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These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. These primers can be used to amplify DNA isolated by chromatin immunoprecipitation (ChIP). Primers are optimized to be used in quantitative polymerase chain reaction (qPCR).
| Datasheet Human GAPDH pp1001 DATASHEET Datasheet description | Download |
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Occupancy of RNA Polymerase II Phosphorylated on Serine 5 (RNAP S5P) and RNAP S2P on Varicella-Zoster Virus Genes 9, 51, and 66 Is Independent of Transcript Abundance and Polymerase Location within the Gene |
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In contrast, phosphorylated serine 2 (S2(P))-modified RNAP was not detected at any virus gene location at 3 dpi and was detected at levels only slightly above background levels at 1 dpi.</p>', 'date' => '2015-11-11', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26559844', 'doi' => '10.1128/JVI.02617-15', 'modified' => '2016-06-09 10:12:13', 'created' => '2016-06-09 10:12:13', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $meta_canonical = 'https://www.diagenode.com/en/p/human-gapdh-promoter-tata-box-primer-pair-50-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2513', 'antibody_id' => null, 'name' => 'Human GAPDH promoter (TATA-box) primer pair', 'description' => '<p><span>These primers are specific to a DNA region of the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. 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Chromatin immunoprecipitation (ChIP) is widely used to determine the location of specific proteins along a genomic region. Since the size range of sheared virus DNA fragments governs the limit of accurate protein localization, particularly for compact herpesvirus genomes, we used a quantitative PCR (qPCR)-based assay to determine the efficiency of VZV DNA shearing before ChIP, after which the assay was used to determine the relationship between transcript abundance and the occupancy of phosphorylated RNA polymerase II (RNAP) on the gene promoter, body, and terminus of VZV genes 9, 51, and 66. The abundance of VZV gene 9, 51, and 66 transcripts in VZV-infected human fetal lung fibroblasts was determined by reverse transcription-linked quantitative PCR. Our results showed that the C-terminal domain of RNAP is hyperphosphorylated at serine 5 (S5(P)) on VZV genes 9, 51, and 66 independently of transcript abundance and the location within the virus gene at both 1 and 3 days postinfection (dpi). In contrast, phosphorylated serine 2 (S2(P))-modified RNAP was not detected at any virus gene location at 3 dpi and was detected at levels only slightly above background levels at 1 dpi.</p>', 'date' => '2015-11-11', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26559844', 'doi' => '10.1128/JVI.02617-15', 'modified' => '2016-06-09 10:12:13', 'created' => '2016-06-09 10:12:13', 'ProductsPublication' => array( 'id' => '1320', 'product_id' => '2514', 'publication_id' => '2947' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26559844" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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