; data from methylated DNA immunoprecipitation, clonal bisulfite sequencing, and pyrosequencing of BDNF and ST6GALNAC1 suggested that the original methylation pattern is indeed preserved. Proof-of-principle experiments predicting sex based on the methylation status of the X-inactivated SLC9A7 gene, or genotype differences of the Y and X chromosomes, showed consistency between predicted and actual sex for a subset of FFPE samples. In conclusion, even though DNA from FFPE samples is of low quality and technically challenging, it is likely that a subset of samples can provide reliable data given that the methodology used is designed for small DNA fragments. We propose that simple PCR-based quality control experiments at the genetic and DNA methylation level, carried out at the beginning of any given project, can be used to enrich for the best-performing FFPE samples. The apparent preservation of genetic and DNA methylation patterns in archival FFPE samples may bring along new perspectives for the identification of genetic and epigenetic changes associated with brain-manifested diseases.</p>', 'date' => '2016-12-19', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/27995571', 'doi' => '', 'modified' => '2017-01-04 10:19:32', 'created' => '2017-01-04 10:19:32', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/human-gapdh-promoter-1-0-kb-primer-pair-50-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2516', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011003-500', 'old_catalog_number' => 'pp-1003-500', 'sf_code' => 'C17011003-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-500-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2016-02-19 21:38:56', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '144', 'product_id' => '2516', 'group_id' => '133' ) ) ) $pro = array( 'id' => '2516', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011003-500', 'old_catalog_number' => 'pp-1003-500', 'sf_code' => 'C17011003-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-500-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2016-02-19 21:38:56', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '144', 'product_id' => '2516', 'group_id' => '133' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(pp-1003-500)</span>' $country_code = 'US' $other_format = array( 'id' => '2516', 'antibody_id' => null, 'name' => 'Human GAPDH promoter -1.0 kb primer pair', 'description' => '<p><span>These primers are specific to a DNA region located 1.0kb upstream (-1.0kb) from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011003-500', 'old_catalog_number' => 'pp-1003-500', 'sf_code' => 'C17011003-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-gapdh-promoter-1-0-kb-primer-pair-500-ul', 'meta_title' => 'Human GAPDH promoter -1.0 kb primer pair', 'meta_keywords' => '', 'meta_description' => 'Human GAPDH promoter -1.0 kb primer pair', 'modified' => '2016-02-19 21:38:56', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '144', 'product_id' => '2516', 'group_id' => '133' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '236', 'name' => 'Datasheet Human GAPDH promoter pp1003', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_GAPDH_promoter_pp1003.pdf', 'slug' => 'datasheet-human-gapdh-promoter-pp1003', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '605', 'product_id' => '2515', 'document_id' => '236' ) ) $publication = array( 'id' => '3108', 'name' => 'Evaluating the Feasibility of DNA Methylation Analyses Using Long-Term Archived Brain Formalin-Fixed Paraffin-Embedded Samples', 'authors' => 'Bak S.T. et al.', 'description' => '<p>We here characterize the usability of archival formalin-fixed paraffin-embedded (FFPE) brain tissue as a resource for genetic and DNA methylation analyses with potential relevance for brain-manifested diseases. We analyzed FFPE samples from The Brain Collection, Aarhus University Hospital Risskov, Denmark (AUBC), constituting 9479 formalin-fixated brains making it one of the largest collections worldwide. DNA extracted from brain FFPE tissue blocks was interrogated for quality and usability in genetic and DNA methylation analyses by different molecular techniques. Overall, we found that DNA quality was inversely correlated with storage time and DNA quality was insufficient for Illumina methylation arrays; data from methylated DNA immunoprecipitation, clonal bisulfite sequencing, and pyrosequencing of BDNF and ST6GALNAC1 suggested that the original methylation pattern is indeed preserved. Proof-of-principle experiments predicting sex based on the methylation status of the X-inactivated SLC9A7 gene, or genotype differences of the Y and X chromosomes, showed consistency between predicted and actual sex for a subset of FFPE samples. In conclusion, even though DNA from FFPE samples is of low quality and technically challenging, it is likely that a subset of samples can provide reliable data given that the methodology used is designed for small DNA fragments. We propose that simple PCR-based quality control experiments at the genetic and DNA methylation level, carried out at the beginning of any given project, can be used to enrich for the best-performing FFPE samples. The apparent preservation of genetic and DNA methylation patterns in archival FFPE samples may bring along new perspectives for the identification of genetic and epigenetic changes associated with brain-manifested diseases.</p>', 'date' => '2016-12-19', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/27995571', 'doi' => '', 'modified' => '2017-01-04 10:19:32', 'created' => '2017-01-04 10:19:32', 'ProductsPublication' => array( 'id' => '1818', 'product_id' => '2515', 'publication_id' => '3108' ) ) $externalLink = ' <a href="https://www.ncbi.nlm.nih.gov/pubmed/27995571" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
To ensure you see the information most relevant to you, please select your country. Please note that your browser will need to be configured to accept cookies.
Diagenode will process your personal data in strict accordance with its privacy policy. This will include sending you updates about us, our products, and resources we think would be of interest to you.